| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 636,129 | C→T | 35.8% | L1035L (CTC→CTT) | mukB → | fused chromosome partitioning proteins |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 636,129 | 0 | C | T | 35.8% | 63.9 / 53.2 | 92 | L1035L (CTC→CTT) | mukB | fused chromosome partitioning proteins |
| Reads supporting (aligned to +/- strand): ref base C (47/12); new base T (11/22); total (58/34) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.52e-05 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
GTCAGTACAACCAGGTGCTGGCTTCGCTGAAAAGTTCTTACGACACCAAAAAAGAGCTACTCAACGATCTGCAACGTGAATTGCAGGATATCGGCGTGCGTGCTGATAGCGGGGCAGAAGAGCGGGCGCG > minE/636068‑636197 | gTCAGTACAACCAGGTGCTGGCTTCGCTGAAAAGCTCTTACGACACCAAAAAAGAGCTACTCAACGAt < 1:1527094/68‑1 (MQ=255)gTCAGTACAACCAGGTGCTGGCTTCGCTGAAAAGCTCTTACGACACCAAAAAAGAGCTACTCAACGAt < 1:2218674/68‑1 (MQ=255) aCAACCAGGTGCTGGCTTCGCTGAAAAGTTCTTACGACACCAAAAAAGAGCTACTCAACGATCTGCAAc > 1:2551610/1‑69 (MQ=255) cAACCAGGTGCTGGCTTCGCTGAAAAGTTCTTACGACACCAAAAAAGAGCTACTCAACGATCTGCaa > 1:1318573/1‑67 (MQ=255) cAACCAGGTGCTGGCTTCGCTGAAAAGTTCTTACGACACCAAAAAAGAGCTACTCAACGATCTGCAAc > 1:50104/1‑68 (MQ=255) cAACCAGGTGCTGGCTTCGCTGAAAAGTTCTTACGACACCAAAAAAGAGCTACTCAACGATCTGCAAc > 1:1535514/1‑68 (MQ=255) cAACCAGGTGCTGGCTTCGCTGAAAAGTTCTTACGACACCAAAAAAGAGCTACTCAACGATCTGCAAc > 1:2013777/1‑68 (MQ=255) cAACCAGGTGCTGGCTTCGCTGAAAAGTTCTTACGACACCAAAAAAGAGCTACTCAACGATCTGCAAc > 1:2077272/1‑68 (MQ=255) cAACCAGGTGCTGGCTTCGCTGAAAAGTTCTTACGACACCAAAAAAGAGCTACTCAACGATCTGCAAc > 1:136581/1‑68 (MQ=255) cAACCAGGTGCTGGCTTCGCTGAAAAGTTCTTACGACACCAAAAAAGAGCTACTCAACGATCTGCAAc > 1:2542749/1‑68 (MQ=255) cAACCAGGTGCTGGCTTCGCTGAAAAGTTCTTACGACACCAAAAAAGAGCTACTCAACGATCTGCAAc > 1:301817/1‑68 (MQ=255) cAACCAGGTGCTGGCTTCGCTGAAAAGTTCTTACGACACCAAAAAAGAGCTACTCAACGATCTGCAAc > 1:3324272/1‑68 (MQ=255) cAACCAGGTGCTGGCTTCGCTGAAAAGTTCTTACGACACCAAAAAAGAGCTACTCAACGATCTGCAAc > 1:540733/1‑68 (MQ=255) gTGCTGGCTTCGCTGAAAAGTTCTTACGACACCAAAAAAGAGGTACTCAACGATCTGCAACGTGAAt > 1:408785/1‑67 (MQ=255) gTGCTGGCTTCGCTGAAAAGTTCTTACGACACCAAAAAAGAGCTACTCAACGATCTGCAACGTGAAt > 1:2862297/1‑67 (MQ=255) gTGCTGGCTTCGCTGAAAAGTTCTTACGACACCAAAAAAGAGCTACTCAACGATCTGCAACGTGAAt > 1:2838938/1‑67 (MQ=255) gTGCTGGCTTCGCTGAAAAGTTCTTACGACACCAAAAAAGAGCTACTCAACGATCTGCAACGTGAAt > 1:1036820/1‑67 (MQ=255) gTGCTGGCTTCGCTGAAAAGTTCTTACGACACCAAAAAAGAGCTACTCAACGATCTGCAACGTGAAt > 1:699264/1‑67 (MQ=255) gTGCTGGCTTCGCTGAAAAGTTCTTACGACACCAAAAAAGAGCTACTCAACGATCTGCAACGTGAAt > 1:3025441/1‑67 (MQ=255) gTGCTGGCTTCGCTGAAAAGTTCTTACGACACCAAAAAAGAGCTACTCAACGATCTGCAACGTGAAt > 1:743956/1‑67 (MQ=255) gTGCTGGCTTCGCTGAAAAGTTCTTACGACACCAAAAAAGAGCTACTCAACGATCTGCAACGTGAAt > 1:1777692/1‑67 (MQ=255) gTGCTGGCTTCGCTGAAAAGTTCTTACGACACCAAAAAAGAGCTACTCAACGATCTGCAACGTGAAt > 1:161815/1‑67 (MQ=255) gTGCTGGCTTCGCTGAAAAGTTCTTACGACACCAAAAAAGAGCTACTCAACGATCTGCAACGTGAAt > 1:1579557/1‑67 (MQ=255) gTGCTGGCTTCGCTGAAAAGTTCTTACGACACCAAAAAAGAGCTACTCAACGATCTGCAACGTGAAt > 1:800704/1‑67 (MQ=255) gTGCTGGCTTCGCTGAAAAGTTCTTACGACACCAAAAAAGAGCTACTCAACGATCTGCAACGTGAAt > 1:1501654/1‑67 (MQ=255) gCTGGCTTCGCTGAAAAGTTCTTACGACACCAAAAAAGAGCTACTCAACGATCTGCAACGTg > 1:898674/1‑62 (MQ=255) gCTGGCTTCGCTGAAAAGTTCTTACGACACCAAAAAAGAGCTACTCAACGATCTGCAACGTg > 1:2881893/1‑62 (MQ=255) gCTGGCTTCGCTGAAAAGTTCTTACGACACCAAAAAAGAGCTACTCAACGATCTGCAACGTg > 1:284986/1‑62 (MQ=255) gCTGGCTTCGCTGAAAAGTTCTTACGACACCAAAAAAGAGCTACTCAACGATCTGCAACGTg > 1:1315268/1‑62 (MQ=255) gCTGGCTTCGCTGAAAAGTTCTTACGACACCAAAAAAGAGCTACTCAACGATCTGCAACGTg > 1:3295507/1‑62 (MQ=255) gCTGGCTTCGCTGAAAAGTTCTTACGACACCAAAAAAGAGCTACTCAACGATCTGCAACGTg > 1:1627840/1‑62 (MQ=255) gCTGGCTTCGCTGAAAAGTTCTTACGACACCAAAAAAGAGCTACTCAACGATCTGCAACGTg > 1:361032/1‑62 (MQ=255) gCTGGCTTCGCTGAAAAGTTCTTACGACACCAAAAAAGAGCTACTCAACGATCTGCAACGTg > 1:2593319/1‑62 (MQ=255) gCTGGCTTCGCTGAAAAGTTCTTACGACACCAAAAAAGAGCTACTCAACGATCTGCAACGTg > 1:1334843/1‑62 (MQ=255) gCTGGCTTCGCTGAAAAGTTCTTACGACACCAAAAAAGAGCTACTCAACGATCTGCAACGTg > 1:1346331/1‑62 (MQ=255) gCTGGCTTCGCTGAAAAGTTCTTACGACACCAAAAAAGAGCTACTCAACGATCTGCAACGTg > 1:1440977/1‑62 (MQ=255) gCTGGCTTCGCTGAAAAGTTCTTACGACACCAAAAAAGAGCTACTCAACGATCTGCAACGTg > 1:1990609/1‑62 (MQ=255) gCTGGCTTCGCTGAAAAGTTCTTACGACACCAAAAAAGAGCTACTCAACGATCTGCAACGTGAAtag > 1:1399631/1‑65 (MQ=255) ggCTTCGCTGAAAAGTTCTTACGACACCAAAAAAGAGCTACTCAACGATCTGCAACGTGAATTGCAGGa < 1:459351/69‑1 (MQ=255) ggCTTCGCTGAAAAGTTCGTATGACACCAAAAAAGAGCTACTTAACGATCTGCAACGTGAATTGCAGGa < 1:1581685/69‑1 (MQ=255) ggCTTCGCTGAAAAGTTCGTATGACACCAAAAAAGAGCTACTTAACGATCTGCAACGTGAATTGCAGGa < 1:1593343/69‑1 (MQ=255) ggCTTCGCTGAAAAGTTCGTATGACACCAAAAAAGAGCTACTTAACGATCTGCAACGTGAATTGCAGGa < 1:3311121/69‑1 (MQ=255) ggCTTCGCTGAAAAGTTCGTATGACACCAAAAAAGAGCTACTTAACGATCTGCAACGTGAATTGCAGGa < 1:2978201/69‑1 (MQ=255) ggCTTCGCTGAAAAGTTCGTATGACACCAAAAAAGAGCTACTTAACGATCTGCAACGTGAATTGCAGGa < 1:120886/69‑1 (MQ=255) gCTTCGCTGAAAAGTTCGTATGACACCAAAAAAGAGCTACTTAACGATCTGCAACGTGAATTGCATGat > 1:1095005/1‑69 (MQ=255) gCTTCGCTGAAAAGTTCGTATGACACCAAAAAAGAGCTACTTAACGATCTGCAACGTGAATTGCAGGat > 1:1312211/1‑69 (MQ=255) gCTTCGCTGAAAAGTTCGTATGACACCAAAAAAGAGCTACTTAACGATCTGCAACGTGAATTGCAGGat > 1:1512032/1‑69 (MQ=255) gCTTCGCTGAAAAGTTCGTATGACACCAAAAAAGAGCTACTTAACGATCTGCAACGTGAATTGCAGGat > 1:2607440/1‑69 (MQ=255) gCTTCGCTGAAAAGTTCGTATGACACCAAAAAAGAGCTACTTAACGATCTGCAACGTGAATTGCAGGat > 1:839064/1‑69 (MQ=255) gCTTCGCTGAAAAGTTCGTATGACACCAAAAAAGAGCTACTTAACGATCTGCAACGTGAATTGCAGGat > 1:2984542/1‑69 (MQ=255) gCTTCGCTGAAAAGTTCGTATGACACCAAAAAAGAGCTACTTAACGATCTGCAACGTGAATTGCAGGat > 1:479009/1‑69 (MQ=255) gCTTCGCTGAAAAGTTCGTATGACACCAAAAAAGAGCTACTTAACGATCTGCAACGTGAATTGCAGGat > 1:52364/1‑69 (MQ=255) gCTTCGCTGAAAAGTTCGTATGACACCAAAAAAGAGCTACTTAACGATCTGCAACGTGAATTGCAGGat > 1:1294995/1‑69 (MQ=255) gCTTCGCTGAAAAGTTCGTATGACACCAAAAAAGAGCTACTTAACGATCTGCAACGTGAATTGCAGGat > 1:2011681/1‑69 (MQ=255) gCTTCGCTGAAAAGTTCGTATGACACCAAAAAAGAGCTACTTAACGATCTGCAACGTGAATTACAGGat > 1:1475444/1‑69 (MQ=255) gCTGAAAAGCTCTTACGACACCAAAAAAGAGCTACTCAACGAt < 1:882936/43‑1 (MQ=255) cTGAAAAGTTCTTACGACACCAAAAAAGAGCTACTCAACGATCTGCAACGTGAATTGCAGGATATCgg < 1:2957303/68‑1 (MQ=255) cTGAAAAGTTCTTACGACACCAAAAAAGAGCTACTCAACGATCTGCAACGTGAATTGCAGGATATCgg < 1:3023847/68‑1 (MQ=255) tctaaGTTCGTATGACACCAAAAAAGAGCTACTTAACGATCTGCAACGTGAATTGCATGATATCGgc < 1:1873267/64‑1 (MQ=255) ttCTTACGCCACCAAAAAAGAGCTACTCAACGATCTGCAACGTGAATTGCAGGATATCGGcgtgcgtg > 1:2171435/1‑68 (MQ=255) ttCTTACGACACCAAAAAAGAGCTACTCAACGATCTGCAACGTGAATTGCAGGATATCGGcgtgcgtg > 1:3173977/1‑68 (MQ=255) ttCTTACGACACCAAAAAAGAGCTACTCAACGATCTGCAACGTGAATTGCAGGATATCGGcgtgcgtg > 1:2983051/1‑68 (MQ=255) ttCTTACGACACCAAAAAAGAGCTACTCAACGATCTGCAACGTGAATTGCAGGATATCGGcgtgcgtg > 1:2908984/1‑68 (MQ=255) ttCTTACGACACCAAAAAAGAGCTACTCAACGATCTGCAACGTGAATTGCAGGATATCGGcgtgcgtg > 1:703296/1‑68 (MQ=255) ttCTTACGACACCAAAAAAGAGCTACTCAACGATCTGCAACGTGAATTGCAGGATATCGGcgtgcgtg > 1:1603783/1‑68 (MQ=255) ttCTTACGACACCAAAAAAGAGCTACTCAACGATCTGCAACGTGAATTGCAGGATATCGGcgtgcgtg > 1:2646841/1‑68 (MQ=255) ttCTTACGACACCAAAAAAGAGCTACTCAACGATCTGCAACGTGAATTGCAGGATATCGGcgtgcgtg > 1:2608053/1‑68 (MQ=255) ttCTTACGACACCAAAAAAGAGCTACTCAACGATCTGCAACGTGAATTGCAGGATATCGGcgtgcgtg > 1:2555252/1‑68 (MQ=255) ttCTTACGACACCAAAAAAGAGCTACTCAACGATCTGCAACGTGAATTGCAGGATATCGGcgtgcgtg > 1:679262/1‑68 (MQ=255) ttCGTATGACACCAAAAAAGAGCTACTTAACGATCTGCAACGTGAATTGCAGGATATCGgc < 1:1025855/61‑1 (MQ=255) ttCGTATGACACCAAAAAAGAGCTACTTAACGATCTGCAACGTGAATTGCAGGATATCGgc < 1:1041518/61‑1 (MQ=255) ttCGTATGACACCAAAAAAGAGCTACTTAACGATCTGCAACGTGAATTGCAGGATATCGgc < 1:2837595/61‑1 (MQ=255) ttCGTATGACACCAAAAAAGAGCTACTTAACGATCTGCAACGTGAATTGCAGGATATCGgc < 1:1181821/61‑1 (MQ=255) ttCGTATGACACCAAAAAAGAGCTACTTAACGATCTGCAACGTGAATTGCAGGATATCGgc < 1:3115649/61‑1 (MQ=255) ttCGTATGACACCAAAAAAGAGCTACTTAACGATCTGCAACGTGAATTGCAGGATATCGgc < 1:2347757/61‑1 (MQ=255) ttCGTATGACACCAAAAAAGAGCTACTTAACGATCTGCAACGTGAATTGCAGGATATCGgc < 1:1775682/61‑1 (MQ=255) ttCGTATGACACCAAAAAAGAGCTACTTAACGATCTGCAACGTGAATTGCAGGATATCGgc < 1:192172/61‑1 (MQ=255) ttCGTATGACACCAAAAAAGAGCTACTTAACGATCTGCAACGTGAATTGCAGGATATCGgc < 1:203745/61‑1 (MQ=255) ttCGTATGACACCAAAAAAGAGCTACTTAACGATCTGCAACGTGAATTGCAGGATATCGgc < 1:2107206/61‑1 (MQ=255) ttCGTATGACACCAAAAAAGAGCTACTTAACGATCTGCAACGTGAATTGCAGGATATCGgc < 1:2195483/61‑1 (MQ=255) ttCGTATGACACCAAAAAAGAGCTACTTAACGATCTGCAACGTGAATTGCAGGATATCGgc < 1:2407312/61‑1 (MQ=255) ttCGTATGACACCAAAAAAGAGCTACTTAACGATCTGCAACGTGAATTGCAGGATATCGgc < 1:2804967/61‑1 (MQ=255) ttCGTATGACACCAAAAAAGAGCTACTTAACGATCTGCAACGTGAATTGCAGGATATCGgc < 1:1281798/61‑1 (MQ=255) tCTTACGACACCAAAAAAGAGCTACTCAACGATCTGCAACGTGAATTGCAGGATATCGGCGTGCGTGCt < 1:2795506/69‑1 (MQ=255) tCTTACGACACCAAAAAAGAGCTACTCAACGATCTGCAACGTGAATTGCAGGATATCGGCGTGCGTGCt < 1:1637681/69‑1 (MQ=255) tCTTACGACACCAAAAAAGAGCTACTCAACGATCTGCAACGTGAATTGCAGGATATCGGCGTGCGTGCt < 1:1656660/69‑1 (MQ=255) tCTTACGACACCAAAAAAGAGCTACTCAACGATCTGCAACGTGAATTGCAGGATATCGGCGTGCGTGCt < 1:1521702/69‑1 (MQ=255) tCTTACGACACCAAAAAAGAGCTACTCAACGATCTGCAACGTGAATTGCAGGATATCGGCGTGCGTGCt < 1:3231450/69‑1 (MQ=255) tCTTACGACACCAAAAAAGAGCTACTCAACGATCTGCAACGTGAATTGCAGGATATCGGCGTGCGTGCt < 1:1900215/69‑1 (MQ=255) aaaaaaGAGCTACTCAACGATCTGCAACGTGAATTGCAGGATATCGGCGTGCGTGCTGATAGCGGGGc > 1:1259117/1‑68 (MQ=255) gCTACTTAACGATCTGCAACGTGAATTGCAGGATATCGgc < 1:1539031/40‑1 (MQ=255) cTACTTAACGATCTGCAACGTGAATTGCAGGATATCGgc < 1:1190665/39‑1 (MQ=255) cAACGATCTGCAACGTGAATTGCAGGATATCGGCGTGCGTGCTGATAGCGGGGCAGAAGAGCGGGcgcg < 1:1777376/69‑1 (MQ=255) cAACGATCTGCAACGTGAATTGCAGGATATCGGCGTGCGTGCTGATAGCGGGGCAGAAGAGCGGGcgcg < 1:717634/69‑1 (MQ=255) cAACGATCTGCAACGTGAATTGCAGGATATCGGCGTGCGTGCTGATAGCGGGGCAGAAGAGCGGGcgcg < 1:1380591/69‑1 (MQ=255) cAACGATCTGCAACGTGAATTGCAGGATATCGGCGTGCGTGCTGATAGCGGGGCAGAAGAGCGGGcgcg < 1:2990980/69‑1 (MQ=255) | GTCAGTACAACCAGGTGCTGGCTTCGCTGAAAAGTTCTTACGACACCAAAAAAGAGCTACTCAACGATCTGCAACGTGAATTGCAGGATATCGGCGTGCGTGCTGATAGCGGGGCAGAAGAGCGGGCGCG > minE/636068‑636197 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |