| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 615,055 | G→A | 45.8% | G235D (GGC→GAC) ‡ | serC → | 3‑phosphoserine/phosphohydroxythreonine aminotransferase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 615,055 | 0 | G | A | 45.8% | 24.7 / 101.3 | 123 | G235D (GGC→GAC) ‡ | serC | 3‑phosphoserine/phosphohydroxythreonine aminotransferase |
| Reads supporting (aligned to +/- strand): ref base G (30/36); new base A (28/28); total (58/65) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 7.16e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.77e-01 | |||||||||||
Only 100 of 123 total aligned reads displayed.
TGCTGGGCAAAGCGAATATCGCGTGTCCGTCGATTCTGGATTATTCCATCCTCAACGATAACGGCTCCATGTTTAACACGCCGCCGACATTTGCCTGGTATCTATCTGGTCTGGT > minE/614992‑615106 | tGCTGGGCAAAGCGAATATCGCGTGTCCGTCGATTCTGGATTATTCCATCCTCAACGATAACGGCTCCa > 1:1826383/1‑69 (MQ=255)tGCTGGGCAAAGCGAATATCGCGTGTCCGTCGATTCTGGATTATTCCATCCTCAACGATAACGGCTCCa > 1:1016786/1‑69 (MQ=255)tGCTGGGCAAAGCGAATATCGCGTGTCCGTCGATTCTGGATTATTCCATCCTCAACGATAACGGCTCCa > 1:2320464/1‑69 (MQ=255)tGCTGGGCAAAGCGAATATCGCGTGTCCGTCGATTCTGGATTATTCCATCCTCAACGATAACGGCTCCa > 1:2973246/1‑69 (MQ=255) tGGGCAAAGCGAATATCGCGTGTCCGTCGATTCTGGATTATTCCATCCTCAACGATAACGGCTCCATg < 1:2495653/68‑1 (MQ=255) tGGGCAAAGCGAATATCGCGTGTCCGTCGATTCTGGATTATTCCATCCTCAACGATAACGGCTCCATg < 1:2012771/68‑1 (MQ=255) tGGGCAAAGCGAATATCGCGTGTCCGTCGATTCTGGATTATTCCATCCTCAACGATAACGGCTCCATg < 1:1704575/68‑1 (MQ=255) cAAAGCGAATATCGCGTGTCCGTCGATTCTGGATTATTCCATCCTCAACGATAACGGCTCCATGttt < 1:1436857/67‑1 (MQ=255) cAAAGCGAATATCGCGTGTCCGTCGATTCTGGATTATTCCATCCTCAACGATAACGGCTCCATGttt < 1:2266509/67‑1 (MQ=255) cAAAGCGAATATCGCGTGTCCGTCGATTCTGGATTATTCCATCCTCAACGATAACGGCTCCATGttt < 1:1662265/67‑1 (MQ=255) cAAAGCGAATATCGCGTGTCCGTCGATTCTGGATTATTCCATCCTCAACGATAACGGCTCCATGttt < 1:3315192/67‑1 (MQ=255) cAAAGCGAATATCGCGTGTCCGTCGATTCTGGATTATTCCATCCTCAACGATAACGGCTCCATGTTTaa < 1:2531606/69‑1 (MQ=255) cAAAGCGAATATCGCGTGTCCGTCGATTCTGGATTATTCCATCCTCAACGATAACGGCTCCATGTTTaa < 1:3391691/69‑1 (MQ=255) cAAAGCGAATATCGCGTGTCCGTCGATTCTGGATTATTCCATCCTCAACGATAACGGCTCCATGTTTaa < 1:595993/69‑1 (MQ=255) cAAAGCGAATATCGCGTGTCCGTCGATTCTGGATTATTCCATCCTCAACGATAACGGCTCCATGTTTaa < 1:599385/69‑1 (MQ=255) cAAAGCGAATATCGCGTGTCCGTCGATTCTGGATTATTCCATCCTCAACGATAACGGCTCCATGTTTaa < 1:3224386/69‑1 (MQ=255) cAAAGCGAATATCGCGTGTCCGTCGATTCTGGATTATTCCATCCTCAACGATAACGGCTCCATGTTTaa < 1:77056/69‑1 (MQ=255) cAAAGCGAATATCGCGTGTCCGTCGATTCTGGATTATTCCATCCTCAACGATAACGGCTCCATGTTTaa < 1:1128620/69‑1 (MQ=255) aaaGCGAATATCGCCTGCCCATCGATTCTGGATTACTCCATCCTCAACGATAACGATTCCAt < 1:2128909/62‑1 (MQ=255) aaaGCGAATATCGCCTGCCCATCGATTCTGGATTACTCCATCCTCAACGATAACGATTCCAt < 1:2191608/62‑1 (MQ=255) aaaGCGAATATCGCCTGCCCATCGATTCTGGATTACTCCATCCTCAACGATAACGATTCCAt < 1:2770392/62‑1 (MQ=255) aaaGCGAATATCGCCTGCCCATCGATTCTGGATTACTCCATCCTCAACGATAACGATTCCAt < 1:2042774/62‑1 (MQ=255) aaaGCGAATATCGCCTGCCCATCGATTCTGGATTACTCCATCCTCAACGATAACGATTCCAt < 1:2283802/62‑1 (MQ=255) aaaGCGAATATCGCCTGCCCATCGATTCTGGATTACTCCATCCTCAACGATAACGATTCCAt < 1:272270/62‑1 (MQ=255) aaaGCGAATATCGCCTGCCCATCGATTCTGGATTACTCCATCCTCAACGATAACGATTCCAt < 1:1929735/62‑1 (MQ=255) aaaGCGAATATCGCCTGCCCATCGATTCTGGATTACTCCATCCTCAACGATAACGATTCCAt < 1:434736/62‑1 (MQ=255) aaaGCGAATATCGCCTGCCCATCGATTCTGGATTACTCCATCCTCAACGATAACGATTCCAt < 1:621242/62‑1 (MQ=255) aaaGCGAATATCGCCTGCCCATCGATTCTGGATTACTCCATCCTCAACGATAACGATTCCAt < 1:698796/62‑1 (MQ=255) aaaGCGAATATCGCCTGCCCATCGATTCTGGATTACTCCATCCTCAACGATAACGATTCCAt < 1:157780/62‑1 (MQ=255) aaaGCGAATATCGCCTGCCCATCGATTCTGGATTACTCCATCCTCAACGATAACGATTCCAt < 1:1738739/62‑1 (MQ=255) aaaGCGAATATCGCCTGCCCATCGATTCTGGATTACTCCATCCTCAACGATAACGATTCCAt < 1:1804135/62‑1 (MQ=255) aaaGCGAATATCGCCTGCCCATCGATTCTGGATTACTCCATCCTCAACAATAACGATTCCAt < 1:2996315/62‑1 (MQ=255) aaGCGAATATCGCCTGCCCATCGATTCTGGATTACTCCATCCTCAACGATAACGATTCCAt < 1:548238/61‑1 (MQ=255) aGCGAATATCGCGTGTCCGTCGATTCTGGATTATTCCATCCTCAACGATAACGGCTCCATGTTTAacac < 1:863835/69‑1 (MQ=255) aGCGAATATCGCGTGTCCGTCGATTCTGGATTATTCCATCCTCAACGATAACGGCTCCATGTTTAacac < 1:272812/69‑1 (MQ=255) aaTATCGGCTGGCCATCGATTCTGGATTACTCCATCCTCAACGATAACGATTCCAt < 1:2132655/56‑1 (MQ=255) tCGCGTGTCCGTCGATTCTGGATTATTCCATCCTCAACGATAACGGCTCCATGTTTAACACGCAGCCGa > 1:1963873/1‑69 (MQ=255) gcgTGTCCGTCGATTCTGGATTATTCCATCCTCAACGATAACGGCTCCATGTTTAACACGCCGCCGCCa < 1:1461563/69‑1 (MQ=255) gcgTGTCCGTCGATTCTGGATTATTCCATCCTCAACGATAACGGCTCCATGTTTAACACGCCGCCGACa < 1:261932/69‑1 (MQ=255) gcgTGTCCGTCGATTCTGGATTATTCCATCCTCAACGATAACGGCTCCATGTTTAACACGCCGCCGACa < 1:1227620/69‑1 (MQ=255) gcgTGTCCGTCGATTCTGGATTATTCCATCCTCAACGATAACGGCTCCATGTTTAACACGCCGCCGACa < 1:975698/69‑1 (MQ=255) gcgTGTCCGTCGATTCTGGATTATTCCATCCTCAACGATAACGGCTCCATGTTTAACACGCCGCCGACa < 1:69972/69‑1 (MQ=255) gcgTGTCCGTCGATTCTGGATTATTCCATCCTCAACGATAACGGCTCCATGTTTAACACGCCGCCGACa < 1:145142/69‑1 (MQ=255) gcgTGTCCGTCGATTCTGGATTATTCCATCCTCAACGATAACGGCTCCATGTTTAACACGCCGCCGACa < 1:153080/69‑1 (MQ=255) gcgTGTCCGTCGATTCTGGATTATTCCATCCTCAACGATAACGGCTCCATGTTTAACACGCCGCCGACa < 1:3186298/69‑1 (MQ=255) gcgTGTCCGTCGATTCTGGATTATTCCATCCTCAACGATAACGGCTCCATGTTTAACACGCCGCCGACa < 1:1992178/69‑1 (MQ=255) ccGTCGATTCTGGATTATTCCATCCTCAACGATAACGGCTCCATGTTTAACACGCCGCCGACATTTGcc > 1:1552367/1‑69 (MQ=255) ccGTCGATTCTGGATTATTCCATCCTCAACGATAACGGCTCCATGTTTAACACGCCGCCGACATTTGcc > 1:3077426/1‑69 (MQ=255) ccGTCGATTCTGGATTATTCCATCCTCAACGATAACGGCTCCATGTTTAACACGCCGCCGACATTTGcc > 1:755896/1‑69 (MQ=255) ccGTCGATTCTGGATTATTCCATCCTCAACGATAACGGCTCCATGTTTAACACGCCGCCGACATTTGcc > 1:3381704/1‑69 (MQ=255) ccGTCGATTCTGGATTATTCCATCCTCAACGATAACGGCTCCATGTTTAACACGCCGCCGACATTTGcc > 1:2443991/1‑69 (MQ=255) ccGTCGATTCTGGATTATTCCATCCTCAACGATAACGGCTCCATGTTTAACACGCCGCCGACATTTGcc > 1:1802825/1‑69 (MQ=255) cGTCGATTCTGGATTATTCCATCCTCAACGATAACGGCTCCATGTTTAACACGCCGCCGACATTTGCCt < 1:1057227/69‑1 (MQ=255) gATTCTGGTTTACTCCATCCTCAACGATAACGATTCCAt < 1:2472758/39‑1 (MQ=25) gATTCTGGATTACTCCATCCTTAACGATAACGATTCCATGTTCAac > 1:2997454/1‑46 (MQ=25) gATTCTGGATTACTCCATCCTCAACGATAACGATTCCATGTTCaa > 1:761690/1‑45 (MQ=255) gATTCTGGATTACTCCATCCTCAACGATAACGATTCCATGTTCAac > 1:1088381/1‑46 (MQ=255) gATTCTGGATTACTCCATCCTCAACGATAACGATTCCATGTTCAac > 1:1776450/1‑46 (MQ=255) gATTCTGGATTACTCCATCCTCAACGATAACGATTCCATGTTCAac > 1:597674/1‑46 (MQ=255) gATTCTGGATTACTCCATCCTCAACGATAACGATTCCATGTTCAac > 1:1749551/1‑46 (MQ=255) gATTCTGGATTACTCCATCCTCAACGATAACGATTCCATGTTCAac > 1:1890171/1‑46 (MQ=255) gATTCTGGATTACTCCATCCTCAACGATAACGATTCCATGTTCAac > 1:693954/1‑46 (MQ=255) gATTCTGGATTACTCCATCCTCAACGATAACGATTCCATGTTCAac > 1:1399831/1‑46 (MQ=255) gATTCTGGATTACTCCATCCTCAACGATAACGATTCCATGTTCAac > 1:1389405/1‑46 (MQ=255) gATTCTGGATTACTCCATCCTCAACGATAACGATTCCATGTTCAac > 1:2082691/1‑46 (MQ=255) gATTCTGGATTACTCCATCCTCAACGATAACGATTCCATGTTCAac > 1:316965/1‑46 (MQ=255) gATTCTGGATTACTCCATCCTCAACGATAACGATTCCATGTTCAac > 1:1269048/1‑46 (MQ=255) gATTCTGGATTACTCCATCCTCAACGATAACGATTCCATGTTCAac > 1:3022708/1‑46 (MQ=255) gATTCTGGATTACTCCATCCTCAACGATAACGATTCCATGTTCAac > 1:2159136/1‑46 (MQ=255) gATTCTGGATTACTCCATCCTCAACGATAACGATTCCATGTTCAac > 1:217016/1‑46 (MQ=255) gATTCTGGATTACTCCATCCTCAACGATAACGATTCCATGTTCAac > 1:2962818/1‑46 (MQ=255) gATTCTGGATTACTCCATCCTCAACGATAACGATTCCATGTTCAac > 1:953750/1‑46 (MQ=255) gATTCTGGATTACTCCATCCTCAACGATAACGATTCCATGTTCAac > 1:2926321/1‑46 (MQ=255) gATTCTGGATTACTCCATCCTCAACGATAACGATTCCATGTTCAac > 1:2812168/1‑46 (MQ=255) gATTCTGGATTACTCCATCCTCAACGATAACGATTCCATGTTCAac > 1:2202095/1‑46 (MQ=255) gATTCTGGATTACTCCATCCTCAACGATAACGATTCCATGTTCAac > 1:2351231/1‑46 (MQ=255) gATTCTGGATTACTCCATCCTCAACGATAACGATTCCATGTTCAac > 1:2412733/1‑46 (MQ=255) gATTCTGGATTACTCCATCCTCAACGATAACGATTCCATGTTCAac > 1:967627/1‑46 (MQ=255) gATTCTGGATTACTCCATCCTCAACGATAACGATTCCATGTTCAac > 1:2483330/1‑46 (MQ=255) gATTCTGGATTACTCCATCCTCAACGATAACGATTCCATGTTCAac > 1:2638824/1‑46 (MQ=255) tCTGGATTATTCCATCCTCAACGATAACGGCTCCATGTTTAACACGCCGCCGACATTTGCCTGGtatct < 1:2066864/69‑1 (MQ=255) cTGGATTATTCCATCCTCAACGATAACGGCTCCATGTTTAACACGCCGCCGAc > 1:1032027/1‑53 (MQ=255) cTGGATTATTCCATCCTCAACGATAACGGCTCCATGTTTAACACGCCGCCGAc > 1:757916/1‑53 (MQ=255) cTGGATTATTCCATCCTCAACGATAACGGCTCCATGTTTAACACGCCGCCGAc > 1:642930/1‑53 (MQ=255) cTGGATTATTCCATCCTCAACGATAACGGCTCCATGTTTAACACGCCGCCGAc > 1:495457/1‑53 (MQ=255) cTGGATTATTCCATCCTCAACGATAACGGCTCCATGTTTAACACGCCGCCGAc > 1:47696/1‑53 (MQ=255) cTGGATTATTCCATCCTCAACGATAACGGCTCCATGTTTAACACGCCGCCGAc > 1:1986862/1‑53 (MQ=255) cTGGATTATTCCATCCTCAACGATAACGGCTCCATGTTTAACACGCCGCCGAc > 1:3064436/1‑53 (MQ=255) cTGGATTATTCCATCCTCAACGATAACGGCTCAATGTTTAACACGCCGCCGAc > 1:3284511/1‑53 (MQ=255) ggATTATTCCATCCTCAACGATAACGTCTCCATGttt < 1:2047288/37‑1 (MQ=255) ccATCCTCAACGATAACGGCTCCATGTTTAACACGCCGCCGACATTTGCCTGGTATCTAtctggtctgg > 1:1859209/1‑69 (MQ=255) ccATCCTCAACGATAACGGCTCCATGTTTAACACGCCGCCGACATTTGCCTGGTATCTAtctggtctgg > 1:1621876/1‑69 (MQ=255) ccATCCTCAACGATAACGGCTCCATGTTTAACACGCCGCCGACATTTGCCTGGTATCTAtctggtctgg > 1:1470328/1‑69 (MQ=255) ccATCCTCAACGATAACGGCTCCATGTTTAACACGCCGCCGACATTTGCCTGGTATCTAtctggtctgg > 1:3372302/1‑69 (MQ=255) ccATCCTCAACGATAACGGCTCCATGTTTAACACGCCGCCGACATTTGCCTGGTATCTAtctggtctgg > 1:2927475/1‑69 (MQ=255) tCCTCAACGATAACGATTCCATGTTCAACACGCCGCCGACATTTGCCTGGTACTTATctggcctggt < 1:601711/67‑1 (MQ=255) tCCTCAACGATAACGATTCCATGTTCAACACGCCGCCGACATTTGCCTGGTACTTATctggcctggt < 1:2080678/67‑1 (MQ=255) tCCTCAACGATAACGATTCCATGTTCAACACGCCGCCGACATTTGCCTGGTACTTATctggcctggt < 1:272242/67‑1 (MQ=255) tCCTCAACGATAACGATTCCATGTTCAACACGCCGCCGACATTTGCCTGGTACTTATctggcctggt < 1:2652213/67‑1 (MQ=255) ccTCAACGATAACGATTCCATGTTCAACACGCCGCCGACATTTGCCTGGTACTTATctggcctggt < 1:2123783/66‑1 (MQ=255) | TGCTGGGCAAAGCGAATATCGCGTGTCCGTCGATTCTGGATTATTCCATCCTCAACGATAACGGCTCCATGTTTAACACGCCGCCGACATTTGCCTGGTATCTATCTGGTCTGGT > minE/614992‑615106 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |