| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 632,854 | T→C | 41.0% | F169F (TTT→TTC) | mukE → | protein involved in chromosome partitioning |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 632,854 | 0 | T | C | 41.0% | 26.4 / 56.2 | 78 | F169F (TTT→TTC) | mukE | protein involved in chromosome partitioning |
| Reads supporting (aligned to +/- strand): ref base T (21/25); new base C (5/27); total (26/52) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 7.16e-03 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
TAGGCATGGTGTGGTTTATGGGCCACGACAGCAGCAAGTTTCGCATTACCGAATCGGTGTTCCGCTTCGGAGCCGATGTGCGTGCTGGCGACGATCCCCGTGAAGCAC > minE/632814‑632921 | tAGGCATGGTGTGGTTTATGGGCCACGACAGCAGCAAGTTTCGCATTACCGAATCGGTGTTCCGCtt > 1:1011298/1‑67 (MQ=255)tAGGCATGGTGTGGTTTATGGGCCACGACAGCAGCAAGTTTCGCATTACCGAATCGGTGTTCCGCtt > 1:803705/1‑67 (MQ=255)tAGGCATGGTGTGGTTTATGGGCCACGACAGCAGCAAGTTTCGCATTACCGAATCGGTGTTCCGCtt > 1:2546331/1‑67 (MQ=255)tAGGCATGGTGTGGTTTATGGGCCACGACAGCAGCAAGTTTCGCATTACCGAATCGGTGTTCCGCtt > 1:2500510/1‑67 (MQ=255)tAGGCATGGTGTGGTTTATGGGCCACGACAGCAGCAAGTTTCGCATTACCGAATCGGTGTTCCGCtt > 1:2432950/1‑67 (MQ=255)tAGGCATGGTGTGGTTTATGGGCCACGACAGCAGCAAGTTTCGCATTACCGAATCGGTGTTCCGCtt > 1:1005437/1‑67 (MQ=255)tAGGCATGGTGTGGTTTATGGGCCACGACAGCAGCAAGTTTCGCATTACCGAATCGGTGTTCCGCtt > 1:3338399/1‑67 (MQ=255)tAGGCATGGTGTGGTTTATGGGCCACGACAGCAGCAAGTTTCGCATTACCGAATCGGTGTTCCGCtt > 1:316993/1‑67 (MQ=255)tAGGCATGGTGTGGTTTATGGGCCACGACAGCAGCAAGTTTCGCATTACCGAATCGGTGTTCCGCtt > 1:134969/1‑67 (MQ=255) gtgtGGTTTATGGGCGACGACAGCAGCAAGTTTCGCATTACCGAATCGGTGTTCCGCTTCGGAGCCGAt > 1:1775584/1‑69 (MQ=255) gtgtGGTTTATGGGCCACGACAGCAGCAAGTTTCGCATTACCGAATCGGTGTTCCGCTTCGGAGCCGAt > 1:1857781/1‑69 (MQ=255) gtgtGGTTTATGGGCCACGACAGCAGCAAGTTTCGCATTACCGAATCGGTGTTCCGCTTCGGAGCCGAt > 1:2949737/1‑69 (MQ=255) gtgtGGTTTATGGGCCACGACAGCAGCAAGTTTCGCATTACCGAATCGGTGTTCCGCTTCGGAGCCGAt > 1:166842/1‑69 (MQ=255) gtgtGGTTTATGGGCCACGACAGCAGCAAGTTTCGCATTACCGAATCGGTGTTCCGCTTCGGAGCCGAt > 1:1312945/1‑69 (MQ=255) gtgtGGTTTATGGGCCACGACAGCAGCAAGTTCCGCATTACCGAATCGGTGTTCCGCTTCGGAGCCGAt > 1:776728/1‑69 (MQ=255) gtgtGGTTTATGGGCCACGACAGCAGCAAGTTCCGCATTACCGAATCGGTGTTCCGCTTCGGAGCCGAt > 1:2791634/1‑69 (MQ=255) gtgtGGTTTATGGGCCACGACAGCAGCAAGTTCCGCATTACCGAATCGGTGTTCCGCTTCGGAGCCGAt > 1:1231713/1‑69 (MQ=255) gtgtGGTTTATGGGCCACGACAGCAGCAAGTTCCGCATTACCGAATCGGTGTTCCGCTTCGGAGCCGAt > 1:690106/1‑69 (MQ=255) gtgtGGTTTATGGGCCACGACAGCAGCAAGTACCGCATTACCGAATCGGTCTTc > 1:935089/1‑54 (MQ=255) gtgGTTTATGGGCCACGACAGCAGCAAGTTTCGCATTACCGAATCGGTGTTCCGCTTCGGAGCCGAtgt > 1:3259947/1‑69 (MQ=255) gtgGTTTATGGGCCACGACAGCAGCAAGTTTCGCATTACCGAATCGGTGTTCCGCTTCGGAGCCGAtgt > 1:20679/1‑69 (MQ=255) gtgGTTTATGGGCCACGACAGCAGCAAGTTTCGCATTACCGAATCGGTGTTCCGCTTCGGAGCCGAtgt > 1:1882731/1‑69 (MQ=255) gtgGTTTATGGGCCACGACAGCAGCAAGTTTCGCATTACCGAATCGGTGTTCCGCTTCGGAGCCGAtgt > 1:3128719/1‑69 (MQ=255) gtgGTTTATGGGCCACGACAGCAGCAAGTTTCGCATTACCGAATCGGTGTTCCGCTTCGGAGCCGAtgt > 1:656576/1‑69 (MQ=255) gtgGTTTATGGGCCACGACAGCAGCAAGTTTCGCATTACCGAATCGGTGTTCCGCTTCGGAGCCGAtgt > 1:662130/1‑69 (MQ=255) gtgGTTTATGGGCCACGACAGCAGCAAGTTTCGCATTACCGAATCGGTGTTCCGCTTCGGAGCCGAtgt > 1:1435583/1‑69 (MQ=255) gtgGTTTATGGGCCACGACAGCAGCAAGTTTCGCATTACCGAATCGGTGTTCCGCTTCGGAGCCGAtg < 1:1707788/68‑1 (MQ=255) tgGTTTATGGGCCACGACAGCAGCAAGTTTCGCATTACCGAATCGGTGTTCCGCTTCGGAGCCGAtgtg < 1:3436329/69‑1 (MQ=255) tgGTTTATGGGCCACGACAGCAGCAAGTTTCGCATTACCGAATCGGTGTTCCGCTTCGGAGCCGAtgtg < 1:267257/69‑1 (MQ=255) tgGTTTATGGGCCACGACAGCAGCAAGTTTCGCATTACCGAATCGGTGTTCCGCTTCGGAGCCGAtgtg < 1:1627230/69‑1 (MQ=255) ttATGGTCCACGACAGCAGCAAGTTTCGCATTACCGAATc < 1:2606427/40‑1 (MQ=255) ttATGGGCCACGACAGCAGCAAGTTTCGCATTACCGAATc < 1:2378738/40‑1 (MQ=255) ttATGGGCCACGACAGCAGCAAGTTTCGCATTACCGAATc < 1:1083539/40‑1 (MQ=255) ttATGGGCCACGACAGCAGCAAGTTTCGCATTACCGAATc < 1:2546463/40‑1 (MQ=255) ttATGGGCCACGACAGCAGCAAGTTTCGCATTACCGAATc < 1:951617/40‑1 (MQ=255) ttATGGGCCACGACAGCAGCAAGTTTCGCATTACCGAATc < 1:3365140/40‑1 (MQ=255) ttATGGGCCACGACAGCAGCAAGTTTCGCATTACCGAATc < 1:2043800/40‑1 (MQ=255) ttATGGGCCACGACAGCAGCAAGTTTCGCATTACCGAATc < 1:1529630/40‑1 (MQ=255) ttATGGGCCACGACAGCAGCAAGTTTCGCATTACCGAATc < 1:522343/40‑1 (MQ=255) tATGGGCCACGTCAGCAGCAAGTTCCGCATTACCGAATCg < 1:2253076/40‑1 (MQ=38) tATGGGCCACGACATCAGCAAGTTCCGCATTACCGAATCg < 1:1309213/40‑1 (MQ=38) tATGGGCCACGACAGCAGCAAGTTTCGCATTACCGAATc < 1:414131/39‑1 (MQ=255) tATGGGCCACGACAGCAGCAAGTTCCGCATTACCGAATCg < 1:431534/40‑1 (MQ=255) tATGGGCCACGACAGCAGCAAGTTCCGCATTACCGAATCg < 1:386965/40‑1 (MQ=255) tATGGGCCACGACAGCAGCAAGTTCCGCATTACCGAATCg < 1:2726229/40‑1 (MQ=255) tATGGGCCACGACAGCAGCAAGTTCCGCATTACCGAATCg < 1:347858/40‑1 (MQ=255) tATGGGCCACGACAGCAGCAAGTTCCGCATTACCGAATCg < 1:2986947/40‑1 (MQ=255) tATGGGCCACGACAGCAGCAAGTTCCGCATTACCGAATCg < 1:3045458/40‑1 (MQ=255) tATGGGCCACGACAGCAGCAAGTTCCGCATTACCGAATCg < 1:411529/40‑1 (MQ=255) tATGGGCCACGACAGCAGCAAGTTCCGCATTACCGAATCg < 1:310846/40‑1 (MQ=255) tATGGGCCACGACAGCAGCAAGTTCCGCATTACCGAATCg < 1:3185135/40‑1 (MQ=255) tATGGGCCACGACAGCAGCAAGTTCCGCATTACCGAATCg < 1:3231580/40‑1 (MQ=255) tATGGGCCACGACAGCAGCAAGTTCCGCATTACCGAATCg < 1:71980/40‑1 (MQ=255) tATGGGCCACGACAGCAGCAAGTTCCGCATTACCGAATCg < 1:536927/40‑1 (MQ=255) tATGGGCCACGACAGCAGCAAGTTCCGCATTACCGAATCg < 1:1108918/40‑1 (MQ=255) tATGGGCCACGACAGCAGCAAGTTCCGCATTACCGAATCg < 1:1254608/40‑1 (MQ=255) tATGGGCCACGACAGCAGCAAGTTCCGCATTACCGAATCg < 1:1337923/40‑1 (MQ=255) tATGGGCCACGACAGCAGCAAGTTCCGCATTACCGAATCg < 1:1365456/40‑1 (MQ=255) tATGGGCCACGACAGCAGCAAGTTCCGCATTACCGAATCg < 1:1439458/40‑1 (MQ=255) tATGGGCCACGACAGCAGCAAGTTCCGCATTACCGAATCg < 1:1589822/40‑1 (MQ=255) tATGGGCCACGACAGCAGCAAGTTCCGCATTACCGAATCg < 1:1765329/40‑1 (MQ=255) tATGGGCCACGACAGCAGCAAGTTCCGCATTACCGAATCg < 1:1835459/40‑1 (MQ=255) tATGGGCCACGACAGCAGCAAGTTCCGCATTACCGAATCg < 1:1856295/40‑1 (MQ=255) tATGGGCCACGACAGCAGCAAGTTCCGCATTACCGAATCg < 1:2213013/40‑1 (MQ=255) tATGGGCCACGACAGCAGCAAGTTCCGCATTACCGAATCg < 1:2281150/40‑1 (MQ=255) tATGGGCCACGACAGCAGCAAGTTCCGCATTACCGAATCg < 1:2356098/40‑1 (MQ=255) tATGGGCCACGACAGCAGCAAGTTCCGCATTACCGAATCg < 1:2696813/40‑1 (MQ=255) gcaAGTTTCGCATTACCGAATCTGTGTTCCGCTTCGGAGCCGATGTGCGTGCTGGCGACGATCCCCGTg < 1:1240389/69‑1 (MQ=255) gcaAGTTTCGCATTACCGAATCGGTGTTCCGCTTCGGAGCCGATGTGCGTGCTGGCGACGATCCCCGTg < 1:2337339/69‑1 (MQ=255) gcaAGTTTCGCATTACCGAATCGGTGTTCCGCTTCGGAGCCGATGTGCGTGCTGGCGACGATCCCCGTg < 1:2541418/69‑1 (MQ=255) gcaAGTTTCGCATTACCGAATCGGTGTTCCGCTTCGGAGCCGATGTGCGTGCTGGCGACGATCCCCGTg < 1:26062/69‑1 (MQ=255) gcaAGTTTCGCATTACCGAATCGGTGTTCCGCTTCGGAGCCGATGTGCGTGCTGGCGACGATCCCCGTg < 1:1042119/69‑1 (MQ=255) aGTTTCGCATTACCGAATCGGTGTTCCGCTTCGGAGCCGATGTGCGTGCTGGCGACGATCCCCGTGAAg < 1:2453463/69‑1 (MQ=255) aGTTTCGCATTACCGAATCGGTGTTCCGCTTCGGAGCCGATGTGCGTGCTGGCGACGATCCCCGTGAAg < 1:2961423/69‑1 (MQ=255) aGTTTCGCATTACCGAATCGGTGTTCCGCTTCGGAGCCGATGTGCGTGCTGGCGACGATCCCCGTGAAg < 1:2921630/69‑1 (MQ=255) aGTTTCGCATTACCGAATCGGTGTTCCGCTTCGGAGCCGATGTGCGTGCTGGCGACGATCCCCGTGAAg < 1:2752101/69‑1 (MQ=255) aGTTTCGCATTACCGAATCGGTGTTCCGCTTCGGAGCCGATGTGCGTGCTGGCGACGATCCCCGTGAAg < 1:1351640/69‑1 (MQ=255) aGTTTCGCATTACCGAATCGGTGTTCCGCTTCGGAGCCGATGTGCGTGCTGGCGACGATCCCCGTGAAg < 1:1070717/69‑1 (MQ=255) tttCGCATTACCGAATCGGTGTTCCGCTTCGGAGCCGATGTGCGTGCTGGCGACGATCCCCGTGAAGca < 1:972308/69‑1 (MQ=255) tttCGCATTACCGAATCGGTGTTCCGCTTCGGAGCCGATGTGCGTGCTGGCGACGATCCCCGTGAAGca < 1:622093/69‑1 (MQ=255) tttCGCATTACCGAATCGGTGTTCCGCTTCGGAGCCGATGTGCGTGCTGGCGACGATCCCCGTGAAGca < 1:48717/69‑1 (MQ=255) tttCGCATTACCGAATCGGTGTTCCGCTTCGGAGCCGATGTGCGTGCTGGCGACGATCCCCGTGAAGca < 1:431479/69‑1 (MQ=255) tttCGCATTACCGAATCGGTGTTCCGCTTCGGAGCCGATGTGCGTGCTGGCGACGATCCCCGTGAAGca < 1:2577287/69‑1 (MQ=255) tttCGCATTACCGAATCGGTGTTCCGCTTCGGAGCCGATGTGCGTGCTGGCGACGATCCCCGTGAAGca < 1:2063557/69‑1 (MQ=255) ttCGCGTTACCGAATCGGTGTTCCGCTTCGGAGCCGATGTGCGTGCTGGCGACGATCCCCGTGAAGcac > 1:2673677/1‑69 (MQ=255) ttCGCATTACCGAATCGGTGTTCCGCTTCGGAGCCGATGTGCGTGCTGGCGACGATCCCCGTGAAGcac > 1:598340/1‑69 (MQ=255) ttCGCATTACCGAATCGGTGTTCCGCTTCGGAGCCGATGTGCGTGCTGGCGACGATCCCCGTGAAGcac > 1:3323207/1‑69 (MQ=255) ttCGCATTACCGAATCGGTGTTCCGCTTCGGAGCCGATGTGCGTGCTGGCGACGATCCCCGTGAAGcac > 1:706097/1‑69 (MQ=255) ttCGCATTACCGAATCGGTGTTCCGCTTCGGAGCCGATGTGCGTGCTGGCGACGATCCCCGTGAAGcac > 1:25701/1‑69 (MQ=255) ttCGCATTACCGAATCGGTGTTCCGCTTCGGAGCCGATGTGCGTGCTGGCGACGATCCCCGTGAAGcac > 1:2335898/1‑69 (MQ=255) ttCGCATTACCGAATCGGTGTTCCGCTTCGGAGCCGATGTGCGTGCTGGCGACGATCCCCGTGAAGcac > 1:2129955/1‑69 (MQ=255) | TAGGCATGGTGTGGTTTATGGGCCACGACAGCAGCAAGTTTCGCATTACCGAATCGGTGTTCCGCTTCGGAGCCGATGTGCGTGCTGGCGACGATCCCCGTGAAGCAC > minE/632814‑632921 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |