| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 663,712 | C→G | 34.9% | V196V (GTG→GTC) | ycbX ← | predicted 2Fe‑2S cluster‑containing protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 663,712 | 0 | C | G | 34.9% | 55.2 / 49.9 | 63 | V196V (GTG→GTC) | ycbX | predicted 2Fe‑2S cluster‑containing protein |
| Reads supporting (aligned to +/- strand): ref base C (22/19); new base G (10/12); total (32/31) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.03e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.73e-01 | |||||||||||
GGCTGACGGTGGTGAAAATACAGCGGCTACAAGGTTTAACCACATCAAACACCACATCACCAATGCGAATCACTTTCCAGCGATCTTCTTCCCAGGCTGACGCGCCGGAAACC > minE/663660‑663772 | ggCTGACGGTGGTGAAAATACAGCGGCTACAGGGCTTAACCACATCAAACACGACATCACCAATGCGa > 1:1423506/1‑68 (MQ=255)ggCTGACGGTGGTGAAAATACAGCGGCTACAAGGTTTAACCACATCAAACACCACATCACCAATGCGa > 1:3320546/1‑68 (MQ=255)ggCTGACGGTGGTGAAAATACAGCGGCTACAAGGCTTAACCACATCAAACACGACATCACCAATGCGa > 1:1384511/1‑68 (MQ=255)ggCTGACGGTGGTGAAAATACAGCGGCTACAAGGCTTAACCACATCAAACACGACATCACCAATGCGa > 1:1489647/1‑68 (MQ=255)ggCTGACGGTGGTGAAAATACAGCGGCTACAAGGCTTAACCACATCAAACACGACATCACCAATGCGa > 1:3121254/1‑68 (MQ=255)ggCTGACGGTGGTGAAAATACAGCGGCTACAAGGCTTAACCACATCAAACACGACATCACCAATGCGa > 1:2461120/1‑68 (MQ=255)ggCTGACGGTGGTGAAAATACAGCGGCTACAAGGCTTAACCACATCAAACACGACATCACCAATGCGa > 1:1576594/1‑68 (MQ=255)ggCTGACGGTGGTGAAAATACAGCGGCTACAAGGCTTAACCACATCAAACACGACATCACCAATGCGa > 1:2837333/1‑68 (MQ=255)ggCTGACGGTGGTGAAAATACAGCGGCTACAAGGCTTAACCACATCAAACACGACATCACCAATGCGa > 1:368589/1‑68 (MQ=255)ggCTGACGGTGGTGAAAATACAGCGGCTACAAGGCTTAACCACATCAAACACGACATCACCAATGCGa > 1:1678059/1‑68 (MQ=255)ggCTGACGGTGATGAAAATACAGCGGCTACAAGGCTTAACCACATCAAACACGACATCACCAATGCGa > 1:146536/1‑68 (MQ=255) gCTGACGGTGGTGAAAATACAGCGGCTACAAGGTTTAACCACATCAAACACCACATCACCAATGCGAAt > 1:1421852/1‑69 (MQ=255) gCTGACGGTGGTGAAAATACAGCGGCTACAAGGTTTAACCACATCAAACACCACATCACCAATGCGAAt > 1:1444002/1‑69 (MQ=255) gCTGACGGTGGTGAAAATACAGCGGCTACAAGGTTTAACCACATCAAACACCACATCACCAATGCGAAt > 1:2914205/1‑69 (MQ=255) gCTGACGGTGGTGAAAATACAGCGGCTACAAGGTTTAACCACATCAAACACCACATCACCAATGCGAAt > 1:2617200/1‑69 (MQ=255) cTGACGGTGGTGAAAATACAGCGGCTACAAGGTTTAACCACATCAAACACCACATCACCAATGCGAAtg < 1:1405869/69‑2 (MQ=255) cTGACGGTGGTGAAAATACAGCGGCTACAAGGTTTAACCACATCAAACACCACATCACCAATGCGAATc < 1:2737241/69‑1 (MQ=255) tggtgAAAATACAGCGGCTACAAGGTTTAACCACATCAAACACCACATCACCAATGCGAATCACTTTcc < 1:933206/69‑1 (MQ=255) tggtgAAAATACAGCGGCTACAAGGTTTAACCACATCAAACACCACATCACCAATGCGAATCACTTTcc < 1:1277827/69‑1 (MQ=255) gAAAATACAGCGGCTACAAGGTTTAACCACATCAAACACCACATCACCaa < 1:2573006/50‑1 (MQ=255) gAAAATACAGCGGCTACAAGGTTTAACCACATCAAACACCACATCACCaa < 1:188971/50‑1 (MQ=255) gAAAATACAGCGGCTACAAGGTTTAACCACATCAAACACCACATCACCaa < 1:1301560/50‑1 (MQ=255) gAAAATACAGCGGCTACAAGGTTTAACCACATCAAACACCACATCACCaa < 1:2403902/50‑1 (MQ=255) gAAAATACAGCGGCTACAAGGTTTAACCACATCAAACACCACATCACCaa < 1:1813054/50‑1 (MQ=255) cTACAAGGCTTAACCACATCAAACACGACATCACCAATGCGAATCACTTTCCAGCTATCTTCTTCCCAg < 1:1040871/69‑1 (MQ=255) cTACAAGGCTTAACCACATCAAACACGACATCACCAATGCGAATCACTTTCCAGCTATCTTCTTCCCAg < 1:234976/69‑1 (MQ=255) cTACAAGGCTTAACCACATCAAACACGACATCACCAATGCGAATCACTTTCCAGCTATCTTCTTCCCAg < 1:299482/69‑1 (MQ=255) cTACAAGGCTTAACCACATCAAACACGACATCACCAATGCGAATCACTTTCCAGCTATCTTCTTCCCAg < 1:3004588/69‑1 (MQ=255) cTACAAGGCTTAACCACATCAAACACGACATCACCAATGCGAATCACTTTCCAGCTATCTTCTTCCCAg < 1:3173732/69‑1 (MQ=255) cTACAAGGCTTAACCACATCAAACACGACATCACCAATGCGAATCACTTTCCAGCTATCTTCTTCCCAg < 1:543608/69‑1 (MQ=255) cTACAAGGCTTAACCACATCAAACACGACATCACCAATGCGAATCACTTTCCAGCTATCTTCTTCCCAg < 1:774075/69‑1 (MQ=255) cTACAAGGCTTAACCACATCAAACACGACATCACCAATGCGAATCACTTTCCAGCTATCTTCTTCCCAg < 1:1307981/69‑1 (MQ=255) cTACAAGGCTTAACCACATCAAACACGACATCACCAATGCGAATCACTTTCCAGCTATCTTCTTCCCAg < 1:2169564/69‑1 (MQ=255) cTACAAGGCTTAACCACATCAAACACGACATCACCAATGCGAATCACTTTCCAGCTATCTTCTTCCCAg < 1:172113/69‑1 (MQ=255) tACAAGGCTTAACCACATCAAACACGACATCACCAATGCGAATCACTTTCCAGCTATCTTCTTCCCAg < 1:408590/68‑1 (MQ=255) tACAAGGCTTAACCACATCAAACACGACATCACCAATGCGAATCACTTTCCAGCTATCTTCTTCCCAg < 1:490881/68‑1 (MQ=255) ttAACCACATCAAACACCACATCACCAATGCGAATCACTTTCCAGCGATCTTCTTCCCAGGCTGAcgcg < 1:1642901/69‑1 (MQ=255) ttAACCACATCAAACACCACATCACCAATGCGAATCACTTTCCAGCGATCTTCTTCCCAGGCTGAcgcg < 1:2887079/69‑1 (MQ=255) acaTCAAACACCACATCACCAATGCGAATCACTTTCCAGCGATCTTCTTCCCAGGCTGACGCGCCGGaa > 1:449323/1‑69 (MQ=255) acaTCAAACACCACATCACCAATGCGAATCACTTTCCAGCGATCTTCTTCCCAGGCTGACGCGCCGGaa > 1:81254/1‑69 (MQ=255) tCAAACACCACATCACCAATGCGAATCACTTTCCAGCGATCTTCTTCCCAGGCTGACGCGCCGGaa < 1:560531/66‑1 (MQ=255) tCAAACACCACATCACCAATGCGAATCACTTTCCAGCGATCTTCTTCCCAGGCTGACGCGCCGGaa > 1:1186824/1‑66 (MQ=255) tCAAACACCACATCACCAATGCGAATCACTTTCCAGCGATCTTCTTCCCAGGCTGACGCGCCGGaa > 1:3022516/1‑66 (MQ=255) tCAAACACCACATCACCAATGCGAATCACTTTCCAGCGATCTTCTTCCCAGGCTGACGCGCCGGaa > 1:575240/1‑66 (MQ=255) tCAAACACCACATCACCAATGCGAATCACTTTCCAGCGATCTTCTTCCCAGGCTGACGCGCCGGaa < 1:504776/66‑1 (MQ=255) tCAAACACCACATCACCAATGCGAATCACTTTCCAGCGATCTTCTTCCCAGGCTGACGCGCCGGaa > 1:1153882/1‑66 (MQ=255) tCAAACACCACATCACCAATGCGAATCACTTTCCAGCGATCTTCTTCCCAGGCTGACGCGCCGGaa < 1:1246982/66‑1 (MQ=255) tCAAACACCACATCACCAATGCGAATCACTTTCCAGCGATCTTCTTCCCAGGCTGACGCGCCGGaa < 1:1005316/66‑1 (MQ=255) tCAAACACCACATCACCAATGCGAATCACTTTCCAGCGATCTTCTTCCCAGGCTGACGCGCCGGaa > 1:939435/1‑66 (MQ=255) tCAAACACCACATCACCAATGCGAATCACTTTCCAGCGATCTTCTTCCCAGGCTGACGCGCCGGaa > 1:1327328/1‑66 (MQ=255) tCAAACACCACATCACCAATGCGAATCACTTTCCAGCGATCTTCTTCCCAGGCTGACGCGCCGGaa > 1:1458195/1‑66 (MQ=255) tCAAACACCACATCACCAATGCGAATCACTTTCCAGCGATCTTCTTCCCAGGCTGACGCGCCGGaa > 1:3000930/1‑66 (MQ=255) tCAAACACCACATCACCAATGCGAATCACTTTCCAGCGATCTTCTTCCCAGGCTGACGCGCCGGaa > 1:1494329/1‑66 (MQ=255) tCAAACACCACATCACCAATGCGAATCACTTTCCAGCGATCTTCTTCCCAGGCTGACGCGCCGGaa > 1:1562184/1‑66 (MQ=255) tCAAACACCACATCACCAATGCGAATCACTTTCCAGCGATCTTCTTCCCAGGCTGACGCGCCGGaa > 1:2758839/1‑66 (MQ=255) tCAAACACCACATCACCAATGCGAATCACTTTCCAGCGATCTTCTTCCCAGGCTGACGCGCCGGaa > 1:179959/1‑66 (MQ=255) tCAAACACCACATCACCAATGCGAATCACTTTCCAGCGATCTTCTTCCCAGGCTGACGCGCCGGaa < 1:1839561/66‑1 (MQ=255) tCAAACACCACATCACCAATGCGAATCACTTTCCAGCGATCTTCTTCCCAGGCTGACGCGCCGGaa > 1:2026184/1‑66 (MQ=255) tCAAACACCACATCACCAATGCGAATCACTTTCCAGCGATCTTCTTCCCAGGCTGACGCGCCGGaa > 1:2037025/1‑66 (MQ=255) tCAAACACCACATCACCAATGCGAATCACTTTCCAGCGATCTTCTTCCCAGGCTGACGCGCCGGaa > 1:2374986/1‑66 (MQ=255) tCAAACACCACATCACCAATGCGAATCACTTTCCAGCGATCTTCTTCCCAGGCTGACGCGCCGGaa < 1:2167546/66‑1 (MQ=255) tCAAACACCACATCACCAATGCGAATCACTTTCCAGCGATCTTCTTCCCAGGCTGACGCGCCGGAAacc < 1:1425045/69‑1 (MQ=255) tCAAACACCACATCACCAATGCGAATCACTTTCCAGCGATCTTCTTCCCAGGCTGACGCGCCGGAAacc < 1:2908759/69‑1 (MQ=255) | GGCTGACGGTGGTGAAAATACAGCGGCTACAAGGTTTAACCACATCAAACACCACATCACCAATGCGAATCACTTTCCAGCGATCTTCTTCCCAGGCTGACGCGCCGGAAACC > minE/663660‑663772 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |