Predicted mutation
evidence seq id position mutation freq annotation gene description
RA minE 622,463 G→T 28.5% G482V (GGA→GTA)  ycaI → conserved inner membrane protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*minE622,4630GT28.5% 198.3 / 73.4 133G482V (GGA→GTA) ycaIconserved inner membrane protein
Reads supporting (aligned to +/- strand):  ref base G (48/47);  new base T (16/22);  total (64/69)
Fisher's exact test for biased strand distribution p-value = 4.44e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 139 total aligned reads displayed.

GTTCTTACTGGTGGTATGGCGATTAAACGCCTGGCGAACGTTGCCAGCAATGTGTGTGGCTGGAGGCTTGCTGATGTGCTGGCCGCTGTGGCAAAAACCTCGACCTGACGAGTGGCAGCTGTACATGCTT  >  minE/622401‑622530
                                                              |                                                                   
gTTCTTACTGGTGGTATGGCGATTAAACGCCTGGCGAACGTTGCCAGCAATGTGTGTGGCTGGAGGCtt                                                               >  1:2419745/1‑69 (MQ=255)
gTTCTTACTGGTGGTATGGCGATTAAACGCCTGGCGAACGTTGCCAGCAATGTGTGTGGCTGGAGGCtt                                                               >  1:1005050/1‑69 (MQ=255)
gTTCTTACTGGTGGTATGGCGATTAAACGCCTGGCGAACGTTGCCAGCAATGTGTGTGGCTGGAGGCtt                                                               >  1:1410223/1‑69 (MQ=255)
gTTCTTACTGGTGGTATGGCGATTAAACGCCTGGCGAACGTTGCCAGCAATGTGTGTGGCTGGAGGCtt                                                               >  1:2615576/1‑69 (MQ=255)
gTTCTTACTGGTGGTATGGCGATTAAACGCCTGGCGAACGTTGCCAGCAATGTGTGTGGCTGGAGGCtt                                                               >  1:498854/1‑69 (MQ=255)
gTTCTTACTGGTGGTATGGCGATTAAACGCCTGGCGAACGTTGCCAGCAATGTGTGTGGCTGGAGGCtt                                                               >  1:1776972/1‑69 (MQ=255)
gTTCTTACTGGTGGTATGGCGATTAAACGCCTGGCGAACGTTGCCAGCAATGTGTGTGGCTGGAGGCtt                                                               >  1:3342620/1‑69 (MQ=255)
  tCTTACTGGTGGTATGGCGATTAAACGCCTGGCGAACGTTGCCAGCAATGTGTGTGGCTGGAGGCTTGc                                                             <  1:200367/69‑1 (MQ=255)
   cTTACTGGTGGTATGGCGATTAAACGCCTGGCGAACGTTGCCAGCAATGTGTGTGGCTGGAGGCTTGCt                                                            <  1:3407717/69‑1 (MQ=255)
   cTTACTGGTGGTATGGCGATTAAACGCCTGGCGAACGTTGCCAGCAATGTGTGTGGCTGGAGGCTTGCt                                                            <  1:1655379/69‑1 (MQ=255)
   cTTACTGGTGGTATGGCGATTAAACGCCTGGCGAACGTTGCCAGCAATGTGTGTGGCTGGAGGCTTGCt                                                            <  1:154059/69‑1 (MQ=255)
   cTTACTGGTGGTATGGCGATTAAACGCCTGGCGAACGTTGCCAGCAATGTGTGTGGCTGGAGGCTTGCt                                                            <  1:3365769/69‑1 (MQ=255)
   cTTACTGGTGGTATGGCGATTAAACGCCTGGCGAACGTTGCCAGCAATGTGTGTGGCTGGAGGCTTGCt                                                            <  1:3133832/69‑1 (MQ=255)
   cTTACTGGTGGTATGGCGATTAAACGCCTGGCGAACGTTGCCAGCAATGTGTGTGGCTGGAGGCTTGCt                                                            <  1:2015411/69‑1 (MQ=255)
   cTTACTGGTGGTATGGCGATTAAACGCCTGGCGAACGTTGCCAGCAATGTGTGTGGCTGGAGGCTTGCt                                                            <  1:2133259/69‑1 (MQ=255)
   cTTACTGGTGGTATGGCGATTAAACGCCTGGCGAACGTTGCCAGCAATGTGTGTGGCTGGAGGCTTGCt                                                            <  1:2578157/69‑1 (MQ=255)
   cTTACTGGTGGTATGGCGATTAAACGCCTGGCGAACGTTGCCAGCAATGTGTGTGGCTGGAGGCTTGCt                                                            <  1:2350881/69‑1 (MQ=255)
   cTTACTGGTGGTATGGCGATTAAACGCCTGGCGAACGTTGCCAGCAATGTGTGTGGCTGGAGGCTTGCt                                                            <  1:788905/69‑1 (MQ=255)
   cTTACTGGTGGTATGGCGATTAAACGCCTGGCGAACGTTGCCAGCAATGTGTGTGGCTGGAGGCTTGCt                                                            <  1:1171048/69‑1 (MQ=255)
   cTTACTGGTGGTATGGCGATTAAACGCCTGGCGAACGTTGCCAGCAATGTGTGTGGCTGGAGGCTTGCt                                                            <  1:1050494/69‑1 (MQ=255)
   cTTACTGGTGGTATGGCGATTAAACGCCTGGCGAACGTTGCCAGCAATGTGTGTGGCTGGAGGCTTGCt                                                            <  1:1197725/69‑1 (MQ=255)
   cTTACTGGTGGTATGGCGATTAAACGCCTGGCGAACGTTGCCAGCAATGTGTGTGGCTGGAGGCTTGCt                                                            <  1:1263264/69‑1 (MQ=255)
   cTTACTGGTGGTATGGCGATTAAACGCCTGGCGAACGTTGCCAGCAATGTGTGTGGCTGGAGGCTAGCt                                                            <  1:132796/69‑1 (MQ=255)
   cTTACTGGTGGTATGGCGATTAAACGCCTGGCGAACGTTGCCAGCAATGTGTGTGGCTGGAGGCTAGCt                                                            <  1:128800/69‑1 (MQ=255)
   cTTACTGGTGGTATGGCGATTAAACGCCTGGCGAACGTTGCCAGCAATGTGTGTGGCTGGAGCCTTGCt                                                            <  1:2202/69‑1 (MQ=255)
                  gCGATTAAACGCCTTGCGAACGTTGCCAGCAATGTGTGTGGCTGGAGGCTTGCTGATGTGCTGGCCGCt                                             >  1:2610640/1‑69 (MQ=255)
                  gCGATTAAACGCCTGGCGAACGTTGCCAGCAATGTGTGTGGCTGTAGGCTTGCTGATGTGCTTGCCGCt                                             >  1:3167335/1‑69 (MQ=255)
                  gCGATTAAACGCCTGGCGAACGTTGCCAGCAATGTGTGTGGCTGTAGGCTTGCTGATGTGCTTGCCGCt                                             >  1:2059364/1‑69 (MQ=255)
                  gCGATTAAACGCCTGGCGAACGTTGCCAGCAATGTGTGTGGCTGTAGGCTTGCTGATGTGCTTGCCGCt                                             >  1:720776/1‑69 (MQ=255)
                  gCGATTAAACGCCTGGCGAACGTTGCCAGCAATGTGTGTGGCTGTAGGCTTGCTGATGTGCTTGCCGCt                                             >  1:2226493/1‑69 (MQ=255)
                  gCGATTAAACGCCTGGCGAACGTTGCCAGCAATGTGTGTGGCTGTAGGCTTGCTGATGTGCTTGCCGCt                                             >  1:3188091/1‑69 (MQ=255)
                  gCGATTAAACGCCTGGCGAACGTTGCCAGCAATGTGTGTGGCTGTAGGCTTGCTGATGTGCTTGCCGCt                                             >  1:1800434/1‑69 (MQ=255)
                  gCGATTAAACGCCTGGCGAACGTTGCCAGCAATGTGTGTGGCTGTAGGCTTGCTGATGTGCTTGCCGCt                                             >  1:2526068/1‑69 (MQ=255)
                  gCGATTAAACGCCTGGCGAACGTTGCCAGCAATGTGTGTGGCTGTAGGCTTGCTGATGTGCTTGCCGCt                                             >  1:705602/1‑69 (MQ=255)
                  gCGATTAAACGCCTGGCGAACGTTGCCAGCAATGTGTGTGGCTGTAGGCTTGCTGATGTGCTTGCCGCt                                             >  1:3401510/1‑69 (MQ=255)
                  gCGATTAAACGCCTGGCGAACGTTGCCAGCAATGTGTGTGGCTGTAGGCTTGCTGATGTGCTTGCCGCt                                             >  1:1026004/1‑69 (MQ=255)
                  gCGATTAAACGCCTGGCGAACGTTGCCAGCAATGTGTGTGGCTGGAGGCTTGc                                                             >  1:1303056/1‑53 (MQ=255)
                  gCGATTAAACGCCTGGCGAACGTTGCCAGCAATGTGTGTGGCTGGAGGCTTGCt                                                            >  1:2562755/1‑54 (MQ=255)
                  gCGATTAAACGCCTGGCGAACGTTGCCAGCAATGTGTGTGGCTGGAGGCTTGCt                                                            >  1:2897898/1‑54 (MQ=255)
                  gCGATTAAACGCCTGGCGAACGTTGCCAGCAATGTGTGTGGCTGGAGGCTTGCt                                                            >  1:906129/1‑54 (MQ=255)
                  gCGATTAAACGCCTGGCGAACGTTGCCAGCAATGTGTGTGGCTGGAGGCTTGCt                                                            >  1:2642133/1‑54 (MQ=255)
                  gCGATTAAACGCCTGGCGAACGTTGCCAGCAATGTGTGTGGCTGGAGGCTTGCt                                                            >  1:3424572/1‑54 (MQ=255)
                  gCGATTAAACGCCTGGCGAACGTTGCCAGCAATGTGTGTGGCTGGAGGCTTGCt                                                            >  1:2941185/1‑54 (MQ=255)
                  gCGATTAAACGCCTGGCGAACGTTGCCAGCAATGTGTGTGGCTGGAGGCTTGCt                                                            >  1:3297770/1‑54 (MQ=255)
                  gCGATTAAACGCCTGGCGAACGTTGCCAGCAATGTGTGTGGCTGGAGGCTTGCt                                                            >  1:913665/1‑54 (MQ=255)
                  gCGATTAAACGCCTGGCGAACGTTGCCAGCAATGTGTGTGGCTGGAGGCTTGCt                                                            >  1:340407/1‑54 (MQ=255)
                  gCGATTAAACGCCTGGCGAACGTTGCCAGCAATGTGTGTGGCTGGAGGCTTGCt                                                            >  1:1843875/1‑54 (MQ=255)
                  gCGATTAAACGCCTGGCGAACGTTGCCAGCAATGTGTGTGGCTGGAGGCTTGCt                                                            >  1:2182270/1‑54 (MQ=255)
                  gCGATTAAACGCCTGGCGAACGTTGCCAGCAATGTGTGTGGCTGGAGGCTTGCt                                                            >  1:2063330/1‑54 (MQ=255)
                  gCGATTAAACGCCTGGCGAACGTTGCCAGCAATGTGTGTGGCTGGAGGCTTGCTGATGTGCTGGCCGc                                              <  1:36000/68‑1 (MQ=255)
                  gCGATTAAACGCCTGGCGAACGTTGCCAGCAATGTGTGTGGCTGGAGGCTTGCTGATGTGCTGGCCGc                                              <  1:397580/68‑1 (MQ=255)
                  gCGATTAAACGCCTGGCGAACGTTGCCAGCAATGTGTGTGGCTGGAGGCTTGCTGATGTGCTGGCCGc                                              <  1:1481030/68‑1 (MQ=255)
                  gCGATTAAACGCCTGGCGAACGTTGCCAGCAATGTGTGTGGCTGGAGGCTTGCTGATGTGCTGGCCGc                                              <  1:165323/68‑1 (MQ=255)
                  gCGATTAAACGCCTGGCGAACGTTGCCAGCAATGTGTGTGGCTGGAGGCTTGCTGATGTGCTGGCCGc                                              <  1:479510/68‑1 (MQ=255)
                  gCGATTAAACGCCTGGCGAACGTTGCCAGCAATGTGTGTGGCTGGAGGCTTGCTGATGTGCTGGCCGc                                              <  1:589511/68‑1 (MQ=255)
                  gCGATTAAACGCCTGGCGAACGTTGCCAGCAATGTGTGTGGCTGGAGGCTTGCTGATGTGCTGGCCGc                                              <  1:1971478/68‑1 (MQ=255)
                  gCGATTAAACGCCTGGCGAACGTTGCCAGCAATGTGTGTGGCTGGAGGCTTGCTGATGTGCTGGCCGc                                              <  1:1237151/68‑1 (MQ=255)
                  gCGATTAAACGCCTGGCGAACGTTGCCAGCAATGTGTGTGGCTGGAGGCTTGCTGATGTGCTGGCCGc                                              <  1:1148846/68‑1 (MQ=255)
                  gCGATTAAACGCCTGGCGAACGTTGCCAGCAATGTGTGTGGCTGGAGGCTTGCTGATGTGCTGGCCGc                                              <  1:84205/68‑1 (MQ=255)
                  gCGATTAAACGCCTGGCGAACGTTGCCAGCAATGTGTGTGGCTGGAGGCTTGCTGATGTGCTGGCCGc                                              <  1:860983/68‑1 (MQ=255)
                  gCGATTAAACGCCTGGCGAACGTTGCCAGCAATGTGTGTGGCTGGAGGCTTGCTGATGTGCTGGCCGc                                              <  1:1132142/68‑1 (MQ=255)
                  gCGATTAAACGCCTGGCGAACGTTGCCAGCAATGTGTGTGGCTGGAGGCTTGCTGATGTGCTGGCCGc                                              <  1:1111310/68‑1 (MQ=255)
                  gCGATTAAACGCCTGGCGAACGTTGCCAGCAATGTGTGTGGCTGGAGGCTTGCTGATGTGCTGGCCGCt                                             >  1:702286/1‑69 (MQ=255)
                  gCGATTAAACGCCTGGCGAACGTTGCCAGCAATGTGTGTGGCTGGAGGCTTGCTGATGTGCTGGCCGCt                                             >  1:2574187/1‑69 (MQ=255)
                  gCGATTAAACGCCTGGCGAACGTTGCCAGCAATGTGTGTGGCTGGAGGCTTGCTGATGTGCTGGCCGCt                                             >  1:840577/1‑69 (MQ=255)
                  gCGATTAAACGCCTGGCGAACGTTGCCAGCAATGTGTGTGGCTGGAGGCTTGCTGATGTGCTGGCCGCt                                             >  1:3032558/1‑69 (MQ=255)
                  gCGATTAAACGCCTGGCGAACGTTGCCAGCAATGTGTGTGGCTGGAGGCTTGCTGATGTGCTGGCCGCt                                             >  1:466906/1‑69 (MQ=255)
                  gCGATTAAACGCCTGGCGAACGTTGCCAGCAATGTGTGTGGCTGGAGGCTTGCTGATGTGCTGGCCGCt                                             >  1:2269619/1‑69 (MQ=255)
                       tAAACGCCTGGCGAACGTTGCCAGCAATGTGTGTGGCTGGAGGCTTGCTGATGTGCTGGCCGCTGTgg                                         >  1:1564562/1‑68 (MQ=255)
                       tAAACGCCTGGCGAACGTTGCCAGCAATGTGTGTGGCTGGAGGCTTGCTGATGTGCTGGCCGCTGTgg                                         >  1:152484/1‑68 (MQ=255)
                       tAAACGCCTGGCGAACGTTGCCAGCAATGTGTGTGGCTGGAGGCTTGCTGATGTGCTGGCCGCTGTgg                                         >  1:3394513/1‑68 (MQ=255)
                       tAAACGCCTGGCGAACGTTGCCAGCAATGTGTGTGGCTGGAGGCTTGCTGATGTGCTGGCCGCTGTgg                                         >  1:1828281/1‑68 (MQ=255)
                       tAAACGCCTGGCGAACGTTGCCAGCAATGTGTGTGGCTGGAGGCTTGCTGATGTGCTGGCCGCTGTgg                                         >  1:462513/1‑68 (MQ=255)
                              cTGGCGAACGTTGCCAGCAATGTGTGTGGCTGTAGGCTTGCTGAt                                                         <  1:1479571/45‑1 (MQ=255)
                              cTGGCGAACGTTGCCAGCAATGTGTGTGGCTGTAGGCTTGCTGAt                                                         <  1:3418411/45‑1 (MQ=255)
                              cTGGCGAACGTTGCCAGCAATGTGTGTGGCTGTAGGCTTGCTGAt                                                         <  1:1356762/45‑1 (MQ=255)
                              cTGGCGAACGTTGCCAGCAATGTGTGTGGCTGTAGGCTTGCTGAt                                                         <  1:1308800/45‑1 (MQ=255)
                              cTGGCGAACGTTGCCAGCAATGTGTGTGGCTGTAGGCTTGCTGAt                                                         <  1:3325786/45‑1 (MQ=255)
                              cTGGCGAACGTTGCCAGCAATGTGTGTGGCTGTAGGCTTGCTGAt                                                         <  1:2339231/45‑1 (MQ=255)
                              cTGGCGAACGTTGCCAGCAATGTGTGTGGCTGTAGGCTTGCTGAt                                                         <  1:2487979/45‑1 (MQ=255)
                              cTGGCGAACGTTGCCAGCAATGTGTGTGGCTGTAGGCTTGCTGAt                                                         <  1:2566638/45‑1 (MQ=255)
                              cTGGCGAACGTTGCCAGCAATGTGTGTGGCTGTAGGCTTGCTGAt                                                         <  1:3267477/45‑1 (MQ=255)
                              cTGGCGAACGTTGCCAGCAATGTGTGTGGCTGTAGGCTTGCTGAt                                                         <  1:3266332/45‑1 (MQ=255)
                              cTGGCGAACGTTGCCAGCAATGTGTGTGGCTGTAGGCTTGCTGAt                                                         <  1:3232224/45‑1 (MQ=255)
                              cTGGCGAACGTTGCCAGCAATGTGTGTGGCTGTAGGCTTGCTGAt                                                         <  1:2145777/45‑1 (MQ=255)
                              cTGGCGAACGTTGCCAGCAATGTGTGTGGCTGTAGGCTTGCTGAt                                                         <  1:3133042/45‑1 (MQ=255)
                              cTGGCGAACGTTGCCAGCAATGTGTGTGGCTGTAGGCTTGCTGAt                                                         <  1:2141061/45‑1 (MQ=255)
                              cTGGCGAACGTTGCCAGCAATGTGTGTGGCTGTAGGCTTGCTGAt                                                         <  1:2858168/45‑1 (MQ=255)
                              cTGGCGAACGTTGCCAGCAATGTGTGTGGCTGTAGGCTTGCTGAt                                                         <  1:3375827/45‑1 (MQ=255)
                                  cGAACTTTGCCAGCAATGTGTGTGGCTGTAGGCTTGCTGAt                                                         <  1:2910595/41‑1 (MQ=255)
                                             aGCAATGTGTGTGGCTGGAGGCTTGCTGATGTGCTGGCCGCTGTGGCAAAAACCTCGACCTGACGAGt                   <  1:1769648/68‑1 (MQ=255)
                                                    tgtgtgGCTGGAGGCTTGCTGATGTGCTGGCCGCTGTGGCAAAAACCTCGACCTGACGAGTGGCAGCTg           >  1:190689/1‑69 (MQ=255)
                                                    tgtgtgGCTGGAGGCTTGCTGATGTGCTGGCCGCTGTGGCAAAAACCTCGACCTGACGAGTGGCAGCTg           >  1:1608693/1‑69 (MQ=255)
                                                      tgtgGCTGGAGGCTTGCTGATGTGCTGGCCGCTGTGGCAAAAACCTCGACCTGACGAGTGGCAGCTGTa         <  1:3051212/69‑1 (MQ=255)
                                                          gCTGGAGGCTTGCTGATGTGCTGGCCGCTGTGGCAAAAACCTCGACCTGACGAGTGGCAGCTGTACATg     >  1:2635057/1‑69 (MQ=255)
                                                             ggAGGCTTGCTGATGTGCTGGCCGCTGTGGCAAAAACCTCGACCTGACGAGTGGCAGCTGTACATGCtt  <  1:1944651/69‑1 (MQ=255)
                                                             ggAGGCTTGCTGATGTGCTGGCCGCTGTGGCAAAAACCTCGACCTGACGAGTGGCAGCTGTACATGCtt  <  1:764880/69‑1 (MQ=255)
                                                             ggAGGCTTGCTGATGTGCTGGCCGCTGTGGCAAAAACCTCGACCTGACGAGTGGCAGCTGTACATGCtt  <  1:2267402/69‑1 (MQ=255)
                                                             ggAGGCTTGCTGATGTGCTGGCCGCTGTGGCAAAAACCTCGACCTGACGAGTGGCAGCTGTACATGCtt  <  1:2539984/69‑1 (MQ=255)
                                                             ggAGGCTTGCTGATGTGCTGGCCGCTGTGGCAAAAACCTCGACCTGACGAGTGGCAGCTGTACATGCtt  <  1:2378567/69‑1 (MQ=255)
                                                              |                                                                   
GTTCTTACTGGTGGTATGGCGATTAAACGCCTGGCGAACGTTGCCAGCAATGTGTGTGGCTGGAGGCTTGCTGATGTGCTGGCCGCTGTGGCAAAAACCTCGACCTGACGAGTGGCAGCTGTACATGCTT  >  minE/622401‑622530

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: