| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 634,587 | A→C | 42.9% | R521R (CGA→CGC) | mukB → | fused chromosome partitioning proteins |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 634,587 | 0 | A | C | 42.9% | 10.1 / 67.2 | 63 | R521R (CGA→CGC) | mukB | fused chromosome partitioning proteins |
| Reads supporting (aligned to +/- strand): ref base A (20/16); new base C (4/23); total (24/39) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.45e-03 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
TTGCGCGAAGGGGTCGATCAGCGTCACCTGGCAGAGCAGGTTCAGCCGCTGCGGATGCGATTAAGCGAACTGGAACAGCGTCTACGCGAGCAGCAAGAAGCTGAGCGTCTGCTGGCA > minE/634528‑634644 | ttGCGCGAAGGGGTCGATCAGCGTCACCTGGCAGAGCAGGTTCAGCCGCTGCGGATGCGATTAAGCGaa > 1:2501500/1‑69 (MQ=255)ttGCGCGAAGGGGTCGATCAGCGTCACCTGGCAGAGCAGGTTCAGCCGCTGCGGATGCGATTAAGCGaa > 1:3146750/1‑69 (MQ=255)ttGCGCGAAGGGGTCGATCAGCGTCACCTGGCAGAGCAGGTTCAGCCGCTGCGGATGCGATTAAGCGaa > 1:1747045/1‑69 (MQ=255)ttGCGCGAAGGGGTCGATCAGCGTCACCTGGCAGAGCAGGTTCAGCCGCTGCGGATGCGATTAAGCGaa > 1:45615/1‑69 (MQ=255)ttGCGCGAAGGGGTCGATCAGCGTCACCTGGCAGAGCAGGTTCAGCCGCTGCGGATGCGATTAAGCGaa > 1:1479720/1‑69 (MQ=255)ttGCGCGAAGGGGTCGATCAGCGTCACCTGGCAGAGCAGGTTCAGCCGCTGCGGATGCGATTAAGCGaa > 1:2299641/1‑69 (MQ=255)ttGCGCGAAGGGGTCGATCAGCGTCACCTGGCAGAGCAGGTTCAGCCGCTGCGGATGCGATTAAGCGaa > 1:1119803/1‑69 (MQ=255)ttGCGCGAAGGGGTCGATCAGCGTCACCTGGCAGAGCAGGTTCAGCCGCTGCGGATGCGATTAAGCGa > 1:3230675/1‑68 (MQ=255)ttGCGAGAAGGGGTCGATCAGCGTCACCTGGCAGAGCAGGTTCAGCCGCTGCGGATGCGATTAAGCGaa > 1:3039307/1‑69 (MQ=255) tGCGCGAAGGGGTCGATCAGCGTCACCTGGCAGAGCAGGTTCAGCCGCTGCGGATGCGATTAAGCg > 1:789670/1‑66 (MQ=255) cgAAGGGGTCGATCAGCGTCACCTGGCATAGCAGGTTCAGCCGCTGCGGATGCGATTAAGCGAACTgg < 1:1279710/68‑1 (MQ=255) cgAAGGGGTCGATCAGCGTCACCTGGCAGAGCAGGTTCAGCCGCTGCGGATGCGATTAAGCGAACTgg < 1:2266450/68‑1 (MQ=255) cgAAGGGGTCGATCAGCGTCACCTGGCAGAGCAGGTTCAGCCGCTGCGGATGCGATTAAGCGAACTgg < 1:111115/68‑1 (MQ=255) cgAAGGGGTCGATCAGCGTCACCTGGCAGAGCAGGTTCAGCCGCTGCGGATGCGATTAAGCGAACTgg < 1:1894456/68‑1 (MQ=255) cgAAGGGGTCGATCAGCGTCACCTGGCAGAGCAGGTTCAGCCGCTGCGGATGCCATTAAGCGAACTgg < 1:3376759/68‑1 (MQ=255) gggTCGATCAGCGTCACCTGGCAGAGCAGGTTCAGCCGCTGCGGATGCGATTAAGCGAACTGGAACAGc > 1:2816144/1‑69 (MQ=255) gTCGATCAGCGTCACCTGGCAGAGCAGGTTCAGCCGTTGCGGATGCGCTTAAGCGAACTGGAACAGCGt < 1:2735088/69‑1 (MQ=255) gTCGATCAGCGTCACCTGGCAGAGCAGGTTCAGCCGTTGCGGATGCGCTTAAGCGAACTGGAACAGCGt < 1:3403309/69‑1 (MQ=255) gTCGATCAGCGTCACCTGGCAGAGCAGGTTCAGCCGTTGCGGATGCGCTTAAGCGAACTGGAACAGCGt < 1:2918566/69‑1 (MQ=255) gTCGATCAGCGTCACCTGGCAGAGCAGGTTCAGCCGTTGCGGATGCGCTTAAGCGAACTGGAACAGCGt < 1:2976428/69‑1 (MQ=255) gTCGATCAGCGTCACCTGGCAGAGCAGGTTCAGCCGTTGCGGATGCGCTTAAGCGAACTGGAACAGCGt < 1:1015414/69‑1 (MQ=255) gTCGATCAGCGTCACCTGGCAGAGCAGGTTCAGCCGTTGCGGATGCGCTTAAGCGAACTGGAACAGCGt < 1:621023/69‑1 (MQ=255) gTCGATCAGCGTCACCTGGCAGAGCAGGTTCAGCCGTTGCGGATGCGCTTAAGCGAACTGGAACAGCGt < 1:623343/69‑1 (MQ=255) gTCGATCAGCGTCACCTGGCAGAGCAGGTTCAGCCGTTGCGGATGCGCTTAAGCGAACTGGAACAGCGt < 1:633206/69‑1 (MQ=255) gTCGATCAGCGTCACCTGGCAGAGCAGGTTCAGCCGTTGCGGATGCGCTTAAGCGAACTGGAACAGCGt < 1:981558/69‑1 (MQ=255) gTCGATCAGCGTCACCTGGCAGAGCAGGTTCAGCCGTTGCGGATGCGCTTAAGCGAACTGGAACAGCGt < 1:1867107/69‑1 (MQ=255) gTCGATCAGCGTCACCTGGCAGAGCAGGTTCAGCCGTTGCGGATGCGCTTAAGCGAACTGGAACAGCGt < 1:1003077/69‑1 (MQ=255) gTCGATCAGCGTCACCTGGCAGAGCAGGTTCAGCCGTTGCGGATGCGCTTAAGCGAACTGGAACAGCGt < 1:1362912/69‑1 (MQ=255) gTCGATCAGCGTCACCTGGCAGAGCAGGTTCAGCCGTTGCGGATGCGCTTAAGCGAACTGGAACAGCGt < 1:1716397/69‑1 (MQ=255) gTCGATCAGCGTCACCTGGCAGAGCAGGTTCAGCCGTTGCGGATGCGCTTAAGCGAACTGGAACAGCGt < 1:1794336/69‑1 (MQ=255) gTCGATCAGCGTCACCTGGCAGAGCAGGTTCAGCCGTTGCGGATGCGCTTAAGCGAACTGGAACAGCGt < 1:1982910/69‑1 (MQ=255) gTCGATCAGCGTCACCTGGCAGAGCAGGTTCAGCCGTTGCGGATGCGCTTAAGCGAACTGGAACAGCGt < 1:2105047/69‑1 (MQ=255) gTCGATCAGCGTCACCTGGCAGAGCAGGTTCAGCCGTTGCGGATGCGCTTAAGCGAACTGGAACAGCGt < 1:2230329/69‑1 (MQ=255) gTCGATCAGCGTCACCTGGCAGAGCAGGTTCAGCCGTTGCGGATGCGCTTAAGCGAACTGGAACAGCGt < 1:235974/69‑1 (MQ=255) tCGATCAGCGTCACCTGGCAGAGCAGGTTCAGCCGTTGCGGATGCGCTTAAGCGAACTGGAACAGCGt < 1:461175/68‑1 (MQ=255) tCGATCAGCGTCACCTGGCAGAGCAGGTTCAGCCGTTGCGGATGCGCTTAAGCGAACTGGAACAGCGt < 1:2434888/68‑1 (MQ=255) cGTCACCTGGCAGAGCAGGTTCAGCCGTTGCGGATGCGCTTAAGCGAACTGGAACAGCGt < 1:264855/60‑1 (MQ=255) tCACCTGGCAGAGCAGGTTCAGCCGCTGCGGATGCGATTAAGCGAACTGGAACAGCGTCTACGCGagc > 1:1034511/1‑68 (MQ=255) tCACCTGGCAGAGCAGGTTCAGCCGCTGCGGATGCGATTAAGCGAACTGGAACAGCGTCTACGCGagc > 1:742866/1‑68 (MQ=255) tCACCTGGCAGAGCAGGTTCAGCCGCTGCGGATGCGATTAAGCGAACTGGAACAGCGTCTACGCGagc > 1:1900350/1‑68 (MQ=255) aCCTGGCAGAGCAGGTTCAGCCGTTGCGGATGCGCTTAAGCGTACTGGAACAGCGTCTGCGTGagca > 1:2218468/1‑67 (MQ=255) aCCTGGCAGAGCAGGTTCAGCCGTTGCGGATGCGCTTAAGCGAACTGGAACAGCGTCTGCGTGagca > 1:961619/1‑67 (MQ=255) aCCTGGCAGAGCAGGTTCAGCCGTTGCGGATGCGCTTAAGCGAACTGGAACAGCGTCTGCGTGagca > 1:3400591/1‑67 (MQ=255) aCCTGGCAGAGCAGGTTCAGCCGTTGCGGATGCGCTTAAGCGAACTGGAACAGCGTCTGCGTGagc > 1:881425/1‑66 (MQ=255) ccTGGCAGAGCAGGTTCAGCCGTTGCGGATGCGCTTAAGCGAACTGGAACAGCGt < 1:2502463/55‑1 (MQ=255) cAGGTTCAGCCGCTGCGGATGCGATTAAGCGAACTGGAACAGCGTCTACGCGAGCAGCAAGAAGGTGAg > 1:2987077/1‑69 (MQ=255) cAGGTTCAGCCGCTGAGGATGCGATTAAGCGAACTGGAACAGCGTCTACGCGAGCAGCAAGAAGCTGAg > 1:3397770/1‑69 (MQ=255) ggTTCAGCCGCTGCGGATGCGATTAAGCGAACTGGAACAGCGTCTACGCGAGCAGCAAGAAGCTGAGCg > 1:2933701/1‑69 (MQ=255) ttCAGCCGCTGCGGATGCGATTAAGCGAACTGGAACAGCGTCTACGCGAGCAGCAAGAAGCTGAGCGTc < 1:1059388/69‑1 (MQ=255) ttCAGCCGCTGCGGATGCGATTAAGCGAACTGGAACAGCGTCTACGCGAGCAGCAAGAAGCTGAGCGTc < 1:2519368/69‑1 (MQ=255) ttCAGCCGCTGCGGATGCGATTAAGCGAACTGGAACAGCGTCTACGCGAGCAGCAAGAAGCTGAGCGTc < 1:2991857/69‑1 (MQ=255) tCAGCCGCTGCGGATGCGATTAAGCGAACTGGAACAGCGTCTACGCGAGCAGCAAGAAGCTGAGCGTct > 1:3418083/1‑69 (MQ=255) tCAGCCGCTGCGGATGCGATTAAGCGAACTGGAACAGCGTCTACGCGAGCAGCAAGAAGCTGAGCGTct > 1:1750044/1‑69 (MQ=255) tCAGCCGCTGCGGATGCGATTAAGCGAACTGGAACAGCGTCTACGCGAGCAGCAAGAAGCTGAGCGTct > 1:914845/1‑69 (MQ=255) cAGCCGTTGCGGATGCGCTTAAGCGAACTGGAACAGCGt < 1:2746563/39‑1 (MQ=37) cTGCGGATGCGATTAAGCGAACTGGAACAGCGTCTACGCGAGCAGCAAGAAGCTGAGCGTCTGCTGGCa < 1:875047/69‑1 (MQ=255) cTGCGGATGCGATTAAGCGAACTGGAACAGCGTCTACGCGAGCAGCAAGAAGCTGAGCGTCTGCTGGCa < 1:2357181/69‑1 (MQ=255) cTGCGGATGCGATTAAGCGAACTGGAACAGCGTCTACGCGAGCAGCAAGAAGCTGAGCGTCTGCTGGCa < 1:753567/69‑1 (MQ=255) cTGCGGATGCGATTAAGCGAACTGGAACAGCGTCTACGCGAGCAGCAAGAAGCTGAGCGTCTGCTGGCa < 1:1386724/69‑1 (MQ=255) cTGCGGATGCGATTAAGCGAACTGGAACAGCGTCTACGCGAGCAGCAAGAAGCTGAGCGTCTGCTGGCa < 1:3224818/69‑1 (MQ=255) cTGCGGATGCGATTAAGCGAACTGGAACAGCGTCTACGCGAGCAGCAAGAAGCTGAGCGTCTGCTGGCa < 1:2592138/69‑1 (MQ=255) cTGCGGATGCGATTAAGCGAACTGGAACAGCGTCTACGCGAGCAGCAAGAAGCTGAGCGTCTGCTGGCa < 1:2982265/69‑1 (MQ=255) gCGGATGCGATTAAGCGAACTGGAACAGCGTCTACGCGAGCAGCAAGAAGCTGAGCGTCTGCTGGCa < 1:2496993/67‑1 (MQ=255) | TTGCGCGAAGGGGTCGATCAGCGTCACCTGGCAGAGCAGGTTCAGCCGCTGCGGATGCGATTAAGCGAACTGGAACAGCGTCTACGCGAGCAGCAAGAAGCTGAGCGTCTGCTGGCA > minE/634528‑634644 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |