| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 68,518 | C→A | 65.6% | intergenic (‑70/‑590) | leuL ← / → leuO | leu operon leader peptide/DNA‑binding transcriptional activator |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 68,518 | 0 | C | A | 65.6% | 42.8 / 47.9 | 61 | intergenic (‑70/‑590) | leuL/leuO | leu operon leader peptide/DNA‑binding transcriptional activator |
| Reads supporting (aligned to +/- strand): ref base C (17/4); new base A (22/18); total (39/22) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 5.42e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
GATGAATGCGATATGCTTTTAGAGTTACTGGATACAAAAACGGATGTCAACCCTGACGCAATAAAAACGTCCCGCCAGCGTGAGTTCTGCATCCGTAAAATTAGCTAATTGTGCT > minE/68462‑68576 | gATGAATGCGATATGCTTTTAGAGTTACTGGATACAAAAACGGATGTCAACCCTGAAGCAATAAAAACg < 1:1839251/69‑1 (MQ=255)gATGAATGCGATATGCTTTTAGAGTTACTGGATACAAAAACGGATGTCAACCCTGAAGCAATAAAAACg < 1:58748/69‑1 (MQ=255)gATGAATGCGATATGCTTTTAGAGTTACTGGATACAAAAACGGATGTCAACCCTGAAGCAATAAAAACg < 1:2820995/69‑1 (MQ=255)gATGAATGCGATATGCTTTTAGAGTTACTGGATACAAAAACGGATGTCAACCCTGAAGCAATAAAAACg < 1:2385522/69‑1 (MQ=255)gATGAATGCGATATGCTTTTAGAGTTACTGGATACAAAAACGGATGTCAACCCTGAAGCAATAAAAACg < 1:2102740/69‑1 (MQ=255)gATGAATGCGATATGCTTTTAGAGTTACTGGATACAAAAACGGATGTCAACCCTGAAGCAATAAAAACg < 1:295422/69‑1 (MQ=255)gATGAATGCGATATGCTTTTAGAGTTACTGGATACAAAAACGGATGTCAACCCTGAAGCAATAAAAACg < 1:2519351/69‑1 (MQ=255)gATGAATGCGATATGCTTTTAGAGTTACTGGATACAAAAACGGATGTCAACCCTGAAGCAATAAAAACg < 1:2785647/69‑1 (MQ=255)gATGAATGCGATATGCTTTTAGAGTTACTGGATACAAAAACGGATGTCAACCCTGAAGCAATAAAAACg < 1:1809682/69‑1 (MQ=255)gATGAATGCGATATGCTTTTAGAGTTACTGGATACAAAAACGGATGTCAACCCTGAAGCAATAAAAACg < 1:2757324/69‑1 (MQ=255)gATGAATGCGATATGCTTTTAGAGTTACTGGATACAAAAACGGATGTCAACCCTGAAGCAATAAAAACg < 1:176523/69‑1 (MQ=255)gATGAATGCGATATGCTTTTAGAGTTACTGGATACAAAAACGGATGTCAACCCTGAAGCAATAAAAACg < 1:912879/69‑1 (MQ=255)gATGAATGCGATATGCTTTTAGAGTTACTGGATACAAAAACGGATGTCAACCCTGAAGCAATAAAAACg < 1:915301/69‑1 (MQ=255)gATGAATGCGATATGCTTTTAGAGTTACTGGATACAAAAACGGATGTCAACCCTGAAGCAATAAAAACg < 1:1485148/69‑1 (MQ=255)gATGAATGCGATATGCTTTTAGAGTTACTGGATACAAAAACGGATGTCAACCCTGAAGCAATAAAAACg < 1:1428900/69‑1 (MQ=255)gATGAATGCGATATGCTTTTAGAGTTACTGGATACAAAAACGGATGTCAACCCTGAAGCAATAAAAACg < 1:3196556/69‑1 (MQ=255)gATGAATGCGATATGCTTTTAGAGTTACTGGATACAAAAACGGATGTCAACCCTGAAGCAATAAAAACg < 1:3340726/69‑1 (MQ=255) aTGAATGCGATATGCTTTTAGAGTTACTGGATACAAAAACGGATGTCAACCCTGAAGCAATAAAAACg < 1:2144026/68‑1 (MQ=255) tGCGATATGCTTTTAGAGTTACTGGATACAAAAACGGATGTCACCCCTGACGCAAAAACAACGTcc > 1:910699/1‑66 (MQ=255) tGCGATATGCTTTTAGAGTTACTGGATACAAAAACGGATGTCAACCCTGACGCAATAAAAACGTccc > 1:1917701/1‑67 (MQ=255) atatGCTTTTAGAGTTACTGGATACAAAAACGGATGTCAACCCTGACGCAATAAAAACg > 1:2796199/1‑59 (MQ=255) atGCTTTTAGAGTTACTGGATACAAAAACGGATGTCAACCCTGACGCAATAAAAACGTCCCGCCAg > 1:74277/1‑66 (MQ=255) atGCTTTTAGAGTTACTGGATACAAAAACGGATGTCAACCCTGACGCAATAAAAACGTCCCGCCAGc > 1:2621426/1‑67 (MQ=255) atGCTTTTAGAGTTACTGGATACAAAAACGGATGTCAACCCTGACGCAATAAAAACGTCCCGCCAGc > 1:2986334/1‑67 (MQ=255) atGCTTTTAGAGTTACTGGATACAAAAACGGATGTCAACCCTGACGCAATAAAAACGTCCCGCCAGc > 1:3376985/1‑67 (MQ=255) atGCTTTTAGAGTTACTGGATACAAAAACGGATGTCAACCCTGACGCAATAAAAACGTCCCGCCAGc > 1:900105/1‑67 (MQ=255) tGCTTTTAGAGTTACTGGATACAAAAACGGATGTCAACCCTGACGCAATaaa < 1:1079055/52‑1 (MQ=255) tGCTTTTAGAGTTACTGGATACAAAAACGGATGTCAACCCTGACGCAATaaa > 1:542971/1‑52 (MQ=255) tGCTTTTAGAGTTACTGGATACAAAAACGGATGTCAACCCTGACGCAATaaa > 1:717052/1‑52 (MQ=255) tGCTTTTAGAGTTACTGGATACAAAAACGGATGTCAACCCTGACGCAATaaa > 1:1154076/1‑52 (MQ=255) tGCTTTTAGAGTTACTGGATACAAAAACGGATGTCAACCCTGACGCAATAAAAACGTCCCGCCAGCGTg < 1:486815/69‑1 (MQ=255) gTTACTGGATACAAAAACGGATGTCAACCCTGACGCAATAAAAACGTCCCGCCAGCGTGAGTTCTGCAt > 1:801652/1‑69 (MQ=255) gTTACTGGATACAAAAACGGATGTCAACCCTGACGCAATAAAAACGTCCCGCCAGCGTGAGTTCTGCAt > 1:303947/1‑69 (MQ=255) gTTACTGGATACAAAAACGGATGTCAACCCTGACGCAATAAAAACGTCCCGCCAGCGTGAGTTCTGCAt > 1:3342424/1‑69 (MQ=255) ttACTGGATACAAAAACGGATGTCAACCCTGACGCAATAAAAACGTCCCGCCAGCGTGAGTTCTGCATc > 1:2655027/1‑69 (MQ=255) gATACAAAAACGGATGTCAACCCTGAAGCAATAAAAACGTCACGCCat > 1:3105531/1‑47 (MQ=255) gATACAAAAACGGATGTCAACCCTGAAGCAATAAAAACGTCACGCCATTGTGAGTTCGGCATCCGTaaa > 1:497358/1‑69 (MQ=255) gATACAAAAACGGATGTCAACCCTGAAGCAATAAAAACGTCACGCCATTGTGAGTTCGGCATCCGTaaa > 1:63770/1‑69 (MQ=255) gATACAAAAACGGATGTCAACCCTGAAGCAATAAAAACGTCACGCCATTGTGAGTTCGGCATCCGTaaa > 1:36818/1‑69 (MQ=255) gATACAAAAACGGATGTCAACCCTGAAGCAATAAAAACGTCACGCCATTGTGAGTTCGGCATCCGTaaa > 1:800151/1‑69 (MQ=255) gATACAAAAACGGATGTCAACCCTGAAGCAATAAAAACGTCACGCCATTGTGAGTTCGGCATCCGTaaa > 1:868393/1‑69 (MQ=255) gATACAAAAACGGATGTCAACCCTGAAGCAATAAAAACGTCACGCCATTGTGAGTTCGGCATCCGTaaa > 1:982330/1‑69 (MQ=255) gATACAAAAACGGATGTCAACCCTGAAGCAATAAAAACGTCACGCCATTGTGAGTTCGGCATCCGTaaa > 1:3132536/1‑69 (MQ=255) gATACAAAAACGGATGTCAACCCTGAAGCAATAAAAACGTCACGCCATTGTGAGTTCGGCATCCGTaaa > 1:1598495/1‑69 (MQ=255) gATACAAAAACGGATGTCAACCCTGAAGCAATAAAAACGTCACGCCATTGTGAGTTCGGCATCCGTaaa > 1:1721695/1‑69 (MQ=255) gATACAAAAACGGATGTCAACCCTGAAGCAATAAAAACGTCACGCCATTGTGAGTTCGGCATCCGTaaa > 1:1852527/1‑69 (MQ=255) gATACAAAAACGGATGTCAACCCTGAAGCAATAAAAACGTCACGCCATTGTGAGTTCGGCATCCGTaaa > 1:1884093/1‑69 (MQ=255) gATACAAAAACGGATGTCAACCCTGAAGCAATAAAAACGTCACGCCATTGTGAGTTCGGCATCCGTaaa > 1:2449580/1‑69 (MQ=255) gATACAAAAACGGATGTCAACCCTGAAGCAATAAAAACGTCACGCCATTGTGAGTTCGGCATCCGTaaa > 1:2461862/1‑69 (MQ=255) gATACAAAAACGGATGTCAACCCTGAAGCAATAAAAACGTCACGCCATTGTGAGTTCGGCATCCGTaaa > 1:2476925/1‑69 (MQ=255) gATACAAAAACGGATGTCAACCCTGAAGCAATAAAAACGTCACGCCATTGTGAGTTCGGCATCCGTaaa > 1:2557701/1‑69 (MQ=255) gATACAAAAACGGATGTCAACCCTGAAGCAATAAAAACGTCACGCCATTGTGAGTTCGGCATCCGTaaa > 1:2642834/1‑69 (MQ=255) gATACAAAAACGGATGTCAACCCTGAAGCAATAAAAACGTCACGCCATTGTGAGTTCGGCATCCGTaaa > 1:2842997/1‑69 (MQ=255) gATACAAAAACGGATGTCAACCCTGAAGCAATAAAAACGTCACGCCATTGTGAGTTCGGCATCCGTaaa > 1:3132557/1‑69 (MQ=255) gATACAAAAACGGATGTCAACCCTGAAGCAATAAAAACGTCACGCCATTGTGAGTTCGGCATCCGTaa > 1:1080790/1‑68 (MQ=255) gATACAAAAACGGATGTCAACCCTGAAGCAATAAAAACGTCACGCCATTGCGAGTTCGGCATCCGTaaa > 1:1744236/1‑69 (MQ=255) gATACAAAAACGGATGTCAACCCTGAAGCAAGAAAAACGTCACGCCATTGTGAGTTCGGCATCCGTaaa > 1:1777870/1‑69 (MQ=255) aaaCGGATGTCAACCCTGACGCAATAAAAACGTCCCGCCAGCGTGAGTTCTGCATCCGTAAAATTAGCt < 1:2607044/69‑1 (MQ=255) aaaCGGATGTCAACCCTGACGCAATAAAAACGTCCCGCCAGCGTGAGTTCTGCATCCGTAAAATTAGCt < 1:187118/69‑1 (MQ=255) tCAACCCTGACGCAATAAAAACGTCCCGCCAGCGTGAGTTCTGCATCCGTAAAATTAGCTAATTGtgct > 1:71136/1‑69 (MQ=255) tCAACCCTGACGCAATAAAAACGTCCCGCCAGCGTGAGTTCTGCATCCGTAAAATTAGCTAATTGtgct > 1:1875003/1‑69 (MQ=255) | GATGAATGCGATATGCTTTTAGAGTTACTGGATACAAAAACGGATGTCAACCCTGACGCAATAAAAACGTCCCGCCAGCGTGAGTTCTGCATCCGTAAAATTAGCTAATTGTGCT > minE/68462‑68576 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |