| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 615,142 | A→C | 42.2% | E264A (GAA→GCA) | serC → | 3‑phosphoserine/phosphohydroxythreonine aminotransferase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 615,142 | 0 | A | C | 42.2% | 25.0 / 94.4 | 90 | E264A (GAA→GCA) | serC | 3‑phosphoserine/phosphohydroxythreonine aminotransferase |
| Reads supporting (aligned to +/- strand): ref base A (16/36); new base C (11/27); total (27/63) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.07e-01 | |||||||||||
TATCTGGTCTGGTCTTTAAATGGCTGAAAGCGAACGGCGGTGTAGCTGAAATGGATAAAATCAATCAGCAAAAAGCAGAACTGCTATATGGGGTGATTGATAACAGCGAT > minE/615094‑615203 | tatctGGTCTGGTCTTTAAATGGCTGAAAGCGAACGGCGGTGTAGCTGAAATg > 1:201560/1‑53 (MQ=255)tatctGGTCTGGTCTTTAAATGGCTGAAAGCGAACGGCGGTGTAGCTGAAATGGATAAAatcaatca > 1:2363037/1‑67 (MQ=255)tatctGGTCTGGTCTTTAAATGGCTGAAAGCGAACGGCGGTGTAGCTGAAATGGATAAAATCAATCAg > 1:1719596/1‑68 (MQ=255)tatctGGTCTGGTCTTTAAATGGCTGAAAGCGAACGGCGGTGTAGCTGAAATGGATAAAATCAATCAg > 1:2506641/1‑68 (MQ=255) tggtctTTAAATGGCTGAAAGCGAACGGCGGTGTAGCTGAAATGGATAAAATCAATCAGCAAAAAGCAg < 1:1382052/69‑1 (MQ=255) tggtctTTAAATGGCTGAAAGCGAACGGCGGTGTAGCTGAAATGGATAAAATCAATCAGCAAAAAGCAg < 1:1910901/69‑1 (MQ=255) tggtctTTAAATGGCTGAAAGCGAACGGCGGTGTAGCTGAAATGGATAAAATCAATCAGCAAAAAGCAg < 1:1944513/69‑1 (MQ=255) tggtctTTAAATGGCTGAAAGCGAACGGCGGTGTAGCTGAAATGGATAAAATCAATCAGCAAAAAGCAg < 1:3359934/69‑1 (MQ=255) tggtctTTAAATGGCTGAAAGCGAACGGCGGTGTAGCTGAAATGGATAAAATCAATCAGCAAAAAGCAg < 1:2750113/69‑1 (MQ=255) tggtctTTAAATGGCTGAAAGCGAACGGCGGTGTAGCTGAAATGGATAAAATCAATCAGCAAAAAGCAg < 1:2727206/69‑1 (MQ=255) tggtctTTAAATGGCTGAAAGCGAACGGCGGTGTAGCTGAAATGGATAAAATCAATCAGCAAAAAGCAg < 1:613084/69‑1 (MQ=255) tggtctTTAAATGGCTGAAAGCGAACGGCGGTGTAGCTGAAATGGATAAAATCAATCAGCAAAAAGCAg < 1:710005/69‑1 (MQ=255) tggtctTTAAATGGCTGAAAGCGAACGGCGGTGTAGCTGAAATGGATAAAATCAATCAGCAAAAAGCAg < 1:2713493/69‑1 (MQ=255) tggtctTTAAATGGCTGAAAGCGAACGGCGGTGTAGCTGAAATGGATAAAATCAATCAGCAAAAAGCAg < 1:2533610/69‑1 (MQ=255) ctTTAAATTGCTGAAAGCGAACGGCGGTGTAGCTGAAATGGATaa < 1:727036/45‑1 (MQ=255) ctTTAAATGGCTGAAAGCGAACTGCGGTGTAGCTGAAATGGATaa > 1:498574/1‑45 (MQ=255) ctTTAAATGGCTGAAAGCGAACGGCGGTGTAGCTGAAATGGATaa > 1:1093879/1‑45 (MQ=255) ctTTAAATGGCTGAAAGCGAACGGCGGTGTAGCTGAAATGGATaa > 1:2472600/1‑45 (MQ=255) ctTTAAATGGCTGAAAGCGAACGGCGGTGTAGCTGAAATGGATaa > 1:2509690/1‑45 (MQ=255) ctTTAAATGGCTGAAAGCGAACGGCGGTGTAGCTGAAATGGATaa < 1:2229091/45‑1 (MQ=255) ctTTAAATGGCTGAAAGCGAACGGCGGTGTAGCTGAAATGGATaa < 1:3118604/45‑1 (MQ=255) ctTTAAATGGCTGAAAGCGAACGGCGGTGTAGCTGAAATGGATaa < 1:1131676/45‑1 (MQ=255) ctTTAAATGGCTGAAAGCGAACGGCGGTGTAGCTGAAATGGATaa < 1:1633006/45‑1 (MQ=255) ctTTAAATGGCTGAAAGCGAACGGCGGTGTAGCTGAAATGGATaa > 1:1228740/1‑45 (MQ=255) ctTTAAATGGCTGAAAGCGAACGGCGGTGTAGCTGAAATGGATaa > 1:491419/1‑45 (MQ=255) ctTTAAATGGCTGAAAGCGAACGGCGGTGTAGCTGAAATGGATaa < 1:1467780/45‑1 (MQ=255) ctTTAAATGGCTGAAAGCGAACGGCGGTGTAGCTGAAATGGATaa < 1:1507892/45‑1 (MQ=255) aaTGGCTGAAAGCGAACGGCGGTGTAGCTGAAATGGATAAAATCAATCAGCAAAAAGCAGAACTGCta < 1:3202281/68‑1 (MQ=255) aaTGGCTGAAAGCGAACGGCGGTGTAGCTGAAATGGATAAAATCAATCAGCAAAAAGCAGAACTGCta < 1:2585734/68‑1 (MQ=255) aaTGGCTGAAAGCGAACGGCGGTGTAGCTGAAATGGATAAAATCAATCAGCAAAAAGCAGAACTGCta < 1:3394545/68‑1 (MQ=255) aaTGGCTGAAAGCGAACGGCGGTGTAGCTGAAATGGATAAAATCAATCAGCAAAAAGCAGAACTGCta < 1:3375483/68‑1 (MQ=255) aaTGGCTGAAAGCGAACGGCGGTGTAGCTGAAATGGATAAAATCAATCAGCAAAAAGCAGAACTGCta < 1:2632714/68‑1 (MQ=255) aaTGGCTGAAAGCGAACGGCGGTGTAGCTGAAATGGATAAAATCAATCAGCAAAAAGCAGAACTGCta < 1:1529405/68‑1 (MQ=255) aaTGGCTGAAAGCGAACGGCGGTGTAGCTGAAATGGATAAAATCAATCAGCAAAAAGCAGAACTGCta < 1:3279989/68‑1 (MQ=255) aaTGGCTGAAAGCGAACGGCGGTGTAGCTGAAATGGATAAAATCAATCAGCAAAAAGCAGAACTGCta < 1:2027381/68‑1 (MQ=255) aaTGGCTGAAAGCGAACGGCGGTGTAGCTGAAATGGATAAAATCAATCAGCAAAAAGCAGAACTGCta < 1:2023596/68‑1 (MQ=255) aaTGGCTGAAAGCGAACGGCGGTGTAGCTGAAATGGATAAAATCAATCAGCAAAAAGCAGAACTGCta < 1:3207136/68‑1 (MQ=255) aaTGGCTGAAAGCGAACGGCGGTGTAGCTGAAATGGATAAAATCAATCAGCAAAAAGCAGAACTGCta < 1:1692041/68‑1 (MQ=255) aaTGGCTGAAAGAGAACGGCGGGGTAGCTGAAATGGATAAAATCAATCAGCAAAAAGCAGAACTGCta < 1:315573/68‑1 (MQ=255) gCTGAAAGCGAACGGCGGTGTAGCTGAAATGGATAAAATCAATCAGCAAAAAGCAGAACTGCTATATgg > 1:3078506/1‑69 (MQ=255) gCTGAAAGCGAACGGCGGTGTAGCTGAAATGGATAAAATCAATCAGCAAAAAGCAGAACTGCTATATgg > 1:2911288/1‑69 (MQ=255) gCTGAAAGCGAACGGCGGTGTAGCTGAAATGGATAAAATCAATCAGCAAAAAGCAGAACTGCTATATgg > 1:3144157/1‑69 (MQ=255) gCTGAAAGCGAACGGCGGTGTAGCTGAAATGGATAAAATCAATCAGCAAAAAGCAGAACTGCTATATgg > 1:1994033/1‑69 (MQ=255) gCTGAAAGCGAACGGCGGTGTAGCTGAAATGGATAAAATCAATCAGCAAAAAGCAGAACTGCTATATgg > 1:1067910/1‑69 (MQ=255) gAAAGCGAACGGCGGTGTAGCTGAAATGGATAAAATCAATCAGCAAAAAGCGGAACTGCTATATGGGGt < 1:2702281/69‑1 (MQ=255) gAAAGCGAACGGCGGTGTAGCTGAAATGGATAAAATCAATCAGCAAAAAGCAGAACTGCTATATGGGGt < 1:2632136/69‑1 (MQ=255) gAAAGCGAACGGCGGTGTAGCTGAAATGGATAAAATCAATCAGCAAAAAGCAGAACTGCTATATGGGGt < 1:297780/69‑1 (MQ=255) gAAAGCGAACGGCGGTGTAGCTGAAATGGATAAAATCAATCAGCAAAAAGCAGAACTGCTATATGGGGt < 1:3224266/69‑1 (MQ=255) gAAAGCGAACGGCGGTGTAGCTGAAATGGATAAAATCAATCAGCAAAAAGCAGAACTGCTATATGGGGt < 1:1162490/69‑1 (MQ=255) gAAAGCGAACGGCGGTGTAGCTGAAATGGATAAAATCAATCAGCAAAAAGCAGAACTGCTATATGGGGt < 1:2296259/69‑1 (MQ=255) aaGCGAACGGCGGTGTGGCTGCAATGGATAAGATCAATCGGCAAAAAGCAGAACTGCt < 1:1169954/58‑1 (MQ=255) aaGCGAACGGCGGTGTGGCTGCAATGGATAAGATCAATCGGCAAAAAGCAGAACTGCt < 1:319995/58‑1 (MQ=255) aaGCGAACGGCGGTGTGGCTGCAATGGATAAGATCAATCGGCAAAAAGCAGAACTGCt < 1:2647785/58‑1 (MQ=255) aaGCGAACGGCGGTGTGGCTGCAATGGATAAGATCAATCGGCAAAAAGCAGAACTGCt < 1:1592440/58‑1 (MQ=255) aaGCGAACGGCGGTGTGGCTGCAATGGATAAGATCAATCGGCAAAAAGCAGAACTGCt < 1:1545554/58‑1 (MQ=255) aaGCGAACGGCGGTGTGGCTGCAATGGATAAGATCAATCGGCAAAAAGCAGAACTGCt < 1:1501116/58‑1 (MQ=255) aaGCGAACGGCGGTGTGGCTGCAATGGATAAGATCAATCGGCAAAAAGCAGAACTGCt < 1:1350975/58‑1 (MQ=255) aaGCGAACGGCGGTGTGGCTGCAATGGATAAGATCAATCGGCAAAAAGCAGAACTGCt < 1:379234/58‑1 (MQ=255) aaGCGAACGGCGGTGTGGCTGCAATGGATAAGATCAATCGGCAAAAAGCAGAACTGCt < 1:453140/58‑1 (MQ=255) aaGCGAACGGCGGTGTGGCTGCAATGGATAAGATCAATCGGCAAAAAGCAGAACTGCt < 1:460258/58‑1 (MQ=255) aaGCGAACGGCGGTGTGGCTGCAATGGATAAGATCAATCGGCAAAAAGCAGAACTGCt < 1:1757322/58‑1 (MQ=255) aaGCGAACGGCGGTGTGGCTGCAATGGATAAGATCAATCGGCAAAAAGCAGAACTGCt < 1:547955/58‑1 (MQ=255) aaGCGAACGGCGGTGTGGCTGCAATGGATAAGATCAATCGGCAAAAAGCAGAACTGCt < 1:622849/58‑1 (MQ=255) aaGCGAACGGCGGTGTGGCTGCAATGGATAAGATCAATCGGCAAAAAGCAGAACTGCt < 1:1136373/58‑1 (MQ=255) aaGCGAACGGCGGTGTGGCTGCAATGGATAAGATCAATCGGCAAAAAGCAGAACTGCt < 1:1104059/58‑1 (MQ=255) aaGCGAACGGCGGTGTGGCTGCAATGGATAAGATCAATCGGCAAAAAGCAGAACTGCt < 1:873791/58‑1 (MQ=255) aaGCGAACGGCGGTGTGGCTGCAATGGATAAGATCAATCGGCAAAAAGCAGAACTGCt < 1:919356/58‑1 (MQ=255) aaGCGAACGGCGGTGTGGCTGCAATGGATAAGATCAATCGGCAAAAAGCAGAACTGCt < 1:2833632/58‑1 (MQ=255) aaGCGAACGGCGGTGTGGCTGCAATGGATAAGATCAATCGGCAAAAAGCAGAACTGCt < 1:2019909/58‑1 (MQ=255) aaGCGAACGGCGGTGTGGCTGCAATGGATAAGATCAATCGGCAAAAAGCAGAACTGCt < 1:3130137/58‑1 (MQ=255) aaGCGAACGGCGGTGTGGCTGCAATGGATAAGATCAATCGGCAAAAAGCAGAACTGCt < 1:2185599/58‑1 (MQ=255) aaGCGAACGGCGGTGTGGCTGCAATGGATAAGATCAATCGGCAAAAAGCAGAACTGCt < 1:2947172/58‑1 (MQ=255) aaGCGAACGGCGGTGTGGCTGCAATGGATAAGATCAATCGGCAAAAAGCAGAACTGCt < 1:2267373/58‑1 (MQ=255) aaGCGAACGGCGGTGTGGCTGCAATGGATAAGATCAATCGGCAAAAAGCAGAACTGCt < 1:2460681/58‑1 (MQ=255) aaGCGAACGGCGGTGTGGCTGCAATGGATAAGATCAATCGGCAAAAAGCAGAACTGCt < 1:3019674/58‑1 (MQ=255) aaGCGAACGGCGGTGTGGCTGCAATGGATAAGATCAATCGGCAAAAAGCAGAACTGCt < 1:1996429/58‑1 (MQ=255) gtAGCTGAAATGGATAAAATCAATCAGCAAAAAGCAGAACTGCTATATGGGGTGATTGATAACAGCGAt < 1:2706987/69‑1 (MQ=255) gtAGCTGAAATGGATAAAATCAATCAGCAAAAAGCAGAACTGCTATATGGGGTGATTGATAACAGCGAt > 1:1329035/1‑69 (MQ=255) gCTGCAATGGATAAGATCAATCGGCAAAAAGCAGAACTGCt < 1:2983883/41‑1 (MQ=37) gCTGCAATGGATAAGATCAATCAGCAAAAAGCAGAACTGCtgt > 1:2514159/1‑41 (MQ=37) gCTGCAATGGATAAGATCAATCAGCAAAAAGCAGAACTGCtgt > 1:2489990/1‑41 (MQ=37) gCTGCAATGGATAAGATCAATCAGCAAAAAGCAGAACTGCtgt > 1:2985294/1‑41 (MQ=37) gCTGCAATGGATAAGATCAATCAGCAAAAAGCAGAACTGCtgt > 1:780744/1‑41 (MQ=37) gCTGCAATGGATAAGATCAATCAGCAAAAAGCAGAACTGCtgt > 1:2644190/1‑41 (MQ=37) gCTGCAATGGATAAGATCAATCAGCAAAAAGCAGAACTGCtgt > 1:2269432/1‑41 (MQ=37) gCTGCAATGGATAAGATCAATCAGCAAAAAGCAGAACTGCtgt > 1:429285/1‑41 (MQ=37) gCTGCAATGGATAAGATCAATCAGCAAAAAGCAGAACTGCtgt > 1:3422945/1‑41 (MQ=37) gCTGCAATGGATAAGATCAATCAGCAAAAAGCAGAACTGCtgt > 1:3130839/1‑41 (MQ=37) gCTGCAATGGATAAGATCAATCAGCAAAAAGCAGAACTGCtgt > 1:1833439/1‑41 (MQ=37) gCTGCAATGGATAAGATCAATAAGCAAAAAGCAGAACTGCtgt > 1:1406881/1‑41 (MQ=37) | TATCTGGTCTGGTCTTTAAATGGCTGAAAGCGAACGGCGGTGTAGCTGAAATGGATAAAATCAATCAGCAAAAAGCAGAACTGCTATATGGGGTGATTGATAACAGCGAT > minE/615094‑615203 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |