| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 67,673 | G→A | 39.2% | I199I (ATC→ATT) | leuA ← | 2‑isopropylmalate synthase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 67,673 | 0 | G | A | 39.2% | 40.1 / 51.2 | 79 | I199I (ATC→ATT) | leuA | 2‑isopropylmalate synthase |
| Reads supporting (aligned to +/- strand): ref base G (10/38); new base A (18/13); total (28/51) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.47e-03 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.97e-01 | |||||||||||
CCGGCATGTACCGCCGCCAGTGAGTTTCCGACCGCCAGGCCCAAATCGTCGTGGGTATGTACGGAGATAATGGCTTTGTCGATGTTAGGCACGCGTTCATACAGGCCGCTGATGATTCCGGCGAACTCAAACGG > minE/67608‑67741 | ccGGCATGTACCTCCGCCAGTGAGTTTCCGACCGCCAGGCCCAAATCGTCGTGGGTATGTACGGAGAt < 1:2529707/68‑1 (MQ=255)ccGGCATGTACCGCCGCCAGTGAGTTTCCGACCGCCAGGCCCAAATCGTCGTGGGTATGTACGGAGAt < 1:2669528/68‑1 (MQ=255)ccGGCATGTACCGCCGCCAGTGAGTTTCCGACCGCCAGGCCCAAATCGTCGTGGGTATGTACGGAGAt < 1:1833472/68‑1 (MQ=255)ccGGCATGTACCGCCGCCAGTGAGTTTCCGACCGCCAGGCCCAAATCGTCGTGGGTATGTACGGAGAt < 1:253081/68‑1 (MQ=255) aCCGCCGCCAGTGAGTTTCCGACCGCCAGGCCCAAATCGTCGTGGGTATGTACGGAGATAATGGCttt > 1:1718168/1‑68 (MQ=255) aCCGCCGCCAGTGAGTTTCCGACCGCCAGGCCCAAATCGTCGTGGGTATGTACGGAGATAATGGCTTTg > 1:1381416/1‑69 (MQ=255) gccgccAGTGAGTTTCCGACCGCCAGGCCCAAATCGTCGTGGGTATGTACGGAGATAATGGCTTTGTCg < 1:1699105/69‑1 (MQ=255) ccAGTGAGTTTCCGACCGCCAGGCCCAAATCGTCGTGGGTATGTACGGAGATAATGGCTTTGTCGATGt < 1:1015221/69‑1 (MQ=255) ccAGTGAGTTTCCGACCGCCAGGCCCAAATCGTCGTGGGTATGTACGGAGATAATGGCTTTGTCGATGt < 1:3142185/69‑1 (MQ=255) ccAGTGAGTTTCCGACCGCCAGGCCCAAATCGTCGTGGGTATGTACGGAGATAATGGCTTTGTCGATGt < 1:137285/69‑1 (MQ=255) ccAGTGAGTTTCCGACCGCCAGGCCCAAATCGTCGTGGGTATGTACGGAGATAATGGCTTTGTCGATGt < 1:303418/69‑1 (MQ=255) ccAGTGAGTTTCCGACCGCCAGGCCCAAATCGTCGTGGGTATGTACGGAGATAATGGCTTTGTCGATGt < 1:2732571/69‑1 (MQ=255) ccAGTGAGTTTCCGACCGCCAGGCCCAAATCGTCGTGGGTATGTACGGAGATAATGGCTTTGTCGATGt < 1:1885546/69‑1 (MQ=255) ccAGTGAGTTTCCGACCGCCAGGCCCAAATCGTCGTGGGTATGTACGGAGATAATGGCTTTGTCGATGt < 1:2360187/69‑1 (MQ=255) aGTGAGTTTCCGACCGCCAGGCCCAAATCGTCGTGGGTATGTACGGAGATAATGGCTTTGTCGATGt < 1:508867/67‑1 (MQ=255) aGTGAGTTGCCGACCGCCAGGCCCAAATCGTCGTGGGTATGTACGGAAAt < 1:2564201/50‑1 (MQ=255) aGTGAGTTGCCGACCGCCAGGCCCAAATCGTCGTGGGTATGTACGGAAAt < 1:1845691/50‑1 (MQ=255) aGTGAGTTGCCGACCGCCAGGCCCAAATCGTCGTGGGTATGTACGGAAAt < 1:2058131/50‑1 (MQ=255) aGTGAGTTGCCGACCGCCAGGCCCAAATCGTCGTGGGTATGTACGGAAAt < 1:2440299/50‑1 (MQ=255) aGTGAGTTGCCGACCGCCAGGCCCAAATCGTCGTGGGTATGTACGGAAAt < 1:510729/50‑1 (MQ=255) aGTGAGTTGCCGACCGCCAGGCCCAAATCGTCGTGGGTATGTACGGAAAt < 1:326565/50‑1 (MQ=255) aGTGAGTTGCCGACCGCCAGGCCCAAATCGTCGTGGGTATGTACGGAAAt < 1:2652634/50‑1 (MQ=255) aGTGAGTTGCCGACCGCCAGGCCCAAATCGTCGTGGGTATGTACGGAAAt < 1:997249/50‑1 (MQ=255) aGTGAGTTGCCGACCGCCAGGCCCAAATCGTCGTGGGTATGTACGGAAAt < 1:3098197/50‑1 (MQ=255) aGTGAGTTGCCGACCGCCAGGCCCAAATCGTCGTGGGTATGTACGGAAAt < 1:3261161/50‑1 (MQ=255) aGTGAGTTGCCGACCGCCAGGCCCAAATCGTCGTGGGTATGTACGGAAAt < 1:1816628/50‑1 (MQ=255) aGTGAGTTGCCGACCGCCAGGCCCAAATCGTCGTGGGTATGTACGGAAAt < 1:1445171/50‑1 (MQ=255) aGTGAGTTGCCGACCGCCAGGCCCAAATCGTCGTGGGTATGTACGGAAAt < 1:15429/50‑1 (MQ=255) tGAGTTTCCGACCGCCAGGCCCAAATCGTCGTGGGTATGTACGGAGAt < 1:1876641/48‑1 (MQ=255) tGAGTTTCCGACCGCCAGGCCCAAATCGTCGTGGGTATGTACGGAGATAATGGCTTTGTCGATGTTAgg < 1:1001280/69‑1 (MQ=255) tGAGTTTCCGACCGCCAGGCCCAAATCGTCGTGGGTATGTACGGAGATAATGGCTTTGTCGATGTTAgg < 1:1153859/69‑1 (MQ=255) tGAGTTTCCGACCGCCAGGCCCAAATCGTCGTGGGTATGTACGGAGATAATGGCTTTGTCGATGTTAgg < 1:2951574/69‑1 (MQ=255) tGAGTTTCCGACCGCCAGGCCCAAATCGTCGTGGGTATGTACGGAGATAATGGCTTTGTCGATGTTAgg < 1:2700013/69‑1 (MQ=255) tGAGTTTCCGACCGCCAGGCCCAAATCGTCGTGGGTATGTACGGAGATAATGGCTTTGTCGATGTTAgg < 1:1810484/69‑1 (MQ=255) tGAGTTTCCGACCGCCAGGCCCAAATCGTCGTGGGTATGTACGGAGATAATGGCTTTGTCGATGTTAgg < 1:1756095/69‑1 (MQ=255) aGTTTCCGACCGCCAGGCCCAAATCGTCGTGGGTATGTACGGAGATAATGGCTTTGTCGATGt < 1:2867524/63‑1 (MQ=255) ccAAATCGTCGTGGGTATGTACGGAAATAATTGCTTTGTCGATGTTGGGTACGCGTTCATACAGGCCg > 1:1290412/1‑68 (MQ=255) ccAAATCGTCGTGGGTATGTACGGAAATAATGGCTTTGTCGATGTTGGGTACGCGTTCAt > 1:150569/1‑60 (MQ=255) ccAAATCGTCGTGGGTATGTACGGAAATAATGGCTTTGTCGATGTTGGGTACGCGTTCATACAGGCCg > 1:1048628/1‑68 (MQ=255) ccAAATCGTCGTGGGTATGTACGGAAATAATGGCTTTGTCGATGTTGGGTACGCGTTCATACAGGCCg > 1:1080007/1‑68 (MQ=255) ccAAATCGTCGTGGGTATGTACGGAAATAATGGCTTTGTCGATGTTGGGTACGCGTTCATACAGGCCg > 1:591725/1‑68 (MQ=255) ccAAATCGTCGTGGGTATGTACGGAAATAATGGCTTTGTCGATGTTGGGTACGCGTTCATACAGGCCg > 1:503052/1‑68 (MQ=255) ccAAATCGTCGTGGGTATGTACGGAAATAATGGCTTTGTCGATGTTGGGTACGCGTTCATACAGGCCg > 1:1855736/1‑68 (MQ=255) ccAAATCGTCGTGGGTATGTACGGAAATAATGGCTTTGTCGATGTTGGGTACGCGTTCATACAGGCCg > 1:305895/1‑68 (MQ=255) ccAAATCGTCGTGGGTATGTACGGAAATAATGGCTTTGTCGATGTTGGGTACGCGTTCATACAGGCCg > 1:751922/1‑68 (MQ=255) ccAAATCGTCGTGGGTATGTACGGAAATAATGGCTTTGTCGATGTTGGGTACGCGTTCATACAGGCCg > 1:2038096/1‑68 (MQ=255) ccAAATCGTCGTGGGTATGTACGGAAATAATGGCTTTGTCGATGTTGGGTACGCGTTCATACAGGCCg > 1:82989/1‑68 (MQ=255) ccAAATCGTCGTGGGTATGTACGGAAATAATGGCTTTGTCGATGTTGGGTACGCGTTCATACAGGCCg > 1:2005381/1‑68 (MQ=255) ccAAATCGTCGTGGGTATGTACGGAAATAATGGCTTTGTCGATGTTGGGTACGCGTTCATACAGGCCg > 1:162305/1‑68 (MQ=255) ccAAATCGTCGTGGGTATGTACGGAAATAATGGCTTTGTCGATGTTGGGTACGCGTTCATACAGGCCg > 1:2320084/1‑68 (MQ=255) ccAAATCGTCGTGGGTATGTACGGAAATAATGGCTTTGTCGATGTTGGGTACGCGTTCATACAGGCCg > 1:89100/1‑68 (MQ=255) ccAAATCGTCGTGGGTATGTACGGAAATAATGGCTTTGTCGATGTTGGGTACGCGTTCATACAGGCCg > 1:245435/1‑68 (MQ=255) ccAAATCGTCGTGGGTATGTACGGAAATAATGGCTTTGTCGATGTTGGGTACGCGTTCATACAGGCCg > 1:2223609/1‑68 (MQ=255) ccAAATCGTCGTGGGTATGTACGGAAATAATGGCTTTGTCGATGTTGGGTACACGTTCATACAGGCCg > 1:2757782/1‑68 (MQ=255) tcgtcgTGGGTATGTACGGAGATAATGGCTTTGTCGATGTTAGGCACGCGTTCATACAGGCCGCtgatg > 1:939388/1‑69 (MQ=255) tGTACGGAGATAATGGCTTTGTCGATGTTAGGCAcgc > 1:215848/1‑37 (MQ=255) tGTACGGAGATAATGGCTTTGTCGATGTTAGGCACGCGtt < 1:1981967/40‑1 (MQ=255) tGTACGGAGATAATGGCTTTGTCGATGTTAGGCACGCGtt < 1:1920646/40‑1 (MQ=255) tGTACGGAGATAATGGCTTTGTCGATGTTAGGCACGCGtt < 1:2556623/40‑1 (MQ=255) tGTACGGAGATAATGGCTTTGTCGATGTTAGGCACGCGtt < 1:1663497/40‑1 (MQ=255) tGTACGGAGATAATGGCTTTGTCGATGTTAGGCACGCGtt < 1:2288463/40‑1 (MQ=255) tGTACGGAGATAATGGCTTTGTCGATGTTAGGCACGCGtt < 1:92317/40‑1 (MQ=255) tGTACGGAGATAATGGCTTTGTCGATGTTAGGCACGCGtt < 1:1206193/40‑1 (MQ=255) tGTACGGAGATAATGGCTTTGTCGATGTTAGGCACGCGTTCATACAg > 1:3407171/1‑47 (MQ=255) tGTACGGAGATAATGGCTTTGTCGATGTTAGGCACGCGTTCATACAg > 1:2059776/1‑47 (MQ=255) tGTACGGAGATAATGGCTTTGTCGATGTTAGGCACGCGTTCATACAg > 1:649631/1‑47 (MQ=255) tGTACGGAGATAATGGCTTTGTCGATGTTAGGCACGCGTTCATACAg > 1:2359463/1‑47 (MQ=255) tGTACGGAGATAATGGCTTTGTCGATGTTAGGCACGCGTTCATACAg > 1:1532439/1‑47 (MQ=255) tGTACGGAGATAATGGCTTTGTCGATGTTAGGCACGCGTTCATACAg > 1:2486837/1‑47 (MQ=255) tGTACGGAGATAATGGCTTTGTCGATGTTAGGCACACGtt < 1:1251236/40‑1 (MQ=255) cGGAGATAATGGCTTTGTCGATGTTAGGCACGCGTTCATACAGGCCGCTGATGATTCCGTCGAACTCa < 1:986783/68‑1 (MQ=255) cGGAGATAATGGCTTTGTCGATGTTAGGCACGCGTTCATACAGGCCGCTGATGATTCCGGCGAACTCaa < 1:2062526/69‑1 (MQ=255) cGGAGATAATGGCTTTGTCGATGTTAGGCACGCGTTCATACAGGCCGCTGATGATTCCGGCGAACTCa < 1:541021/68‑1 (MQ=255) cGGAGATAATGGCTTTGTCGATGTTAGGCACGCGTTCATACAGGCCGCTGATGATTCCGGCGAACTCa < 1:508036/68‑1 (MQ=255) cGGAGATAATGGCTTTGTCGATGTTAGGCACGCGTTCATACAGGCCGCTGATGATTCCGGCGAACTCa < 1:3338685/68‑1 (MQ=255) cGGAGATAATGGCTTTGTCGATGTTAGGCACGCGTTCATACAGGCCGCTGATGATTCCGGCGAACTCa < 1:3038067/68‑1 (MQ=255) cGGAGATAATGGCTTTGTCGATGTTAGGCACGCGTTCATACAGGCCGCTGATGATTCCGGCGAACTCa < 1:1631975/68‑1 (MQ=255) cGGAGATAATGGCTTTGTCGATGTTAGGCACGCGTTCATACAGGCCGCTGATGATTCCGGCGAACTCa < 1:2237079/68‑1 (MQ=255) cGGAGATAATGGCTTTGTCGATGTTAGGCACGCGTTCATACAGGCCGCTGATGATTCCGGCGAACTCa < 1:954653/68‑1 (MQ=255) gagaTAATGGCTTTGTCGATGTTAGGCACTCGTTCATACAGGCCGCTGATGATTCCGGCGAACTCAAAc > 1:22351/1‑69 (MQ=255) gagaTAATGGCTTTGTCGATGTTAGGCACGCGTTCATACAGGCCGCTGATGATTCCGGCGAACTCAAAc > 1:510134/1‑69 (MQ=255) gagaTAATGGCTTTGTCGATGTTAGGCACGCGTTCATACAGGCCGCTGATGATTCCGGCGAACTCAAAc > 1:742100/1‑69 (MQ=255) gaTAATGGCTTTGTCGATGTTAGGCACGCGTTCATACAGGCCGCTGATGATTCCGGCGAACTCAAACgg < 1:1389715/69‑1 (MQ=255) gaTAATGGCTTTGTCGATGTTAGGCACGCGTTCATACAGGCCGCTGATGATTCCGGCGAACTCAAACgg < 1:1567606/69‑1 (MQ=255) gaTAATGGCTTTGTCGATGTTAGGCACGCGTTCATACAGGCCGCTGATGATTCCGGCGAACTCAAACgg < 1:1751032/69‑1 (MQ=255) | CCGGCATGTACCGCCGCCAGTGAGTTTCCGACCGCCAGGCCCAAATCGTCGTGGGTATGTACGGAGATAATGGCTTTGTCGATGTTAGGCACGCGTTCATACAGGCCGCTGATGATTCCGGCGAACTCAAACGG > minE/67608‑67741 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |