| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 629,369 | A→G | 20.3% | R244R (CGT→CGC) | ycbC ← | conserved inner membrane protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 629,369 | 0 | A | G | 20.3% | 132.6 / 24.2 | 93 | R244R (CGT→CGC) | ycbC | conserved inner membrane protein |
| Reads supporting (aligned to +/- strand): ref base A (37/37); new base G (13/6); total (50/43) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.99e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
ACTTCGATCTGGCATCAAAATCACTCTTGCCGTGGCTCGCCTGACGAACCTTTTAGCCACTGCCAGATACGGCCAAGCGTTTCATAGCCAACACGATCGCTATGCATCAACCAGACCGGAGAAGGGATTGCCCGTT > minE/629301‑629436 | aCTTCGATCTGGCATCAAAATCACTCTTGCCGTGGCTCGCCTGACGAACCTTTTAGCCACTGCCAGATa < 1:2182639/69‑1 (MQ=255)aCTTCGATCTGGCATCAAAATCACTCTTGCCGTGGCTCGCCTGACGAACCTTTTAGCCACTGCCAGATa < 1:2288899/69‑1 (MQ=255)aCTTCGATCTGGCATCAAAATCACTCTTGCCGTGGCTCGCCTGACGAACCTTTTAGCCACTGCCAGATa < 1:1276436/69‑1 (MQ=255)aCTTCGATCTGGCATCAAAATCACTCTTGCCGTGGCTCGCCTGACGAACCTTTTAGCCACTGCCAGATa < 1:28032/69‑1 (MQ=255) cAAAATCACTCTTGCCGTGGCTCGCCTGACGAACCTTTTAGCCACTGCCAGATACGGCCAAGCGTTTCa < 1:744066/69‑1 (MQ=255) cAAAATCACTCTTGCCGTGGCTCGCCTGACGAACCTTTTAGCCACTGCCAGATACGGCCAAGCGTTTCa < 1:71167/69‑1 (MQ=255) cAAAATCACTCTTGCCGTGGCTCGCCTGACGAACCTTTTAGCCACTGCCAGATACGGCCAAGCGTTTCa < 1:561554/69‑1 (MQ=255) cAAAATCACTCTTGCCGTGGCTCGCCTGACGAACCTTTTAGCCACTGCCAGATACGGCCAAGCGTTTCa < 1:416298/69‑1 (MQ=255) cAAAATCACTCTTGCCGTGGCTCGCCTGACGAACCTTTTAGCCACTGCCAGATACGGCCAAGCGTTTCa < 1:2400923/69‑1 (MQ=255) cAAAATCACTCTTGCCGTGGCTCGCCTGACGAACCTTTTAGCCACTGCCAGATACGGCCAAGCGTTTCa < 1:2783789/69‑1 (MQ=255) cAAAATCACTCTTGCCGTGGCTCGCCTGACGAACCTTTTAGCCACTGCCAGATACGGCCAAGCGTTTCa < 1:2740701/69‑1 (MQ=255) cAAAATCACTCTTGCCGTGGCTCGCCTGACGAACCTTTTAGCCACTGCCAGATACGGCCAAGCGTTTCa < 1:1627011/69‑1 (MQ=255) tctTGCCGTGGCTCGCCTGACGAACCTTTTAGCCACTGCCAGATACGGCCAAGCGTTTCATAGCCAac < 1:2170428/68‑1 (MQ=255) tctTGCCGTGGCTCGCCTGACGAACCTTTTAGCCACTGCCAGATACGGCCAAGCGTTTCATAGCCAac < 1:1004172/68‑1 (MQ=255) tctTGCCGTGGCTCGCCTGACGAACCTTTTAGCCACTGCCAGATACGGCCAAGCGTTTCATAGCCAac < 1:2732175/68‑1 (MQ=255) tctTGCCGTGGCTCGCCTGACGAACCTTTTAGCCACTGCCAGATACGGCCAAGCGTTTCATAGCCAac < 1:2954232/68‑1 (MQ=255) tctTGCCGTGGCTCGCCTGACGAACCTTTTAGCCACTGCCAGATACGGCCAAGCGTTTCATAGCCAac < 1:3082854/68‑1 (MQ=255) tctTGCCGTGGCTCGCCTGACGAACCTTTTAGCCACTGCCAGATACGGCCAAGCGTTTCATAGCCAac < 1:3225804/68‑1 (MQ=255) tctTGCCGTGGCTCGCCTGACGAACCTTTTAGCCACTGCCAGATACGGCCAAGCGTTTCATAGCCAac < 1:429516/68‑1 (MQ=255) tctTGCCGTGGCTCGCCTGACGAACCTTTTAGCCACTGCCAGATACGGCCAAGCGTTTCATAGCCAac < 1:631547/68‑1 (MQ=255) tctTGCCGTGGCTCGCCTGACGAACCTTTTAGCCACTGCCAGATACGGCCAAGCGTTTCATAGCCAac < 1:158304/68‑1 (MQ=255) gCCGTGGCTCGCCTGACGAACCTTTTAGCCACTGCCAGATACGGCCAAGCGTTTCATAGCCAACACg < 1:2605901/67‑1 (MQ=255) gCCGTGGCTCGCCTGACGAACCTTTTAGCCACTGCCAGATACGGCCAAGCGTTTCATAGCCAACACg < 1:2207764/67‑1 (MQ=255) gTGGCTCGCCTGACGAACCTTTTAGCCACTGCCAGATACGGCCAAGCGTTTCATAGCCAACACGATCGc < 1:487881/69‑1 (MQ=255) gTGGCTCGCCTGACGAACCTTTTAGCCACTGCCAGATACGGCCAAGCGTTTCATAGCCAACACGATCGc < 1:1480363/69‑1 (MQ=255) gTGGCTCGCCTGACGAACCTTTTAGCCACTGCCAGATACGGCCAAGCGTTTCATAGCCAACACGATCGc < 1:3027408/69‑1 (MQ=255) gTGGCTCGCCTGACGAACCTTTTAGCCACTGCCAGATACGGCCAAGCGTTTCATAGCCAACACGATCGc < 1:2276959/69‑1 (MQ=255) gTGGCTCGCCTGACGAACCTTTTAGCCACTGCCAGATACGGCCAAGCGTTTCATAGCCAACACGATCGc < 1:1826483/69‑1 (MQ=255) gTGGCTCGCCTGACGAACCTTTTAGCCACTGCCAGATACGGCCAAGCGTTTCATAGCCAACACGATCGc < 1:2120044/69‑1 (MQ=255) gTGGCTCGCCTGACGAACCTTTTAGCCACTGCCAGATACGGCCAAGCGTTTCATAGCCAACACGATCGc < 1:1051067/69‑1 (MQ=255) gTGGCTCGCCTGACGAACCTTTTAGCCACTGCCAGATACGGCCAAGCGTTTCATAGCCAACACGATCGc < 1:722305/69‑1 (MQ=255) gTGGCTCGCCTGACGAACCTTTTAGCCACTGCCAGATACGGCCAAGCGTTTCATAGCCAACACGATCGc < 1:1899667/69‑1 (MQ=255) gTGGCTCGCCTGACGAACCTTTCAGCCACTGCCAGATACGGCCAAGCGTTTCATAGCCAACACGATCGc < 1:1154893/69‑1 (MQ=255) gCTCGCCTGACGAACCTTTTAGCCACTGCCAGATACGGCCAAGCg > 1:536683/1‑45 (MQ=255) tCGCCTGACGAACCTTTTAGCCACTGCCAGATACGGCCAAGCGTTTCATAGCCAACACGATCGCTATg < 1:2338384/68‑1 (MQ=255) cTTTTAGCCACTGCCAGATGCGGCCAAGCGTTTCATAGCCAACACGATCGCTATGCATCAACCAGa > 1:2263790/1‑66 (MQ=255) cTTTTAGCCACTGCCAGATGCGGCCAAGCGTTTCATAGCCAACACGATCGCTATGCATCAACCAGAc > 1:628296/1‑67 (MQ=255) cTTTTAGCCACTGCCAGATGCGGCCAAGCGTTTCATAGCCAACACGATCGCTATGCATCAACCAGAc > 1:3053446/1‑67 (MQ=255) cTTTTAGCCACTGCCAGATGCGGCCAAGCGTTTCATAGCCAACACGATCGCTATGCATCAACCAGAc > 1:660845/1‑67 (MQ=255) cTTTTAGCCACTGCCAGATGCGGCCAAGCGTTTCATAGCCAACACGATCGCTATGCATCAACCAGAc > 1:2858538/1‑67 (MQ=255) cTTTTAGCCACTGCCAGATGCGGCCAAGCGTTTCATAGCCAACACGATCGCTATGCATCAACCAGAc > 1:1002906/1‑67 (MQ=255) cTTTTAGCCACTGCCAGATGCGGCCAAGCGTTTCATAGCCAACACGATCGCTATGCATCAACCAGAc > 1:1704941/1‑67 (MQ=255) cTTTTAGCCACTGCCAGATGCGGCCAAGCGTTTCATAGCCAACACGATCGCTATGCATCAACCAGAc > 1:261099/1‑67 (MQ=255) cTTTTAGCCACTGCCAGATGCGGCCAAGCGTTTCATAGCCAACACGATCGCTATGCATCAACCAGAc > 1:2258688/1‑67 (MQ=255) cTTTTAGCCACTGCCAGATGCGGCCAAGCGTTTCATAGCCAACACGATCGCTATGCATCAACCAGAc > 1:1768995/1‑67 (MQ=255) cTTTTAGCCACTGCCAGATGCGGCCAAGCGTTTCATAGCCAACACGATCGCTATGCATCAACCAGAc > 1:2078303/1‑67 (MQ=255) cTTTTAGCCACTGCCAGATGCGGCCAAGCGATTCATAGCCAACACGATCGCTATGCATCAACCAGAc > 1:655516/1‑67 (MQ=255) cTTTTAGCCACTGCCAGATGCGGCCAAGAGTTTCATAGCCAACACGATCGCTATGCATCAACCAGAc > 1:671098/1‑67 (MQ=255) tttAGCCACTGCCAGATACGTCCAAGCGTTTCATAGCCAACACg > 1:2713105/1‑44 (MQ=255) tttAGCCACTGCCAGATACGGCCAAGCGTTTCATa > 1:675601/1‑35 (MQ=255) tttAGCCACTGCCAGATACGGCCAAGCGTTTCATAGCCAACACg > 1:3317908/1‑44 (MQ=255) tttAGCCACTGCCAGATACGGCCAAGCGTTTCATAGCCAACACg > 1:2108165/1‑44 (MQ=255) tttAGCCACTGCCAGATACGGCCAAGCGTTTCATAGCCAACACg > 1:1255571/1‑44 (MQ=255) tttAGCCACTGCCAGATACGGCCAAGCGTTTCATAGCCAACACg > 1:3317686/1‑44 (MQ=255) tttAGCCACTGCCAGATACGGCCAAGCGTTTCATAGCCAACACg > 1:521042/1‑44 (MQ=255) tttAGCCACTGCCAGATACGGCCAAGCGTTTCATAGCCAACACg > 1:617668/1‑44 (MQ=255) tttAGCCACTGCCAGATACGGCCAAGCGTTTCATAGCCAACACg > 1:594752/1‑44 (MQ=255) tttAGCCACTGCCAGATACGGCCAAGCGTTTCATAGCCAACACg > 1:228555/1‑44 (MQ=255) tttAGCCACTGCCAGATACGGCCAAGCGTTTCATAGCCAACACg > 1:455383/1‑44 (MQ=255) tttAGCCACTGCCAGATACGGCCAAGCGTTTCATAGCCAACACg > 1:58529/1‑44 (MQ=255) tttAGCCACTGCCAGATACGGCCAAGCGTTTCATAGCCAACACg > 1:646609/1‑44 (MQ=255) tttAGCCACTGCCAGATACGGCCAAGCGTTTCATAGCCAACACg > 1:650556/1‑44 (MQ=255) tttAGCCACTGCCAGATACGGCCAAGCGTTTCATAGCCAACACg > 1:1896411/1‑44 (MQ=255) tttAGCCACTGCCAGATACGGCCAAGCGTTTCATAGCCAACACg > 1:1392931/1‑44 (MQ=255) tttAGCCACTGCCAGATACGGCCAAGCGTTTCATAGCCAACACg > 1:679532/1‑44 (MQ=255) tttAGCCACTGCCAGATACGGCCAAGCGTTTCATAGCCAACACg > 1:276318/1‑44 (MQ=255) tttAGCCACTGCCAGATACGGCCAAGCGTTTCATAGCCAACACg > 1:2449803/1‑44 (MQ=255) tttAGCCACTGCCAGATACGGCCAAGCGTTTCATAGCCAACACg > 1:1937835/1‑44 (MQ=255) tttAGCCACTGCCAGATACGGCCAAGCGTTTCATAGCCAACACg > 1:2680198/1‑44 (MQ=255) tttAGCCACTGCCAGATACGGCCAAGCGTTTCATAGCCAACACg > 1:930597/1‑44 (MQ=255) aGCCACTGCCAGATGCGGCCAAGCGTTTCATAGCCAACACGATCGCTATGCATCAACCAGACCGgaga < 1:860461/68‑1 (MQ=255) aGCCACTGCCAGATGCGGCCAAGCGTTTCATAGCCAACACGATCGCTATGCATCAACCAGACCGgaga < 1:49036/68‑1 (MQ=255) aGCCACTGCCAGATGCGGCCAAGCGTTTCATAGCCAACACGATCGCTATGCATCAACCAGACCGgaga < 1:1292662/68‑1 (MQ=255) aGCCACTGCCAGATGCGGCCAAGCGTTTCATAGCCAACACGATCGCTATGCATCAACCAGACCGgaga < 1:2639990/68‑1 (MQ=255) aGCCACTGCCAGATGCGGCCAAGCGTTTCATAGCCAACACGATCGCTATGCATCAACCAGACCGgaga < 1:1738203/68‑1 (MQ=255) aGCCACTGCCAGATGCGGCCAAGCGTTTCATAGCCAACACGATAGCTATGCATCAACCAGACCGgaga < 1:1035910/68‑1 (MQ=255) cACTGCCAGATACGGCCAAGCGTTTCATAGCCAACACGATCGCTATGCATCAACCAGACCGGAGAAggg < 1:920602/69‑1 (MQ=255) cACTGCCAGATACGGCCAAGCGTTTCATAGCCAACACGATCGCTATGCATCAACCAGACCGGAGAAggg < 1:2670249/69‑1 (MQ=255) cACTGCCAGATACGGCCAAGCGTTTCATAGCCAACACGATCGCTATGCATCAACCAGACCGGAGAAggg < 1:579014/69‑1 (MQ=255) cACTGCCAGATACGGCCAAGCGTTTCATAGCCAACACGATCGCTATGCATCAACCAGACCGGAGAAggg < 1:3163553/69‑1 (MQ=255) gCCAGATACGGCCAAGCGTTTCATAGCCAACACGATCGCTATGCATCAACCAGACCGGAGAAGGGATTg > 1:1086469/1‑69 (MQ=255) gCCAGATACGGCCAAGCGTTTCATAGCCAACACGATCGCTATGCATCAACCAGACCGGAGAAGGGATTg > 1:540533/1‑69 (MQ=255) gCCAGATACGGCCAAGCGTTTCATAGCCAACACGATCGCTATGCATCAACCAGACCGGAGAAGGGATTg > 1:2456768/1‑69 (MQ=255) gCCAGATACGGCCAAGCGTTTCATAGCCAACACGATCGCTATGCATCAACCAGACCGGAGAAGGGATTg > 1:2447323/1‑69 (MQ=255) gCCAGATACGGCCAAGCGTTTCATAGCCAACACGATCGCTATGCATCAACCAGACCGGAGAAGGGATTg > 1:3024981/1‑69 (MQ=255) gCCAGATACGGCCAAGCGTTTCATAGCCAACACGATCGCTATGCATCAACCAGACCGGAGAAGGGATTg > 1:1814041/1‑69 (MQ=255) gCCAGATACGGCCAAGCGTTTCATAGCCAACACGATCGCTATGCATCAACCAGACCGGAGAAGGGATTg > 1:2148278/1‑69 (MQ=255) gCCAGATACGGCCAAGCGTTTCATAGCCAACACGATCGCTATGCATCAACCAGACCGGAGAAGGGATTg > 1:363961/1‑69 (MQ=255) gCCAGATACGGCCAAGCGTTTCATAGCCAACACGATCGCTATGCATCAACCAGACCGGAGAAGGGATTg > 1:2503589/1‑69 (MQ=255) gCCAGATACGGCCAAGCGTTTCAAAGCCAACACGATCGCTATGCATCAACCAGACCGGAGAAGGGATTg > 1:590696/1‑69 (MQ=255) aGATACGGCCAAGCGTTTCATAGCCAACACGATCGCTATGCATCAACCAGACCGGAGAAGGGATTGcc < 1:761325/68‑1 (MQ=255) aGATACGGCCAAGCGTTTCATAGCCAACACGATCGCTATGCATCAACCAGACCGGAGAAGGGATTGcc < 1:1215372/68‑1 (MQ=255) aGATACGGCCAAGCGTTTCATAGCCAACACGATCGCTATGCATAAACCAGACCGGAGAAGGGATTGcc < 1:3170608/68‑1 (MQ=255) tACGGCCAAGCGTTTCATAGCCAACACGATCGCTATGCATCAACCAGACCGGAGAAGGGATTGCCCGtt > 1:2817428/1‑69 (MQ=255) tACGGCCAAGCGTTTCATAGCCAACACGATCGCTATGCATCAACCAGACCGGAGAAGGGATTGCCCGtt > 1:626456/1‑69 (MQ=255) tACGGCCAAGCGTTTCATAGCCAACACGATCGCTATGCATCAACCAGACCGGAGAAGGGATTGCCCGtt > 1:2068678/1‑69 (MQ=255) tACGGCCAAGCGTTTCATAGCCAACACGATCGCTATGCATCAACCAGACCGGAGAAGGGATTGCCAGtt > 1:1425107/1‑69 (MQ=255) | ACTTCGATCTGGCATCAAAATCACTCTTGCCGTGGCTCGCCTGACGAACCTTTTAGCCACTGCCAGATACGGCCAAGCGTTTCATAGCCAACACGATCGCTATGCATCAACCAGACCGGAGAAGGGATTGCCCGTT > minE/629301‑629436 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |