| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 67,547 | C→A | 34.6% | L241L (CTG→CTT) | leuA ← | 2‑isopropylmalate synthase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 67,547 | 0 | C | A | 34.6% | 132.0 / 87.9 | 124 | L241L (CTG→CTT) | leuA | 2‑isopropylmalate synthase |
| Reads supporting (aligned to +/- strand): ref base C (48/33); new base A (10/33); total (58/66) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.45e-04 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.76e-01 | |||||||||||
Only 100 of 124 total aligned reads displayed.
TGATTAATGGCGGTGTGGACGTTGAGAATATCCTTACGAACTTTGATCGCCATGATGACTTCTTCCAGGGAACAGTTTCCGGCACGCTCGCCGATCCCGTTCATTGCGCCTTCCACCTGGCGTGC > minE/67482‑67606 | tgattgatGGCGGTGTGGACGTTGAGAATATCCTTACGAACTTTGATCGCCATGATGACTTCTTCAAg > 1:405529/1‑68 (MQ=255)tgattgatGGCGGTGTGGACGTTGAGAATATCCTTACGAACTTTGATCGCCATGATGACTTCTTCAAg > 1:1806816/1‑68 (MQ=255)tgattgatGGCGGTGTGGACGTTGAGAATATCCTTACGAACTTTGATCGCCATGATGACTTCTTCAAg > 1:2497304/1‑68 (MQ=255)tgattgatGGCGGTGTGGACGTTGAGAATATCCTTACGAACTTTGATCGCCATGATGACTTCTTCAAg > 1:618998/1‑68 (MQ=255)tgattgatGGCGGTGTGGACGTTGAGAATATCCTTACGAACTTTGATCGCCATGATGACTTCTTCAAg > 1:950702/1‑68 (MQ=255)tgattgatGGCGGTGTGGACGTTGAGAATATCCTTACGAACTTTGATCGCCATGATGACTTCTTCAAg > 1:1078893/1‑68 (MQ=255)tgattgatGGCGGTGTGGACGTTGAGAATATCCTTACGAACTTTGATCGCCATGATGACTTCCTCAAg > 1:2697892/1‑68 (MQ=255) cGGTGTGGACGTTGAGAATATCCTTACGAACTTTGATCGCCATTATGACTTCTTCAAggg < 1:3283545/60‑1 (MQ=255) cGGTGTGGACGTTGAGAATATCCTTACGAACTTTGATCGCCATGATGACTTCTTCAAggg < 1:3305621/60‑1 (MQ=255) cGGTGTGGACGTTGAGAATATCCTTACGAACTTTGATCGCCATGATGACTTCTTCAAggg < 1:1234374/60‑1 (MQ=255) cGGTGTGGACGTTGAGAATATCCTTACGAACTTTGATCGCCATGATGACTTCTTCAAggg < 1:3235026/60‑1 (MQ=255) cGGTGTGGACGTTGAGAATATCCTTACGAACTTTGATCGCCATGATGACTTCTTCAAggg < 1:3005506/60‑1 (MQ=255) cGGTGTGGACGTTGAGAATATCCTTACGAACTTTGATCGCCATGATGACTTCTTCAAggg < 1:296133/60‑1 (MQ=255) cGGTGTGGACGTTGAGAATATCCTTACGAACTTTGATCGCCATGATGACTTCTTCAAggg < 1:894389/60‑1 (MQ=255) cGGTGTGGACGTTGAGAATATCCTTACGAACTTTGATCGCCATGATGACTTCTTCAAggg < 1:1534184/60‑1 (MQ=255) cGGTGTGGACGTTGAGAATATCCTTACGAACTTTGATCGCCATGATGACTTCTTCAAggg < 1:1581705/60‑1 (MQ=255) cGGTGTGGACGTTGAGAATATCCTTACGAACTTTGATCGCCATGATGACTTCTTCAAggg < 1:878539/60‑1 (MQ=255) cGGTGTGGACGTTGAGAATATCCTTACGAACTTTGATCGCCATGATGACTTCTTCAAggg < 1:2540149/60‑1 (MQ=255) cGGTGTGGACGTTGAGAATATCCTTACGAACTTTGATCGCCATGATGACTTCTTCAAggg < 1:591509/60‑1 (MQ=255) gtgtGGACGTTGAGAATATCCTTACGAACTTTGATCGCCATGATGACTTCTTCCAGGGAACAGTTTcc > 1:367693/1‑68 (MQ=255) gtgtGGACGTTGAGAATATCCTTACGAACTTTGATCGCCATGATGACTTCTTCCAGGGAACAGTTTcc > 1:2293618/1‑68 (MQ=255) gtgtGGACGTTGAGAATATCCTTACGAACTTTGATCGCCATGATGACTTCTTCCAGGGAACAGTTTcc > 1:2628740/1‑68 (MQ=255) gtgtGGACGTTGAGAATATCCTTACGAACTTTGATCGCCATGATGACTTCTTCCAGGGAACAGTTTcc > 1:1002746/1‑68 (MQ=255) gtgtGGACGTTGAGAATATCCTTACGAACTTTGATCGCCATGATGACTTCTTCCAGGGAACAGTTTcc > 1:3229904/1‑68 (MQ=255) gtgtGGACGTTGAGAATATCCTTACGAACTTTGATCGCCATGATGACTTCTTCCAGGGAACAGTTTcc > 1:3377294/1‑68 (MQ=255) gtgtGGACGTTGAGAATATCCTTACGAACTTTGATCGCCATGATGACTTCTTCCAGGGAACAGTTTcc > 1:129941/1‑68 (MQ=255) gtgtGGACGTTGAGAATATCCTTACGAACTTTGATCGCCATGATGACTTCTTCCAGGGAACAGTTTcc > 1:1491021/1‑68 (MQ=255) gtgGACGTTGAGAATATCCTTACGAACTTTGATCGCCATGATGACTTCTTCCAGGGAACAGTTTCCGGc > 1:412612/1‑69 (MQ=255) gtgGACGTTGAGAATATCCTTACGAACTTTGATCGCCATGATGACTTCTTCCAGGGAACAGTTTCCGGc > 1:3019332/1‑69 (MQ=255) gtgGACGTTGAGAATATCCTTACGAACTTTGATCGCCATGATGACTTCTTCCAGGGAACAGTTTCCGGc > 1:704983/1‑69 (MQ=255) gtgGACGTTGAGAATATCCTTACGAACTTTGATCGCCATGATGACTTCTTCCAGGGAACAGTTTCCGGc > 1:2771185/1‑69 (MQ=255) gtgGACGTTGAGAATATCCTTACGAACTTTGATCGCCATGATGACTTCTTCCAGGGAACAGTTTCCGGc > 1:2690841/1‑69 (MQ=255) gtgGACGTTGAGAATATCCTTACGAACTTTGATCGCCATGATGACTTCTTCCAGGGAACAGTTTCCGGc > 1:2661112/1‑69 (MQ=255) tgGACGTTGAGAATATCCTTACGAACTTTGATCGCCATGATGACTTCTTCCAGGGAACAGTTTCCGGCa > 1:432736/1‑69 (MQ=255) gACGTTGAGAATATCCTTACGAACTTTGATCGCCATGATGACTTCTTCCAGGGAACAGTTTCCGGCACg > 1:542293/1‑69 (MQ=255) gACGTTGAGAATATCCTTACGAACTTTGATCGCCATGATGACTTCTTCCAGGGAACAGTTTCCGGCACg > 1:3299078/1‑69 (MQ=255) gACGTTGAGAATATCCTTACGAACTTTGATCGCCATGATGACTTCTTCCAGGGAACAGTTTCCGGCACg > 1:3206876/1‑69 (MQ=255) gACGTTGAGAATATCCTTACGAACTTTGATCGCCATGATGACTTCTTCCAGGGAACAGTTTCCGGCACg > 1:3052791/1‑69 (MQ=255) gACGTTGAGAATATCCTTACGAACTTTGATCGCCATGATGACTTCTTCCAGGGAACAGTTTCCGGCACg > 1:1750295/1‑69 (MQ=255) gACGTTGAGAATATCCTTACGAACTTTGATCGCCATGATGACTTCTTCCAGGGAACAGTTTCCGGCACg > 1:1880855/1‑69 (MQ=255) gACGTTGAGAATATCCTTACGAACTTTGATCGCCATGATGACTTCTTCCAGGGAACAGTTTCCGGCACg < 1:1932499/69‑1 (MQ=255) aCGTTGAGAATATCCTTACGAACTTTGATCGCCATGATGACTTCTTCCAGGGAACAGTTTCCGGCACg < 1:601452/68‑1 (MQ=255) aaTATCCTTACGAACTTTGATCGCCATGATGACTTCTTCCAGGGAACAGTTTCCGGCACGCTCGCCGa < 1:968827/68‑1 (MQ=255) aaTATCCTTACGAACTTTGATCGCCATGATGACTTCTTCCAGGGAACAGTTTCCGGCACGCTCGCCGa < 1:3128733/68‑1 (MQ=255) aaTATCCTTACGAACTTTGATCGCCATGATGACTTCTTCCAGGGAACAGTTTCCGGCACGCTCGCCGa < 1:3059014/68‑1 (MQ=255) aaTATCCTTACGAACTTTGATCGCCATGATGACTTCTTCCAGGGAACAGTTTCCGGCACGCTCGCCGa < 1:2922694/68‑1 (MQ=255) aaTATCCTTACGAACTTTGATCGCCATGATGACTTCTTCCAGGGAACAGTTTCCGGCACGCTCGCCGa < 1:3330948/68‑1 (MQ=255) atatCCTTACGAACTTTGATCGCCATGATGACTTCTTCAAggg < 1:3298593/43‑1 (MQ=255) atCCTTACGAACTTTGATCGCCATGATGACTTCTTCCAGGGAACAGTTTCCGGCACGCTCGCCGATccc > 1:739724/1‑69 (MQ=255) atCCTTACGAACTTTGATCGCCATGATGACTTCTTCCAGGGAACAGTTTCCGGCACGCTCGCCGATccc > 1:1497132/1‑69 (MQ=255) atCCTTACGAACTTTGATCGCCATGATGACTTCTTCCAGGGAACAGTTTCCGGCACGCTCGCCGATccc > 1:2429024/1‑69 (MQ=255) atCCTTACGAACTTTGATCGCCATGATGACTTCTTCCAGGGAACAGTTTCCGGCACGCTCGCCGATccc > 1:83014/1‑69 (MQ=255) tCCTTACGAACTTTGATCGCCATGATGACTTCTTCCAGGGAACAGTTTCCgg > 1:2866572/1‑52 (MQ=255) tCCTTACGAACTTTGATCGCCATGATGACTTCTTCCAGGGAACAGTTTCCgg > 1:12082/1‑52 (MQ=255) tCCTTACGAACTTTGATCGCCATGATGACTTCTTCCAGGGAACAGTTTCCgg > 1:2220433/1‑52 (MQ=255) tCCTTACGAACTTTGATCGCCATGATGACTTCTTCCAGGGAACAGTTTCCGGCACGCTCGCCGATCCCg > 1:2113900/1‑69 (MQ=255) tCCTTACGAACTTTGATCGCCATGATGACTTCTTCCAGGGAACAGTTTCCGGCACGCTCGCCGATCCCg > 1:3396930/1‑69 (MQ=255) tCCTTACGAACTTTGATCGCCATGATGACTTCTTCCAGGGAACAGTTTCCGGCACGCTCGCCGATCCCg > 1:179998/1‑69 (MQ=255) tCCTTACGAACTTTGATCGCCATGATGACTTCTTCCAGGGAACAGTTTCCGGCACGCTCGCCGATCCCg > 1:2667056/1‑69 (MQ=255) tCCTTACGAACTTTGATCGCCATGATGACTTCTTCCAGGGAACAGTTTCCGGCACGCTCGCCGATCCCg > 1:2892937/1‑69 (MQ=255) cTTACGAACTTTGATCGCCATGATGACTTCTTCCAGGGAACAGTTTCCGGCACGCTCGCCGATCCCGtt < 1:2500462/69‑1 (MQ=255) cTTACGAACTTTGATCGCCATGATGACTTCTTCCAGGGAACAGTTTCCGGCACGCTCGCCGATCCCGtt < 1:126302/69‑1 (MQ=255) cTTACGAACTTTGAACGCCATGATGACTTCTTCCAGGGAACAGTTTCCGGCACGCTCGCCGATCCCGtt < 1:2327423/69‑1 (MQ=255) aaCTTTGATCGCCATGATGACTTCTTCCAGGGATCAGTTTCCGGCACGCTCGCCGATCCCGTTc < 1:2774063/64‑1 (MQ=255) aaCTTTGATCGCCATGATGACTTCTTCCAGGGAACAGTTTCCGGCACGCTCGCCGATCCCGTTc < 1:2042153/64‑1 (MQ=255) aaCTTTGATCGCCATGATGACTTCTTCCAGGGAACAGTTTCCGGCACGCTCGCCGATCCCGTTc < 1:528073/64‑1 (MQ=255) aaCTTTGATCGCCATGATGACTTCTTCCAGGGAACAGTTTCCGGCACGCTCGCCGATCCCGTTc < 1:173919/64‑1 (MQ=255) cTTTGATCGCCATGATGACTTCTTCCAGGGAACAGTTTCCGGCACGCTCGCCGATCCCGTTCATTgcgc < 1:1152235/69‑1 (MQ=255) cTTTGATCGCCATGATGACTTCTTCCAGGGAACAGTTTCCGGCACGCTCGCCGATCCCGTTCATTgcgc < 1:2140627/69‑1 (MQ=255) cTTTGATCGCCATGATGACTTCTTCCAGGGAACAGTTTCCGGCACGCTCGCCGATCCCGTTCATTgcgc < 1:909538/69‑1 (MQ=255) cTTTGATCGCCATGATGACTTCTTCCAGGGAACAGTTTCCGGCACGCTCGCCGATCCCGTTCATTgcgc < 1:549249/69‑1 (MQ=255) cTTTGATCGCCATGATGACTTCTTCCAGGGAACAGTTTCCGGCACGCTCGCCGATCCCGTTCATTgcgc < 1:553250/69‑1 (MQ=255) tttGATCGCCATGATGACTTCTTCCAGGGAACAGTTTCCGGCACGCTCGCCGATCCCGTTCATTgcgc < 1:2142219/68‑1 (MQ=255) tttGATCGCCATGATGACTTCTTCAAGGGAACAGTTACCGGCACGCTCGCCGATCCCGTTCATTGCGcc < 1:950812/69‑1 (MQ=255) tttGATCGCCATGATGACTTCTTCAAGGGAACAGTTACCGGCACGCTCGCCGATCCCGTTCATTGCGcc < 1:389390/69‑1 (MQ=255) tttGATCGCCATGATGACTTCTTCAAGGGAACAGTTACCGGCACGCTCGCCGATCCCGTTCATTGCGcc < 1:2815759/69‑1 (MQ=255) tttGATCGCCATGATGACTTCTTCAAGGGAACAGTTACCGGCACGCTCGCCGATCCCGTTCATTGCGcc < 1:3384217/69‑1 (MQ=255) tttGATCGCCATGATGACTTCTTCAAGGGAACAGTTACCGGCACGCTCGCCGATCCCGTTCATTGCGcc < 1:1020664/69‑1 (MQ=255) tttGATCGCCATGATGACTTCTTCAAGGGAACAGTTACCGGCACGCTCGCCGATCCCGTTCATTGCGcc < 1:1128346/69‑1 (MQ=255) tttGATCGCCATGATGACTTCTTCAAGGGAACAGTTACCGGCACGCTCGCCGATCCCGTTCATTGCGcc < 1:1287469/69‑1 (MQ=255) tttGATCGCCATGATGACTTCTTCAAGGGAACAGTTACCGGCACGCTCGCCGATCCCGTTCATTGCGcc < 1:2065017/69‑1 (MQ=255) tttGATCGCCATGATGACTTCTTCAAGGGAACAGTTACCGGCACGCTCGCCGATCCCGTTCATTGCGcc < 1:2175254/69‑1 (MQ=255) tttGATCGCCATGATGACTTCTTCAAGGGAACAGTTACCGGCACGCTCGCCGATCCCGTTCATTGCGcc < 1:2317967/69‑1 (MQ=255) tttGATCGCCATGATGACTTCTTCAAGGGAACAGTTACCGGCACGCTCGCCGATCCCGTTCATTGCGcc < 1:255200/69‑1 (MQ=255) tttGATCGCCATGATGACTTCTTCAAGGGAACAGTTACCGGCACGCTCGCCGATCCCGTTCATTGCGcc < 1:262866/69‑1 (MQ=255) tttGATCGCCATGATGACTTCTTCAAGGGAACAGTTACCGGCACGCTCGCCGATCCCGTTCATTGCGcc < 1:2721420/69‑1 (MQ=255) tttGATCGCCATGATGACTTCTTCAAGGGAACAGTTACCGGCACGCTCGCCGATCCCGTTCATTGCGcc < 1:3167728/69‑1 (MQ=255) tGATCGCCATGATGACTTCTTCCAGGGAACAGTTTCCGGCACGCTCGCCGATCCCGTTCATTGCGCCt > 1:2304658/1‑68 (MQ=255) aTCGCCATGATGACTTCTTCCAGGGAACAGTTTCCGGCACGCTCGCCGATCCCGTTCATTGCGCCTTc < 1:739951/68‑1 (MQ=255) aTCGCCATGATGACTTCTTCCAGGGAACAGTTTCCGGCACGCTCGCCGATCCCGTTCATTGCGCCTTc < 1:895818/68‑1 (MQ=255) aTCGCCATGATGACTTCTTCCAGGGAACAGTTTCCGGCACGCTCGCCGATCCCGTTCATTGCGCCTTc < 1:1219043/68‑1 (MQ=255) cGCCATGATGACTTCTTCCAGGGAACAGTTTCCGGCACGCTCGCCGATCCCGTTCATTGCGCCTTCCa < 1:1689084/68‑1 (MQ=255) cGCCATGATGACTTCTTCCAGGGAACAGTTTCCGGCACGCTCGCCGATCCCGTTCATTGCGCCTTCCa < 1:963073/68‑1 (MQ=255) tgatgaCTTCTTCCAGTGAACAGTTTCCGGCACGCTCGCCGa < 1:1517413/42‑1 (MQ=255) gatgaCTTCTTCCAGGGAACAGTTTCCGGCACGCTCGCCGATCCCGTTCATTGCGCCTTCCACCTGGCg < 1:613282/69‑1 (MQ=255) atgaCTTCTTCCAGGGAACAGTTTCCTGCACGCTCGCCGATCCCGTTCATTGCGCCTTcc > 1:2487183/1‑60 (MQ=255) atgaCTTCTTCCAGGGAACAGTTTCCGGCACGCTCGCCGATCCCGTTCATTGCGCCTTCCACCTGGCg > 1:2767027/1‑68 (MQ=255) atgaCTTCTTCCAGGGAACAGTTTCCGGCACGCTCGCCGATCCCGTTCATTGCGCCTTCCACCTGGCg > 1:2908553/1‑68 (MQ=255) atgaCTTCTTCCAGGGAACAGTTTCCGGCACGCTCGCCGATCCCGTTCATTGCGCCTTCCACCTGGCg > 1:3410340/1‑68 (MQ=255) gaCTTCTTCCAGGGAACAGTTTCCGGCACGCTCGCCGATCCCGTTCATTGCGCCTTCCACCTGGCGTGc > 1:2957797/1‑69 (MQ=255) | TGATTAATGGCGGTGTGGACGTTGAGAATATCCTTACGAACTTTGATCGCCATGATGACTTCTTCCAGGGAACAGTTTCCGGCACGCTCGCCGATCCCGTTCATTGCGCCTTCCACCTGGCGTGC > minE/67482‑67606 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |