| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 636,861 | G→A | 37.9% | V1279V (GTG→GTA) | mukB → | fused chromosome partitioning proteins |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 636,861 | 0 | G | A | 37.9% | 26.4 / 28.6 | 50 | V1279V (GTG→GTA) | mukB | fused chromosome partitioning proteins |
| Reads supporting (aligned to +/- strand): ref base G (25/6); new base A (17/2); total (42/8) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.93e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.72e-01 | |||||||||||
CAACCAGGGGTTGCAGAACGTATCGTTTGGTCAGGTGAACAGCGTGCGTCTCAACGTGAACGTGCGTGAAACGCACGCCATGCTACTGGATGTGCTCTCTGAACAGCACGAGCAGCATC > minE/636798‑636916 | cAACCAGGGGTTGCAGAACGTATCGTTTGGTCAGGTGAACAGCGTGCGTCTCAACGTGAACGTGCGTGa < 1:65708/69‑1 (MQ=255)cAACCAGGGGTTGCAGAACGTATCGTTTGGTCAGGTGAACAGCGTGCGTCTCAACGTGAACGTGCGTGa < 1:178423/69‑1 (MQ=255) gtggTTGCAGAACGTATCGTTTGGCCAGGTGAACAGCGTACGTCTGAACGTGAACGTACGTGAAacgca < 1:2324041/67‑1 (MQ=255) ggggTTGCAGAACGTATCGTTTGGCCAGGTGAACAGCGTACGTCTGAACGTGAACGTACGTGAAacgca < 1:209567/69‑1 (MQ=255) tGCAGAACGTATCGTTTGGTCAGGTGAACAGCGTGCGTCTCAACGTGAACGTGCGTGAAACGCACGcc > 1:2376107/1‑68 (MQ=255) tGCAGAACGTATCGTTTGGTCAGGTGAACAGCGTGCGTCTCAACGTGAACGTGCGTGAAACGCACGcc > 1:714900/1‑68 (MQ=255) tGCAGAACGTATCGTTTGGTCAGGTGAACAGCGTGCGTCTCAACGTGAACGTGCGTGAAACGCACGCCa > 1:1000140/1‑69 (MQ=255) tGCAGAACGTATCGTTTGGTCAGGTGAACAGCGTGCGTCTCAACGTGAACGTGCGTGAAACGCACGCCa > 1:2602740/1‑69 (MQ=255) tGCAGAACGTATCGTTTGGTCAGGTGAACAGCGTGCGTCTCAACGTGAACGTGCGTGAAACGCACGCCa > 1:323119/1‑69 (MQ=255) tGCAGAACGTATCGTTTGGTCAGGTGAACAGCGTGCGTCTCAACGTGAACGTGCGTGAAACGCACGCCa > 1:1995542/1‑69 (MQ=255) tGCAGAACGTATCGTTTGGTCAGGTGAACAGCGTGCGTCTCAACGTGAACGTGCGTGAAACGCACGCCa > 1:1828048/1‑69 (MQ=255) tGCAGAACGTATCGTTTGGTCAGGTGAACAGCGTGCGTCTCAACGTGAACGTGCGTGAAACGCACGCCa > 1:1706891/1‑69 (MQ=255) tGCAGAACGTATCGTTTGGTCAGGTGAACAGCGTGCGTCTCAACGTGAACGTGCGTGAAACGCACGCCa > 1:1604302/1‑69 (MQ=255) gCAGAACGTATCGTTTGGTCAGGTGAACAGCGTGCGTCTCAACGTGAACGTGCGTGAAACGCACGCCAt < 1:3433839/69‑1 (MQ=255) gCAGAACGTATCGTTTGGTCAGGTGAACAGCGTGCGTCTCAACGTGAACGTGCGTGAAACGCACGCCAt < 1:3373915/69‑1 (MQ=255) cAGAACGTATCGTTTGGTCAGGTGAACAGCGTGCGTCTCAACGTGAACGTGCGTGa < 1:491180/56‑1 (MQ=255) aaCGTATCGTTTGGCCAGGTGAACAGCGTACGTCTGAACGTGAACGTACGTGAAACGCACGTCATGCtg > 1:665822/1‑68 (MQ=255) aaCGTATCGTTTGGCCAGGTGAACAGCGTACGTCTGAACGTGAACGTACGTGAAACGCACGCCATGCtg > 1:30678/1‑68 (MQ=255) aaCGTATCGTTTGGCCAGGTGAACAGCGTACGTCTGAACGTGAACGTACGTGAAACGCACGCCATGCtg > 1:444230/1‑68 (MQ=255) aaCGTATCGTTTGGCCAGGTGAACAGCGTACGTCTGAACGTGAACGTACGTGAAACGCACGCCATGCtg > 1:3327198/1‑68 (MQ=255) aaCGTATCGTTTGGCCAGGTGAACAGCGTACGTCTGAACGTGAACGTACGTGAAACGCACGCCATGCtg > 1:3344189/1‑68 (MQ=255) aaCGTATCGTTTGGCCAGGTGAACAGCGTACGTCTGAACGTGAACGTACGTGAAACGCACGCCATGCtg > 1:3386944/1‑68 (MQ=255) aaCGTATCGTTTGGCCAGGTGAACAGCGTACGTCTGAACGTGAACGTACGTGAAACGCACGCCATGCtg > 1:374859/1‑68 (MQ=255) aaCGTATCGTTTGGCCAGGTGAACAGCGTACGTCTGAACGTGAACGTACGTGAAACGCACGCCATGCtg > 1:2840384/1‑68 (MQ=255) aaCGTATCGTTTGGCCAGGTGAACAGCGTACGTCTGAACGTGAACGTACGTGAAACGCACGCCATGCtg > 1:921127/1‑68 (MQ=255) aaCGTATCGTTTGGCCAGGTGAACAGCGTACGTCTGAACGTGAACGTACGTGAAACGCACGCCATGCtg > 1:971496/1‑68 (MQ=255) aaCGTATCGTTTGGCCAGGTGAACAGCGTACGTCTGAACGTGAACGTACGTGAAACGCACGCCATGCtg > 1:2753598/1‑68 (MQ=255) aaCGTATCGTTTGGCCAGGTGAACAGCGTACGTCTGAACGTGAACGTACGTGAAACGCACGCCATGCtg > 1:1429371/1‑68 (MQ=255) aaCGTATCGTTTGGCCAGGTGAACAGCGTACGTCTGAACGTGAACGTACGTGAAACGCACGCCATGCtg > 1:152387/1‑68 (MQ=255) aaCGTATCGTTTGGCCAGGTGAACAGCGTACGTCTGAACGTGAACGTACGTGAAACGCACGCCATGCtg > 1:2536804/1‑68 (MQ=255) aaCGTATCGTTTGGCCAGGTGAACAGCGTACGTCTGAACGTGAACGTACGTGAAACGCACGCCATGCtg > 1:2280454/1‑68 (MQ=255) aaCGTATCGTTTGGCCAGGTGAACAGCGTACGTCTGAACGTGAACGTACGTGAAACGCACGCCATGCtg > 1:28173/1‑68 (MQ=255) aaCGTATCGTTTGGCCAGGTGAACAGCGTACGTCTGAACGTGAACGTACGTGAAACGCACGCCATGCt > 1:2598300/1‑68 (MQ=255) gTATCGTTTGGTCAGGTGAACAGCGTGCGTCTCAACGTGAACGTGCGTGAAACGCACGCCATGCTACTg < 1:2710583/69‑1 (MQ=255) tCGTTTGGTCAGGTGAACAGCGTGCGTCTCAACTTGAACGTGCGTGAAACGCACGCCATGCTACTGGAt > 1:2034366/1‑69 (MQ=255) tCGTTTGGTCAGGTGAACAGCGTGCGTCTCAACGTGAACGTGCGTGAAACGCACGCCATGCTACTGGAt > 1:2532771/1‑69 (MQ=255) tCGTTTGGTCAGGTGAACAGCGTGCGTCTCAACGTGAACGTGCGTGAAACGCACGCCATGCTACTGGAt > 1:771969/1‑69 (MQ=255) tCGTTTGGTCAGGTGAACAGCGTGCGTCTCAACGTGAACGTGCGTGAAACGCACGCCATGCTACTGGAt > 1:1731215/1‑69 (MQ=255) tCGTTTGGTCAGGTGAACAGCGTGCGTCTCAACGTGAACGTGCGTGAAACGCACGCCATGCTACTGGAt > 1:507706/1‑69 (MQ=255) tCGTTTGGTCAGGTGAACAGCGTGCGTCTCAACGTGAACGTGCGTGAAACGCACGCCATGCTACTGGAt > 1:2178640/1‑69 (MQ=255) tCGTTTGGTCAGGTGAACAGCGTGCGTCTCAACGTGAACGTGCGTGAAACGCACGCCATGCTACTGGAt > 1:400830/1‑69 (MQ=255) tCGTTTGGTCAGGTGAACAGCGTGCGTCTCAACGTGAACGTGCGTGAAACGCACGCCATGCTACTGGAt > 1:3427327/1‑69 (MQ=255) tCGTTTGGTCAGGTGAACAGCGTGCGTCTCAACGTGAACGTGCGTGAAACGCACGCCATGCTACTGGAt > 1:3287140/1‑69 (MQ=255) tCGTTTGGTCAGGTGAACAGCGTGCGTCTCAACGTGAACGTGCGTGAAACGCACGCCATGCTACTGGAt > 1:2701611/1‑69 (MQ=255) tCGTTTGGTCAGGTGAACAGCGTGCGTCTCAACGTGAACGTGCGTGAAACGCACGCCATGCTACTGGAt > 1:2912774/1‑69 (MQ=255) gAACAGCGTGCGTCTCAACGTGAACGTGCGTGAAACGCACGCCATGCTACTGGATGTGCTCTCTGAAc > 1:2566288/1‑68 (MQ=255) gAACAGCGTGCGTCTCAACGTGAACGTGCGTGAAACGCACGCCATGCTACTGGATGTGCTCTCTGAAc > 1:325110/1‑68 (MQ=255) gAACAGCGTGCGTCTCAACGTGAACGTGCGTGAAACGCACGCCATGCTACTGGATGTGCTCTCTGAAc > 1:1696169/1‑68 (MQ=255) gAACAGCGTGCGTCTCAACGTGAACGTGCGTGAAACGCACGCCATGCTACTGGATGTGCTCTCTGAAc > 1:3101440/1‑68 (MQ=255) gAACAGCGTGCGTCTCAACGTGAACGTGCGTGAAACGCACGCCATGCTACTGGATGTGCTCTCTGAAc > 1:1013241/1‑68 (MQ=255) aacgtgCGTGAAACGCACGCCATGCTACTGGATGTGCTCTCTGAACAGCACGAGCAGCATc < 1:257037/61‑1 (MQ=255) aacgtgCGTGAAACGCACGCCATGCTACTGGATGTGCTCTCTGAACAGCACGAGCAGCATc < 1:497089/61‑1 (MQ=255) aacgtgCGTGAAACGCACGCCATGCTACTGGATGTGCTCTCTGAACAGCACGAGCAGCATc < 1:3231350/61‑1 (MQ=255) aacgtgCGTGAAACGCACGCCATGCTACTGGATGTGCTCTCTGAACAGCACGAGCAGCATc < 1:774749/61‑1 (MQ=255) | CAACCAGGGGTTGCAGAACGTATCGTTTGGTCAGGTGAACAGCGTGCGTCTCAACGTGAACGTGCGTGAAACGCACGCCATGCTACTGGATGTGCTCTCTGAACAGCACGAGCAGCATC > minE/636798‑636916 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |