| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 626,915 | G→C | 65.4% | L276L (CTG→CTC) | ycaQ → | conserved hypothetical protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 626,915 | 0 | G | C | 65.4% | 46.2 / 39.7 | 52 | L276L (CTG→CTC) | ycaQ | conserved hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base G (7/11); new base C (12/22); total (19/33) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
TGTTGAAAAATTGGGCAATCTTTGGCTGCATGATGATTTGCTGCCGCTACTCGAGCGAGCGCTGGCCGGAAAGCTCACTGCAACGCACAGCGCGGTACTTTCGCCTTTTGATCCTGTTGTCTGGGA > minE/626852‑626977 | tGTTGAAAAATTGGGCAATCTTTGGCTGCATGATGATTTGCTGCCGCTACTCGAGCGAGCGCTGGCCg < 1:748250/68‑1 (MQ=255)tGTTGAAAAATTGGGCAATCTTTGGCTGCATGATGATTTGCTGCCGCTACTCGAGCGAGCGCTGGCCg < 1:3193305/68‑1 (MQ=255)tGTTGAAAAATTGGGCAATCTTTGGCTGCATGATGATTTGCTGCCGCTACTCGAGCGAGCGCTGGCCg < 1:3082670/68‑1 (MQ=255)tGTTGAAAAATTGGGCAATCTTTGGCTGCATGATGATTTGCTGCCGCTACTCGAGCGAGCGCTGGCCg < 1:2583547/68‑1 (MQ=255) ttGAAAAATTGGGCAATCTTTGGCTGCATGATGATTTGCTGCCGCTACTCGAGCGAGCGCTGGCCGGaa > 1:2601493/1‑69 (MQ=255) ttGAAAAATTGGGCAATCTTTGGCTGCATGATGATTTGCTGCCGCTACTCGAGCGAGCGCTGGCCGGaa > 1:2646304/1‑69 (MQ=255) ttGAAAAATTGGGCAATCTTTGGCTGCATGATGATTTGCTGCCGCTACTCGAGCGAGCGCTGGCCGGaa > 1:2657764/1‑69 (MQ=255) ttGAAAAATTGGGCAATCTTTGGCTGCATGATGATTTGCTGCCGCTACTCGAGCGAGCGCTGGCCGGaa > 1:1468522/1‑69 (MQ=255) ttGAAAAATTGGGCAATCTTTGGCTGCATGATGATTTGCTGCCGCTACTCGAGCGAGCGCTGGCCGGaa > 1:2809236/1‑69 (MQ=255) ttGAAAAATTGGGCAATCTTTGGCTGCATGATGATTTGCTGCCGCTACTCGAGCGAGCGCTGGCCGGa > 1:27717/1‑68 (MQ=255) aaaTTGGGCAATCTTTGGCTGCATGATGATTTGCTGCCGCTACTCGAGCGAGCGCTGGCCGGAAAGCt < 1:2961995/68‑1 (MQ=255) aaaTTGGGCAATCTTTGGCTGCATGATGATTTGCTGCCGCTACTCGAGCGAGCGCTGGCCGGAAAGCt < 1:1470504/68‑1 (MQ=255) aaaTTGGGCAATCTTTGGCTGCATGATGATTTGCTGCCGCTACTCGAGCGAGCGCTGGCCGGAAAGCt < 1:3237825/68‑1 (MQ=255) aTCTTTGGCTGCATGATGATTTGCTGCCGCTACTCGAGCGAGCGCTGGCCGGAAAGCt < 1:1395159/58‑1 (MQ=255) cTTTGGCTGCATGATGATTTGCTGCCGCTACTCGAGCGAGCGCTGGCCGGAAAGCTCACTGCAACGcac > 1:2801924/1‑69 (MQ=255) gatTTGCTGCCGCTACTCGAGCGAGCGCTGGCCGGAAATCTCACTGCAACGCACAGCGCGGTACTTTCg < 1:2290381/69‑1 (MQ=255) gatTTGCTGCCGCTACTCGAGCGAGCGCTGGCCGGAAAGCTCACTGCAACGCACAGCGCGGTACTTTCg < 1:1886261/69‑1 (MQ=255) gatTTGCTGCCGCTACTCGAGCGAGCGCTGGCCGGAAAGCTCACTGCAACGCACAGCGCGGTACTTTCg < 1:1590780/69‑1 (MQ=255) atTTGCTGCCGCTACTCGAGCGTGCGCTCGCCGGAAAGCTCACTGCAACGCACAGCGCGGTACTTTCGc > 1:2695084/1‑69 (MQ=255) atTTGCTGCCGCTACTCGAGCGTGCGCTCGCCGGAAAGCTCACTGCAACGCACAGCGCGGTACTTTCGc > 1:699224/1‑69 (MQ=255) atTTGCTGCCGCTACTCGAGCGTGCGCTCGCCGGAAAGCTCACTGCAACGCACAGCGCGGTACTTTCGc > 1:1157551/1‑69 (MQ=255) atTTGCTGCCGCTACTCGAGCGTGCGCTCGCCGGAAAGCTCACTGCAACGCACAGCGCGGTACTTTCGc > 1:2830829/1‑69 (MQ=255) atTTGCTGCCGCTACTCGAGCGTGCGCTCGCCGGAAAGCTCACTGCAACGCACAGCGCGGTACTTTCGc > 1:2168998/1‑69 (MQ=255) atTTGCTGCCGCTACTCGAGCGTGCGCTCGCCGGAAAGCTCACTGCAACGCACAGCGCGGTACTTTCGc > 1:215915/1‑69 (MQ=255) atTTGCTGCCGCTACTCGAGCGTGCGCTCGCCGGAAAGCTCACTGCAACGCACAGCGCGGTACTTTCGc > 1:2151096/1‑69 (MQ=255) atTTGCTGCCGCTACTCGAGCGTGCGCTCGCCGGAAAGCTCACTGCAACGCACAGCGCGGTACTTTCGc > 1:75898/1‑69 (MQ=255) atTTGCTGCCGCTACTCGAGCGTGCGCTCGCCGGAAAGCTCACTGCAACGCACAGCGCGGTACTTTCGc > 1:766073/1‑69 (MQ=255) atTTGCTGCCGCTACTCGAGCGTGCGCTCGCCGGAAAGCTCACTGCAACGCACAGCGCGGTACTTTCGc > 1:828319/1‑69 (MQ=255) atTTGCTGCCGCTACTCGAGCGTGCGCTCGCCGGAAAGCTCACTGCAACGCACAGCGCGGTACTTTCGc > 1:1511110/1‑69 (MQ=255) atTTGCTGCCGCTACTCGAGCGTGCGCTCGCCGGAAAGCTCACTGCAACGCACAGCGCGGTACTTTCGc > 1:916080/1‑69 (MQ=255) tgcgcTCGCCGGAAAGCTCACTGCAACGCACAGCGCGGTACTTTCGCCTTTTGATCCTGTTGTCTGGGa < 1:3321339/68‑1 (MQ=255) tgcgcTCGCCGGAAAGCTCACTGCAACGCACAGCGCGGTACTTTCGCCTTTTGATCCTGTTGTCTGGGa < 1:277502/68‑1 (MQ=255) tgcgcTCGCCGGAAAGCTCACTGCAACGCACAGCGCGGTACTTTCGCCTTTTGATCCTGTTGTCTGGGa < 1:3348155/68‑1 (MQ=255) tgcgcTCGCCGGAAAGCTCACTGCAACGCACAGCGCGGTACTTTCGCCTTTTGATCCTGTTGTCTGGGa < 1:336794/68‑1 (MQ=255) tgcgcTCGCCGGAAAGCTCACTGCAACGCACAGCGCGGTACTTTCGCCTTTTGATCCTGTTGTCTGGGa < 1:452399/68‑1 (MQ=255) tgcgcTCGCCGGAAAGCTCACTGCAACGCACAGCGCGGTACTTTCGCCTTTTGATCCTGTTGTCTGGGa < 1:533138/68‑1 (MQ=255) tgcgcTCGCCGGAAAGCTCACTGCAACGCACAGCGCGGTACTTTCGCCTTTTGATCCTGTTGTCTGGGa < 1:593186/68‑1 (MQ=255) tgcgcTCGCCGGAAAGCTCACTGCAACGCACAGCGCGGTACTTTCGCCTTTTGATCCTGTTGTCTGGGa < 1:878160/68‑1 (MQ=255) tgcgcTCGCCGGAAAGCTCACTGCAACGCACAGCGCGGTACTTTCGCCTTTTGATCCTGTTGTCTGGGa < 1:3298202/68‑1 (MQ=255) tgcgcTCGCCGGAAAGCTCACTGCAACGCACAGCGCGGTACTTTCGCCTTTTGATCCTGTTGTCTGGGa < 1:2918049/68‑1 (MQ=255) tgcgcTCGCCGGAAAGCTCACTGCAACGCACAGCGCGGTACTTTCGCCTTTTGATCCTGTTGTCTGGGa < 1:2789082/68‑1 (MQ=255) tgcgcTCGCCGGAAAGCTCACTGCAACGCACAGCGCGGTACTTTCGCCTTTTGATCCTGTTGTCTGGGa < 1:2785209/68‑1 (MQ=255) tgcgcTCGCCGGAAAGCTCACTGCAACGCACAGCGCGGTACTTTCGCCTTTTGATCCTGTTGTCTGGGa < 1:2407972/68‑1 (MQ=255) tgcgcTCGCCGGAAAGCTCACTGCAACGCACAGCGCGGTACTTTCGCCTTTTGATCCTGTTGTCTGGGa < 1:2278347/68‑1 (MQ=255) tgcgcTCGCCGGAAAGCTCACTGCAACGCACAGCGCGGTACTTTCGCCTTTTGATCCTGTTGTCTGGGa < 1:2036245/68‑1 (MQ=255) tgcgcTCGCCGGAAAGCTCACTGCAACGCACAGCGCGGTACTTTCGCCTTTTGATCCTGTTGTCTGGGa < 1:1818519/68‑1 (MQ=255) tgcgcTCGCCGGAAAGCTCACTGCAACGCACAGCGCGGTACTTTCGCCTTTTGATCCTGTTGTCTGGGa < 1:1801980/68‑1 (MQ=255) tgcgcTCGCCGGAAAGCTCACTGCAACGCACAGCGCGGTACTTTCGCCTTTTGATCCTGTTGTCTGGGa < 1:1776138/68‑1 (MQ=255) tgcgcTCGCCGGAAAGCTCACTGCAACGCACAGCGCGGTACTTTCGCCTTTTGATCCTGTTGTCTGGGa < 1:1375448/68‑1 (MQ=255) tgcgcTCGCCGGAAAGCTCACTGCAACGCACAGCGCGGTACTTTCGCCTTTTGATCCTGTTGTCTGGGa < 1:1176303/68‑1 (MQ=255) gcgcTCGCCGGAAAGCTCACTGCAACGCACAGCGCGGTACTTTCGCCTTTTGATCCTGTTGTCTGGGa < 1:518048/68‑1 (MQ=255) gcgcTCGCCGGAAAGCTCACTGCAACGCACAGCGCGGTACTTTCGCCTTTTGATCCTGTTGTCTGGGa < 1:219263/68‑1 (MQ=255) | TGTTGAAAAATTGGGCAATCTTTGGCTGCATGATGATTTGCTGCCGCTACTCGAGCGAGCGCTGGCCGGAAAGCTCACTGCAACGCACAGCGCGGTACTTTCGCCTTTTGATCCTGTTGTCTGGGA > minE/626852‑626977 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |