Predicted mutation
evidence seq id position mutation freq annotation gene description
RA minE 68,549 T→G 46.2% intergenic (‑101/‑559) leuL ← / → leuO leu operon leader peptide/DNA‑binding transcriptional activator

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*minE68,5490TG46.2% 2.7 / 88.7 78intergenic (‑101/‑559)leuL/leuOleu operon leader peptide/DNA‑binding transcriptional activator
Reads supporting (aligned to +/- strand):  ref base T (24/18);  new base G (21/15);  total (45/33)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GTTACTGGATACAAAAACGGATGTCAACCCTGACGCAATAAAAACGTCCCGCCAGCGTGAGTTCTGCATCCGTAAAATTAGCTAATTGTGCTGCGGTGGTTAAAGTAAGCGATATTAATTTCTGCTTAACTA  >  minE/68485‑68616
                                                                |                                                                   
gTTACTGGATACAAAAACGGATGTCAACCCTGACGCAATAAAAACGTCCCGCCAGCGTGAGTTCTGCAt                                                                 >  1:3342424/1‑69 (MQ=255)
gTTACTGGATACAAAAACGGATGTCAACCCTGACGCAATAAAAACGTCCCGCCAGCGTGAGTTCTGCAt                                                                 >  1:801652/1‑69 (MQ=255)
gTTACTGGATACAAAAACGGATGTCAACCCTGACGCAATAAAAACGTCCCGCCAGCGTGAGTTCTGCAt                                                                 >  1:303947/1‑69 (MQ=255)
 ttACTGGATACAAAAACGGATGTCAACCCTGACGCAATAAAAACGTCCCGCCAGCGTGAGTTCTGCATc                                                                >  1:2655027/1‑69 (MQ=255)
       gATACAAAAACGGATGTCAACCCTGAAGCAATAAAAACGTCACGCCATTGTGAGTTCGGCATCCGTaaa                                                          >  1:2642834/1‑69 (MQ=255)
       gATACAAAAACGGATGTCAACCCTGAAGCAATAAAAACGTCACGCCATTGTGAGTTCGGCATCCGTaaa                                                          >  1:1852527/1‑69 (MQ=255)
       gATACAAAAACGGATGTCAACCCTGAAGCAATAAAAACGTCACGCCATTGTGAGTTCGGCATCCGTaaa                                                          >  1:1884093/1‑69 (MQ=255)
       gATACAAAAACGGATGTCAACCCTGAAGCAATAAAAACGTCACGCCATTGTGAGTTCGGCATCCGTaaa                                                          >  1:2449580/1‑69 (MQ=255)
       gATACAAAAACGGATGTCAACCCTGAAGCAATAAAAACGTCACGCCATTGTGAGTTCGGCATCCGTaaa                                                          >  1:2461862/1‑69 (MQ=255)
       gATACAAAAACGGATGTCAACCCTGAAGCAATAAAAACGTCACGCCATTGTGAGTTCGGCATCCGTaaa                                                          >  1:2476925/1‑69 (MQ=255)
       gATACAAAAACGGATGTCAACCCTGAAGCAATAAAAACGTCACGCCATTGTGAGTTCGGCATCCGTaaa                                                          >  1:800151/1‑69 (MQ=255)
       gATACAAAAACGGATGTCAACCCTGAAGCAATAAAAACGTCACGCCATTGTGAGTTCGGCATCCGTaaa                                                          >  1:2557701/1‑69 (MQ=255)
       gATACAAAAACGGATGTCAACCCTGAAGCAATAAAAACGTCACGCCATTGTGAGTTCGGCATCCGTaaa                                                          >  1:63770/1‑69 (MQ=255)
       gATACAAAAACGGATGTCAACCCTGAAGCAATAAAAACGTCACGCCATTGTGAGTTCGGCATCCGTaaa                                                          >  1:2842997/1‑69 (MQ=255)
       gATACAAAAACGGATGTCAACCCTGAAGCAATAAAAACGTCACGCCATTGTGAGTTCGGCATCCGTaaa                                                          >  1:3132536/1‑69 (MQ=255)
       gATACAAAAACGGATGTCAACCCTGAAGCAATAAAAACGTCACGCCATTGTGAGTTCGGCATCCGTaaa                                                          >  1:3132557/1‑69 (MQ=255)
       gATACAAAAACGGATGTCAACCCTGAAGCAATAAAAACGTCACGCCATTGTGAGTTCGGCATCCGTaaa                                                          >  1:36818/1‑69 (MQ=255)
       gATACAAAAACGGATGTCAACCCTGAAGCAATAAAAACGTCACGCCATTGTGAGTTCGGCATCCGTaaa                                                          >  1:497358/1‑69 (MQ=255)
       gATACAAAAACGGATGTCAACCCTGAAGCAATAAAAACGTCACGCCATTGTGAGTTCGGCATCCGTaaa                                                          >  1:982330/1‑69 (MQ=255)
       gATACAAAAACGGATGTCAACCCTGAAGCAATAAAAACGTCACGCCATTGTGAGTTCGGCATCCGTaaa                                                          >  1:868393/1‑69 (MQ=255)
       gATACAAAAACGGATGTCAACCCTGAAGCAATAAAAACGTCACGCCATTGTGAGTTCGGCATCCGTaaa                                                          >  1:1598495/1‑69 (MQ=255)
       gATACAAAAACGGATGTCAACCCTGAAGCAATAAAAACGTCACGCCATTGTGAGTTCGGCATCCGTaaa                                                          >  1:1721695/1‑69 (MQ=255)
       gATACAAAAACGGATGTCAACCCTGAAGCAATAAAAACGTCACGCCATTGTGAGTTCGGCATCCGTaa                                                           >  1:1080790/1‑68 (MQ=255)
       gATACAAAAACGGATGTCAACCCTGAAGCAATAAAAACGTCACGCCATTGCGAGTTCGGCATCCGTaaa                                                          >  1:1744236/1‑69 (MQ=255)
       gATACAAAAACGGATGTCAACCCTGAAGCAAGAAAAACGTCACGCCATTGTGAGTTCGGCATCCGTaaa                                                          >  1:1777870/1‑69 (MQ=255)
              aaaCGGATGTCAACCCTGACGCAATAAAAACGTCCCGCCAGCGTGAGTTCTGCATCCGTAAAATTAGCt                                                   <  1:187118/69‑1 (MQ=255)
              aaaCGGATGTCAACCCTGACGCAATAAAAACGTCCCGCCAGCGTGAGTTCTGCATCCGTAAAATTAGCt                                                   <  1:2607044/69‑1 (MQ=255)
                       tCAACCCTGACGCAATAAAAACGTCCCGCCAGCGTGAGTTCTGCATCCGTAAAATTAGCTAATTGtgct                                          >  1:1875003/1‑69 (MQ=255)
                       tCAACCCTGACGCAATAAAAACGTCCCGCCAGCGTGAGTTCTGCATCCGTAAAATTAGCTAATTGtgct                                          >  1:71136/1‑69 (MQ=255)
                                  gCAATAAAAACGTCCCGCCAGCGTGAGTTCTGCATCCGTAAAATTAGCTAATTGTGCTGCGGTGGTTaa                               >  1:1788881/1‑69 (MQ=255)
                                  gCAATAAAAACGTCACGCCATTGTGAGTTCGGCATCCGTAAAATTAGCTAATTGTGCTGCGGTGGtt                                 <  1:2232987/67‑1 (MQ=255)
                                  gCAATAAAAACGTCACGCCATTGTGAGTTCGGCATCCGTAAAATTAGCTAATTGTGCTGCGGTGGtt                                 <  1:967850/67‑1 (MQ=255)
                                  gCAATAAAAACGTCACGCCATTGTGAGTTCGGCATCCGTAAAATTAGCTAATTGTGCTGCGGTGGtt                                 <  1:1338833/67‑1 (MQ=255)
                                  gCAATAAAAACGTCACGCCATTGTGAGTTCGGCATCCGTAAAATTAGCTAATTGTGCTGCGGTGGtt                                 <  1:3051325/67‑1 (MQ=255)
                                  gCAATAAAAACGTCACGCCATTGTGAGTTCGGCATCCGTAAAATTAGCTAATTGTGCTGCGGTGGtt                                 <  1:2952537/67‑1 (MQ=255)
                                  gCAATAAAAACGTCACGCCATTGTGAGTTCGGCATCCGTAAAATTAGCTAATTGTGCTGCGGTGGtt                                 <  1:3341443/67‑1 (MQ=255)
                                  gCAATAAAAACGTCACGCCATTGTGAGTTCGGCATCCGTAAAATTAGCTAATTGTGCTGCGGTGGtt                                 <  1:828612/67‑1 (MQ=255)
                                  gCAATAAAAACGTCACGCCATTGTGAGTTCGGCATCCGTAAAATTAGCTAATTGTGCTGCGGTGGtt                                 <  1:786668/67‑1 (MQ=255)
                                  gCAATAAAAACGTCACGCCATTGTGAGTTCGGCATCCGTAAAATTAGCTAATTGTGCTGCGGTGGtt                                 <  1:1525317/67‑1 (MQ=255)
                                  gCAATAAAAACGTCACGCCATTGTGAGTTCGGCATCCGTAAAATTAGCTAATTGTGCTGCGGTGGtt                                 <  1:1580840/67‑1 (MQ=255)
                                  gCAATAAAAACGTCACGCCATTGTGAGTTCGGCATCCGTAAAATTAGCTAATTGTGCTGCGGTGGtt                                 <  1:1135993/67‑1 (MQ=255)
                                  gCAATAAAAACGTCACGCCATTGTGAGTTCGGCATCCGTAAAATTAGCTAATTGTGCTGCGGTGGtt                                 <  1:2568895/67‑1 (MQ=255)
                                  gCAATAAAAACGTCACGCCATTGTGAGTTCGGCATCCGTAAAATTAGCTAATTGTGCTGCGGTGGtt                                 <  1:2005176/67‑1 (MQ=255)
                                  gCAATAAAAACGTCACGCCATTGTGAGTTCGGCATCCGTAAAATTAGCTAATTGTGCTGCGGTGGtt                                 <  1:1670640/67‑1 (MQ=255)
                                  gCAATAAAAACGTCACGCCATTGTGAGTTCGGCATCCGTAAAATTAGCTAATTGTGCTGCGGTGGtt                                 <  1:230214/67‑1 (MQ=255)
                                   cAATAAAAACGTCCCGCCAGCGTGAGTTCTGCATCCGTAAAAt                                                        >  1:3139941/1‑43 (MQ=255)
                                   cAATAAAAACGTCCCGCCAGCGTGAGTTCTGCATCCGTAAAATTAGCTAATTGTGCTGCGGTGGTTaa                               >  1:2470028/1‑68 (MQ=255)
                                   cAATAAAAACGTCCCGCCAGCGTGAGTTCTGCATCCGTAAAATTAGCTAATTGTGCTGCGGTGGTTaa                               >  1:2138203/1‑68 (MQ=255)
                                   cAATAAAAACGTCCCGCCAGCGTGAGTTCTGCATCCGTAAAATTAGCTAATTGTGCTGCGGTGGTTaa                               >  1:631784/1‑68 (MQ=255)
                                      tAAAAACGTCCCGCCAGCGTGAGTTCTGCATCCGTAAAATTAGCTAATTGTGCTGCGGTGGTTAAAGTa                           <  1:253723/69‑1 (MQ=255)
                                       aaaaaCGTCCCGCCAGCGTGAGTTCTGCATCCGTAAAATTAGCTAATTGTGCTGCGGTGGTTa                                >  1:819336/1‑63 (MQ=255)
                                        aaaaCGTCCCGCCAGCGTGAGTTCTGCATCCGTAAAATTAGCTAATTGTGCTGCGGTGGTTAAAGt                            <  1:201755/66‑1 (MQ=255)
                                        aaaaCGTCCCGCCAGCGTGAGTTCTGCATCCGTAAAATTAGCTAATTGTGCTGCGGTGGTTAAAGt                            <  1:1921869/66‑1 (MQ=255)
                                        aaaaCGTCCCGCCAGCGTGAGTTCTGCATCCGTAAAATTAGCTAATTGTGCTGCGGTGGTTAAAGt                            <  1:2307890/66‑1 (MQ=255)
                                        aaaaCGTCCCGCCAGCGTGAGTTCTGCATCCGTAAAATTAGCTAATTGTGCTGCGGTGGTTAAAGTaa                          >  1:601306/1‑68 (MQ=255)
                                        aaaaCGTCCCGCCAGCGTGAGTTCTGCATCCGTAAAATTAGCTAATTGTGCTGCGGTGGTTAAAGTaa                          >  1:2814519/1‑68 (MQ=255)
                                           aCGTCCCGCCAGCGTGAGTTCTGCATCCGTAAAATTAGCTAATTTTGCTGCGTTGGTTAAAGTAAGCGa                      <  1:2611997/69‑1 (MQ=255)
                                           aCGTCCCGCCAGCGTGAGTTCTGCATCCGTAAAATTAGCTAATTGTGCTGCGGTGGTTAAAGTAAGCGa                      <  1:2853950/69‑1 (MQ=255)
                                           aCGTCCCGCCAGCGTGAGTTCTGCATCCGTAAAATTAGCTAATTGTGCTGCGGTGGTTAAAGTAAGCGa                      <  1:2792149/69‑1 (MQ=255)
                                           aCGTCCCGCCAGCGTGAGTTCTGCATCCGTAAAATTAGCTAATTGTGCTGCGGTGGTTAAAGTAAGCGa                      <  1:451793/69‑1 (MQ=255)
                                           aCGTCCCGCCAGCGTGAGTTCTGCATCCGTAAAATTAGCTAATTGTGCTGCGGTGGTTAAAGTAAGCGa                      <  1:1672158/69‑1 (MQ=255)
                                           aCGTCCCGCCAGCGTGAGTTCTGCATCCGTAAAATTAGCTAATTGTGCTGCGGTGGTTAAAGTAAGCGa                      <  1:524733/69‑1 (MQ=255)
                                           aCGTCCCGCCAGCGTGAGTTCTGCATCCGTAAAATTAGCTAATTGTGCTGCGGTGGTTAAAGTAAGCGa                      <  1:171691/69‑1 (MQ=255)
                                           aCGTCCCGCCAGCGTGAGTTCTGCATCCGTAAAATTAGCTAATTGTGCTGCGGTGGTTAAAGTAAGCGa                      <  1:2384372/69‑1 (MQ=255)
                                           aCGTCCCGCCAGCGTGAGTTCTGCATCCGTAAAATTAGCTAATTGTGCTGCGGTGGTTAAAGTAAGCGa                      <  1:2368543/69‑1 (MQ=255)
                                                        gTGAGTTCTGCATCCGTAAAATTAGCTAATTGTGCTGCGGTTGTTAAAGt                            <  1:2187221/50‑1 (MQ=255)
                                                        gTGAGTTCTGCATCCGTAAAATTAGCTAATTGTGCTGCGGTGGTTAAAGTAAGCGATATTAATTTCTg          >  1:1102323/1‑68 (MQ=255)
                                                        gTGAGTTCTGCATCCGTAAAATTAGCTAATTGTGCTGCGGTGGTTAAAGTAAGCGATATTAATTTCTg          >  1:529102/1‑68 (MQ=255)
                                                          gAGTTCTGCATCCGTAAAATTAGCTAATTGTGCTGCGGTGGTTAAAGTAAGCGATATTAATTTCTGCtt       <  1:1136216/69‑1 (MQ=255)
                                                          gAGTTCTGCATCCGTAAAATTAGCTAATTGTGCTGCGGTGGTTAAAGTAAGCGATATTAATTTCTGCtt       <  1:997619/69‑1 (MQ=255)
                                                           aGTTCTGCATCCGTAAAATTAGCTAATTGTGCTGCGGTGGTTAAAGTAAGCGATATTAATTTCTGCTTa      >  1:3394637/1‑69 (MQ=255)
                                                           aGTTCTGCATCCGTAAAATTAGCTAATTGTGCTGCGGTGGTTAAAGTAAGCGATATTAATTTCTGCTTa      >  1:813129/1‑69 (MQ=255)
                                                           aGTTCTGCATCCGTAAAATTAGCTAATTGTGCTGCGGTGGTTAAAGTAAGCGATATTAATTTCTGCTTa      >  1:1282137/1‑69 (MQ=255)
                                                               cTGCATCCGTAAAATTAGCTAATTGTGCTGCGGTGGTTAAAGTAAGCGATATTAATTTCTGCTTAACt   >  1:2493877/1‑68 (MQ=255)
                                                               cTGCATCCGTAAAATTAGCTAATTGTGCTGCGGTGGTTAAAGTAAGCGATATTAATTTCTGCTTAACTa  >  1:2439970/1‑69 (MQ=255)
                                                               cTGCATCCGTAAAATTAGCTAATTGTGCTGCGGTGGTTAAAGTAAGCGATATTAATTTCTGCTTAACTa  >  1:1998174/1‑69 (MQ=255)
                                                               cTGCATCCGTAAAATTAGCTAATTGTGCTGCGGTGGTTAAAGTAAGCGATATTAATTTCTGCTTAACTa  >  1:1437232/1‑69 (MQ=255)
                                                               cTGCATCCGTAAAATTAGCTAATTGTGCTGCGGTGGTTAAAGTAAGCGATATTAATTTCTGCTTAACTa  >  1:1399810/1‑69 (MQ=255)
                                                                |                                                                   
GTTACTGGATACAAAAACGGATGTCAACCCTGACGCAATAAAAACGTCCCGCCAGCGTGAGTTCTGCATCCGTAAAATTAGCTAATTGTGCTGCGGTGGTTAAAGTAAGCGATATTAATTTCTGCTTAACTA  >  minE/68485‑68616

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: