| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 615,035 | T→C | 36.0% | Y228Y (TAT→TAC) | serC → | 3‑phosphoserine/phosphohydroxythreonine aminotransferase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 615,035 | 0 | T | C | 36.0% | 86.3 / 85.1 | 136 | Y228Y (TAT→TAC) | serC | 3‑phosphoserine/phosphohydroxythreonine aminotransferase |
| Reads supporting (aligned to +/- strand): ref base T (38/49); new base C (28/21); total (66/70) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.55e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.82e-01 | |||||||||||
Only 100 of 144 total aligned reads displayed.
ACAATCGTCATCGTTCGTGAAGATTTGCTGGGCAAAGCGAATATCGCGTGTCCGTCGATTCTGGATTATTCCATCCTCAACGATAACGGCTCCATGTTTAACACGCCGCCGACATTTGCCTGGTATCT > minE/614967‑615094 | aCAATCGTCATCGTTCGTGAAGATTTGCTGGGCAAAGCGAATATCGCGTGTCCGTCGATTCTGGattat > 1:1827932/1‑69 (MQ=255)aCAATCGTCATCGTTCGTGAAGATTTGCTGGGCAAAGCGAATATCGCGTGTCCGTCGATTCTGGattat > 1:2652800/1‑69 (MQ=255)aCAATCGTCATCGTTCGTGAAGATTTGCTGGGCAAAGCGAATATCGCGTGTCCGTCGATTCTGGattat > 1:685623/1‑69 (MQ=255)aCAATCGTCATCGTTCGTGAAGATTTGCTGGGCAAAGCGAATATCGCGTGTCCGTCGATTCTGGattat > 1:1525293/1‑69 (MQ=255)aCAATCGTCATCGTTCGTGAAGATTTGCTGGGCAAAGCGAATATCGCGTGTCCGTCGATTCTGGattat > 1:569072/1‑69 (MQ=255)aCAATCGTCATCGTTCGTGAAGATTTGCTGGGCAAAGCGAATATCGCGTGTCCGTCGATTCTGGattat > 1:1389095/1‑69 (MQ=255)aCAATCGTCATCGTTCGTGAAGATTTGCTGGGCAAAGCGAATATCGCGTGTCCGTCGATTCTGGattat > 1:2370886/1‑69 (MQ=255) cGTCATCGTTCGTGAAGATTTGCTGGGCAAAGCGAATATCGCGTGTCCGTCGATTCTGGATTATTcc > 1:2329379/1‑67 (MQ=255) cGTCATCGTTCGTGAAGATTTGCTGGGCAAAGCGAATATCGCGTGTCCGTCGATTCTGGATTATTcc > 1:348931/1‑67 (MQ=255) cGTCATCGTTCGTGAAGATTTGCTGGGCAAAGCGAATATCGCGTGTCCGTCGATTCTGGATTATTcc > 1:3350628/1‑67 (MQ=255) gTCATCGTTCGTGAAGATTTGCTGGGCAAAGCGAATATCGCGTGTCCGTCGATTCTGGATTATTc < 1:841809/65‑1 (MQ=255) gTCATCGTTCGTGAAGATTTGCTGGGCAAAGCGAATATCGCGTGTCCGTCGATTCTGGATTATTc < 1:1191846/65‑1 (MQ=255) gTCATCGTTCGTGAAGATTTGCTGGGCAAAGCGAATATCGCGTGTCCGTCGATTCTGGATTATTc < 1:119007/65‑1 (MQ=255) gTCATCGTTCGTGAAGATTTGCTGGGCAAAGCGAATATCGCGTGTCCGTCGATTCTGGATTATTc < 1:2130678/65‑1 (MQ=255) gTCATCGTTCGTGAAGATTTGCTGGGCAAAGCGAATATCGCGTGTCCGTCGATTCTGGATTATTc < 1:545217/65‑1 (MQ=255) gTCATCGTTCGTGAAGATTTGCTGGGCAAAGCGAATATCGCGTGTCCGTCGATTCTGGATTATTc < 1:40449/65‑1 (MQ=255) gTCATCGTTCGTGAAGATTTGCTGGGCAAAGCGAATATCGCGTGTCCGTCGATTCTGGATTATTc < 1:1813588/65‑1 (MQ=255) gTCATCGTTCGTGAAGATTTGCTGGGCAAAGCGAATATCGCGTGTCCGTCGATTCTGGATTATTc < 1:2921287/65‑1 (MQ=255) gTCATCGTTCGTGAAGATTTGCTGGGCAAAGCGAATATCGCGTGTCCGTCGATTCTGGATTATTCCAt > 1:2131587/1‑68 (MQ=255) gTCATCGTTCGTGAAGATTTGCTGGGCAAAGCGAATATCGCGTGTCCGTCGATTCTGGATTATTCCATc > 1:2402844/1‑69 (MQ=255) gTCATCGTTCGTGAAGATTTGCTGGGCAAAGCGAATATCGCGTGTCCGTCGATTCTGGATTATTCCATc > 1:2018112/1‑69 (MQ=255) gTCATCGTTCGTGAAGATTTGCTGGGCAAAGCGAATATCGCGTGTCCGTCGATTCTGGATTATTCCATc > 1:587677/1‑69 (MQ=255) gTCATCGTTCGTGAAGATTTGCTGGGCAAAGCGAATATCGCGTGTCCGTCGATTCTGGATTATTCCATc > 1:2957107/1‑69 (MQ=255) gTCATCGTTCGTGAAGATTTGCTGGGCAAAGCGAATATCGCGTGTCCGTCGATTCTGGATTATTCCATc > 1:1955525/1‑69 (MQ=255) gTCATCGTTCGTGAAGATTTGCTGGGCAAAGCGAATATCGCGTGTCCGTCGATTCTGGATTATTCCATc > 1:865445/1‑69 (MQ=255) gTCATCGTTCGTGAAGATTTGCTGGGCAAAGCGAATATCGCGTGTCCGTCGATTCTGGATTATTCCATc > 1:1771004/1‑69 (MQ=255) gTCATCGTTCGTGAAGATTTGCTGGGCAAAGCGAATATCGCGTGTCCGTCGATTCTGGATTATTCCATc > 1:1868318/1‑69 (MQ=255) tCATCGTTCGTGAAGATTTGCTGGGCAAAGCGAATATCGCGTGTCCGTCGATTCTGGATTATTc < 1:2758935/64‑1 (MQ=255) tGCTGGGCAAAGCGAATATCGCGTGTCCGTCGATTCTGGATTATTCCATCCTCAACGATAACGGCTCCa > 1:1016786/1‑69 (MQ=255) tGCTGGGCAAAGCGAATATCGCGTGTCCGTCGATTCTGGATTATTCCATCCTCAACGATAACGGCTCCa > 1:2320464/1‑69 (MQ=255) tGGGCAAAGCGAATATCGCGTGTCCGTCGATTCTGGATTATTCCATCCTCAACGATAACGGCTCCATg < 1:2495653/68‑1 (MQ=255) tGGGCAAAGCGAATATCGCGTGTCCGTCGATTCTGGATTATTCCATCCTCAACGATAACGGCTCCATg < 1:2012771/68‑1 (MQ=255) tGGGCAAAGCGAATATCGCGTGTCCGTCGATTCTGGATTATTCCATCCTCAACGATAACGGCTCCATg < 1:1403376/68‑1 (MQ=255) tGGGCAAAGCGAATATCGCGTGTCCGTCGATTCTGGATTATTCCATCCTCAACGATAACGGCTCCATg < 1:1704575/68‑1 (MQ=255) gCAAAGCGAATATCGCGTGTCCGTCGATTCTGGATTATTc < 1:385919/40‑1 (MQ=255) cAAAGCGAATATCGCGTGTCCGTCGATTCTGGATTATTCCATCCTCAACGATAACGGCTCCATGttt < 1:2266509/67‑1 (MQ=255) cAAAGCGAATATCGCGTGTCCGTCGATTCTGGATTATTCCATCCTCAACGATAACGGCTCCATGttt < 1:3315192/67‑1 (MQ=255) cAAAGCGAATATCGCGTGTCCGTCGATTCTGGATTATTCCATCCTCAACGATAACGGCTCCATGttt < 1:1436857/67‑1 (MQ=255) cAAAGCGAATATCGCGTGTCCGTCGATTCTGGATTATTCCATCCTCAACGATAACGGCTCCATGttt < 1:2136304/67‑1 (MQ=255) cAAAGCGAATATCGCGTGTCCGTCGATTCTGGATTATTCCATCCTCAACGATAACGGCTCCATGttt < 1:1662265/67‑1 (MQ=255) cAAAGCGAATATCGCGTGTCCGTCGATTCTGGATTATTCCATCCTCAACGATAACGGCTCCATGTTTaa < 1:595993/69‑1 (MQ=255) cAAAGCGAATATCGCGTGTCCGTCGATTCTGGATTATTCCATCCTCAACGATAACGGCTCCATGTTTaa < 1:3074302/69‑1 (MQ=255) cAAAGCGAATATCGCGTGTCCGTCGATTCTGGATTATTCCATCCTCAACGATAACGGCTCCATGTTTaa < 1:2531606/69‑1 (MQ=255) cAAAGCGAATATCGCGTGTCCGTCGATTCTGGATTATTCCATCCTCAACGATAACGGCTCCATGTTTaa < 1:77056/69‑1 (MQ=255) cAAAGCGAATATCGCGTGTCCGTCGATTCTGGATTATTCCATCCTCAACGATAACGGCTCCATGTTTaa < 1:1128620/69‑1 (MQ=255) cAAAGCGAATATCGCGTGTCCGTCGATTCTGGATTATTCCATCCTCAACGATAACGGCTCCATGTTTaa < 1:599385/69‑1 (MQ=255) aaaGCGAATATCGCCTGCCCATCGATTCTGGATTACTCCATCCTCAACGATAACGATTCCAt < 1:1738739/62‑1 (MQ=255) aaaGCGAATATCGCCTGCCCATCGATTCTGGATTACTCCATCCTCAACGATAACGATTCCAt < 1:698796/62‑1 (MQ=255) aaaGCGAATATCGCCTGCCCATCGATTCTGGATTACTCCATCCTCAACGATAACGATTCCAt < 1:1929735/62‑1 (MQ=255) aaaGCGAATATCGCCTGCCCATCGATTCTGGATTACTCCATCCTCAACGATAACGATTCCAt < 1:2770392/62‑1 (MQ=255) aaaGCGAATATCGCCTGCCCATCGATTCTGGATTACTCCATCCTCAACGATAACGATTCCAt < 1:1804135/62‑1 (MQ=255) aaaGCGAATATCGCCTGCCCATCGATTCTGGATTACTCCATCCTCAACGATAACGATTCCAt < 1:2251158/62‑1 (MQ=255) aaaGCGAATATCGCCTGCCCATCGATTCTGGATTACTCCATCCTCAACGATAACGATTCCAt < 1:1531487/62‑1 (MQ=255) aaaGCGAATATCGCCTGCCCATCGATTCTGGATTACTCCATCCTCAACGATAACGATTCCAt < 1:434736/62‑1 (MQ=255) aaaGCGAATATCGCCTGCCCATCGATTCTGGATTACTCCATCCTCAACGATAACGATTCCAt < 1:2191608/62‑1 (MQ=255) aaaGCGAATATCGCCTGCCCATCGATTCTGGATTACTCCATCCTCAACAATAACGATTCCAt < 1:2996315/62‑1 (MQ=255) aaGCGAATATCGCCTGCCCATCGATTCTGGATTACTCCATCCTCAACGATAACGATTCCAt < 1:548238/61‑1 (MQ=255) aGCGAATATCGCGTGTCCGTCGATTCTGGATTATTCCATCCTCAACGATAACGGCTCCATGTTTAacac < 1:272812/69‑1 (MQ=255) aGCGAATATCGCGTGTCCGTCGATTCTGGATTATTCCATCCTCAACGATAACGGCTCCATGTTTAacac < 1:863835/69‑1 (MQ=255) gcgTGTCCGTCGATTCTGGATTATTCCATCCTCAACGATAACGGCTCCATGTTTAACACGCCGCCGCCa < 1:1461563/69‑1 (MQ=255) gcgTGTCCGTCGATTCTGGATTATTCCATCCTCAACGATAACGGCTCCATGTTTAACACGCCGCCGACa < 1:2974625/69‑1 (MQ=255) gcgTGTCCGTCGATTCTGGATTATTCCATCCTCAACGATAACGGCTCCATGTTTAACACGCCGCCGACa < 1:3186298/69‑1 (MQ=255) gcgTGTCCGTCGATTCTGGATTATTCCATCCTCAACGATAACGGCTCCATGTTTAACACGCCGCCGACa < 1:615599/69‑1 (MQ=255) gcgTGTCCGTCGATTCTGGATTATTCCATCCTCAACGATAACGGCTCCATGTTTAACACGCCGCCGACa < 1:1992178/69‑1 (MQ=255) gcgTGTCCGTCGATTCTGGATTATTCCATCCTCAACGATAACGGCTCCATGTTTAACACGCCGCCGACa < 1:1901405/69‑1 (MQ=255) gcgTGTCCGTCGATTCTGGATTATTCCATCCTCAACGATAACGGCTCCATGTTTAACACGCCGCCGACa < 1:975698/69‑1 (MQ=255) ccGTCGATTCTGGATTATTCCATCCTCAACGATAACGGCTCCATGTTTAACACGCCGCCGACATTTGcc > 1:755896/1‑69 (MQ=255) ccGTCGATTCTGGATTATTCCATCCTCAACGATAACGGCTCCATGTTTAACACGCCGCCGACATTTGcc > 1:3381704/1‑69 (MQ=255) ccGTCGATTCTGGATTATTCCATCCTCAACGATAACGGCTCCATGTTTAACACGCCGCCGACATTTGcc > 1:1552367/1‑69 (MQ=255) cGTCGATTCTGGATTATTCCATCCTCAACGATAACGGCTCCATGTTTAACACGCCGCCGACATTTGCCt < 1:1057227/69‑1 (MQ=255) gATTCTGGTTTACTCCATCCTCAACGATAACGATTCCAt < 1:2472758/39‑1 (MQ=25) gATTCTGGATTACTCCATCCTCAACGATAACGATTCCATGTTCAac > 1:316965/1‑46 (MQ=255) gATTCTGGATTACTCCATCCTCAACGATAACGATTCCATGTTCAac > 1:967627/1‑46 (MQ=255) gATTCTGGATTACTCCATCCTCAACGATAACGATTCCATGTTCAac > 1:1088381/1‑46 (MQ=255) gATTCTGGATTACTCCATCCTCAACGATAACGATTCCATGTTCAac > 1:1269048/1‑46 (MQ=255) gATTCTGGATTACTCCATCCTCAACGATAACGATTCCATGTTCAac > 1:1389405/1‑46 (MQ=255) gATTCTGGATTACTCCATCCTCAACGATAACGATTCCATGTTCAac > 1:1776450/1‑46 (MQ=255) gATTCTGGATTACTCCATCCTCAACGATAACGATTCCATGTTCAac > 1:71841/1‑46 (MQ=255) gATTCTGGATTACTCCATCCTCAACGATAACGATTCCATGTTCAac > 1:693954/1‑46 (MQ=255) gATTCTGGATTACTCCATCCTCAACGATAACGATTCCATGTTCAac > 1:2082691/1‑46 (MQ=255) gATTCTGGATTACTCCATCCTCAACGATAACGATTCCATGTTCAac > 1:2159136/1‑46 (MQ=255) gATTCTGGATTACTCCATCCTCAACGATAACGATTCCATGTTCAac > 1:217016/1‑46 (MQ=255) gATTCTGGATTACTCCATCCTCAACGATAACGATTCCATGTTCAac > 1:2202095/1‑46 (MQ=255) gATTCTGGATTACTCCATCCTCAACGATAACGATTCCATGTTCAac > 1:2412733/1‑46 (MQ=255) gATTCTGGATTACTCCATCCTCAACGATAACGATTCCATGTTCAac > 1:3022708/1‑46 (MQ=255) gATTCTGGATTACTCCATCCTCAACGATAACGATTCCATGTTCAac > 1:2962818/1‑46 (MQ=255) gATTCTGGATTACTCCATCCTCAACGATAACGATTCCATGTTCAac > 1:2812168/1‑46 (MQ=255) gATTCTGGATTACTCCATCCTCAACGATAACGATTCCATGTTCAac > 1:2638824/1‑46 (MQ=255) gATTCTGGATTACTCCATCCTCAACGATAACGATTCCATGTTCAac > 1:2530137/1‑46 (MQ=255) gATTCTGGATTACTCCATCCTCAACGATAACGATTCCATGTTCAac > 1:2483330/1‑46 (MQ=255) gATTCTGGATTACTCCATCCTCAACGATAACGATTCCATGTTCAac > 1:2351231/1‑46 (MQ=255) tCTGGATTATTCCATCCTCAACGATAACGGCTCCATGTTTAACACGCCGCCGACATTTGCCTGGtatct < 1:2066864/69‑1 (MQ=255) cTGGATTATTCCATCCTCAACGATAACGGCTCCATGTTTAACACGCCGCCGAc > 1:642930/1‑53 (MQ=255) cTGGATTATTCCATCCTCAACGATAACGGCTCCATGTTTAACACGCCGCCGAc > 1:1986862/1‑53 (MQ=255) cTGGATTATTCCATCCTCAACGATAACGGCTCCATGTTTAACACGCCGCCGAc > 1:757916/1‑53 (MQ=255) cTGGATTATTCCATCCTCAACGATAACGGCTCCATGTTTAACACGCCGCCGAc > 1:47696/1‑53 (MQ=255) cTGGATTATTCCATCCTCAACGATAACGGCTCCATGTTTAACACGCCGCCGAc > 1:3064436/1‑53 (MQ=255) cTGGATTATTCCATCCTCAACGATAACGGCTCCATGTTTAACACGCCGCCGAc > 1:1032027/1‑53 (MQ=255) cTGGATTATTCCATCCTCAACGATAACGGCTCAATGTTTAACACGCCGCCGAc > 1:3284511/1‑53 (MQ=255) ggATTATTCCATCCTCAACGATAACGTCTCCATGttt < 1:2047288/37‑1 (MQ=255) | ACAATCGTCATCGTTCGTGAAGATTTGCTGGGCAAAGCGAATATCGCGTGTCCGTCGATTCTGGATTATTCCATCCTCAACGATAACGGCTCCATGTTTAACACGCCGCCGACATTTGCCTGGTATCT > minE/614967‑615094 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |