| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 68,539 | G→T | 51.4% | intergenic (‑91/‑569) | leuL ← / → leuO | leu operon leader peptide/DNA‑binding transcriptional activator |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 68,539 | 0 | G | T | 51.4% | ‑0.4 / 86.8 | 70 | intergenic (‑91/‑569) | leuL/leuO | leu operon leader peptide/DNA‑binding transcriptional activator |
| Reads supporting (aligned to +/- strand): ref base G (18/16); new base T (21/15); total (39/31) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 8.10e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.76e-01 | |||||||||||
ATGCTTTTAGAGTTACTGGATACAAAAACGGATGTCAACCCTGACGCAATAAAAACGTCCCGCCAGCGTGAGTTCTGCATCCGTAAAATTAGCTAATTGTGCTGCGGTGGTTAAAGTAAGCGA > minE/68474‑68596 | atGCTTTTAGAGTTACTGGATACAAAAACGGATGTCAACCCTGACGCAATAAAAACGTCCCGCCAg > 1:74277/1‑66 (MQ=255)atGCTTTTAGAGTTACTGGATACAAAAACGGATGTCAACCCTGACGCAATAAAAACGTCCCGCCAGc > 1:900105/1‑67 (MQ=255)atGCTTTTAGAGTTACTGGATACAAAAACGGATGTCAACCCTGACGCAATAAAAACGTCCCGCCAGc > 1:2621426/1‑67 (MQ=255)atGCTTTTAGAGTTACTGGATACAAAAACGGATGTCAACCCTGACGCAATAAAAACGTCCCGCCAGc > 1:3376985/1‑67 (MQ=255)atGCTTTTAGAGTTACTGGATACAAAAACGGATGTCAACCCTGACGCAATAAAAACGTCCCGCCAGc > 1:2986334/1‑67 (MQ=255) tGCTTTTAGAGTTACTGGATACAAAAACGGATGTCAACCCTGACGCAATAAAAACGTCCCGCCAGCGTg < 1:486815/69‑1 (MQ=255) gTTACTGGATACAAAAACGGATGTCAACCCTGACGCAATAAAAACGTCCCGCCAGCGTGAGTTCTGCAt > 1:801652/1‑69 (MQ=255) gTTACTGGATACAAAAACGGATGTCAACCCTGACGCAATAAAAACGTCCCGCCAGCGTGAGTTCTGCAt > 1:3342424/1‑69 (MQ=255) gTTACTGGATACAAAAACGGATGTCAACCCTGACGCAATAAAAACGTCCCGCCAGCGTGAGTTCTGCAt > 1:303947/1‑69 (MQ=255) ttACTGGATACAAAAACGGATGTCAACCCTGACGCAATAAAAACGTCCCGCCAGCGTGAGTTCTGCATc > 1:2655027/1‑69 (MQ=255) gATACAAAAACGGATGTCAACCCTGAAGCAATAAAAACGTCACGCCATTGTGAGTTCGGCATCCGTaaa > 1:2449580/1‑69 (MQ=255) gATACAAAAACGGATGTCAACCCTGAAGCAATAAAAACGTCACGCCATTGTGAGTTCGGCATCCGTaaa > 1:982330/1‑69 (MQ=255) gATACAAAAACGGATGTCAACCCTGAAGCAATAAAAACGTCACGCCATTGTGAGTTCGGCATCCGTaaa > 1:2461862/1‑69 (MQ=255) gATACAAAAACGGATGTCAACCCTGAAGCAATAAAAACGTCACGCCATTGTGAGTTCGGCATCCGTaaa > 1:2476925/1‑69 (MQ=255) gATACAAAAACGGATGTCAACCCTGAAGCAATAAAAACGTCACGCCATTGTGAGTTCGGCATCCGTaaa > 1:2557701/1‑69 (MQ=255) gATACAAAAACGGATGTCAACCCTGAAGCAATAAAAACGTCACGCCATTGTGAGTTCGGCATCCGTaaa > 1:2642834/1‑69 (MQ=255) gATACAAAAACGGATGTCAACCCTGAAGCAATAAAAACGTCACGCCATTGTGAGTTCGGCATCCGTaaa > 1:2842997/1‑69 (MQ=255) gATACAAAAACGGATGTCAACCCTGAAGCAATAAAAACGTCACGCCATTGTGAGTTCGGCATCCGTaaa > 1:3132536/1‑69 (MQ=255) gATACAAAAACGGATGTCAACCCTGAAGCAATAAAAACGTCACGCCATTGTGAGTTCGGCATCCGTaaa > 1:3132557/1‑69 (MQ=255) gATACAAAAACGGATGTCAACCCTGAAGCAATAAAAACGTCACGCCATTGTGAGTTCGGCATCCGTaaa > 1:36818/1‑69 (MQ=255) gATACAAAAACGGATGTCAACCCTGAAGCAATAAAAACGTCACGCCATTGTGAGTTCGGCATCCGTaaa > 1:497358/1‑69 (MQ=255) gATACAAAAACGGATGTCAACCCTGAAGCAATAAAAACGTCACGCCATTGTGAGTTCGGCATCCGTaaa > 1:63770/1‑69 (MQ=255) gATACAAAAACGGATGTCAACCCTGAAGCAATAAAAACGTCACGCCATTGTGAGTTCGGCATCCGTaaa > 1:800151/1‑69 (MQ=255) gATACAAAAACGGATGTCAACCCTGAAGCAATAAAAACGTCACGCCATTGTGAGTTCGGCATCCGTaaa > 1:868393/1‑69 (MQ=255) gATACAAAAACGGATGTCAACCCTGAAGCAATAAAAACGTCACGCCATTGTGAGTTCGGCATCCGTaaa > 1:1598495/1‑69 (MQ=255) gATACAAAAACGGATGTCAACCCTGAAGCAATAAAAACGTCACGCCATTGTGAGTTCGGCATCCGTaaa > 1:1721695/1‑69 (MQ=255) gATACAAAAACGGATGTCAACCCTGAAGCAATAAAAACGTCACGCCATTGTGAGTTCGGCATCCGTaaa > 1:1852527/1‑69 (MQ=255) gATACAAAAACGGATGTCAACCCTGAAGCAATAAAAACGTCACGCCATTGTGAGTTCGGCATCCGTaaa > 1:1884093/1‑69 (MQ=255) gATACAAAAACGGATGTCAACCCTGAAGCAATAAAAACGTCACGCCATTGTGAGTTCGGCATCCGTaa > 1:1080790/1‑68 (MQ=255) gATACAAAAACGGATGTCAACCCTGAAGCAATAAAAACGTCACGCCATTGCGAGTTCGGCATCCGTaaa > 1:1744236/1‑69 (MQ=255) gATACAAAAACGGATGTCAACCCTGAAGCAAGAAAAACGTCACGCCATTGTGAGTTCGGCATCCGTaaa > 1:1777870/1‑69 (MQ=255) aaaCGGATGTCAACCCTGACGCAATAAAAACGTCCCGCCAGCGTGAGTTCTGCATCCGTAAAATTAGCt < 1:187118/69‑1 (MQ=255) aaaCGGATGTCAACCCTGACGCAATAAAAACGTCCCGCCAGCGTGAGTTCTGCATCCGTAAAATTAGCt < 1:2607044/69‑1 (MQ=255) tCAACCCTGACGCAATAAAAACGTCCCGCCAGCGTGAGTTCTGCATCCGTAAAATTAGCTAATTGtgct > 1:71136/1‑69 (MQ=255) tCAACCCTGACGCAATAAAAACGTCCCGCCAGCGTGAGTTCTGCATCCGTAAAATTAGCTAATTGtgct > 1:1875003/1‑69 (MQ=255) gCAATAAAAACGTCCCGCCAGCGTGAGTTCTGCATCCGTAAAATTAGCTAATTGTGCTGCGGTGGTTaa > 1:1788881/1‑69 (MQ=255) gCAATAAAAACGTCACGCCATTGTGAGTTCGGCATCCGTAAAATTAGCTAATTGTGCTGCGGTGGtt < 1:1580840/67‑1 (MQ=255) gCAATAAAAACGTCACGCCATTGTGAGTTCGGCATCCGTAAAATTAGCTAATTGTGCTGCGGTGGtt < 1:1525317/67‑1 (MQ=255) gCAATAAAAACGTCACGCCATTGTGAGTTCGGCATCCGTAAAATTAGCTAATTGTGCTGCGGTGGtt < 1:828612/67‑1 (MQ=255) gCAATAAAAACGTCACGCCATTGTGAGTTCGGCATCCGTAAAATTAGCTAATTGTGCTGCGGTGGtt < 1:1338833/67‑1 (MQ=255) gCAATAAAAACGTCACGCCATTGTGAGTTCGGCATCCGTAAAATTAGCTAATTGTGCTGCGGTGGtt < 1:2232987/67‑1 (MQ=255) gCAATAAAAACGTCACGCCATTGTGAGTTCGGCATCCGTAAAATTAGCTAATTGTGCTGCGGTGGtt < 1:3341443/67‑1 (MQ=255) gCAATAAAAACGTCACGCCATTGTGAGTTCGGCATCCGTAAAATTAGCTAATTGTGCTGCGGTGGtt < 1:1135993/67‑1 (MQ=255) gCAATAAAAACGTCACGCCATTGTGAGTTCGGCATCCGTAAAATTAGCTAATTGTGCTGCGGTGGtt < 1:3051325/67‑1 (MQ=255) gCAATAAAAACGTCACGCCATTGTGAGTTCGGCATCCGTAAAATTAGCTAATTGTGCTGCGGTGGtt < 1:2568895/67‑1 (MQ=255) gCAATAAAAACGTCACGCCATTGTGAGTTCGGCATCCGTAAAATTAGCTAATTGTGCTGCGGTGGtt < 1:1670640/67‑1 (MQ=255) gCAATAAAAACGTCACGCCATTGTGAGTTCGGCATCCGTAAAATTAGCTAATTGTGCTGCGGTGGtt < 1:2952537/67‑1 (MQ=255) gCAATAAAAACGTCACGCCATTGTGAGTTCGGCATCCGTAAAATTAGCTAATTGTGCTGCGGTGGtt < 1:967850/67‑1 (MQ=255) gCAATAAAAACGTCACGCCATTGTGAGTTCGGCATCCGTAAAATTAGCTAATTGTGCTGCGGTGGtt < 1:230214/67‑1 (MQ=255) gCAATAAAAACGTCACGCCATTGTGAGTTCGGCATCCGTAAAATTAGCTAATTGTGCTGCGGTGGtt < 1:786668/67‑1 (MQ=255) gCAATAAAAACGTCACGCCATTGTGAGTTCGGCATCCGTAAAATTAGCTAATTGTGCTGCGGTGGtt < 1:2005176/67‑1 (MQ=255) cAATAAAAACGTCCCGCCAGCGTGAGTTCTGCATCCGTAAAAt > 1:3139941/1‑43 (MQ=255) cAATAAAAACGTCCCGCCAGCGTGAGTTCTGCATCCGTAAAATTAGCTAATTGTGCTGCGGTGGTTaa > 1:2138203/1‑68 (MQ=255) cAATAAAAACGTCCCGCCAGCGTGAGTTCTGCATCCGTAAAATTAGCTAATTGTGCTGCGGTGGTTaa > 1:631784/1‑68 (MQ=255) cAATAAAAACGTCCCGCCAGCGTGAGTTCTGCATCCGTAAAATTAGCTAATTGTGCTGCGGTGGTTaa > 1:2470028/1‑68 (MQ=255) tAAAAACGTCCCGCCAGCGTGAGTTCTGCATCCGTAAAATTAGCTAATTGTGCTGCGGTGGTTAAAGTa < 1:253723/69‑1 (MQ=255) aaaaaCGTCCCGCCAGCGTGAGTTCTGCATCCGTAAAATTAGCTAATTGTGCTGCGGTGGTTa > 1:819336/1‑63 (MQ=255) aaaaCGTCCCGCCAGCGTGAGTTCTGCATCCGTAAAATTAGCTAATTGTGCTGCGGTGGTTAAAGt < 1:201755/66‑1 (MQ=255) aaaaCGTCCCGCCAGCGTGAGTTCTGCATCCGTAAAATTAGCTAATTGTGCTGCGGTGGTTAAAGt < 1:2307890/66‑1 (MQ=255) aaaaCGTCCCGCCAGCGTGAGTTCTGCATCCGTAAAATTAGCTAATTGTGCTGCGGTGGTTAAAGt < 1:1921869/66‑1 (MQ=255) aaaaCGTCCCGCCAGCGTGAGTTCTGCATCCGTAAAATTAGCTAATTGTGCTGCGGTGGTTAAAGTaa > 1:601306/1‑68 (MQ=255) aaaaCGTCCCGCCAGCGTGAGTTCTGCATCCGTAAAATTAGCTAATTGTGCTGCGGTGGTTAAAGTaa > 1:2814519/1‑68 (MQ=255) aCGTCCCGCCAGCGTGAGTTCTGCATCCGTAAAATTAGCTAATTTTGCTGCGTTGGTTAAAGTAAGCGa < 1:2611997/69‑1 (MQ=255) aCGTCCCGCCAGCGTGAGTTCTGCATCCGTAAAATTAGCTAATTGTGCTGCGGTGGTTAAAGTAAGCGa < 1:2384372/69‑1 (MQ=255) aCGTCCCGCCAGCGTGAGTTCTGCATCCGTAAAATTAGCTAATTGTGCTGCGGTGGTTAAAGTAAGCGa < 1:524733/69‑1 (MQ=255) aCGTCCCGCCAGCGTGAGTTCTGCATCCGTAAAATTAGCTAATTGTGCTGCGGTGGTTAAAGTAAGCGa < 1:1672158/69‑1 (MQ=255) aCGTCCCGCCAGCGTGAGTTCTGCATCCGTAAAATTAGCTAATTGTGCTGCGGTGGTTAAAGTAAGCGa < 1:171691/69‑1 (MQ=255) aCGTCCCGCCAGCGTGAGTTCTGCATCCGTAAAATTAGCTAATTGTGCTGCGGTGGTTAAAGTAAGCGa < 1:451793/69‑1 (MQ=255) aCGTCCCGCCAGCGTGAGTTCTGCATCCGTAAAATTAGCTAATTGTGCTGCGGTGGTTAAAGTAAGCGa < 1:2368543/69‑1 (MQ=255) aCGTCCCGCCAGCGTGAGTTCTGCATCCGTAAAATTAGCTAATTGTGCTGCGGTGGTTAAAGTAAGCGa < 1:2853950/69‑1 (MQ=255) aCGTCCCGCCAGCGTGAGTTCTGCATCCGTAAAATTAGCTAATTGTGCTGCGGTGGTTAAAGTAAGCGa < 1:2792149/69‑1 (MQ=255) | ATGCTTTTAGAGTTACTGGATACAAAAACGGATGTCAACCCTGACGCAATAAAAACGTCCCGCCAGCGTGAGTTCTGCATCCGTAAAATTAGCTAATTGTGCTGCGGTGGTTAAAGTAAGCGA > minE/68474‑68596 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |