| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 643,235 | G→A | 22.6% | D149D (GAC→GAT) | ompF ← | outer membrane porin 1a (Ia;b;F) |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 643,235 | 0 | G | A | 22.6% | 158.8 / 34.6 | 114 | D149D (GAC→GAT) | ompF | outer membrane porin 1a (Ia;b;F) |
| Reads supporting (aligned to +/- strand): ref base G (24/64); new base A (18/8); total (42/72) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.66e-04 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.38e-01 | |||||||||||
Only 100 of 124 total aligned reads displayed.
ATCAACCAGACCAAAGAAGTTGGAGTTACGATAGGTAGCAACGCCGCCAACACGACCAACGAAGAAGTCATCGCTGTATGCAGTATCACCACCAAATTCTGGCAGCATATCGGTGTAACCCAGTGCATCATAAAC > minE/643169‑643303 | aTCAACCAGACCAAAGATGTTGGAGTTACGATAGGTAGCAACGCCGCCAACACGACCAACGAAGAAGTc < 1:1515352/69‑1 (MQ=255)aTCAACCAGACCAAAGAAGTTGGAGTTACGATAGGTAGCAACGCCGCCAACACGACCAACGAAGAAGTc < 1:957303/69‑1 (MQ=255)aTCAACCAGACCAAAGAAGTTGGAGTTACGATAGGTAGCAACGCCGCCAACACGACCAACGAAGAAGTc < 1:1767242/69‑1 (MQ=255)aTCAACCAGACCAAAGAAGTTGGAGTTACGATAGGTAGCAACGCCGCCAACACGACCAACGAAGAAGTc < 1:3139302/69‑1 (MQ=255)aTCAACCAGACCAAAGAAGTTGGAGTTACGATAGGTAGCAACGCCGCCAACACGACCAACGAAGAAGTc < 1:3140775/69‑1 (MQ=255)aTCAACCAGACCAAAGAAGTTGGAGTTACGATAGGTAGCAACGCCGCCAACACGACCAACGAAGAAGTc < 1:1620329/69‑1 (MQ=255)aTCAACCAGACCAAAGAAGTTGGAGTTACGATAGGTAGCAACGCCGCCAACACGACCAACGAAGAAGTc < 1:1907168/69‑1 (MQ=255)aTCAACCAGACCAAAGAAGTTGGAGTTACGATAGGTAGCAACGCCGCCAACACGACCAACGAAGAAGTc < 1:3162554/69‑1 (MQ=255)aTCAACCAGACCAAAGAAGTTGGAGTTACGATAGGTAGCAACGCCGCCAACACGACCAACGAAGAAGTc < 1:1458551/69‑1 (MQ=255)aTCAACCAGACCAAAGAAGTTGGAGTTACGATAGGTAGCAACGCCGCCAACACGACCAACGAAGAAGTc < 1:3225458/69‑1 (MQ=255)aTCAACCAGACCAAAGAAGTTGGAGTTACGATAGGTAGCAACGCCGCCAACACGACCAACGAAGAAGTc < 1:3229467/69‑1 (MQ=255)aTCAACCAGACCAAAGAAGTTGGAGTTACGATAGGTAGCAACGCCGCCAACACGACCAACGAAGAAGTc < 1:2240601/69‑1 (MQ=255) ccaCCAGACCAAAGAAGTTGGAGTTACGATAGGTAGCAACGCCGCCAACACGACCAACGAAGAAGTCAt < 1:3372394/67‑1 (MQ=255) cAACCAGACCAAAGAAGTTGGAGTTACGATAGGTAGCAACGCCGCCAACACGACCAACGAAGAAGTCAt < 1:1765231/69‑1 (MQ=255) cAACCAGACCAAAGAAGTTGGAGTTACGATAGGTAGCAACGCCGCCAACACGACCAACGAAGAAGTCAt < 1:1264841/69‑1 (MQ=255) ccAGACCAAAGAAGTTGGAGTTACGATAGGTAGCAACGCCGCCAACACGACCAACGAAGAAATCATCGc < 1:1505310/69‑1 (MQ=255) ccAGACCAAAGAAGTTGGAGTTACGATAGGTAGCAACGCCGCCAACACGACCAACGAAGAAATCATCGc < 1:658382/69‑1 (MQ=255) ccAGACCAAAGAAGTTGGAGTTACGATAGGTAGCAACGCCGCCAACACGACCAACGAAGAAATCATCGc < 1:1703154/69‑1 (MQ=255) ccAGACCAAAGAAGTTGGAGTTACGATAGGTAGCAACGCCGCCAACACGACCAACGAAGAAATCATCGc < 1:1340104/69‑1 (MQ=255) cAGACCAAAGAAGTTGGAGTTACGATAGGTAGCAACGCCGCCAACACGACCAACGAAGAAGTCATCGc > 1:2180665/1‑68 (MQ=255) cAGACCAAAGAAGTTGGAGTTACGATAGGTAGCAACGCCGCCAACACGACCAACGAAGAAGTCATCGc > 1:1796134/1‑68 (MQ=255) cAGACCAAAGAAGTTGGAGTTACGATAGGTAGCAACGCCGCCAACACGACCAACGAAGAAATCATCGc < 1:2058103/68‑1 (MQ=255) cAGACCAAAGAAGTTGGAGTTACGATAGGTAGCAACGCCGCCAACACGACCAACGAAGAAATCATCGc < 1:3413845/68‑1 (MQ=255) aGACCAAAGAAGTTGGAGTTACGATAGGTAGCAACGCCGCCAACACGACCAACGAAGAAGTCATCGCTg < 1:3302409/69‑1 (MQ=255) agaagTTGGAGTTACGATAGGTAGCAACGCCGCCAACACGACCAACGAAGAAGTCATCGCTGTATGCAg < 1:3196370/69‑1 (MQ=255) agaagTTGGAGTTACGATAGGTAGCAACGCCGCCAACACGACCAACGAAGAAGTCATCGCTGTATGCAg < 1:1719640/69‑1 (MQ=255) agaagTTGGAGTTACGATAGGTAGCAACGCCGCCAACACGACCAACGAAGAAGTCATCGCTGTATGCAg < 1:384384/69‑1 (MQ=255) agaagTTGGAGTTACGATAGGTAGCAACGCCGCCAACACGACCAACGAAGAAGTCATCGCTGTATGCAg < 1:805092/69‑1 (MQ=255) agaagTTGGAGTTACGATAGGTAGCAACGCCGCCAACACGACCAACGAAGAAGTCATCGCTGTATGCAg < 1:1583784/69‑1 (MQ=255) agaagTTGGAGTTACGATAGGTAGCAACGCCGCCAACACGACCAACGAAGAAGTCATCGCTGTATGCAg < 1:1467254/69‑1 (MQ=255) gaagTTGGAGTTACGATAGGTAGCAACGCCGCCAACACGACCAACGAAGAAGTCATCGCTGTATGCAg < 1:282003/68‑1 (MQ=255) gaagTTGGAGTTACGATAGGTAGCAACGCCGCCAACACGACCAACGAAGAAGTCATCGCTGTATGCAg < 1:353210/68‑1 (MQ=255) aagTTGGAGTTACGATAGGTAGCAACGCCGCCAACACGACCAACGAAGAAGTCATc < 1:44512/56‑1 (MQ=255) aagTTGGAGTTACGATAGGTAGCAACGCCGCCAACACGACCAACGAAGAAGTCATc < 1:3379449/56‑1 (MQ=255) aagTTGGAGTTACGATAGGTAGCAACGCCGCCAACACGACCAACGAAGAAGTCATc < 1:18991/56‑1 (MQ=255) aagTTGGAGTTACGATAGGTAGCAACGCCGCCAACACGACCAACGAAGAAGTCATc < 1:1591268/56‑1 (MQ=255) aagTTGGAGTTACGATAGGTAGCAACGCCGCCAACACGACCAACGAAGAAGTCATc < 1:2795650/56‑1 (MQ=255) aagTTGGAGTTACGATAGGTAGCAACGCCGCCAACACGACCAACGAAGAAGTCATc < 1:799334/56‑1 (MQ=255) aagTTGGAGTTACGATAGGTAGCAACGCCGCCAACACGACCAACGAAGAAGTCATc < 1:1041511/56‑1 (MQ=255) aagTTGGAGTTACGATAGGTAGCAACGCCGCCAACACGACCAACGAAGAAGTCATc < 1:3111775/56‑1 (MQ=255) aagTTGGAGTTACGATAGGTAGCAACGCCGCCAACACGACCAACGAAGAAGTCATc < 1:2322069/56‑1 (MQ=255) agTTGGAGTTACGATAGGTAGCAACGCCGCCAACACGACCAACGAAGAAGTCAt > 1:872173/1‑54 (MQ=255) agTTGGAGTTACGATAGGTAGCAACGCCGCCAACACGACCAACGAAGAAGTCATCGCTGTATGCAGTAt > 1:2250611/1‑69 (MQ=255) agTTGGAGTTACGATAGGTAGCAACGCCGCCAACACGACCAACGAAGAAGTCATCGCTGTATGCAGTAt > 1:908709/1‑69 (MQ=255) agTTGGAGTTACGATAGGTAGCAACGCCGCCAACACGACCAACGAAGAAGTCATCGCTGTATGCAGTAt > 1:2696451/1‑69 (MQ=255) agTTGGAGTTACGATAGGTAGCAACGCCGCCAACACGACCAACGAAGAAGTCATCGCTGTATGCAGTAt > 1:117640/1‑69 (MQ=255) agTTGGAGTTACGATAGGTAGCAACGCCGCCAACACGACCAACGAAGAAGTCATCGCTGTATGCAGTAt > 1:2563540/1‑69 (MQ=255) agTTGGAGTTACGATAGGTAGCAACGCCGCCAACACGACCAACGAAGAAGTCATCGCTGTATGCAGTAt > 1:1401063/1‑69 (MQ=255) agTTGGAGTTACGATAGGTAGCAACGCCGCCAACACGACCAACGAAGAAGTCATCGCTGTATGCAGTAt > 1:1404393/1‑69 (MQ=255) agTTGGAGTTACGATAGGTAGCAACGCCGCCAACACGACCAACGAAGAAGTCATCGCTGTATGCAGTAt > 1:1428926/1‑69 (MQ=255) agTTGGAGTTACGATAGGTAGCAACGCCGCCAACACGACCAACGAAGAAGTCATCGCTGTATGCAGTAt > 1:607968/1‑69 (MQ=255) agTTGGAGTTACGATAGGTAGCAACGCCGCCAACACGACCAACGAAGAAGTCATCGCTGTATGCAGTAt > 1:55121/1‑69 (MQ=255) agTTGGAGTTACGATAGGTAGCAACGCCGCCAACACGACCAACGAAGAAGTCATCGCTGTATGCAGTAt > 1:532162/1‑69 (MQ=255) agTTGGAGTTACGATAGGTAGCAACGCCGCCAACACGACCAACGAAGAAGTCATCGCTGTATGCAGTAt > 1:461820/1‑69 (MQ=255) agTTGGAGTTACGATAGGTAGCAACGCCGCCAACACGACCAACGAAGAAGTCATCGCTGTATGCAGTAt > 1:1948494/1‑69 (MQ=255) agTTGGAGTTACGATAGGTAGCAACGCCGCCAACACGACCAACGAAGAAGTCATCGCTGTATGCAGTAt > 1:2960976/1‑69 (MQ=255) gTAGCAACGCCGCCAACACGACCAACGAAGAAGTCATCGCTGTATGCAGTATCACCACCAAATTCTGgc > 1:2948228/1‑69 (MQ=255) gTAGCAACGCCGCCAACACGACCAACGAAGAAGTCATCGCTGTATGCAGTATCACCACCAAATTCTGgc > 1:1377944/1‑69 (MQ=255) gTAGCAACGCCGCCAACACGACCAACGAAGAAGTCATCGCTGTATGCAGTATCAACACCAAATTCTGgc > 1:1681201/1‑69 (MQ=255) aaCGCCGCCAACACGACCAACGAAGAAGTCATCGCTGTATGCAGTATCACCACCAAATTCTGgcagca < 1:2625664/68‑1 (MQ=255) aaCGCCGCCAACACGACCAACGAAGAAGTCATCGCTGTATGCAGTATCACCACCAAATTCTGgcagca < 1:1180211/68‑1 (MQ=255) gccgccAACACGACCAACGAAGAAGTCATCGCTGTATGCAGTATCACCACCAAATTCTGGCAGCATATc < 1:10462/69‑1 (MQ=255) gccgccAACACGACCAACGAAGAAGTCATCGCTGTATGCAGTATCACCACCAAATTCTGGCAGCATATc < 1:3350152/69‑1 (MQ=255) gccgccAACACGACCAACGAAGAAGTCATCGCTGTATGCAGTATCACCACCAAATTCTGGCAGCATATc < 1:1584962/69‑1 (MQ=255) gccgccAACACGACCAACGAAGAAGTCATCGCTGTATGCAGTATCACCACCAAATTCTGGCAGCATATc < 1:2349572/69‑1 (MQ=255) gccgccAACACGACCAACGAAGAAGTCATCGCTGTATGCAGTATCACCACCAAATTCTGGCAGCATATc < 1:1574455/69‑1 (MQ=255) gccgccAACACGACCAACGAAGAAGTCATCGCTGTATGCAGTATCACCACCAAATTCTGGCAGCATATc < 1:263293/69‑1 (MQ=255) gccgccAACACGACCAACGAAGAAGTCATCGCTGTATGCAGTATCACCACCAAATTCTGGCAGCATATc < 1:754084/69‑1 (MQ=255) gccgccAACACGACCAACGAAGAAGTCATCGCTGTATGCAGTATCACCACCAAATTCTGGCAGCATATc < 1:792235/69‑1 (MQ=255) gccgccAACACGACCAACGAAGAAGTCATCGCTGTATGCAGTATCACCACCAAATTCTGGCAGCATATc < 1:3039462/69‑1 (MQ=255) gccgccAACACGACCAACGAAGAAGTCATCGCTGTATGCAGTATCACCACCAAATTCTGGCAGCATATc < 1:1235895/69‑1 (MQ=255) gccgccAACACGACCAACGAAGAAATCATCGCTGTATGCAGTAt > 1:688714/1‑44 (MQ=255) gccgccAACACGACCAACGAAGAAATCATCGCTGTATGCAGTATCACCACCAAATTCTGGCAGCatat > 1:3062957/1‑68 (MQ=255) gccgccAACACGACCAACGAAGAAATCATCGCTGTATGCAGTATCACCACCAAATTCTGGCAGCATATc > 1:1366753/1‑69 (MQ=255) gccgccAACACGACCAACGAAGAAATCATCGCTGTATGCAGTATCACCACCAAATTCTGGCAGCATATc > 1:1537152/1‑69 (MQ=255) gccgccAACACGACCAACGAAGAAATCATCGCTGTATGCAGTATCACCACCAAATTCTGGCAGCATATc > 1:1277152/1‑69 (MQ=255) gccgccAACACGACCAACGAAGAAATCATCGCTGTATGCAGTATCACCACCAAATTCTGGCAGCATATc > 1:356647/1‑69 (MQ=255) gccgccAACACGACCAACGAAGAAATCATCGCTGTATGCAGTATCACCACCAAATTCTGGCAGCATATc > 1:184460/1‑69 (MQ=255) gccgccAACACGACCAACGAAGAAATCATCGCTGTATGCAGTATCACCACCAAATTCTGGCAGCATATc > 1:2039228/1‑69 (MQ=255) gccgccAACACGACCAACGAAGAAATCATCGCTGTATGCAGTATCACCACCAAATTCTGGCAGCATATc > 1:3253989/1‑69 (MQ=255) gccgccAACACGACCAACGAAGAAATCATCGCTGTATGCAGTATCACCACCAAATTCTGGCAGCATATc > 1:3205382/1‑69 (MQ=255) gccgccAACACGACCAACGAAGAAATCATCGCTGTATGCAGTATCACCACCAAATTCTGGCAGCATATc > 1:269146/1‑69 (MQ=255) gccgccAACACGACCAACGAAGAAATCATCGCTATATGCAGTATCACCACCAAATTCTGGCAGCATATc > 1:1754285/1‑69 (MQ=255) cAACGAAGAAGTCATCGCTGTATGCAGTATCACCACCAAATTCTGGCAGCATATCGGTGTAACCCAGTg < 1:2965326/69‑1 (MQ=255) cAACGAAGAAGTCATCGCTGTATGCAGTATCACCACCAAATTCTGGCAGCATATCGGTGTAACCCAGTg < 1:3042991/69‑1 (MQ=255) aCGAAGAAGTCATCGCTGTATGCAGTATCACCACCAAATTCTGGCAGCATATCGGTGTAACCCAGTGca < 1:2588326/69‑1 (MQ=255) aCGAAGAAGTCATCGCTGTATGCAGTATCACCACCAAATTCTGGCAGCATATCGGTGTAACCCAGTGca < 1:2908484/69‑1 (MQ=255) aCGAAGAAGTCATCGCTGTATGCAGTATCACCACCAAATTCTGGCAGCATATCGGTGTAACCCAGTGca < 1:933465/69‑1 (MQ=255) aCGAAGAAGTCATCGCTGTATGCAGTATCACCACCAAATTCTGGCAGCATATCGGTGTAACCCAGTGca < 1:1074880/69‑1 (MQ=255) aCGAAGAAGTCATCGCTGTATGCAGTATCACCACCAAATTCTGGCAGCATATCGGTGTAACCCAGTGca < 1:2845461/69‑1 (MQ=255) aCGAAGAAGTCATCGCTGTATGCAGTATCACCACCAAATTCTGGCAGCATATCGGTGTAACCCAGTGca < 1:1636460/69‑1 (MQ=255) aCGAAGAAGTCATCGCTGTATGCAGTATCACCACCAAATTCTGGCAGCATATCGGTGTAACCCAGTGca < 1:1675607/69‑1 (MQ=255) aCGAAGAAGTCATCGCTGTATGCAGTATCACCACCAAATTCTGGCAGCATATCGGTGTAACCCAGTGca < 1:2936251/69‑1 (MQ=255) aCGAAGAAGTCATCGCTGTATGCAGTATCACCACCAAATTCTGGCAGCATATCGGTGTAACCCAGTGca < 1:188649/69‑1 (MQ=255) gTCATCGCTGTATGCAGTATCACCACCAAATTCTGGCAGCATATCGGTGTAACCCAGTGCATCATAAAc < 1:2662793/69‑1 (MQ=255) gTCATCGCTGTATGCAGTATCACCACCAAATTCTGGCAGCATATCGGTGTAACCCAGTGCATCATAAAc < 1:1989608/69‑1 (MQ=255) gTCATCGCTGTATGCAGTATCACCACCAAATTCTGGCAGCATATCGGTGTAACCCAGTGCATCATAAAc < 1:780268/69‑1 (MQ=255) gTCATCGCTGTATGCAGTATCACCACCAAATTCTGGCAGCATATCGGTGTAACCCAGTGCATCATAAAc < 1:2396980/69‑1 (MQ=255) gTCATCGCTGTATGCAGTATCACCACCAAATTCTGGCAGCATATCGGTGTAACCCAGTGCATCATAAAc < 1:3013060/69‑1 (MQ=255) gTCATCGCTGTATGCAGTATCACCACCAAATTCTGGCAGCATATCGGTGTAACCCAGTGCATCACAAAc < 1:1441523/69‑1 (MQ=255) | ATCAACCAGACCAAAGAAGTTGGAGTTACGATAGGTAGCAACGCCGCCAACACGACCAACGAAGAAGTCATCGCTGTATGCAGTATCACCACCAAATTCTGGCAGCATATCGGTGTAACCCAGTGCATCATAAAC > minE/643169‑643303 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |