| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 626,283 | A→G | 28.7% | N66D (AAT→GAT) | ycaQ → | conserved hypothetical protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 626,283 | 0 | A | G | 28.7% | 85.0 / 38.4 | 87 | N66D (AAT→GAT) | ycaQ | conserved hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base A (36/26); new base G (20/5); total (56/31) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 8.23e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.91e-01 | |||||||||||
AAATCGATACCATCAATATTGTTGCCCGTAGTCCATATCTGGTGCTTTTCAGTCGTCTGGGAAATTATCCTGCCCAGTGGCTGGATGAGTCTCTGGCGCGTGGCGAATTAATGGAATACTGGGCG > minE/626221‑626345 | aaaTCGATACCATCAATATTGTTGTCCGTAGCCCATATCTGGTGCTTTTCAGCCGTCTGGGAGATTAt > 1:1746876/1‑68 (MQ=255)aaaTCGATACCATCAATATTGTTGTCCGTAGCCCATATCTGGTGCTTTTCAGCCGTCTGGGAGATTATc > 1:516147/1‑69 (MQ=255)aaaTCGATACCATCAATATTGTTGTCCGTAGCCCATATCTGGTGCTTTTCAGCCGTCTGGGAGATTATc > 1:2577171/1‑69 (MQ=255)aaaTCGATACCATCAATATTGTTGTCCGTAGCCCATATCTGGTGCTTTTCAGCCGTCTGGGAGATTATc > 1:1731285/1‑69 (MQ=255)aaaTCGATACCATCAATATTGTTGTCCGTAGCCCATATCTGGTGCTTTTCAGCCGTCTGGGAGATTATc > 1:2997019/1‑69 (MQ=255)aaaTCGATACCATCAATATTGTTGTCCGTAGCCCATATCTGGTGCTTTTCAGCCGTCTGGGAGATTATc > 1:484176/1‑69 (MQ=255)aaaTCGATACCATCAATATTGTTGTCCGTAGCCCATATCTGGTGCTTTTCAGCCGTCTGGGAGATTATc > 1:1523200/1‑69 (MQ=255)aaaTCGATACCATCAATATTGTTGTCCGTAGCCCATATCTGGTGCTTTTCAGCCGTCTGGGAGATTATc > 1:594451/1‑69 (MQ=255)aaaTCGATACCATCAATATTGTTGTCCGTAGCCCATATCTGGTGCTTTTCAGCCGTCTGGGAGATTATc > 1:3017598/1‑69 (MQ=255)aaaTCGATACCATCAATATTGTTGTCCGTAGCCCATATCTGGTGCTTTTCAGCCGTCTGGGAGATTATc > 1:1393987/1‑69 (MQ=255)aaaTCGATACCATCAATATTGTTGTCCGTAGCCCATATCTGGTGCTTTTCAGCCGTCTGGGAGATTATc > 1:2307125/1‑69 (MQ=255)aaaTCGATACCATCAATATTGTTGTCCGTAGCCCATATCTGGTGCTTTTCAGCCGTCTGGGAGATTATc > 1:1819781/1‑69 (MQ=255)aaaTCGATACCATCAATATTGTTGTCCGTAGCCCATATCTGGTGCTTTTCAGCCGTCTGGGAGATTATc > 1:2237045/1‑69 (MQ=255)aaaTCGATACCATCAATATTGTTGTCCGTAGCCCATATCTGGTGCTTTTCAGCCGTCTGGGAGATTATc > 1:131070/1‑69 (MQ=255)aaaTCGATACCATCAATATTGTTGTCCGTAGCCCATATCTGGTGCTTTTCAGCCGTCTGGGAGATTATc > 1:3362860/1‑69 (MQ=255)aaaTCGATACCATCAATATTGTTGTCCGTAGCCCATATCTGGTGCTTTTCAGCCGTCTGGGAGATTATc > 1:3420740/1‑69 (MQ=255)aaaTCGATACCATCAATATTGTTGTCCGTAGCCCATATCTGGTGCTTTTCAGCCGTCTGGGAGATTATc > 1:2738872/1‑69 (MQ=255)aaaTCGATACCATCAATATTGTTGTCCGTAGCCCATATCTGGTGCTTTTCAGCCGTCTGGGAGATTATc > 1:1093865/1‑69 (MQ=255)aaaTCGATACCATCAATATTGTTGTCCGTAGCCCATATCTGGTGCTTTTCAGCCGTCTGGGAGATTATc > 1:2234190/1‑69 (MQ=255)aaaTCGATACCATCAATATTGTTGTCCGTAGCCCATATCTGGTGCTTCTCAGCCGTCTGGGAGATTATc > 1:2102096/1‑69 (MQ=255) aaTCGATACCATCAATATTGTTGTCCGTAGCCCATATCTGGTGCTTTTCAGCCGTCTGTGAGATTATc < 1:3004942/68‑1 (MQ=255) aaTCGATACCATCAATATTGTTGTCCGTAGCCCATATCTGGTGCTTTTCAGCCGTCTGGGAGATTATc < 1:5181/68‑1 (MQ=255) aaTCGATACCATCAATATTGTTGTCCGTAGCCCATATCTGGTGCTTTTCAGCCGTCTGGGAGATTATc < 1:533575/68‑1 (MQ=255) aaTCGATACCATCAATATTGTTGTCCGTAGCCCATATCTGGTGCTTTTCAGCCGTCTGGGAGATTATc < 1:2498651/68‑1 (MQ=255) aaTCGATACCATCAATATTGTTGTCCGTAGCCCATATCTGGTGCTTTTCAGCCGTCTGGGAGATTATc < 1:885545/68‑1 (MQ=255) tCGATACCATCAATATTGTTGCCCGTAGTCCATATCTGGTGCTTTTCAGTCGTCTGGGAAATTATCCTg > 1:1427952/1‑69 (MQ=255) tCGATACCATCAATATTGTTGCCCGTAGTCCATATCTGGTGCTTTTCAGTCGTCTGGGAAATTATCCTg > 1:867053/1‑69 (MQ=255) tCGATACCATCAATATTGTTGCCCGTAGTCCATATCTGGTGCTTTTCAGTCGTCTGGGAAATTATCCTg > 1:3360344/1‑69 (MQ=255) aaTATTGTTGCCCGTAGTCCATATCTGGTGCTTTTCAGTCGTCTGGGAAATTATCCTGCCCAGTGGCTg > 1:2216587/1‑69 (MQ=255) aaTATTGTTGCCCGTAGTCCATATCTGGTGCTTTTCAGTCGTCTGGGAAATTATCCTGCCCAGTGGCTg > 1:3272872/1‑69 (MQ=255) aaTATTGTTGCCCGTAGTCCATATCTGGTGCTTTTCAGTCGTCTGGGAAATTATCCTGCCCAGTGGCTg > 1:757102/1‑69 (MQ=255) aaTATTGTTGCCCGTAGTCCATATCTGGTGCTTTTCAGTCGTCTGGGAAATTATCCTGCCCAGTGGCTg > 1:349397/1‑69 (MQ=255) ttgttgCCCGTAGTCCATATCTGGTGCTTTTCAGTCGTCTGGGAAATTATCCTGCCCAGTGGCTGGATg > 1:2730976/1‑69 (MQ=255) ttgttgCCCGTAGTCCATATCTGGTGCTTTTCAGTCGTCTGGGAAATTATCCTGCCCAGTGGCTGGATg > 1:2808472/1‑69 (MQ=255) ttgttgCCCGTAGTCCATATCTGGTGCTTTTCAGTCGTCTGGGAAATTATCCTGCCCAGTGGCTGGATg > 1:3293259/1‑69 (MQ=255) ttgttgCCCGTAGTCCATATCTGGTGCTTTTCAGTCGTCTGGGAAATTATCCTGCCCAGTGGCTGGATg > 1:3324505/1‑69 (MQ=255) ttgttgCCCGTAGTCCATATCTGGTGCTTTTCAGTCGTCTGGGAAATTATCCTGCCCAGTGGCTGGATg > 1:1066277/1‑69 (MQ=255) ttgttgCCCGTAGTCCATATCTGGTGCTTTTCAGTCGTCTGGGAAATTATCCTGCCCAGTGGCTGGATg > 1:1306717/1‑69 (MQ=255) ttgttgCCCGTAGTCCATATCTGGTGCTTTTCAGTCGTCTGGGAAATTATCCTGCCCAGTGGCTGGATg > 1:1653515/1‑69 (MQ=255) gttgCCCGTAGTCCATATCTGGTGCTTTTCAGTCGTCTGGGAAATTATCCTGCCCAGTGGCTGGATGa < 1:2149632/68‑1 (MQ=255) gttgCCCGTAGTCCATATCTGGTGCTTTTCAGTCGTCTGGGAAATTATCCTGCCCAGTGGCTGGATGa < 1:2574915/68‑1 (MQ=255) gttgCCCGTAGTCCATATCTGGTGCTTTTCAGTCGTCTGGGAAATTATCCTGCCCAGTGGCTGGATGa < 1:1999733/68‑1 (MQ=255) gCCCGTAGTCCATATCTGGTGCTTTTCAGTCGTCTGGGAAATTATCCTGCCCAGTGGCTGGATGAGtc < 1:2382793/68‑1 (MQ=255) gCCCGTAGTCCATATCTGGTGCTTTTCAGTCGTCTGGGAAATTATCCTGCCCAGTGGCTGGATGAGtc < 1:1357729/68‑1 (MQ=255) gCCCGTAGTCCATATCTGGTGCTTTTCAGTCGTCTGGGAAATTATCCTGCCCAGTGGCTGGATGAGtc < 1:3325009/68‑1 (MQ=255) gCCCGTAGTCCATATCTGGTGCTTTTCAGTCGTCTGGGAAATTATCCTGCCCAGTGGCTGGATGAGtc < 1:3352669/68‑1 (MQ=255) cGTAGTCCATATCTGGTGCTTTTCAGTCGTCTGGGAAATTATCCTGCCCAGTGGCTGGATGAGTctct < 1:1269484/68‑1 (MQ=255) cATATCTGGTGCTTTTCAGTCGTCTGGGAAATTATCCTGCCCAGTGGCTGGATGAGTCTCTGGCGCGt > 1:1410443/1‑68 (MQ=255) cATATCTGGTGCTTTTCAGTCGTCTGGGAAATTATCCTGCCCAGTGGCTGGATGAGTCTCTGGCGCGTg > 1:3215624/1‑69 (MQ=255) cATATCTGGTGCTTTTCAGTCGTCTGGGAAATTATCCTGCCCAGTGGCTGGATGAGTCTCTGGCGCGTg > 1:3068812/1‑69 (MQ=255) cATATCTGGTGCTTTTCAGTCGTCTGGGAAATTATCCTGCCCAGTGGCTGGATGAGTCTCTGGCGCGTg > 1:1334499/1‑69 (MQ=255) cATATCTGGTGCTTTTCAGTCGTCTGGGAAATTATCCTGCCCAGTGGCTGGATGAGTCTCTGGCGCGTg > 1:1731875/1‑69 (MQ=255) cATATCTGGTGCTTTTCAGTCGTCTGGGAAATTATCCTGCCCAGTGGCTGGATGAGTCTCTGGCGCGTg > 1:1770403/1‑69 (MQ=255) cATATCTGGTGCTTTTCAGTCGTCTGGGAAATTATCCTGCCCAGTGGCTGGATGAGTCTCTGGCGCGTg > 1:2634035/1‑69 (MQ=255) cATATCTGGTGCTTTTCAGTCGTCTGGGAAATTATCCTGCCCAGTGGCTGGATGAGTCTCTGGCGCGTg > 1:2120053/1‑69 (MQ=255) cATATCTGGTGCTTTTCAGTCGTCTGGGAAATTATCCTGCCCAGTGGCTGGATGAGTCTCTGGCGCGTg > 1:412140/1‑69 (MQ=255) cATATCTGGTGCTTTTCAGTCGTCTGGGAAATTATCCTGCCCAGTGGCTGGATGAGTCTCTGGCGCGTg > 1:461938/1‑69 (MQ=255) cATATCTGGTGCTTTTCAGTCGTCTGGGAAATTATCCTGCCCAGTGGCTGGATGAGTCTCTGGCGAGTg > 1:1914437/1‑69 (MQ=255) tatCTGGTGCTTTTCAGTCGTCTGGGAAATTATCCTGCCCAGTGGCTGGATGAGTCTCTGGCGCGTgg > 1:1988987/1‑68 (MQ=255) tatCTGGTGCTTTTCAGTCGTCTGGGAAATTATCCTGCCCAGTGGCTGGATGAGTCTCTGGCGCGTgg > 1:3243071/1‑68 (MQ=255) tCTGGTGCTTTTCAGTCGTCTGGGAAATTATCCTGCCCAGTGGCTGGATGAGTCTCTGGCGCGTGGCGa > 1:1276587/1‑69 (MQ=255) tCTGGTGCTTTTCAGTCGTCTGGGAAATTATCCTGCCCAGTGGCTGGATGAGTCTCTGGCGCGTGGCGa > 1:1435371/1‑69 (MQ=255) tCTGGTGCTTTTCAGTCGTCTGGGAAATTATCCTGCCCAGTGGCTGGATGAGTCTCTGGCGCGTGGCGa > 1:3215951/1‑69 (MQ=255) tCTGGTGCTTTTCAGTCGTCTGGGAAATTATCCTGCCCAGTGGCTGGATGAGTCTCTGGCGCGTGGCGa > 1:1544706/1‑69 (MQ=255) tCTGGTGCTTTTCAGTCGTCTGGGAAATTATCCTGCCCAGTGGCTGGATGAGTCTCTGGCGCGTGGCGa > 1:1634396/1‑69 (MQ=255) tCTGGTGCTTTTCAGTCGTCTGGGAAATTATCCTGCCCAGTGGCTGGATGAGTCTCTGGCGCGTGGCGa > 1:269730/1‑69 (MQ=255) tCTGGTGCTTTTCAGTCGTCTGGGAAATTATCCTGCCCAGTGGCTGGATGAGTCTCTGGCGCGTGGCGa > 1:236781/1‑69 (MQ=255) gCTTTTCAGTCGTCTGGGAAATTATCCTGCCCAGTGGCTGGATGAGTCTCTGGCGCGTGGCGAAtt < 1:3135630/66‑1 (MQ=255) gCTTTTCAGTCGTCTGGGAAATTATCCTGCCCAGTGGCTGGATGAGTCTCTGGCGCGTGGCGAAtt < 1:2618148/66‑1 (MQ=255) gCTTTTCAGTCGTCTGGGAAATTATCCTGCCCAGTGGCTGGATGAGTCTCTGGCGCGTGGCGAAtt < 1:2320527/66‑1 (MQ=255) gCTTTTCAGTCGTCTGGGAAATTATCCTGCCCAGTGGCTGGATGAGTCTCTGGCGCGTGGCGAAtt < 1:2152586/66‑1 (MQ=255) tttCAGTCGTCTGGGAAATTATCCTGCCCAGTGGCTGGATGAGTCTCTGGCGCGTGGCGAATTAATgg < 1:2193294/68‑1 (MQ=255) tttCAGTCGTCTGGGAAATTATCCTGCCCAGTGGCTGGATGAGTCTCTGGCGCGTGGCGAATTAATgg < 1:3340818/68‑1 (MQ=255) tttCAGTCGTCTGGGAAATTATCCTGCCCAGTGGCTGGATGAGTCTCTGGCGCGTGGCGAATTAATgg < 1:2035335/68‑1 (MQ=255) cAGTCGTCTGGGAAATTATCCTGCCCAGTGGCTGGATGAGTCTCTGGCGCGTGGCGAATTAATGGAAt < 1:3118812/68‑1 (MQ=255) cAGTCGTCTGGGAAATTATCCTGCCCAGTGGCTGGATGAGTCTCTGGCGCGTGGCGAATTAATGGAAt < 1:402954/68‑1 (MQ=255) gtcgtcTGGGAAATTATCCTGCCCAGTGGCTGGATGAGTCTCTGGCGCGTGGCGAATTAATGGAATAc < 1:956212/68‑1 (MQ=255) gtcgtcTGGGAAATTATCCTGCCCAGTGGCTGGATGAGTCTCTGGCGCGTGGCGAATTAATGGAATAc < 1:1876373/68‑1 (MQ=255) gtcgtcTGGGAAATTATCCTGCCCAGTGGCTGGATGAGTCTCTGGCGCGTGGCGAATTAATGGAATAc < 1:1376260/68‑1 (MQ=255) gtcgtcTGGGAAATTATCCTGCCCAGTGGCTGGATGAGTCTCTGGCGCGTGGCGAATTAATGGAATAc < 1:1348143/68‑1 (MQ=255) gtcgtcTGGGAAATTATCCTGCCCAGTGGCTGGATGAGTCTCTGGCGCGTGGCGAATTAATGGAATAc < 1:1066632/68‑1 (MQ=255) cgtcTGGGAAATTATCCTGCCCAGTGGCTGGATGAGTCTCTGGCGCGTGGCGAAtt < 1:529690/56‑1 (MQ=255) gtgtGGGAAATTATCCTGCCCAGTGGCTGGATGAGTCTCTGGCGCGTGGCGAATTAATGGAATACTggg > 1:1521844/4‑69 (MQ=255) gtcTGGGAAATTATCCTGCCCAGTGGCTGGATGAGTCTCTGGCGCGTGGCGAATTAATGGAATACTggg > 1:2626610/1‑69 (MQ=255) cTGGGAAATTATCCTGCCCAGTGGCTGGATGAGTCTCTGGCGCGTGGCGAATTAATGGAATACTGGgcg < 1:300914/69‑1 (MQ=255) cTGGGAAATTATCCTGCCCAGTGGCTGGATGAGTCTCTGGCGCGTGGCGAATTAATGGAATACTGGgcg < 1:2775429/69‑1 (MQ=255) cTGGGAAATTATCCTGCCCAGTGGCTGGATGAGTCTCTGGCGCGTGGCGAATTAATGGAATACTGGgcg < 1:1193479/69‑1 (MQ=255) | AAATCGATACCATCAATATTGTTGCCCGTAGTCCATATCTGGTGCTTTTCAGTCGTCTGGGAAATTATCCTGCCCAGTGGCTGGATGAGTCTCTGGCGCGTGGCGAATTAATGGAATACTGGGCG > minE/626221‑626345 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |