| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 627,664 | T→C | 26.8% | R38R (CGT→CGC) | kdsB → | 3‑deoxy‑manno‑octulosonate cytidylyltransferase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 627,664 | 0 | T | C | 26.8% | 55.0 / 18.2 | 52 | R38R (CGT→CGC) | kdsB | 3‑deoxy‑manno‑octulosonate cytidylyltransferase |
| Reads supporting (aligned to +/- strand): ref base T (9/29); new base C (5/9); total (14/38) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.85e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.44e-01 | |||||||||||
TGCCCGGTAAACCATTGGTTGATATTAACGGCAAACCCATGATTGTTCATGTTCTTGAACGCGCGCGTGAATCAGGTGCCGAGCGCATCATCGTGGCAACCGATCATGAGGATGTTGCCCGCGCCGTTG > minE/627597‑627725 | tGCCCGGTAAACCATTGGTTGATATTAACGGCAAACCCATGATTGTTCATGTTCTTGAACGCGCGCGTg < 1:747818/69‑1 (MQ=255)tGCCCGGTAAACCATTGGTTGATATTAACGGCAAACCCATGATTGTTCATGTTCTTGAACGCGCGCGTg < 1:356347/69‑1 (MQ=255)tGCCCGGTAAACCATTGGTTGATATTAACGGCAAACCCATGATTGTTCATGTTCTTGAACGCGCGCGTg < 1:3210488/69‑1 (MQ=255)tGCCCGGTAAACCATTGGTTGATATTAACGGCAAACCCATGATTGTTCATGTTCTTGAACGCGCGCGTg < 1:2982991/69‑1 (MQ=255)tGCCCGGTAAACCATTGGTTGATATTAACGGCAAACCCATGATTGTTCATGTTCTTGAACGCGCGCGTg < 1:3106776/69‑1 (MQ=255)tGCCCGGTAAACCATTGGTTGATATTAACGGCAAACCCATGATTGTTCATGTTCTTGAACGCGCGCGTg < 1:2035811/69‑1 (MQ=255) gTAAACCATTGGTTGATATTAACGGCAAACCCATGATTGTTCATGTTCTTGAACGCGCGCGTGAATCAg > 1:456905/1‑69 (MQ=255) ttGGTTGATATTAACGGCAAACCCATGATTGTTCATGTTCTTGAACGCGCGCGTGAATCAGGTGCCGAg > 1:2818026/1‑69 (MQ=255) ttGGTTGATATTAACGGCAAACCCATGATTGTTCATGTTCTTGAACGCGCGCGTGAATCAGGTGCCGAg > 1:3171877/1‑69 (MQ=255) ttGGTTGATATTAACGGCAAACCCATGATTGTTCATGTTCTTGAACGCGCGCGTGAATCAGGTGCCGAg > 1:1461174/1‑69 (MQ=255) ttGGTTGATATTAACGGCAAACCCATGATTGTTCATGTTCTTGAACGCGCGCGTGAATCAGGTGCCGAg > 1:3046530/1‑69 (MQ=255) atTAACGGCAAACCCATGATTGTTCATGTTCTTGAACGCGCGCGTGAATCAGGTGCCGAGCGcatcat < 1:997457/68‑1 (MQ=255) atTAACGGCAAACCCATGATTGTTCATGTTCTTGAACGCGCGCGTGAATCAGGTGCCGAGCGcatcat < 1:2533928/68‑1 (MQ=255) atTAACGGCAAACCCATGATTGTTCATGTTCTTGAACGCGCGCGTGAATCAGGTGCCGAGCGcatcat < 1:2339208/68‑1 (MQ=255) atTAACGGCAAACCCATGATTGTTCATGTTCTTGAACGCGCGCGTGAATCAGGTGCCGAGCGcatcat < 1:2269023/68‑1 (MQ=255) atTAACGGCAAACCCATGATTGTTCATGTTCTTGAACGCGCGCGTGAATCAGGTGCCGAGCGcatcat < 1:1401908/68‑1 (MQ=255) atTAACGGCAAACCCATGATTGTTCATGTTCTTGAACGCGCGCGTGAATCAGGTGCCGAGCGcatcat < 1:1330004/68‑1 (MQ=255) atTAACGGCAAACCCATGATTGTTCATGTTCTTGAACGCGCGCGTGAATCAGGTGCCGAGCGcatcat < 1:594028/68‑1 (MQ=255) atTAACGGCAAACCCATGATTGTTCATGTTCTTGAACGCGCGCGTGAATCAGGTGCCGAGCGcatcat < 1:920462/68‑1 (MQ=255) aaCGGCAAACCCATGATTGTTCATGTTCTTGAACGCGCGCGTGAATCAGGTGCCGAGCGCATCATCGt > 1:2357370/1‑68 (MQ=255) aaCGGCAAACCCATGATTGTTCATGTTCTTGAACGCGCGCGTGAATCAGGTGCCGAGCGCATCATCGt > 1:2357502/1‑68 (MQ=255) aaCGGCAAACCCATGATTGTTCATGTTCTTGAACGCGCGCGTGAATCAGGTGCCGAGCGCATCATCGt > 1:2515227/1‑68 (MQ=255) aaCGGCAAACCCATGATTGTTCATGTTCTTGAACGCGCGAGTGAATCAGGTGCCGAGCGCATCATCGt > 1:2696114/1‑68 (MQ=255) ccATGATTGTTCATGTTCTTGAACGCGCGCGTGAATCAGGTGCCGAGCGCATCATCGTGGCAACCGATc < 1:2687320/69‑1 (MQ=255) ccATGATTGTTCATGTTCTTGAACGCGCGCGTGAATCAGGTGCCGAGCGCATCATCGTGGCAACCGATc < 1:2804295/69‑1 (MQ=255) ccATGATTGTTCATGTTCTTGAACGCGCGCGTGAATCAGGTGCCGAGCGCATCATCGTGGCAACCGATc < 1:2972690/69‑1 (MQ=255) ccATGATTGTTCATGTTCTTGAACGCGCGCGTGAATCAGGTGCCGAGCGCATCATCGTGGCAACCGATc < 1:372989/69‑1 (MQ=255) ccATGATTGTTCATGTTCTTGAACGCGCGCGTGAATCAGGGGCCGAGCGCATCATCGTGGCAACCGATc < 1:3376166/69‑1 (MQ=255) aTTGTTCATGTTCTTGAACGCGCGCGTGAATCAGGTGCCGAGCGCATCATCGTGGCAACCGATCATGAg < 1:2506321/69‑1 (MQ=255) aTTGTTCATGTTCTTGAACGCGCGCGTGAATCAGGTGCCGAGCGCATCATCGTGGCAACCGATCATGAg < 1:1059540/69‑1 (MQ=255) aTTGTTCATGTTCTTGAACGCGCGCGTGAATCAGGTGCCGAGCGCATCATCGTGGCAACCGATCATGAg < 1:695428/69‑1 (MQ=255) aTTGTTCATGTTCTTGAACGCGCGCGTGAATCAGGTGCCGAGCGCATCATCGTGGCAACCGATCATGAg < 1:603983/69‑1 (MQ=255) aTTGTTCATGTTCTTGAACGCGCGCGTGAATCAGGTGCCGAGCGCATCATCGTGGCAACCGATCATGAg < 1:1126311/69‑1 (MQ=255) aTTGTTCATGTTCTTGAACGCGCGCGCGAATCAGGTGCCGAGCGCATCATCGTGGc < 1:10660/56‑1 (MQ=255) aTTGTTCATGTTCTTGAACGCGCGCGCGAATCAGGTGCCGAGCGCATCATCGTGGc < 1:1070361/56‑1 (MQ=255) aTTGTTCATGTTCTTGAACGCGCGCGCGAATCAGGTGCCGAGCGCATCATCGTGGc < 1:681084/56‑1 (MQ=255) aTTGTTCATGTTCTTGAACGCGCGCGCGAATCAGGTGCCGAGCGCATCATCGTGGc < 1:665520/56‑1 (MQ=255) aTTGTTCATGTTCTTGAACGCGCGCGCGAATCAGGTGCCGAGCGCATCATCGTGGc < 1:1427589/56‑1 (MQ=255) aTTGTTCATGTTCTTGAACGCGCGCGCGAATCAGGTGCCGAGCGCATCATCGTGGc < 1:1766570/56‑1 (MQ=255) aTTGTTCATGTTCTTGAACGCGCGCGCGAATCAGGTGCCGAGCGCATCATCGTGGc < 1:2202435/56‑1 (MQ=255) aTTGTTCATGTTCTCGAACGCGCGCGCGAATCAGGTGCCGAGCGCATCATCGTGGc < 1:2849809/56‑1 (MQ=255) ttGTTCATGTTCTTGAACGCGCGCGCGAATCAGGTGCCGAGCGCATCATCGTTGc < 1:3226214/55‑1 (MQ=255) cTTGAACGCGCGCGCGAATCAGGTGCCGAGCGCATCATCGTGGCAACCGATCATGAGGATGTTGCCcgc > 1:1329512/1‑69 (MQ=255) cTTGAACGCGCGCGCGAATCAGGTGCCGAGCGCATCATCGTGGCAACCGATCATGAGGATGTTGCCcgc > 1:1176591/1‑69 (MQ=255) cTTGAACGCGCGCGCGAATCAGGTGCCGAGCGCATCATCGTGGCAACCGATCATGAGGATGTTGCCcgc > 1:2933023/1‑69 (MQ=255) cTTGAACGCGCGCGCGAATCAGGTGCCGAGCGCATCATCGTGGCAACCGATCATGAGGATGTTGCCcgc > 1:729013/1‑69 (MQ=255) cTTGAACGCGCGCGCGAATCAGGTGCCGAGCGCATCATCGTGGCAACCGATCATGAGGATGTTGCCcg > 1:1758334/1‑68 (MQ=255) cgcgcgcgTGAATCAGGTGCCGAGCGCATCATCGTGGCAACCGATCATGAGGATGTTGCCCGCGCCGtt < 1:3396382/69‑1 (MQ=255) gcgcgcgcGAATCAGGTGCCGAGCGCATCATCGTGGc < 1:112343/37‑1 (MQ=255) gcgcgcgTGAATCAGGTGCCGAGCGCATCATCGTGGCAACCGATCATGAGGATGTTGCCCGCGCCGTTg < 1:1366150/69‑1 (MQ=255) gcgcgcgTGAATCAGGTGCCGAGCGCATCATCGTGGCAACCGATCATGAGGATGTTGCCCGCGCCGTTg < 1:374865/69‑1 (MQ=255) gcgcgcgTGAATCAGGTGCCGAGCGCATCATCGTGGCAACCGATCATGAGGATGTTGCCCGCGCCGTTg < 1:2088929/69‑1 (MQ=255) gcgcgcgTGAATCAGGTGCCGAGCGCATCATCGTGGCAACCGATCATGAGGATGTTGCCCGCGCCGTTg < 1:248013/69‑1 (MQ=255) | TGCCCGGTAAACCATTGGTTGATATTAACGGCAAACCCATGATTGTTCATGTTCTTGAACGCGCGCGTGAATCAGGTGCCGAGCGCATCATCGTGGCAACCGATCATGAGGATGTTGCCCGCGCCGTTG > minE/627597‑627725 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |