| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 626,245 | C→T | 30.8% | A53V (GCC→GTC) | ycaQ → | conserved hypothetical protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 626,245 | 0 | C | T | 30.8% | 78.8 / 39.0 | 84 | A53V (GCC→GTC) | ycaQ | conserved hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base C (41/17); new base T (21/5); total (62/22) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.26e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.89e-01 | |||||||||||
GGAGGATATTCCGGCAACGATCTCCCGCATGTCCTTGCTGCAAATCGATACCATCAATATTGTTGCCCGTAGTCCATATCTGGTGCTTTTCAGTCGTCTGGGAAATTATCCTGCCCAGTGGCTGGATGAGTC > minE/626180‑626311 | ggaggaTATTCCGGCAACGATCTCCCGCATGTCCTTGCTGCAAATCGATACCATCAATATTGTTGCCCg < 1:1995688/69‑1 (MQ=255)ggaggaTATTCCGGCAACGATCTCCCGCATGTCCTTGCTGCAAATCGATACCATCAATATTGTTGCCCg < 1:1933727/69‑1 (MQ=255)ggaggaTATTCCGGCAACGATCTCCCGCATGTCCTTGCTGCAAATCGATACCATCAATATTGTTGCCCg < 1:1918387/69‑1 (MQ=255)ggaggaTATTCCGGCAACGATCTCCCGCATGTCCTTGCTGCAAATCGATACCATCAATATTGTTGCCCg < 1:1219705/69‑1 (MQ=255)ggaggaTATTCCGGCAACGATCTCCCGCATGTCCTTGCTGCAAATCGATACCATCAATATTGTTGCCCg < 1:2193655/69‑1 (MQ=255)ggaggaTATTCCGGCAACGATCTCCCGCATGTCCTTGCTGCAAATCGATACCATCAATATTGTTGCCCg < 1:766844/69‑1 (MQ=255)ggaggaTATTCCGGCAACGATCTCCCGCATGTCCTTGCTGCAAATCGATACCATCAATATTGTTGCCCg < 1:1373009/69‑1 (MQ=255)ggaggaTATTCCGGCAACGATCTCCCGCATGTCCTTGCTGCAAATCGATACCATCAATATTGTTGCCCg < 1:2490590/69‑1 (MQ=255)ggaggaTATTCCGGCAACGATCTCCCGCATGTCCTTGCTGCAAATCGATACCATCAATATTGTTGCCCg < 1:1480974/69‑1 (MQ=255)ggaggaTATTCCGGCAACGATCTCCCGCATGTCCTTGCTGCAAATCGATACCATCAATATTGTTGCCCg < 1:549867/69‑1 (MQ=255) aaCGATCTCCCGCATGTCCTTGCTGCAAATCGATACCATCAATATTGTTGCCCGTAGTCCata > 1:3061850/1‑63 (MQ=255) aaCGATCTCCCGCATGTCCTTGCTGCAAATCGATACCATCAATATTGTTGCCCGTAGTCCata > 1:2045079/1‑63 (MQ=255) aaCGATCTCCCGCATGTCCTTGCTGCAAATCGATACCATCAATATTGTTGCCCGTAGTCCata > 1:3321397/1‑63 (MQ=255) aaCGATCTCCCGCATGTCCTTGCTGCAAATCGATACCATCAATATTGTTGCCCGTAGTCCata > 1:1669969/1‑63 (MQ=255) aaCGATCTCCCGCATGTCCTTGCTGCAAATCGATACCATCAATATTGTTGCCCGTAGTCCata > 1:2369220/1‑63 (MQ=255) aaCGATCTCCCGCATGTCCTTGCTGCAAATCGATACCATCAATATTGTTGCCCGTAGTCCata > 1:1580546/1‑63 (MQ=255) aaCGATCTCCCGCATGTCCTTGCTGCAAATCGATACCATCAATATTGTTGCCCGTAGTCCata > 1:814954/1‑63 (MQ=255) aaCGATCTCCCGCATGTCCTTGCTGCAAATCGATACCATCAATATTGTTGCCCGTAGTCCata > 1:1136334/1‑63 (MQ=255) aaCGATCTCCCGCATGTCCTTGCTGCAAATCGATACCATCAATATTGTTGCCCGTAGTCCata > 1:1110899/1‑63 (MQ=255) ctcCCGCATGTCCTTGCTGCAAATCGATACCATCAATATTGTTGCCCGTAGTCCATATCTGGTGCtt > 1:436497/1‑67 (MQ=255) ctcCCGCATGTCCTTGCTGCAAATCGATACCATCAATATTGTTGCCCGTAGTCCATATCTGGTGCtt > 1:2471243/1‑67 (MQ=255) ctcCCGCATGTCCTTGCTGCAAATCGATACCATCAATATTGTTGCCCGTAGTCCATATCTGGTGCtt > 1:3182070/1‑67 (MQ=255) ctcCCGCATGTCCTTGCTGCAAATCGATACCATCAATATTGTTGCCCGTAGTCCATATCTGGTGCtt > 1:1004560/1‑67 (MQ=255) ctcCCGCATGTCCTTGCTGCAAATCGATACCATCAATATTGTTGCCCGTAGTCCATATCTGGTGCtt > 1:3319018/1‑67 (MQ=255) ctcCCGCATGTCCTTGCTGCAAATCGATACCATCAATATTGTTGCCCGTAGTCCATATCTGGTGCtt > 1:298074/1‑67 (MQ=255) ctcCCGCATGTCCTTGCTGCAAATCGATACCATCAATATTGTTGCCCGTAGTCCATATCTGGTGCtt > 1:2915016/1‑67 (MQ=255) ctcCCGCATGTCCTTGCTGCAAATCGATACCATCAATATTGTTGCCCGTAGTCCATATCTGGTGCtt > 1:2808785/1‑67 (MQ=255) ctcCCGCATGTCCTTGCTGCAAATCGATACCATCAATATTGTTGCCCGTAGTCCATATCTGGTGCtt > 1:1452750/1‑67 (MQ=255) ctcCCGCATGTCCTTGCTGCAAATCGATACCATCAATATTGTTGCCCGTAGTCCATATCTGGTGCtt > 1:984983/1‑67 (MQ=255) ctcCCGCATGTCCTTGCTGCAAATCGATACCATCAATATTGTTGCCCGTAGTCCATATCTGGTGCtt > 1:889318/1‑67 (MQ=255) ctcCCGCATGTCCTTGCTGCAAATCGATACCATCAATATTGTTGCCCGTAGTCCATATCTGGTGCtt > 1:1624778/1‑67 (MQ=255) tGTCCTTGCTGCAAATCGATACCATCAATATTGTTGCCCGTAGTCCATATCTGGTGCTTTTCAgtcgtc > 1:3257880/1‑69 (MQ=255) tGTCCTTGCTGCAAATCGATACCATCAATATTGTTGCCCGTAGTCCATATCTGGTGCTTTTCAgtcgtc > 1:133103/1‑69 (MQ=255) tGTCCTTGCTGCAAATCGATACCATCAATATTGTTGCCCGTAGTCCATATCTGGTGCTTTTCAgtcgt > 1:564396/1‑68 (MQ=255) tCCTTGCTGCAAATCGATACCATCAATATTGTTGCCCGTAGTCCATATCTGGTGCTTTTCAGTCGTCt > 1:3436353/1‑68 (MQ=255) tCCTTGCTGCAAATCGATACCATCAATATTGTTGCCCGTAGTCCATATCTGGTGCTTTTCAGTCGTCt > 1:966354/1‑68 (MQ=255) tCCTTGCTGCAAATCGATACCATCAATATTGTTGCCCGTAGTCCATATCTGGTGCTTTTCAGTCGTCt > 1:2862215/1‑68 (MQ=255) aaaTCGATACCATCAATATTGTTGTCCGTAGCCCATATCTGGTGCTTTTCAgccg > 1:1685817/1‑55 (MQ=255) aaaTCGATACCATCAATATTGTTGTCCGTAGCCCATATCTGGTGCTTTTCAGCCGTCTGGGAGATTAt > 1:1746876/1‑68 (MQ=255) aaaTCGATACCATCAATATTGTTGTCCGTAGCCCATATCTGGTGCTTTTCAGCCGTCTGGGAGATTATc > 1:484176/1‑69 (MQ=255) aaaTCGATACCATCAATATTGTTGTCCGTAGCCCATATCTGGTGCTTTTCAGCCGTCTGGGAGATTATc > 1:516147/1‑69 (MQ=255) aaaTCGATACCATCAATATTGTTGTCCGTAGCCCATATCTGGTGCTTTTCAGCCGTCTGGGAGATTATc > 1:3362860/1‑69 (MQ=255) aaaTCGATACCATCAATATTGTTGTCCGTAGCCCATATCTGGTGCTTTTCAGCCGTCTGGGAGATTATc > 1:594451/1‑69 (MQ=255) aaaTCGATACCATCAATATTGTTGTCCGTAGCCCATATCTGGTGCTTTTCAGCCGTCTGGGAGATTATc > 1:3017598/1‑69 (MQ=255) aaaTCGATACCATCAATATTGTTGTCCGTAGCCCATATCTGGTGCTTTTCAGCCGTCTGGGAGATTATc > 1:2997019/1‑69 (MQ=255) aaaTCGATACCATCAATATTGTTGTCCGTAGCCCATATCTGGTGCTTTTCAGCCGTCTGGGAGATTATc > 1:3420740/1‑69 (MQ=255) aaaTCGATACCATCAATATTGTTGTCCGTAGCCCATATCTGGTGCTTTTCAGCCGTCTGGGAGATTATc > 1:2577171/1‑69 (MQ=255) aaaTCGATACCATCAATATTGTTGTCCGTAGCCCATATCTGGTGCTTTTCAGCCGTCTGGGAGATTATc > 1:2738872/1‑69 (MQ=255) aaaTCGATACCATCAATATTGTTGTCCGTAGCCCATATCTGGTGCTTTTCAGCCGTCTGGGAGATTATc > 1:1093865/1‑69 (MQ=255) aaaTCGATACCATCAATATTGTTGTCCGTAGCCCATATCTGGTGCTTTTCAGCCGTCTGGGAGATTATc > 1:131070/1‑69 (MQ=255) aaaTCGATACCATCAATATTGTTGTCCGTAGCCCATATCTGGTGCTTTTCAGCCGTCTGGGAGATTATc > 1:1393987/1‑69 (MQ=255) aaaTCGATACCATCAATATTGTTGTCCGTAGCCCATATCTGGTGCTTTTCAGCCGTCTGGGAGATTATc > 1:1523200/1‑69 (MQ=255) aaaTCGATACCATCAATATTGTTGTCCGTAGCCCATATCTGGTGCTTTTCAGCCGTCTGGGAGATTATc > 1:1731285/1‑69 (MQ=255) aaaTCGATACCATCAATATTGTTGTCCGTAGCCCATATCTGGTGCTTTTCAGCCGTCTGGGAGATTATc > 1:2307125/1‑69 (MQ=255) aaaTCGATACCATCAATATTGTTGTCCGTAGCCCATATCTGGTGCTTTTCAGCCGTCTGGGAGATTATc > 1:2237045/1‑69 (MQ=255) aaaTCGATACCATCAATATTGTTGTCCGTAGCCCATATCTGGTGCTTTTCAGCCGTCTGGGAGATTATc > 1:1819781/1‑69 (MQ=255) aaaTCGATACCATCAATATTGTTGTCCGTAGCCCATATCTGGTGCTTTTCAGCCGTCTGGGAGATTATc > 1:2234190/1‑69 (MQ=255) aaaTCGATACCATCAATATTGTTGTCCGTAGCCCATATCTGGTGCTTCTCAGCCGTCTGGGAGATTATc > 1:2102096/1‑69 (MQ=255) aaTCGATACCATCAATATTGTTGTCCGTAGCCCATATCTGGTGCTTTTCAGCCGTCTGTGAGATTATc < 1:3004942/68‑1 (MQ=255) aaTCGATACCATCAATATTGTTGTCCGTAGCCCATATCTGGTGCTTTTCAGCCGTCTGGGAGATTATc < 1:885545/68‑1 (MQ=255) aaTCGATACCATCAATATTGTTGTCCGTAGCCCATATCTGGTGCTTTTCAGCCGTCTGGGAGATTATc < 1:2498651/68‑1 (MQ=255) aaTCGATACCATCAATATTGTTGTCCGTAGCCCATATCTGGTGCTTTTCAGCCGTCTGGGAGATTATc < 1:533575/68‑1 (MQ=255) aaTCGATACCATCAATATTGTTGTCCGTAGCCCATATCTGGTGCTTTTCAGCCGTCTGGGAGATTATc < 1:5181/68‑1 (MQ=255) tCGATACCATCAATATTGTTGCCCGTAGTCCATATCTGGTGCTTTTCAGTCGTCTGGGAAATTATCCTg > 1:867053/1‑69 (MQ=255) tCGATACCATCAATATTGTTGCCCGTAGTCCATATCTGGTGCTTTTCAGTCGTCTGGGAAATTATCCTg > 1:3360344/1‑69 (MQ=255) tCGATACCATCAATATTGTTGCCCGTAGTCCATATCTGGTGCTTTTCAGTCGTCTGGGAAATTATCCTg > 1:1427952/1‑69 (MQ=255) aaTATTGTTGCCCGTAGTCCATATCTGGTGCTTTTCAGTCGTCTGGGAAATTATCCTGCCCAGTGGCTg > 1:2216587/1‑69 (MQ=255) aaTATTGTTGCCCGTAGTCCATATCTGGTGCTTTTCAGTCGTCTGGGAAATTATCCTGCCCAGTGGCTg > 1:757102/1‑69 (MQ=255) aaTATTGTTGCCCGTAGTCCATATCTGGTGCTTTTCAGTCGTCTGGGAAATTATCCTGCCCAGTGGCTg > 1:3272872/1‑69 (MQ=255) aaTATTGTTGCCCGTAGTCCATATCTGGTGCTTTTCAGTCGTCTGGGAAATTATCCTGCCCAGTGGCTg > 1:349397/1‑69 (MQ=255) ttgttgCCCGTAGTCCATATCTGGTGCTTTTCAGTCGTCTGGGAAATTATCCTGCCCAGTGGCTGGATg > 1:1653515/1‑69 (MQ=255) ttgttgCCCGTAGTCCATATCTGGTGCTTTTCAGTCGTCTGGGAAATTATCCTGCCCAGTGGCTGGATg > 1:2808472/1‑69 (MQ=255) ttgttgCCCGTAGTCCATATCTGGTGCTTTTCAGTCGTCTGGGAAATTATCCTGCCCAGTGGCTGGATg > 1:3324505/1‑69 (MQ=255) ttgttgCCCGTAGTCCATATCTGGTGCTTTTCAGTCGTCTGGGAAATTATCCTGCCCAGTGGCTGGATg > 1:3293259/1‑69 (MQ=255) ttgttgCCCGTAGTCCATATCTGGTGCTTTTCAGTCGTCTGGGAAATTATCCTGCCCAGTGGCTGGATg > 1:1306717/1‑69 (MQ=255) ttgttgCCCGTAGTCCATATCTGGTGCTTTTCAGTCGTCTGGGAAATTATCCTGCCCAGTGGCTGGATg > 1:2730976/1‑69 (MQ=255) ttgttgCCCGTAGTCCATATCTGGTGCTTTTCAGTCGTCTGGGAAATTATCCTGCCCAGTGGCTGGATg > 1:1066277/1‑69 (MQ=255) gttgCCCGTAGTCCATATCTGGTGCTTTTCAGTCGTCTGGGAAATTATCCTGCCCAGTGGCTGGATGa < 1:1999733/68‑1 (MQ=255) gttgCCCGTAGTCCATATCTGGTGCTTTTCAGTCGTCTGGGAAATTATCCTGCCCAGTGGCTGGATGa < 1:2149632/68‑1 (MQ=255) gttgCCCGTAGTCCATATCTGGTGCTTTTCAGTCGTCTGGGAAATTATCCTGCCCAGTGGCTGGATGa < 1:2574915/68‑1 (MQ=255) gCCCGTAGTCCATATCTGGTGCTTTTCAGTCGTCTGGGAAATTATCCTGCCCAGTGGCTGGATGAGtc < 1:2382793/68‑1 (MQ=255) gCCCGTAGTCCATATCTGGTGCTTTTCAGTCGTCTGGGAAATTATCCTGCCCAGTGGCTGGATGAGtc < 1:3352669/68‑1 (MQ=255) gCCCGTAGTCCATATCTGGTGCTTTTCAGTCGTCTGGGAAATTATCCTGCCCAGTGGCTGGATGAGtc < 1:3325009/68‑1 (MQ=255) gCCCGTAGTCCATATCTGGTGCTTTTCAGTCGTCTGGGAAATTATCCTGCCCAGTGGCTGGATGAGtc < 1:1357729/68‑1 (MQ=255) | GGAGGATATTCCGGCAACGATCTCCCGCATGTCCTTGCTGCAAATCGATACCATCAATATTGTTGCCCGTAGTCCATATCTGGTGCTTTTCAGTCGTCTGGGAAATTATCCTGCCCAGTGGCTGGATGAGTC > minE/626180‑626311 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |