| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 646,579 | C→T | 100% | A159A (GCG→GCA) | pncB ← | nicotinate phosphoribosyltransferase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 646,579 | 0 | C | T | 85.4% | 41.5 / 0.1 | 27 | A159A (GCG→GCA) | pncB | nicotinate phosphoribosyltransferase |
| Reads supporting (aligned to +/- strand): ref base C (1/3); new base T (0/23); total (1/26) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.48e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.87e-01 | |||||||||||
| Rejected as polymorphism: E-value score below prediction cutoff. | |||||||||||
| Rejected as polymorphism: Variant not supported by required number of reads on each strand. | |||||||||||
GGGTGCCAAAATCCATCAGATGGAAGCGCGACATATCAAGACCGGCGGTTAACGCCGAGAAGTCGACTAATTTGCTTTCCAGCGTGTCGAGGGCTTGCGCAACGTCGGCCTGCGGTG > minE/646527‑646643 | gggTGCCAAAATCCATCAGATGGAAGCGCGACATATCAAGACCGGCGGTTAACGCCGAGAAGTCGACTa < 1:2132305/69‑1 (MQ=255)gggTGCCAAAATCCATCAGATGGAAGCGCGACATATCAAGACCGGCGGTTAACGCCGAGAAGTCGACTa < 1:1030193/69‑1 (MQ=255) aCCGGCGGTTACCGCCGAGAAGTCGACTAATTTGCTTTCCAGCGTGTCGAGGGCTTGCGCAACGTCGGc > 1:1508857/1‑69 (MQ=255) ggTTAATGCCGAGAAGTCGACTAATTTGTTTTCCAGCGTGTCGAgg < 1:2437347/46‑1 (MQ=39) ggTTAATGCCGAGAAGTCGACTAATTTGTTTTCCAGCGTGTCGAgg < 1:91766/46‑1 (MQ=39) ggTTAATGCCGAGAAGTCGACTAATTTGTTTTCCAGCGTGTCGAgg < 1:881730/46‑1 (MQ=39) ggTTAATGCCGAGAAGTCGACTAATTTGTTTTCCAGCGTGTCGAgg < 1:707779/46‑1 (MQ=39) ggTTAATGCCGAGAAGTCGACTAATTTGTTTTCCAGCGTGTCGAgg < 1:661456/46‑1 (MQ=39) ggTTAATGCCGAGAAGTCGACTAATTTGTTTTCCAGCGTGTCGAgg < 1:576827/46‑1 (MQ=39) ggTTAATGCCGAGAAGTCGACTAATTTGTTTTCCAGCGTGTCGAgg < 1:3244871/46‑1 (MQ=39) ggTTAATGCCGAGAAGTCGACTAATTTGTTTTCCAGCGTGTCGAgg < 1:3026917/46‑1 (MQ=39) ggTTAATGCCGAGAAGTCGACTAATTTGTTTTCCAGCGTGTCGAgg < 1:2949525/46‑1 (MQ=39) ggTTAATGCCGAGAAGTCGACTAATTTGTTTTCCAGCGTGTCGAgg < 1:2803473/46‑1 (MQ=39) ggTTAATGCCGAGAAGTCGACTAATTTGTTTTCCAGCGTGTCGAgg < 1:2770315/46‑1 (MQ=39) ggTTAATGCCGAGAAGTCGACTAATTTGTTTTCCAGCGTGTCGAgg < 1:2746360/46‑1 (MQ=39) ggTTAATGCCGAGAAGTCGACTAATTTGTTTTCCAGCGTGTCGAgg < 1:2315030/46‑1 (MQ=39) ggTTAATGCCGAGAAGTCGACTAATTTGTTTTCCAGCGTGTCGAgg < 1:1827636/46‑1 (MQ=39) ggTTAATGCCGAGAAGTCGACTAATTTGTTTTCCAGCGTGTCGAgg < 1:1806707/46‑1 (MQ=39) ggTTAATGCCGAGAAGTCGACTAATTTGTTTTCCAGCGTGTCGAgg < 1:1802721/46‑1 (MQ=39) ggTTAATGCCGAGAAGTCGACTAATTTGTTTTCCAGCGTGTCGAgg < 1:173158/46‑1 (MQ=39) ggTTAATGCCGAGAAGTCGACTAATTTGTTTTCCAGCGTGTCGAgg < 1:1700897/46‑1 (MQ=39) ggTTAATGCCGAGAAGTCGACTAATTTGTTTTCCAGCGTGTCGAgg < 1:1478945/46‑1 (MQ=39) ggTTAATGCCGAGAAGTCGACTAATTTGTTTTCCAGCGTGTCGAgg < 1:1428507/46‑1 (MQ=39) ggTTAATGCCGAGAAGTCGACTAATTTGTTTTCCAGCGTGTCGAgg < 1:1321034/46‑1 (MQ=39) ggTTAATGCCGAGAAGTCGACTAATTTGTTTTCCAGCGTGTCGAgg < 1:1097156/46‑1 (MQ=39) gTTAATGCCGAGAAGTCGACTAATTTGTTTTCCAGCGTGTCGAgg < 1:2676891/45‑1 (MQ=38) ttAACGCCGAGAAGTCGACTAATTTGCTTTCCAGCGTGTCGAGGGCTTGCGCAACGTCGGCCTGCGGTg < 1:785320/69‑1 (MQ=255) | GGGTGCCAAAATCCATCAGATGGAAGCGCGACATATCAAGACCGGCGGTTAACGCCGAGAAGTCGACTAATTTGCTTTCCAGCGTGTCGAGGGCTTGCGCAACGTCGGCCTGCGGTG > minE/646527‑646643 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |