| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 616,470 | G→C | 16.6% | T320T (ACG→ACC) | aroA → | 5‑enolpyruvylshikimate‑3‑phosphate synthetase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 616,470 | 0 | G | C | 16.6% | 136.3 / 18.2 | 66 | T320T (ACG→ACC) | aroA | 5‑enolpyruvylshikimate‑3‑phosphate synthetase |
| Reads supporting (aligned to +/- strand): ref base G (31/24); new base C (6/5); total (37/29) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.08e-01 | |||||||||||
GGTGAACTGAACGCTATTGATATGGATATGAACCATATTCCTGATGCGGCGATGACCATTGCCACGGCGGCGTTATTTGCAAAAGGCACCACCACGCTGCGCAATATCTATAACTGGCGTGTTA > minE/616405‑616528 | ggtgAACTGAACGCTATTGATATGGATATGAACCATATTCCTGATGCGGCGATGACCATTGCCAcggcg < 1:121094/69‑1 (MQ=255)ggtgAACTGAACGCTATTGATATGGATATGAACCATATTCCTGATGCGGCGATGACCATTGCCAcggcg < 1:2955133/69‑1 (MQ=255)ggtgAACTGAACGCTATTGATATGGATATGAACCATATTCCTGATGCGGCGATGACCATTGCCAcggcg < 1:2879556/69‑1 (MQ=255) gatatggatatgAACCATATTCCTGATGCGGCGATGACCATTGCCAcggc > 1:1705314/1‑50 (MQ=255) gatatggatatgAACCATATTCCTGATGCGGCGATGACCATTGCCACGGCGGCGTTATTTGCAAAAGGc > 1:2464912/1‑69 (MQ=255) gatatggatatgAACCATATTCCTGATGCGGCGATGACCATTGCCACGGCGGCGTTATTTGCAAAAGGc > 1:1248966/1‑69 (MQ=255) gatatggatatgAACCATATTCCTGATGCGGCGATGACCATTGCCACGGCGGCGTTATTTGCAAAAGGc > 1:3170025/1‑69 (MQ=255) gatatggatatgAACCATATTCCTGATGCGGCGATGACCATTGCCACGGCGGCGTTATTTGCAAAAGGc > 1:2551734/1‑69 (MQ=255) gatatggatatgAACCATATTCCTGATGCGGCGATGACCATTGCCACGGCGGCGTTATTTGCAAAAGGc > 1:2044445/1‑69 (MQ=255) gatatggatatgAACCATATTCCTGATGCGGCGATGACCATTGCCACGGCGGCGTTATTTGCAAAAGGc > 1:2820561/1‑69 (MQ=255) atggatatgAACCATATTCCTGATGCGGCGATGACCATTGCCACGGCGGCGTTATTTGCAAAAGGcac < 1:3211729/68‑1 (MQ=255) atggatatgAACCATATTCCTGATGCGGCGATGACCATTGCCACGGCGGCGTTATTTGCAAAAGGcac < 1:2015613/68‑1 (MQ=255) tggatatgAACCATATTCCTGATGCGGCGATGACCATTGCCACGGCGGCGTTATTTGCAAAAGGcacc > 1:2176677/1‑68 (MQ=255) atgAACCATATTCCTGATGCGGCGATGACCATTGCCACGGCGGCGTTATTTGCAAAAGGCACCACCACg > 1:2421223/1‑69 (MQ=255) atgAACCATATTCCTGATGCGGCGATGACCATTGCCACGGCGGCGTTATTTGCAAAAGGCACCACCACg > 1:2252865/1‑69 (MQ=255) atgAACCATATTCCTGATGCGGCGATGACCATTGCCACGGCGGCGTTATTTGCAAAAGGCACCACCACg > 1:2083545/1‑69 (MQ=255) atgAACCATATTCCTGATGCGGCGATGACCATTGCCACGGCGGCGTTATTTGCAAAAGGCACCACCACg > 1:3056668/1‑69 (MQ=255) atgAACCATATTCCTGATGCGGCGATGACCATTGCCACGGCGGCGTTATTTGCAAAAGGCACCACCACg > 1:3146337/1‑69 (MQ=255) atgAACCATATTCCTGATGCGGCGATGACCATTGCCACGGCGGCGTTATTTGCAAAAGGCACCACCACg > 1:3250854/1‑69 (MQ=255) cATATTCCTGATGCGGCGATGACCATTGCCACGGCGGCGTTATTTGCAAAAGGTACCACCACGCTgcgc < 1:2821153/69‑1 (MQ=255) cATATTCCTGATGCGGCGATGACCATTGCCACGGCGGCGTTATTTGCAAAAGGCACCACCACGCTgcgc < 1:2732468/69‑1 (MQ=255) cATATTCCTGATGCGGCGATGACCATTGCCACGGCGGCGTTATTTGCAAAAGGCACCACCACGCTgcgc < 1:2545286/69‑1 (MQ=255) cATATTCCTGATGCGGCGATGACCATTGCCACGGCGGCGTTATTTGCAAAAGGCACCACCACGCTgcgc < 1:463665/69‑1 (MQ=255) cATATTCCTGATGCGGCGATGACCATTGCCACGGCGGCGTTATTTGCAAAAGGCACCACCACGCTgcgc < 1:1107130/69‑1 (MQ=255) cATATTCCTGATGCGGCGATGACCATTGCCACGGCGGCGTTATTTGCAAAAGGCACCACCACGCTgcgc < 1:944106/69‑1 (MQ=255) cATATTCCTGATGCGGCGATGACCATTGCCACGGCGGCGTTATTTGCAAAAGGCACCACCACGCTgcgc < 1:1378985/69‑1 (MQ=255) cATATTCCTGATGCGGCGATGACCATTGCCACGGCGGCGTTATTTGCAAAAGGCACCACCACGCTgcgc < 1:1963344/69‑1 (MQ=255) ttCCTGATGCGGCGATGACCATTGCCACGGCGGCGTTATTTGCAAAAGGCACCACCACGCTGCGCAata > 1:2342102/1‑69 (MQ=255) ttCCTGATGCGGCGATGACCATTGCCACGGCGGCGTTATTTGCAAAAGGCACCACCACGCTGCGCAata > 1:367342/1‑69 (MQ=255) ttCCTGATGCGGCGATGACCATTGCCACGGCGGCGTTATTTGCAAAAGGCACCACCACGCTGCGCAata > 1:3063197/1‑69 (MQ=255) ttCCTGATGCGGCGATGACCATTGCCACGGCGGCGTTATTTGCAAAAGGCACCACCACGCTGCGCAata > 1:2117229/1‑69 (MQ=255) ccTGATGCGGCGATGACCATTGCCACGGCGGCGTTATTTGCAAAAGGcaccac < 1:12313/53‑1 (MQ=255) ccTGATGAGGCGATGACCATTGCCACGGCGGCGTTATTTGCAAAAGGcaccac < 1:2815959/53‑1 (MQ=255) tGATGCGGCGATGACCATTGCCACGGCGGCGTTATTTGCAAAAGGCACCACCACGCTGCGCAATATCt > 1:1950562/1‑68 (MQ=255) gATGCGGCGATGACCATTGCCACCGCGGCGTTATTTGCAAAAGGCACCACCACGCTGCGCAATATCtat < 1:3062553/69‑1 (MQ=255) gATGCGGCGATGACCATTGCCACCGCGGCGTTATTTGCAAAAGGCACCACCACGCTGCGCAATATCtat < 1:916225/69‑1 (MQ=255) gATGCGGCGATGACCATTGCCACCGCGGCGTTATTTGCAAAAGGCACCACCACGCTGCGCAATATCtat < 1:528475/69‑1 (MQ=255) gATGCGGCGATGACCATTGCCACCGCGGCGTTATTTGCAAAAGGCACCACCACGCTGCGCAATATCtat < 1:1410616/69‑1 (MQ=255) gATGCGGCGATGACCATTGCCACCGCGGCGTTATTTGCAAAAGGCACCACCACGCTGCGCAATATCtat < 1:1748455/69‑1 (MQ=255) gcggcgATGACCATTGCCACGGCGGCGTTATTTGCAAAAGGCACCACCACGCTGCGCAATATCtat < 1:2818598/66‑1 (MQ=255) gcggcgATGACCATTGCCACGGCGGCGTTATTTGCAAAAGGCACCACCACGCTGCGCAATATCtat < 1:2827039/66‑1 (MQ=255) gcggcgATGACCATTGCCACGGCGGCGTTATTTGCAAAAGGCACCACCACGCTGCGCAATATCtat < 1:2272168/66‑1 (MQ=255) gcggcgATGACCATTGCCACGGCGGCGTTATTTGCAAAAGGCACCACCACGCTGCGCAATATCtat < 1:723310/66‑1 (MQ=255) gcggcgATGACCATTGCCACGGCGGCGTTATTTGCAAAAGGCACCACCACGCTGCGCAATATCtat < 1:1747727/66‑1 (MQ=255) gcggcgATGACCATTGCCACGGCGGCGTTATTTGCAAAAGGCACCACCACGCTGCGCAATATCtat < 1:2422458/66‑1 (MQ=255) gcggcgATGACCATTGCCACGGCGGCGTTATTTGCAAAAGGCACCACCACGCTGCGCAATATCtat < 1:3231897/66‑1 (MQ=255) gcggcgATGACCATTGCCACCGCGGCGTTATTTGCAAAAgg > 1:3400283/1‑41 (MQ=255) gcggcgATGACCATTGCCACCGCGGCGTTATTTGCAAAAGGCACCACCACGCTGCGCAATATCTATAAc > 1:642147/1‑69 (MQ=255) gcggcgATGACCATTGCCACCGCGGCGTTATTTGCAAAAGGCACCACCACGCTGCGCAATATCTATAAc > 1:471756/1‑69 (MQ=255) gcggcgATGACCATTGCCACCGCGGCGTTATTTGCAAAAGGCACCACCACGCTGCGCAATATCTATAAc > 1:2503921/1‑69 (MQ=255) gcggcgATGACCATTGCCACCGCGGCGTTATTTGCAAAAGGCACCACCACGCTGCGCAATATCTATAAc > 1:314432/1‑69 (MQ=255) gcggcgATGACCATTGCCACCGCGGCGTTATTTGCAAAAGGCACCACCACGCTGCGCAATATCTATAAc > 1:3149538/1‑69 (MQ=255) cggcgATGACCATTGCCACGGCGGCGTTATTTGCAAAAGGCACCACCACGCTGCGCAATATCTATAACt > 1:2009193/1‑69 (MQ=255) cggcgATGACCATTGCCACGGCGGCGTTATTTGCAAAAGGCACCACCACGCTGCGCAATATCTATAACt > 1:869037/1‑69 (MQ=255) cggcgATGACCATTGCCACGGCGGCGTTATTTGCAAAAGGCACCACCACGCTGCGCAATATCTATAACt > 1:2474235/1‑69 (MQ=255) cggcgATGACCATTGCCACGGCGGCGTTATTTGCAAAAGGCACCACCACGCTGCGCAATATCTATAACt > 1:2474952/1‑69 (MQ=255) cggcgATGACCATTGCCACGGCGGCGTTATTTGCAAAAGGCACCACCACGCTGCGCAATATCTATAACt > 1:289628/1‑69 (MQ=255) cggcgATGACCATTGCCACGGCGGCGTTATTTGCAAAAGGCACCACCACGCTGCGCAATATCTATAACt > 1:1845961/1‑69 (MQ=255) aCCATTGCCACGGGGGCGTTATTTGCAAAAGGCACCACCACGCTGCGCAat > 1:3399239/1‑51 (MQ=255) aCCATTGCCACGGCGGCGTTATTTGCAAAAGGcaccaccac > 1:888024/1‑41 (MQ=255) aCCATTGCCACGGCGGCGTTATTTGCAAAAGGCACCACCACGCTGCGCAat > 1:1350443/1‑51 (MQ=255) aCCATTGCCACGGCGGCGTTATTTGCAAAAGGCACCACCACGCTGCGCAat > 1:1640915/1‑51 (MQ=255) aCCATTGCCACGGCGGCGTTATTTGCAAAAGGCACCACCACGCTGCGCAat > 1:226325/1‑51 (MQ=255) aCCATTGCCACGGCGGCGTTATTTGCAAAAGGCACCACCACGCTGCGCAat > 1:3062912/1‑51 (MQ=255) aCCATTGCCACGGCGGCGTTATTTGCAAAAGGCACCACAACGCTGCGCAat > 1:2972260/1‑51 (MQ=255) cATTGCCACGGCGGCGTTATTTGCAAAAGGCACCACCACGCTGCGCAATATCTATAACTGGCGTGTTa < 1:244656/68‑1 (MQ=255) cATTGCCACGGCGGCGTTATTTGCAAAAGGCACCACCACGCTGCGCAATATCTATAACTGGCGTGTTa < 1:2323208/68‑1 (MQ=255) cATTGCCACGGCGGCGTTATTTGCAAAAGGCACCACCACGCTGCGCAATATCTATAACTGGCGTGTTa < 1:2145564/68‑1 (MQ=255) cATTGCCACGGCGGCGTTATTTGCAAAAGGCACCACCACGCTGCGCAATATCTATAACTGGCGTGTTa < 1:780973/68‑1 (MQ=255) cATTGCCACGGCGGCGTTATTTGCAAAAGGCACCACCACGCTGCGCAATATCTATAACTGGCGTGTTa < 1:1195610/68‑1 (MQ=255) | GGTGAACTGAACGCTATTGATATGGATATGAACCATATTCCTGATGCGGCGATGACCATTGCCACGGCGGCGTTATTTGCAAAAGGCACCACCACGCTGCGCAATATCTATAACTGGCGTGTTA > minE/616405‑616528 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |