| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 663,694 | T→C | 51.2% | K202K (AAA→AAG) | ycbX ← | predicted 2Fe‑2S cluster‑containing protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 663,694 | 0 | T | C | 51.2% | ‑2.8 / 35.6 | 43 | K202K (AAA→AAG) | ycbX | predicted 2Fe‑2S cluster‑containing protein |
| Reads supporting (aligned to +/- strand): ref base T (5/16); new base C (10/12); total (15/28) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.03e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.91e-01 | |||||||||||
CTGCCGGATGTTTTTGCCCTTTTTCTGGGCTGACGGTGGTGAAAATACAGCGGCTACAAGGTTTAACCACATCAAACACCACATCACCAATGCGAATCACTTTCCAGCGATCTTCTTCCCAG > minE/663633‑663754 | cTGCCGGATGTTTTTGCCCTTTTTCTGGGCTGACGGTGGTGAAAATACAGCGGCTACAAGGTTTAACca < 1:2615275/69‑1 (MQ=255)cTGCCGGATGTTTTTGCCCTTTTTCTGGGCTGACGGTGGTGAAAATACAGCGGCTACAAGGTTTAACca < 1:3011569/69‑1 (MQ=255) ggATGTTTTTGCCCTTTTTCTGGGCTGACGGTGGTGAAAATACAGCGGCTACAAGGTTTAACCACATCa < 1:2376064/69‑1 (MQ=255) ggATGTTTTTGCCCTTTTTCTGGGCTGACGGTGGTGAAAATACAGCGGCTACAAGGTTTAACCACATCa < 1:1882323/69‑1 (MQ=255) ggATGTTTTTGCCCTTTTTCTGGGCTGACGGTGGTGAAAATACAGCGGCTACAAGGTTTAACCACATCa < 1:277896/69‑1 (MQ=255) ggATGTTTTTGCCCTTTTTCTGGGCTGACGGTGGTGAAAATACAGCGGCTACAAGGTTTAACCACATCa < 1:1311071/69‑1 (MQ=255) ggATGTTTTTGCCCTTTTTCTGGGCTGACGGTGGTGAAAATACAGCGGCTACAAGGTTTAACCACATCa < 1:530751/69‑1 (MQ=255) ggCTGACGGTGGTGAAAATACAGCGGCTACAGGGCTTAACCACATCAAACACGACATCACCAATGCGa > 1:1423506/1‑68 (MQ=255) ggCTGACGGTGGTGAAAATACAGCGGCTACAAGGTTTAACCACATCAAACACCACATCACCAATGCGa > 1:3320546/1‑68 (MQ=255) ggCTGACGGTGGTGAAAATACAGCGGCTACAAGGCTTAACCACATCAAACACGACATCACCAATGCGa > 1:1384511/1‑68 (MQ=255) ggCTGACGGTGGTGAAAATACAGCGGCTACAAGGCTTAACCACATCAAACACGACATCACCAATGCGa > 1:2461120/1‑68 (MQ=255) ggCTGACGGTGGTGAAAATACAGCGGCTACAAGGCTTAACCACATCAAACACGACATCACCAATGCGa > 1:1489647/1‑68 (MQ=255) ggCTGACGGTGGTGAAAATACAGCGGCTACAAGGCTTAACCACATCAAACACGACATCACCAATGCGa > 1:1576594/1‑68 (MQ=255) ggCTGACGGTGGTGAAAATACAGCGGCTACAAGGCTTAACCACATCAAACACGACATCACCAATGCGa > 1:1678059/1‑68 (MQ=255) ggCTGACGGTGGTGAAAATACAGCGGCTACAAGGCTTAACCACATCAAACACGACATCACCAATGCGa > 1:3121254/1‑68 (MQ=255) ggCTGACGGTGGTGAAAATACAGCGGCTACAAGGCTTAACCACATCAAACACGACATCACCAATGCGa > 1:368589/1‑68 (MQ=255) ggCTGACGGTGGTGAAAATACAGCGGCTACAAGGCTTAACCACATCAAACACGACATCACCAATGCGa > 1:2837333/1‑68 (MQ=255) ggCTGACGGTGATGAAAATACAGCGGCTACAAGGCTTAACCACATCAAACACGACATCACCAATGCGa > 1:146536/1‑68 (MQ=255) gCTGACGGTGGTGAAAATACAGCGGCTACAAGGTTTAACCACATCAAACACCACATCACCAATGCGAAt > 1:2617200/1‑69 (MQ=255) gCTGACGGTGGTGAAAATACAGCGGCTACAAGGTTTAACCACATCAAACACCACATCACCAATGCGAAt > 1:2914205/1‑69 (MQ=255) gCTGACGGTGGTGAAAATACAGCGGCTACAAGGTTTAACCACATCAAACACCACATCACCAATGCGAAt > 1:1444002/1‑69 (MQ=255) gCTGACGGTGGTGAAAATACAGCGGCTACAAGGTTTAACCACATCAAACACCACATCACCAATGCGAAt > 1:1421852/1‑69 (MQ=255) cTGACGGTGGTGAAAATACAGCGGCTACAAGGTTTAACCACATCAAACACCACATCACCAATGCGAAtg < 1:1405869/69‑2 (MQ=255) cTGACGGTGGTGAAAATACAGCGGCTACAAGGTTTAACCACATCAAACACCACATCACCAATGCGAATc < 1:2737241/69‑1 (MQ=255) tggtgAAAATACAGCGGCTACAAGGTTTAACCACATCAAACACCACATCACCAATGCGAATCACTTTcc < 1:933206/69‑1 (MQ=255) tggtgAAAATACAGCGGCTACAAGGTTTAACCACATCAAACACCACATCACCAATGCGAATCACTTTcc < 1:1277827/69‑1 (MQ=255) gAAAATACAGCGGCTACAAGGTTTAACCACATCAAACACCACATCACCaa < 1:2403902/50‑1 (MQ=255) gAAAATACAGCGGCTACAAGGTTTAACCACATCAAACACCACATCACCaa < 1:2573006/50‑1 (MQ=255) gAAAATACAGCGGCTACAAGGTTTAACCACATCAAACACCACATCACCaa < 1:1301560/50‑1 (MQ=255) gAAAATACAGCGGCTACAAGGTTTAACCACATCAAACACCACATCACCaa < 1:188971/50‑1 (MQ=255) gAAAATACAGCGGCTACAAGGTTTAACCACATCAAACACCACATCACCaa < 1:1813054/50‑1 (MQ=255) cTACAAGGCTTAACCACATCAAACACGACATCACCAATGCGAATCACTTTCCAGCTATCTTCTTCCCAg < 1:1040871/69‑1 (MQ=255) cTACAAGGCTTAACCACATCAAACACGACATCACCAATGCGAATCACTTTCCAGCTATCTTCTTCCCAg < 1:774075/69‑1 (MQ=255) cTACAAGGCTTAACCACATCAAACACGACATCACCAATGCGAATCACTTTCCAGCTATCTTCTTCCCAg < 1:543608/69‑1 (MQ=255) cTACAAGGCTTAACCACATCAAACACGACATCACCAATGCGAATCACTTTCCAGCTATCTTCTTCCCAg < 1:1307981/69‑1 (MQ=255) cTACAAGGCTTAACCACATCAAACACGACATCACCAATGCGAATCACTTTCCAGCTATCTTCTTCCCAg < 1:172113/69‑1 (MQ=255) cTACAAGGCTTAACCACATCAAACACGACATCACCAATGCGAATCACTTTCCAGCTATCTTCTTCCCAg < 1:3173732/69‑1 (MQ=255) cTACAAGGCTTAACCACATCAAACACGACATCACCAATGCGAATCACTTTCCAGCTATCTTCTTCCCAg < 1:3004588/69‑1 (MQ=255) cTACAAGGCTTAACCACATCAAACACGACATCACCAATGCGAATCACTTTCCAGCTATCTTCTTCCCAg < 1:299482/69‑1 (MQ=255) cTACAAGGCTTAACCACATCAAACACGACATCACCAATGCGAATCACTTTCCAGCTATCTTCTTCCCAg < 1:2169564/69‑1 (MQ=255) cTACAAGGCTTAACCACATCAAACACGACATCACCAATGCGAATCACTTTCCAGCTATCTTCTTCCCAg < 1:234976/69‑1 (MQ=255) tACAAGGCTTAACCACATCAAACACGACATCACCAATGCGAATCACTTTCCAGCTATCTTCTTCCCAg < 1:408590/68‑1 (MQ=255) tACAAGGCTTAACCACATCAAACACGACATCACCAATGCGAATCACTTTCCAGCTATCTTCTTCCCAg < 1:490881/68‑1 (MQ=255) | CTGCCGGATGTTTTTGCCCTTTTTCTGGGCTGACGGTGGTGAAAATACAGCGGCTACAAGGTTTAACCACATCAAACACCACATCACCAATGCGAATCACTTTCCAGCGATCTTCTTCCCAG > minE/663633‑663754 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |