| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 617,160 | C→G | 41.6% | V66V (GTC→GTG) | ycaL → | predicted peptidase with chaperone function |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 617,160 | 0 | C | G | 41.6% | 55.8 / 107.0 | 108 | V66V (GTC→GTG) | ycaL | predicted peptidase with chaperone function |
| Reads supporting (aligned to +/- strand): ref base C (24/39); new base G (20/25); total (44/64) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 5.55e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
Only 100 of 116 total aligned reads displayed.
AAGCGATGCCGATGCAAAAGCGATTGCCAATCAGGGCTGTGCCGAAATGGACAGCGGCAATCAAGTCGCAAGTAAATCCAGCAAGTACGGTAAACGTCTGGCAAAAATCGCCAAAGCATTGGGTAACAATATT > minE/617094‑617226 | aaGCGATGCCGATGCAAAAGCGATTGCCAATCAGGGCTGTGCCGAAATGGACAGCGGCAATCACGTc > 1:2181198/1‑67 (MQ=255)aaGCGATGCCGATGCAAAAGCGATTGCCAATCAGGGCTGTGCCGAAATGGACAGCGGCAATCAAGTc > 1:2925828/1‑67 (MQ=255)aaGCGATGCCGATGCAAAAGCGATTGCCAATCAGGGCTGTGCCGAAATGGACAGCGGCAATCAAGTc > 1:3423876/1‑67 (MQ=255)aaGCGATGCCGATGCAAAAGCGATTGCCAATCAGGGCTGTGCCGAAATGGACAGCGGCAATCAAGTc > 1:2850908/1‑67 (MQ=255)aaGCGATGCCGATGCAAAAGCGATTGCCAATCAGGGCTGTGCCGAAATGGACAGCGGCAATCAAGTc > 1:2881211/1‑67 (MQ=255)aaGCGATGCCGATGCAAAAGCGATTGCCAATCAGGGCTGTGCCGAAATGGACAGCGGCAATCAAGTc > 1:1548175/1‑67 (MQ=255)aaGCGATGCCGAAGCAAAAGCGATTGCCAATCAGGGCTGTGCCGAAATGGACAGCGGCAATCAAGTc > 1:1348700/1‑67 (MQ=255) atgccgatgcAAAAGCGATTGCCAATCAGGGCTGTGCCGAAATGGACAGCGGCAATCAAGTCGCAAGt > 1:1094688/1‑68 (MQ=255) atgccgatgcAAAAGCGATTGCCAATCAGGGCTGTGCCGAAATGGACAGCGGCAATCAAGTCGCAAGTa > 1:703561/1‑69 (MQ=255) atgccgatgcAAAAGCGATTGCCAATCAGGGCTGTGCCGAAATGGACAGCGGCAATCAAGTCGCAAGTa > 1:2416231/1‑69 (MQ=255) atgccgatgcAAAAGCGATTGCCAATCAGGGCTGTGCCGAAATGGACAGCGGCAATCAAGTCGCAAGTa > 1:621951/1‑69 (MQ=255) atgccgatgcAAAAGCGATTGCCAATCAGGGCTGTGCCGAAATGGACAGCGGCAATCAAGTCGCAAGTa > 1:837714/1‑69 (MQ=255) atgccgatgcAAAAGCGATTGCCAATCAGGGCTGTGCCGAAATGGACAGCGGCAATCAAGTCGCAAGTa > 1:355874/1‑69 (MQ=255) atgccgatgcAAAAGCGATTGCCAATCAGGGCTGTGCCGAAATGGACAGCGGCAATCAAGTCGCAAGTa > 1:842147/1‑69 (MQ=255) atgccgatgcAAAAGCGATTGCCAATCAGGGCTGTGCCGAAATGGACAGCGGCAATCAAGTCGCAAGTa > 1:3059249/1‑69 (MQ=255) aaaGCGATTGCCAATCAGGGCTGTGCCGAAATGGACAGCGGCAATCAAGTCGCAAGTAAATCCAGCAAg < 1:1911010/69‑1 (MQ=255) ttGCCAATCAGGGCTGTGCCGAAATGGACAGCGGCAATCAAGTCGCAAGTAAATCCAGCAAGTACGGTa < 1:2086742/69‑1 (MQ=255) ttGCCAATCAGGGCTGTGCCGAAATGGACAGCGGCAATCAAGTCGCAAGTAAATCCAGCAAGTACGGTa < 1:1718614/69‑1 (MQ=255) ttGCCAATCAGGGCTGTGCCGAAATGGACAGCGGCAATCAAGTCGCAAGTAAATCCAGCAAGTACGGTa < 1:2439693/69‑1 (MQ=255) cAGGGCTGTGCCGAAATGGACAGCGGCAATCAAGTCGCAAGTAAATCCAGCAAGTACGGTAAACGTCt > 1:2756439/1‑68 (MQ=255) cAGGGCTGTGCCGAAATGGACAGCGGCAATCAAGTCGCAAGTAAATCCAGCAAGTACGGTAAACGTCt > 1:1598806/1‑68 (MQ=255) cAGGGCTGTGCCGAAATGGACAGCGGCAATCAAGTCGCAAGTAAATCCAGCAAGTACGGTAAACGTCt > 1:926612/1‑68 (MQ=255) cAGGGCTGTGCCGAAATGGACAGCGGCAATCAAGTCGCAAGTAAATCCAGCAAGTACGGTAAACGTCt > 1:1546802/1‑68 (MQ=255) cAGGGCTGTGCCGAAATGGACAGCGGCAATCAAGTCGCAAGTAAATCCAGCAAGTACGGTAAACGTCt > 1:1302792/1‑68 (MQ=255) cAGGGCTGTGCCGAAATGGACAGCGGCAATCAAGTCGCAAGTAAATCCAGCAAGTACGGTAAACGTCt > 1:609569/1‑68 (MQ=255) ggCTGTGTCGAAATGGACAGCGGCAATAAAGTGGCAAGCAAATCCAGCAAGTACGGGAAACGTCTGGCa < 1:2959851/69‑1 (MQ=255) ggCTGTGTCGAAATGGACAGCGGCAATAAAGTGGCAAGCAAATCCAGCAAGTACGGGAAACGTCTGGCa < 1:3014848/69‑1 (MQ=255) ggCTGTGTCGAAATGGACAGCGGCAATAAAGTGGCAAGCAAATCCAGCAAGTACGGGAAACGTCTGGCa < 1:2808051/69‑1 (MQ=255) ggCTGTGTCGAAATGGACAGCGGCAATAAAGTGGCAAGCAAATCCAGCAAGTACGGGAAACGTCTGGCa < 1:488109/69‑1 (MQ=255) ggCTGTGTCGAAATGGACAGCGGCAATAAAGTGGCAAGCAAATCCAGCAAGTACGGGAAACGTCTGGCa < 1:2736500/69‑1 (MQ=255) ggCTGTGTCGAAATGGACAGCGGCAATAAAGTGGCAAGCAAATCCAGCAAGTACGGGAAACGTCTGGCa < 1:689222/69‑1 (MQ=255) ggCTGTGTCGAAATGGACAGCGGCAATAAAGTGGCAAGCAAATCCAGCAAGTACGGGAAACGTCTGGCa < 1:724470/69‑1 (MQ=255) ggCTGTGTCGAAATGGACAGCGGCAATAAAGTGGCAAGCAAATCCAGCAAGTACGGGAAACGTCTGGCa < 1:877319/69‑1 (MQ=255) ggCTGTGTCGAAATGGACAGCGGCAATAAAGTGGCAAGCAAATCCAGCAAGTACGGGAAACGTCTGGCa < 1:2142873/69‑1 (MQ=255) ggCTGTGTCGAAATGGACAGCGGCAATAAAGTGGCAAGCAAATCCAGCAAGTACGGGAAACGTCTGGCa < 1:2354925/69‑1 (MQ=255) ggCTGTGTCGAAATGGACAGCGGCAATAAAGTGGCAAGCAAATCCAGCAAGTACGGGAAACGTCTGGCa < 1:1778318/69‑1 (MQ=255) ggCTGTGTCGAAATGGACAGCGGCAATAAAGTGGCAAGCAAATCCAGCAAGTACGGGAAACGTCTGGCa < 1:1288150/69‑1 (MQ=255) ggCTGTGTCGAAATGGACAGCGGCAATAAAGTGGCAAGCAAATCCAGCAAGTACGGGAAACGTCTGGCa < 1:132166/69‑1 (MQ=255) ggCTGTGTCGAAATGGACAGCGGCAATAAAGTGGCAAGCAAATCCAGCAAGTACGGGAAACGTCTGGCa < 1:1366708/69‑1 (MQ=255) ggCTGTGTCGAAATGGACAGCGGCAATAAAGTGGCAAGCAAATCCAGCAAGTACGGGAAACGTCTGGCa < 1:1157910/69‑1 (MQ=255) ggCTGTGTCGAAATGGACAGCGGCAATAAAGTGGCAAGCAAATCCAGCAAGTACGGGAAACGTCTGGCa < 1:1618834/69‑1 (MQ=255) ggCTGTGTCGAAATGGACAGCGGCAATAAAGTGGCAAGCAAATCCAGCAAGTACGGGAAACGTCTGGCa < 1:1088220/69‑1 (MQ=255) ggCTGTGTCGAAATGGACAGCGGCAATAAAGTGGCAAGCAAATCCAGCAAGTACGGGAAACGTCTGGCa < 1:190949/69‑1 (MQ=255) ggCTGTGTCGAAATGGACAGCGGCAATAAAGTGGCAAGCAAATCCAGCAAGTACGGGAAACGTCTGGCa < 1:1014010/69‑1 (MQ=255) gCTGTGTCCAAATGGACAGCGGCAATAAAGTGGCAAGCAAATCCAGCAAGTACGGGAAACGTCTGGCa < 1:2123430/68‑1 (MQ=255) tgtgCCGAAATGGACAGCGGCAATCAAGTCGCAAGTAAATCCAGCAAGTACGGTAAACGTCTGGCaaaa < 1:2074820/69‑1 (MQ=255) tgtgCCGAAATGGACAGCGGCAATCAAGTCGCAAGTAAATCCAGCAAGTACGGTAAACGTCTGGCaaaa < 1:3190178/69‑1 (MQ=255) tgtgCCGAAATGGACAGCGGCAATCAAGTCGCAAGTAAATCCAGCAAGTACGGTAAACGTCTGGCaaaa < 1:293428/69‑1 (MQ=255) gtgtCGAAATGGACAGCGGCAATAAAGTGGCAAGCAAATCCAGCAAGTACGGGAAACGTCTGGCa < 1:1628310/65‑1 (MQ=255) tgCCGAAATGGACAGCGGCAATCAAGTCGCAAGTAAATCCAGCAAGTACGGTAAACGTCTGGCaaaatt > 1:2481873/1‑67 (MQ=255) tgCCGAAATGGACAGCGGCAATCAAGTCGCAAGTAAATCCAGCAAGTACGGTAAACGTCTGGCAAAAAt > 1:901180/1‑69 (MQ=255) tgCCGAAATGGACAGCGGCAATCAAGTCGCAAGTAAATCCAGCAAGTACGGTAAACGTCTGGCAAAAAt > 1:3181157/1‑69 (MQ=255) tgCCGAAATGGACAGCGGCAATCAAGTCGCAAGTAAATCCAGCAAGTACGGTAAACGTCTGGCAAAAAt > 1:2606061/1‑69 (MQ=255) tcGATATGGACAGCGGCAATAAAGTGGCAAGCAAATCCAGCAAGTACGGGAAACGTCTGGCa < 1:891201/61‑1 (MQ=255) aaTGGACAGCGGCAATAAAGTGGCAATCAAATCCAGCAAGTACGGGAAACGTCTGGCAAAAATCGCCaa > 1:252159/1‑69 (MQ=255) aaTGGACAGCGGCAATAAAGTGGCAAGCAAATCCAGCAAGTACg > 1:2275917/1‑44 (MQ=38) aaTGGACAGCGGCAATAAAGTGGCAAGCAAATCCAGCAAGTACGGGAAACGTCTgg > 1:2272110/1‑56 (MQ=255) aaTGGACAGCGGCAATAAAGTGGCAAGCAAATCCAGCAAGTACGGGAAACGTCTGGCAAAAATc > 1:3112079/1‑64 (MQ=255) aaTGGACAGCGGCAATAAAGTGGCAAGCAAATCCAGCAAGTACGGGAAACGTCTGGCAAAAATCGCCaa > 1:354971/1‑69 (MQ=255) aaTGGACAGCGGCAATAAAGTGGCAAGCAAATCCAGCAAGTACGGGAAACGTCTGGCAAAAATCGCCaa > 1:3131665/1‑69 (MQ=255) aaTGGACAGCGGCAATAAAGTGGCAAGCAAATCCAGCAAGTACGGGAAACGTCTGGCAAAAATCGCCaa > 1:3078932/1‑69 (MQ=255) aaTGGACAGCGGCAATAAAGTGGCAAGCAAATCCAGCAAGTACGGGAAACGTCTGGCAAAAATCGCCaa > 1:1346211/1‑69 (MQ=255) aaTGGACAGCGGCAATAAAGTGGCAAGCAAATCCAGCAAGTACGGGAAACGTCTGGCAAAAATCGCCaa > 1:2108146/1‑69 (MQ=255) aaTGGACAGCGGCAATAAAGTGGCAAGCAAATCCAGCAAGTACGGGAAACGTCTGGCAAAAATCGCCaa > 1:2294459/1‑69 (MQ=255) aaTGGACAGCGGCAATAAAGTGGCAAGCAAATCCAGCAAGTACGGGAAACGTCTGGCAAAAATCGCCaa > 1:2995445/1‑69 (MQ=255) aaTGGACAGCGGCAATAAAGTGGCAAGCAAATCCAGCAAGTACGGGAAACGTCTGGCAAAAATCGCCaa > 1:2226952/1‑69 (MQ=255) aaTGGACAGCGGCAATAAAGTGGCAAGCAAATCCAGCAAGTACGGGAAACGTCTGGCAAAAATCGCCaa > 1:2773926/1‑69 (MQ=255) aaTGGACAGCGGCAATAAAGTGGCAAGCAAATCCAGCAAGTACGGGAAACGTCTGGCAAAAATCGCCaa > 1:815124/1‑69 (MQ=255) aaTGGACAGCGGCAATAAAGTGGCAAGCAAATCCAGCAAGTACGGGAAACGTCTGGCAAAAATCGCCaa > 1:2182558/1‑69 (MQ=255) aaTGGACAGCGGCAATAAAGTGGCAAGCAAATCCAGCAAGTACGGGAAACGTCTGGCAAAAATCGCCaa > 1:1781174/1‑69 (MQ=255) aaTGGACAGCGGCAATAAAGTGGCAAGCAAATCCAGCAAGTACGGGAAACGTCTGGCAAAAATCGCCaa > 1:2024753/1‑69 (MQ=255) aaTGGACAGCGGCAATAAAGTGGCAAGCAAATCCAGCAAGTACGGGAAACGTCTGGCAAAAATCGCCaa > 1:1082924/1‑69 (MQ=255) ggACAGCGGCAATCAAGTCGCAAGTAAATCCAGCAAGTACGGTAAACGTCTGGCaaaa < 1:3391805/58‑1 (MQ=255) ggACAGCGGCAATCAAGTCGCAAGTAAATCCAGCAAGTACGGTAAACGTCTGGCAAAAATCGCCAAAGc < 1:1011112/69‑1 (MQ=255) ggACAGCGGCAATCAAGTCGCAAGTAAATCCAGCAAGTACGGTAAACGTCTGGCAAAAATCGCCAAAGc < 1:1193835/69‑1 (MQ=255) ggACAGCGGCAATCAAGTCGCAAGTAAATCCAGCAAGTACGGTAAACGTCTGGCAAAAATCGCCAAAGc < 1:2045515/69‑1 (MQ=255) ggACAGCGGCAATCAAGTCGCAAGTAAATCCAGCAAGTACGGTAAACGTCTGGCAAAAATCGCCAAAGc < 1:1106539/69‑1 (MQ=255) ggACAGCGGCAATCAAGTCGCAAGTAAATCCAGCAAGTACGGTAAACGTCTGGCAAAAATCGCCAAAGc < 1:302321/69‑1 (MQ=255) ggACAGCGGCAATCAAGTCGCAAGTAAATCCAGCAAGTACGGTAAACGTCTGGCAAAAATCGCCAAAGc < 1:3038985/69‑1 (MQ=255) ggACAGCGGCAATCAAGTCGCAAGTAAATCCAGCAAGTACGGTAAACGTCTGGCAAAAATCGCCAAAGc < 1:2445483/69‑1 (MQ=255) ggACAGCGGCAATCAAGTCGCAAGTAAATCCAGCAAGTACGGTAAACGTCTGGCAAAAATCGCCAAAGc < 1:523912/69‑1 (MQ=255) tCAAGTCGCAAGTAAATCCAGCAAGTACGGTAAACGTCTGGCAAAAATCGCCAAAGCATTGGGTAACaa < 1:2084587/69‑1 (MQ=255) tCAAGTCGCAAGTAAATCCAGCAAGTACGGTAAACGTCTGGCAAAAATCGCCAAAGCATTGGGTAACaa < 1:1025832/69‑1 (MQ=255) tCAAGTCGCAAGTAAATCCAGCAAGTACGGTAAACGTCTGGCAAAAATCGCCAAAGCATTGGGTAACaa < 1:1190221/69‑1 (MQ=255) tCAAGTCGCAAGTAAATCCAGCAAGTACGGTAAACGTCTGGCAAAAATCGCCAAAGCATTGGGTAACaa < 1:1319462/69‑1 (MQ=255) tCAAGTCGCAAGTAAATCCAGCAAGTACGGTAAACGTCTGGCAAAAATCGCCAAAGCATTGGGTAACaa < 1:3352461/69‑1 (MQ=255) tCAAGTCGCAAGTAAATCCAGCAAGTACGGTAAACGTCTGGCAAAAATCGCCAAAGCATTGGGTAACaa < 1:1403169/69‑1 (MQ=255) tCAAGTCGCAAGTAAATCCAGCAAGTACGGTAAACGTCTGGCAAAAATCGCCAAAGCATTGGGTAACaa < 1:2671760/69‑1 (MQ=255) tCAAGTCGCAAGTAAATCCAGCAAGTACGGTAAACGTCTGGCAAAAATCGCCAAAGCATTGGGTAACaa < 1:2278056/69‑1 (MQ=255) tCAAGTCGCAAGTAAATCCAGCAAGTACGGTAAACGTCTGGCAAAAATCGCCAAAGCATTGGGTAACaa < 1:2259864/69‑1 (MQ=255) tCAAGTCGCAAGTAAATCCAGCAAGTACGGTAAACGTCTGGCAAAAATCGCCAAAGCATTGGGTAACaa < 1:2205516/69‑1 (MQ=255) tCAAGTCGCAAGTAAATCCAGCAAGTACGGTAAACGTCTGGCAAAAATCGCCAAAGCATTGGGTAACaa < 1:2063861/69‑1 (MQ=255) cAAGTCGCAAGTAAATCCAGCAAGTACGGTAAACGTCTGGCAAAAATCGCCAAAGCATTGGGTAACaa > 1:75086/1‑68 (MQ=255) cAAGTCGCAAGTAAATCCAGCAAGTACGGTAAACGTCTGGCAAAAATCGCCAAAGCATTGGGTAACaa > 1:1811227/1‑68 (MQ=255) aGTCGCAAGTAAATCCAGCAAGTACGGTAAACGTCTGGCAAAAATCGCCAAAGCATTGGGTAACAata > 1:1166095/1‑68 (MQ=255) gTCGCAAGTAAATCCAGCAAGTACGGTAAACGTCTGGCAAAAATCGCCAAAGCATTGGGTAACAATAtt < 1:295779/69‑1 (MQ=255) gTCGCAAGTAAATCCAGCAAGTACGGTAAACGTCTGGCAAAAATCGCCAAAGCATTGGGTAACAATAtt < 1:1580644/69‑1 (MQ=255) gTCGCAAGTAAATCCAGCAAGTACGGTAAACGTCTGGCAAAAATCGCCAAAGCATTGGGTAACAATAtt < 1:805149/69‑1 (MQ=255) gTCGCAAGTAAATCCAGCAAGTACGGTAAACGTCTGGCAAAAATCGCCAAAGCATTGGGTAACAATAtt < 1:2773910/69‑1 (MQ=255) tCGCAAGTAAATCCAGCAAGTACGGTAAACGTCTGGCAAAAATCGCCAAAGCATTGGGTAACAATAtt < 1:1757289/68‑1 (MQ=255) | AAGCGATGCCGATGCAAAAGCGATTGCCAATCAGGGCTGTGCCGAAATGGACAGCGGCAATCAAGTCGCAAGTAAATCCAGCAAGTACGGTAAACGTCTGGCAAAAATCGCCAAAGCATTGGGTAACAATATT > minE/617094‑617226 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |