Predicted mutation
evidence seq id position mutation freq annotation gene description
RA minE 231,703:1 +C 100% intergenic (+155/+84) thrW → / ← hemB tRNA‑Thr/porphobilinogen synthase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*minE231,7031.C87.5% 12.3 / ‑2.4 8intergenic (+155/+84)thrW/hemBtRNA‑Thr/porphobilinogen synthase
Reads supporting (aligned to +/- strand):  ref base . (1/0);  new base C (7/0);  total (8/0)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.
Rejected as polymorphism: Polymorphic indel expands or contracts a homopolymer stretch.

CAATCTTATCTACTGACCAAAAAGGCCTGATAGGTAATACGAGAGTGGACGGTCCCCTCG‑CCCCCTTGGGGAGAGGGTTAGGGTGAGGGGGCGTTCACCGTACTTTC  >  minE/231644‑231750
                                                            |                                               
cAATCTTATCTACTGACCAAAAAGGCCTGATAGGTAATACGAGAGTGGACGGTCCCCTCG‑CCCCCTTg                                         >  1:1463802/1‑68 (MQ=255)
                                       cGAGAGTGGACGGTCCCCTCGCCCCCCTTGGGGAGAGGGTTAGGGTGAGGGGGCGTTCACCGTACTTTc  >  1:1498780/1‑69 (MQ=255)
                                       cGAGAGTGGACGGTCCCCTCGCCCCCCTTGGGGAGAGGGTTAGGGTGAGGGGGCGTTCACCGTACTTTc  >  1:2615753/1‑69 (MQ=255)
                                       cGAGAGTGGACGGTCCCCTCGCCCCCCTTGGGGAGAGGGTTAGGGTGAGGGGGCGTTCACCGTACTTTc  >  1:2924497/1‑69 (MQ=255)
                                       cGAGAGTGGACGGTCCCCTCGCCCCCCTTGGGGAGAGGGTTAGGGTGAGGGGGCGTTCACCGTACTTTc  >  1:36099/1‑69 (MQ=255)
                                       cGAGAGTGGACGGTCCCCTCGCCCCCCTTGGGGAGAGGGTTAGGGTGAGGGGGCGTTCACCGTACTTTc  >  1:361393/1‑69 (MQ=255)
                                       cGAGAGTGGACGGTCCCCTCGCCCCCCTTGGGGAGAGGGTTAGGGTGAGGGGGCGTTCACCGTACTTTc  >  1:42487/1‑69 (MQ=255)
                                       cGAGAGTGGACGGTCCCCTCGCCCCCCTTGGGGAGAGGGTTAGGGTGAGGGGGCGTTCACCGTACTTTc  >  1:438506/1‑69 (MQ=255)
                                                            |                                               
CAATCTTATCTACTGACCAAAAAGGCCTGATAGGTAATACGAGAGTGGACGGTCCCCTCG‑CCCCCTTGGGGAGAGGGTTAGGGTGAGGGGGCGTTCACCGTACTTTC  >  minE/231644‑231750

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑