| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 646,754 | G→A | 22.6% | S101F (TCC→TTC) | pncB ← | nicotinate phosphoribosyltransferase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 646,754 | 0 | G | A | 22.6% | 89.5 / 17.9 | 66 | S101F (TCC→TTC) | pncB | nicotinate phosphoribosyltransferase |
| Reads supporting (aligned to +/- strand): ref base G (26/25); new base A (10/5); total (36/30) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 3.80e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.10e-01 | |||||||||||
AACTTCCCAGAGGATGACTTCACGCCACGGGCCGCTTAAACGAATATCCAGCTTGCCATTATCGTTGGACACGGTGACTTGTTCCGGGTTAAAGCGGAACTCGCGTAACCAGTTAAGATAGTCG > minE/646687‑646810 | aaCTTCCCAGAGGATGACTTCACGCCACGGGCCGCTTAAACGAATATCCAGCTTGCCATTATCGTTGGa < 1:2489079/69‑1 (MQ=255)aaCTTCCCAGAGGATGACTTCACGCCACGGGCCGCTTAAACGAATATCCAGCTTGCCATTATCGTTGGa < 1:234247/69‑1 (MQ=255)aaCTTCCCAGAGGATGACTTCACGCCACGGGCCGCTTAAACGAATATCCAGCTTGCCATTATCGTTGGa < 1:1796635/69‑1 (MQ=255)aaCTTCCCAGAGGATGACTTCACGCCACGGGCCGCTTAAACGAATATCCAGCTTGCCATTATCGTTGGa < 1:1800696/69‑1 (MQ=255)aaCTTCCCAGAGGATGACTTCACGCCACGGGCCGCTTAAACGAATATCCAGCTTGCCATTATCGTTGGa < 1:2832304/69‑1 (MQ=255)aaCTTCCCAGAGGATGACTTCACGCCACGGGCCGCTTAAACGAATATCCAGCTTGCCATTATCGTTGGa < 1:1867450/69‑1 (MQ=255)aaCTTCCCAGAGGATGACTTCACGCCACGGGCCGCTTAAACGAATATCCAGCTTGCCATTATCGTTGGa < 1:3142783/69‑1 (MQ=255) tCCCAGAGGATGACTTCACGCCACGGGCCGCTTAAACGAATATCCAGCTTGCTATTATCGTTGAac > 1:3352448/1‑66 (MQ=255) tCCCAGAGGATGACTTCACGCCACGGGCCGCTTAAACGAATATCCAGCTTGCCATTATCGTTGAac > 1:1159223/1‑66 (MQ=255) tCCCAGAGGATGACTTCACGCCACGGGCCGCTTAAACGAATATCCAGCTTGCCATTATCGTTGAac < 1:2670653/66‑1 (MQ=255) tCCCAGAGGATGACTTCACGCCACGGGCCGCTTAAACGAATATCCAGCTTGCCATTATCGTTGAac > 1:2739165/1‑66 (MQ=255) tCCCAGAGGATGACTTCACGCCACGGGCCGCTTAAACGAATATCCAGCTTGCCATTATCGTTGAac > 1:2739562/1‑66 (MQ=255) tCCCAGAGGATGACTTCACGCCACGGGCCGCTTAAACGAATATCCAGCTTGCCATTATCGTTGAac < 1:2423391/66‑1 (MQ=255) tCCCAGAGGATGACTTCACGCCACGGGCCGCTTAAACGAATATCCAGCTTGCCATTATCGTTGAac < 1:2969337/66‑1 (MQ=255) tCCCAGAGGATGACTTCACGCCACGGGCCGCTTAAACGAATATCCAGCTTGCCATTATCGTTGAac < 1:3101614/66‑1 (MQ=255) tCCCAGAGGATGACTTCACGCCACGGGCCGCTTAAACGAATATCCAGCTTGCCATTATCGTTGAac > 1:3229277/1‑66 (MQ=255) tCCCAGAGGATGACTTCACGCCACGGGCCGCTTAAACGAATATCCAGCTTGCCATTATCGTTGAac > 1:34966/1‑66 (MQ=255) tCCCAGAGGATGACTTCACGCCACGGGCCGCTTAAACGAATATCCAGCTTGCCATTATCGTTGAac > 1:1992288/1‑66 (MQ=255) tCCCAGAGGATGACTTCACGCCACGGGCCGCTTAAACGAATATCCAGCTTGCCATTATCGTTGAac > 1:38913/1‑66 (MQ=255) tCCCAGAGGATGACTTCACGCCACGGGCCGCTTAAACGAATATCCAGCTTGCCATTATCGTTGAac < 1:1192386/66‑1 (MQ=255) tCCCAGAGGATGACTTCACGCCACGGGCCGCTTAAACGAATATCCAGCTTGCCATTATCGTTGAac > 1:2043509/1‑66 (MQ=255) tCCCAGAGGATGACTTCACGCCACGGGCCGCTTAAACGAATATCCAGCTTGCCATTATCGTTGAac > 1:749674/1‑66 (MQ=255) cACGCCACGGGCCGCTTAAACGAATATCCAGCTTGCCATTATCGTTGGACACGGTGACTTGTTCCggg > 1:1728396/1‑68 (MQ=255) cACGCCACGGGCCGCTTAAACGAATATCCAGCTTGCCATTATCGTTGGACACGGTGACTTGTTCCggg > 1:2487483/1‑68 (MQ=255) cACGCCACGGGCCGCTTAAACGAATATCCAGCTTGCCATTATCGTTGGACACGGTGACTTGTTCCggg > 1:1846369/1‑68 (MQ=255) cACGCCACGGGCCGCTTAAACGAATATCCAGCTTGCCATTATCGTTGGACACGGTGACTTGTTCCggg > 1:3032007/1‑68 (MQ=255) cACGCCACGGGCCGCTTAAACGAATATCCAGCTTGCCATTATCGTTGGACACGGTGACTTGTGCCggg > 1:229597/1‑68 (MQ=255) gggCCGCTTAAACGAATATCCAGCTTGCCATTATCGTTGGa < 1:2660698/41‑1 (MQ=255) tAAACGAATATCCAGCTTGCCATTATCGTTGGACACGGTGACTTGTTCCGGGTTAAAGCgg > 1:695411/1‑61 (MQ=255) tAAACGAATATCCAGCTTGCCATTATCGTTGGACACGGTGACTTGTTCCGGGTTAAAGCgg > 1:789639/1‑61 (MQ=255) tAAACGAATATCCAGCTTGCCATTATCGTTGGACACGGTGACTTGTTCCGGGTTAAAGCgg > 1:932737/1‑61 (MQ=255) tAAACGAATATCCAGCTTGCCATTATCGTTGGACACGGTGACTTGTTCCGGGTTAAAGCgg > 1:99643/1‑61 (MQ=255) tAAACGAATATCCAGCTTGCCATTATCGTTGGACACGGTGACTTGTTCCGGGTTAAAGCgg > 1:2661465/1‑61 (MQ=255) tAAACGAATATCCAGCTTGCCATTATCGTTGGACACGGTGACTTGTTCCGGGTTAAAGCgg > 1:2657440/1‑61 (MQ=255) tAAACGAATATCCAGCTTGCCATTATCGTTGGACACGGTGACTTGTTCCGGGTTAAAGCgg > 1:2017150/1‑61 (MQ=255) tAAACGAATATCCAGCTTGCCATTATCGTTGGACACGGTGACTTGTTCCGGGTTAAAGCgg < 1:2571261/61‑1 (MQ=255) tAAACGAATATCCAGCTTGCCATTATCGTTGGACACGGTGACTTGTTCCGGGTTAAAGCgg > 1:1446095/1‑61 (MQ=255) tAAACGAATATCCAGCTTGCCATTATCGTTGGACACGGTGACTTGTTCCGGGTTAAAGCgg > 1:1551349/1‑61 (MQ=255) tAAACGAATATCCAGCTTGCCATTATCGTTGGACACGGTGACTTGTTCCGGGTTAAAGCgg > 1:2093595/1‑61 (MQ=255) tAAACGAATATCCAGCTTGCCATTATCGTTGGACACGGTGACTTGTTCCGGGTTAAAGCgg > 1:1991042/1‑61 (MQ=255) tAAACGAATATCCAGCTTGCCATTATCGTTGGACACGGTGACTTGTTCCGGGTTAAAGCgg > 1:2245786/1‑61 (MQ=255) cAGCTTGCCATTATCGTTGGACACGGTGACTTGTTCCGGGTTAAAGCGGAACTCGCGTAACCAGTTaa > 1:2580363/1‑68 (MQ=255) cAGCTTGCCATTATCGTTGGACACGGTGACTTGTTCCGGGTTAAAGCGGAACTCGCGTAACCAGTTaa > 1:2079161/1‑68 (MQ=255) cAGCTTGCCATTATCGTTGGACACGGTGACTTGTTCCGGGTTAAAGCGGAACTCGCGTAACCAGTTaa > 1:3313095/1‑68 (MQ=255) cAGCTTGCCATTATCGTTGGACACGGTGACTTGTTCCGGGTTAAAGCGGAACTCGCGTAACCAGTTaa > 1:2094552/1‑68 (MQ=255) cAGCTTGCCATTATCGTTGGACACGGTGACTTGTTCCGGGTTAAAGCGGAACTCGCGTAACCAGTTaa > 1:3106560/1‑68 (MQ=255) cAGCTTGCCATTATCGTTGGACACGGTGACTTGTTCCGGGTTAAAGCGGAACTCGCGTAACCAGTTaa > 1:689318/1‑68 (MQ=255) cAGCTTGCCATTATCGTTGGACACGGTGACTTGTTCCGGGTTAAAGCGGAACTCGCGTAACCAGTTaa > 1:2916989/1‑68 (MQ=255) cAGCTTGCCATTATCGTTGGACACGGTGACTTGTTCCGGGTTAAAGCGGAACTCGCGTAACCAGTTaa > 1:225213/1‑68 (MQ=255) cAGCTTGCCATTATCGTTGGACACGGTGACTTGTTCCGGGTTAAAGCGGAACTCGCGTAACCAGTTaa > 1:78243/1‑68 (MQ=255) gCTTGCCATTATCGTTGGACACGGTGACTTGTTCCGGGTTAAAGCGGAACTCGCGTAACCAGTTAAGAt < 1:1833301/69‑1 (MQ=255) gCTTGCCATTATCGTTGGACACGGTGACTTGTTCCGGGTTAAAGCGGAACTCGCGTAACCAGTTAAGAt < 1:175833/69‑1 (MQ=255) gCTTGCCATTATCGTTGGACACGGTGACTTGTTCCGGGTTAAAGCGGAACTCGCGTAACCAGTTAAGAt < 1:412876/69‑1 (MQ=255) gCTTGCCATTATCGTTGGACACGGTGACTTGTTCCGGGTTAAAGCGGAACTCGCGTAACCAGTTAAGAt < 1:261428/69‑1 (MQ=255) gCTTGCCATTATCGTTGGACACGGTGACTTGTTCCGGGTTAAAGCGGAACTCGCGTAACCAGTTAAGAt < 1:1143931/69‑1 (MQ=255) gCTTGCCATTATCGTTGGACACGGTGACTTGTTCCGGGTTAAAGCGGAACTCGCGTAACCAGTTAAGAt < 1:3166570/69‑1 (MQ=255) gCTTGCCATTATCGTTGGACACGGTGACTTGTTCCGGGTTAAAGCGGAACTCGCGTAACCAGTTAAGAt < 1:2096557/69‑1 (MQ=255) gCTTGCCATTATCGTTGGACACGGTGACTTGTTCCGGGTTAAAGCGGAACTCGCGTAACCAGTTAAGAt < 1:2507137/69‑1 (MQ=255) gCTTGCCATTATCGTTGGACACGGTGACTTGTTCCGGGTTAAAGCGGAACTCGCGTAACCAGTTAAGAt < 1:2558926/69‑1 (MQ=255) cTTGCCATTATCGTTGGACACGGTGACTTGTTCCGGGTTAAAGCGGAACTCGCGTAACCAGTTAAGATa < 1:685449/69‑1 (MQ=255) cTTGCCATTATCGTTGGACACGGTGACTTGTTCCGGGTTAAAGCGGAACTCGCGTAACCAGTTAAGATa < 1:3083368/69‑1 (MQ=255) cTTGCCATTATCGTTGGACACGGTGACTTGTTCCGGGTTAAAGCGGAACTCGCGTAACCAGTTAAGATa < 1:2791228/69‑1 (MQ=255) cTTGCCATTATCGTTGGACACGGTGACTTGTTCCGGGTTAAAGCGGAACTCGCGTAACCAGTTAAGATa < 1:2425560/69‑1 (MQ=255) cTTGCCATTATCGTTGGACACGGTGACTTGTTCCGGGTTAAAGCGGAACTCGCGTAACCAGTTAAGATa < 1:914699/69‑1 (MQ=255) cATTATCGTTGGACACGGTGACTTGTTCCGGGTTAAAGCGGAACTCGCGTAACCAGTTAAGATAGTCg < 1:2105767/68‑1 (MQ=255) cGTTGGACACGGTGACTTGTTCCGGGTTAAAGCGGAACTCGCGTAACCAGTTAAGAt < 1:318898/57‑1 (MQ=255) | AACTTCCCAGAGGATGACTTCACGCCACGGGCCGCTTAAACGAATATCCAGCTTGCCATTATCGTTGGACACGGTGACTTGTTCCGGGTTAAAGCGGAACTCGCGTAACCAGTTAAGATAGTCG > minE/646687‑646810 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |