| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 616,302 | T→C | 40.8% | G264G (GGT→GGC) | aroA → | 5‑enolpyruvylshikimate‑3‑phosphate synthetase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 616,302 | 0 | T | C | 40.8% | 34.7 / 67.5 | 93 | G264G (GGT→GGC) | aroA | 5‑enolpyruvylshikimate‑3‑phosphate synthetase |
| Reads supporting (aligned to +/- strand): ref base T (32/23); new base C (16/22); total (48/45) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.44e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
GCATCTTCGGCTTCTTACTTTCTGGCAGCAGCAGCAATCAAAGGCGGCACTGTAAAAGTGACCGGTATTGGACGTAACAGTATGCAGGGTGATATTCGCTTTGCTGATGTGCTGGAAAAAATGGGC > minE/616237‑616362 | gCATCTTCGGCTTCTTACTTTCTGGCAGCAGCAGCAATCAAAGGCGGCACTGTAAAAGTGACCGGTAt > 1:2883604/1‑68 (MQ=255)gCATCTTCGGCTTCTTACTTTCTGGCAGCAGCAGCAATCAAAGGCGGCACTGTAAAAGTGACCGGTAt > 1:174339/1‑68 (MQ=255)gCATCTTCGGCTTCTTACTTTCTGGCAGCAGCAGCAATCAAAGGCGGCACTGTAAAAGTGACCGGTAt > 1:1456507/1‑68 (MQ=255)gCATCTTCGGCTTCTTACTTTCTGGCAGCAGCAGCAATCAAAGGCGGCACTGTAAAAGTGACCGGTAt > 1:1399812/1‑68 (MQ=255) cGGCTTCTTACTTTCTGGCAGCAGCAGCAATCAAAGGCGGCACTGTAAAAGTGACCGGTATTGGACGt > 1:252979/1‑68 (MQ=255) cGGCTTCTTACTTTCTGGCAGCAGCAGCAATCAAAGGCGGCACTGTAAAAGTGACCGGTATTGGACGt > 1:24748/1‑68 (MQ=255) cGGCTTCTTACTTTCTGGCAGCAGCAGCAATCAAAGGCGGCACTGTAAAAGTGACCGGTATTGGACGt > 1:1536115/1‑68 (MQ=255) cGGCTTCTTACTTTCTGGCAGCAGCAGCAATCAAAGGCGGCACTGTAAAAGTGACCGGTATTGGACGt > 1:3054798/1‑68 (MQ=255) cGGCTTCTTACTTTCTGGCAGCAGCAGCAATCAAAGGCGGCACTGTAAAAGTGACCGGTATTGGACGt > 1:1471743/1‑68 (MQ=255) cGGCTTCTTACTTTCTGGCAGCAGCAGCAATCAAAGGCGGCACTGTAAAAGTGACCGGTATTGGACGt > 1:2043191/1‑68 (MQ=255) cGGCTTCTTACTTTCTGGCAGCAGCAGCAATCAAAGGCGGCACTGTAAAAGTGACCGGTATTGGACGt > 1:446181/1‑68 (MQ=255) cGGCTTCTTACTTTCTGGCAGCAGCAGCAATCAAAGGCGGCACTGTAAAAGTGACCGGTATTGGACGt > 1:1034759/1‑68 (MQ=255) cGGCTTCTTACTTTCTGGCAGCAGCAGCAATCAAAGGCGGCACTGTAAAAGTGACCGGTATTGGACGt > 1:1028552/1‑68 (MQ=255) ttcttACTTTCTGGCAGCAGCAGGAATCAAAGGCGGCACTGTAAAAGTGACCGGTATTGGACGTAACAg < 1:593119/69‑1 (MQ=255) aCTTTCTGGCAGCAGCAGCAATCAAAGGCGGCACTGTAAAAGTGACCGGTATTGGACGTAACAGTATgg > 1:1232974/1‑68 (MQ=255) agcagcagcaATCAGAGGCGGCACTGTAAAAGTGACCGGCATTGGACGCAACAGTATGCAGGGTGatat > 1:2010829/1‑69 (MQ=255) agcagcagcaATCAGAGGCGGCACTGTAAAAGTGACCGGCATTGGACGCAACAGTATGCAGGGTGatat > 1:2267555/1‑69 (MQ=255) agcagcagcaATCAGAGGCGGCACTGTAAAAGTGACCGGCATTGGACGCAACAGTATGCAGGGTGatat > 1:2421827/1‑69 (MQ=255) agcagcagcaATCAGAGGCGGCACTGTAAAAGTGACCGGCATTGGACGCAACAGTATGCAGGGTGatat > 1:1925338/1‑69 (MQ=255) agcagcagcaATCAGAGGCGGCACTGTAAAAGTGACCGGCATTGGACGCAACAGTATGCAGGGTGatat > 1:3013248/1‑69 (MQ=255) agcagcagcaATCAGAGGCGGCACTGTAAAAGTGACCGGCATTGGACGCAACAGTATGCAGGGTGatat > 1:313383/1‑69 (MQ=255) agcagcagcaATCAGAGGCGGCACTGTAAAAGTGACCGGCATTGGACGCAACAGTATGCAGGGTGatat > 1:3246915/1‑69 (MQ=255) agcagcagcaATCAGAGGCGGCACTGTAAAAGTGACCGGCATTGGACGCAACAGTATGCAGGGTGatat > 1:509240/1‑69 (MQ=255) agcagcagcaATCAGAGGCGGCACTGTAAAAGTGACCGGCATTGGACGCAACAGTATGCAGGGTGatat > 1:560926/1‑69 (MQ=255) agcagcagcaATCAGAGGCGGCACTGTAAAAGTGACCGGCATTGGACGCAACAGTATGCAGGGTGatat > 1:67034/1‑69 (MQ=255) agcagcagcaATCAGAGGCGGCACTGTAAAAGTGACCGGCATTGGACGCAACAGTATGCAGGGTGatat > 1:1807630/1‑69 (MQ=255) agcagcagcaATCAGAGGCGGCACTGTAAAAGTGACCGGCATTGGACGCAACAGTATGCAGGGTGatat > 1:1124358/1‑69 (MQ=255) agcagcagcaATCAGAGGCGGCACTGTAAAAGTGACCGGCATTGGACGCAACAGTATGCAGGGTGatat > 1:1554459/1‑69 (MQ=255) agcagcagcaATCAGAGGCGGCACTGTAAAAGTGACCGGCATTGGACGCAACAGTATGCAGGGTGata > 1:2383134/1‑68 (MQ=255) agcagcagcaATCAGAGGCGGCACTGTAAAAGTGACCGGCATTGGACGCAACAGTATGCAGGGTGata > 1:2714273/1‑68 (MQ=255) agcagcagcaATCAGAGGCGGCACTGTAAAAGTGACCGGCATTGGACGCAACAGTATGCAGGGTGata > 1:2777840/1‑68 (MQ=255) agcagcagcaATCAAAGGCGGCACTGTAAAAGTGACCGGTATTGGACGTAACAGTATGCAGGGTGa < 1:1262556/66‑1 (MQ=255) agcagcagcaATCAAAGGCGGCACTGTAAAAGTGACCGGTATTGGACGTAACAGTATGCAGGGTGa < 1:1267496/66‑1 (MQ=255) agcagcagcaATCAAAGGCGGCACTGTAAAAGTGACCGGTATTGGACGTAACAGTATGCAGGGTGa < 1:3346271/66‑1 (MQ=255) agcagcagcaATCAAAGGCGGCACTGTAAAAGTGACCGGTATTGGACGTAACAGTATGCAGGGTGa < 1:3244642/66‑1 (MQ=255) agcagcagcaATCAAAGGCGGCACTGTAAAAGTGACCGGTATTGGACGTAACAGTATGCAGGGTGa < 1:3222062/66‑1 (MQ=255) agcagcagcaATCAAAGGCGGCACTGTAAAAGTGACCGGTATTGGACGTAACAGTATGCAGGGTGa < 1:3112615/66‑1 (MQ=255) agcagcagcaATCAAAGGCGGCACTGTAAAAGTGACCGGTATTGGACGTAACAGTATGCAGGGTGa < 1:570197/66‑1 (MQ=255) agcagcagcaATCAAAGGCGGCACTGTAAAAGTGACCGGTATTGGACGTAACAGTATGCAGGGTGa < 1:2979428/66‑1 (MQ=255) agcagcagcaATCAAAGGCGGCACTGTAAAAGTGACCGGTATTGGACGTAACAGTATGCAGGGTGa < 1:2801626/66‑1 (MQ=255) agcagcagcaATCAAAGGCGGCACTGTAAAAGTGACCGGTATTGGACGTAACAGTATGCAGGGTGa < 1:777701/66‑1 (MQ=255) agcagcagcaATCAAAGGCGGCACTGTAAAAGTGACCGGTATTGGACGTAACAGTATGCAGGGTGa < 1:840226/66‑1 (MQ=255) agcagcagcaATCAAAGGCGGCACTGTAAAAGTGACCGGTATTGGACGTAACAGTATGCAGGGTGa < 1:1565616/66‑1 (MQ=255) agcagcagcaATCAAAGGCGGCACTGTAAAAGTGACCGGTATTGGACGTAACAGTATGCAGGGTGa < 1:8873/66‑1 (MQ=255) agcagcagcaATCAAAGGCGGCACTGTAAAAGTGACCGGTATTGGACGTAACAGTATGCAGGGTGa < 1:2011404/66‑1 (MQ=255) agcagcagcaATCAAAGGCGGCACTGTAAAAGTGACCGGTAGTGGACGTAACAGTATGCAGGGTGa < 1:2714465/66‑1 (MQ=255) agcagcagcaATCAAAGGCGGAACTGTAAAAGTGACCGGTATTGGACGTAACAGTATGCAGGGTGa < 1:735889/66‑1 (MQ=255) agcaATCAAAGGCGGCACTGTAAAAGTGACCGGTATTGGACGTAACAGTATGc > 1:2409339/1‑53 (MQ=255) agcaATCAAAGGCGGCACTGTAAAAGTGACCGGTATTGGACGTAACAGTATGCAGGGTGATATTCGCtt > 1:3363169/1‑69 (MQ=255) agcaATCAAAGGCGGCACTGTAAAAGTGACCGGTATTGGACGTAACAGTATGCAGGGTGATATTCGCtt > 1:2985844/1‑69 (MQ=255) agcaATCAAAGGCGGCACTGTAAAAGTGACCGGTATTGGACGTAACAGTATGCAGGGTGATATTCGCt > 1:517589/1‑68 (MQ=255) agcaATCAAAGGCGGCACTGTAAAAGTGAACGGTATTGGACGTAACAGTATGCAGGGTGATATTCGc > 1:769268/1‑67 (MQ=255) aTCAAAGGCGGCACTGTAAAAGTGACCGGTATTGGACGTAACAGTATGCAGGGTGATATTCGCTTTGCt < 1:1151917/69‑1 (MQ=255) aTCAAAGGCGGCACTGTAAAAGTGACCGGTATTGGACGTAACAGTATGCAGGGTGATATTCGCTTTGCt < 1:1189581/69‑1 (MQ=255) aTCAAAGGCGGCACTGTAAAAGTGACCGGTATTGGACGTAACAGTATGCAGGGTGATATTCGCTTTGCt < 1:1002596/69‑1 (MQ=255) aTCAAAGGCGGCACTGTAAAAGTGACCGGTATTGGACGTAACAGTATGCAGGGTGATATTCGCTTTGCt < 1:1560440/69‑1 (MQ=255) aTCAAAGGCGGCACTGTAAAAGTGACCGGTATTGGACGTAACAGTATGCAGGGTGATATTCGCTTTGCt < 1:1459082/69‑1 (MQ=255) aaaGGCGGCACTGTAAAAGTGACCGGTATTGGACGTAACAGTATGCAGGGTGATATTCGCTTTGCt < 1:208788/66‑1 (MQ=255) ggcACTGTAAAAGTGACCGGTATTGGACGTAACAGTATGCAGGGTGATATTCGCTTTGCTGATGTGCt > 1:1706996/1‑68 (MQ=255) ggcACTGTAAAAGTGACCGGTATTGGACGTAACAGTATGCAGGGTGATATTCGCTTTGCTGATGTGCt > 1:2065324/1‑68 (MQ=255) aaaGTGACCGGTATTGGACGTAACAGTATGCAGGGTGATATTCGc > 1:2015343/1‑45 (MQ=255) aaaGTGACCGGTATTGGACGTAACAGTATGCAGGGTGATATTCGCTTTGCTGATGTGCt > 1:1854663/1‑59 (MQ=255) aaaGTGACCGGTATTGGACGTAACAGTATGCAGGGTGATATTCGCTTTGCTGATGTGCTGGAAAAAATg > 1:1466879/1‑69 (MQ=255) aaaGTGACCGGTATTGGACGTAACAGTATGCAGGGTGATATTCGCTTTGCTGATGTGCTGGAAAAAATg > 1:417769/1‑69 (MQ=255) aaaGTGACCGGTATTGGACGTAACAGTATGCAGGGTGATATTCGCTTTGCTGATGTGCTGGAAAAAATg > 1:1772484/1‑69 (MQ=255) aaaGTGACCGGCATTGGACGCAACAGTATGCAGGGTGATATTCGCTTTGCTGATGTTCTGGAAAAAAt < 1:3118589/68‑1 (MQ=255) aaaGTGACCGGCATTGGACGCAACAGTATGCAGGGTGATATTCGCTTTGCTGATGTTCTGGAAAAAAt < 1:87519/68‑1 (MQ=255) aaaGTGACCGGCATTGGACGCAACAGTATGCAGGGTGATATTCGCTTTGCTGATGTTCTGGAAAAAAt < 1:85689/68‑1 (MQ=255) aaaGTGACCGGCATTGGACGCAACAGTATGCAGGGTGATATTCGCTTTGCTGATGTTCTGGAAAAAAt < 1:840297/68‑1 (MQ=255) aaaGTGACCGGCATTGGACGCAACAGTATGCAGGGTGATATTCGCTTTGCTGATGTTCTGGAAAAAAt < 1:83470/68‑1 (MQ=255) aaaGTGACCGGCATTGGACGCAACAGTATGCAGGGTGATATTCGCTTTGCTGATGTTCTGGAAAAAAt < 1:822936/68‑1 (MQ=255) aaaGTGACCGGCATTGGACGCAACAGTATGCAGGGTGATATTCGCTTTGCTGATGTTCTGGAAAAAAt < 1:810215/68‑1 (MQ=255) aaaGTGACCGGCATTGGACGCAACAGTATGCAGGGTGATATTCGCTTTGCTGATGTTCTGGAAAAAAt < 1:1865493/68‑1 (MQ=255) aaaGTGACCGGCATTGGACGCAACAGTATGCAGGGTGATATTCGCTTTGCTGATGTTCTGGAAAAAAt < 1:2435075/68‑1 (MQ=255) aaaGTGACCGGCATTGGACGCAACAGTATGCAGGGTGATATTCGCTTTGCTGATGTTCTGGAAAAAAt < 1:1593499/68‑1 (MQ=255) aaaGTGACCGGCATTGGACGCAACAGTATGCAGGGTGATATTCGCTTTGCTGATGTTCTGGAAAAAAt < 1:1884069/68‑1 (MQ=255) aaaGTGACCGGCATTGGACGCAACAGTATGCAGGGTGATATTCGCTTTGCTGATGTTCTGGAAAAAAt < 1:2659054/68‑1 (MQ=255) aaaGTGACCGGCATTGGACGCAACAGTATGCAGGGTGATATTCGCTTTGCTGATGTTCTGGAAAAAAt < 1:2199948/68‑1 (MQ=255) aaaGTGACCGGCATTGGACGCAACAGTATGCAGGGTGATATTCGCTTTGCTGATGTTCTGGAAAAAAt < 1:363539/68‑1 (MQ=255) aaaGTGACCGGCATTGGACGCAACAGTATGCAGGGTGATATTCGCTTTGCTGATGTTCTGGAAAAAAt < 1:3436207/68‑1 (MQ=255) aaaGTGACCGGCATTGGACGCAACAGTATGCAGGGTGATATTCGCTTTGCTGATGTTCTGGAAAAAAt < 1:3430033/68‑1 (MQ=255) aaaGTGACCGGCATTGGACGCAACAGTATGCAGGGTGATATTCGCTTTGCTGATGTTCTGGAAAAAAt < 1:2192661/68‑1 (MQ=255) aaaGTGACCGGCATTGGACGCAACAGTATGCAGGGTGATATTCGCTTTGCTGATGTTCTGGAAAAAAt < 1:2313782/68‑1 (MQ=255) aaaGTGACCGGCATTGGACGCAACAGTATGCAGGGTGATATTCGCTTTGCTGATGTTCTGGAAAAAAt < 1:2895654/68‑1 (MQ=255) aaaGTGACCGGCATTGGACGCAACAGTATGCAGGGTGATATTCGCTTTGCTGATGTTCTGGAAAAAAt < 1:2162819/68‑1 (MQ=255) aaaGTGACCGGCATTGGACGCAACAGTATGCAGGGTGATATTCGCTTTGCTGATGTTCTGGAAAAAAt < 1:3200103/68‑1 (MQ=255) aaaGTGACCGGCATTGGACGCAACAGTATGCAGGGTGATATTCGCTTTGCTGATGTTCTGGAAAAAAt < 1:1534398/68‑1 (MQ=255) gTGACCGGTATTGGACGTAACAGTATGCAGGGTGATATTCGCTTTGCTGATGTGCTGGAAAAAATGGgc > 1:2239088/1‑69 (MQ=255) gTGACCGGTATTGGACGTAACAGTATGCAGGGTGATATTCGCTTTGCTGATGTGCTGGAAAAAATGGgc > 1:1710429/1‑69 (MQ=255) gTGACCGGTATTGGACGTAACAGTATGCAGGGTGATATTCGCTTTGCTGATGTGCTGGAAAAAATGGgc > 1:1682941/1‑69 (MQ=255) gTGACCGGTATTGGACGTAACAGTATGCAGGGTGATATTCGCTTTGCTGATGTGCTGGAAAAAATGGgc > 1:651175/1‑69 (MQ=255) gTGACCGGTATTGGACGTAACAGTATGCAGGGTGATATTCGCTTTGCTGATGTGCTGGAAAAAATGGgc > 1:2130049/1‑69 (MQ=255) gTGACCGGTATTGGACGTAACAGTATGCAGGGTGATATTCGCTTTGCTGATGTGCTGGAAAAAATGGgc > 1:1824442/1‑69 (MQ=255) | GCATCTTCGGCTTCTTACTTTCTGGCAGCAGCAGCAATCAAAGGCGGCACTGTAAAAGTGACCGGTATTGGACGTAACAGTATGCAGGGTGATATTCGCTTTGCTGATGTGCTGGAAAAAATGGGC > minE/616237‑616362 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |