| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 666,267 | A→G | 36.1% | E575E (GAA→GAG) | ycbY → | predicted methyltransferase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 666,267 | 0 | A | G | 36.1% | 17.7 / 18.9 | 36 | E575E (GAA→GAG) | ycbY | predicted methyltransferase |
| Reads supporting (aligned to +/- strand): ref base A (2/21); new base G (7/6); total (9/27) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.93e-03 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
GTGCATGCGGGATTAGGCGGTGCACGCAGCACCACCACCGTGGATATGTCGCGTACTTATCTGGAATGGGCAGAACGCAACCTGCGTCTGAATGGCCTGACCGGGCGTGCGCATCGCCTGATTCAGGCCGATTG > minE/666202‑666335 | gTGCATGCGGGATTAGGCGGTGCACGCAGCACCACCACCGTGGATATGTCGCGTACTTATCTGGAATg > 1:849425/1‑68 (MQ=255) gATTAGGCGGTGCACGCAGCACCACCACCGTGGATATGTCGCGTACTTATCTGGAATGGGCAGAACGCa < 1:1089118/69‑1 (MQ=255) gATTAGGCGGTGCACGCAGCACCACCACCGTGGATATGTCGCGTACTTATCTGGAATGGGCAGAACGCa < 1:2739775/69‑1 (MQ=255) gATTAGGCGGTGCACGCAGCACCACCACCGTGGATATGTCGCGTACTTATCTGGAATGGGCAGAACGCa < 1:1418267/69‑1 (MQ=255) ggTGCACGCAGCACCACCACCGTGGATATGTCGCGTACTTATCTGGAGTGGGCAGAACGCAACCTGc > 1:973914/1‑67 (MQ=255) ggTGCACGCAGCACCACCACCGTGGATATGTCGCGTACTTATCTGGAGTGGGCAGAACGCAACCTGCGt > 1:3048705/1‑69 (MQ=255) ggTGCACGCAGCACCACCACCGTGGATATGTCGCGTACTTATCTGGAGTGGGCAGAACGCAACCTGCGt > 1:1811463/1‑69 (MQ=255) ggTGCACGCAGCACCACCACCGTGGATATGTCGCGTACTTATCTGGAGTGGGCAGAACGCAACCTGCGt > 1:2154503/1‑69 (MQ=255) ggTGCACGCAGCACCACCACCGTGGATATGTCGCGTACTTATCTGGAGTGGGCAGAACGCAACCTGCGt > 1:2724159/1‑69 (MQ=255) ggTGCACGCAGCACCACCACCGTGGATATGTCGCGTACTTATCTGGAGTGGGCAGAACGCAACCTGCGt > 1:487370/1‑69 (MQ=255) ggTGCACGCAGCACCACCACCGTGGATATGTCGCATACTTATCTGGAGTGGGCAGAACGCAACCTGCGt > 1:2658847/1‑69 (MQ=255) gCACGCAGCACCACCACCGTGGATATGTCGCGTACTTATCTGGAGTGGGCAGAACGCAACCTGCGTCTg < 1:3182939/69‑1 (MQ=255) gCACGCAGCACCACCACCGTGGATATGTCGCGTACTTATCTGGAGTGGGCAGAACGCAACCTGCGTCTg < 1:463484/69‑1 (MQ=255) gCACGCAGCACCACCACCGTGGATATGTCGCGTACTTATCTGGAGTGGGCAGAACGCAACCTGCGTCTg < 1:3037507/69‑1 (MQ=255) gCACGCAGCACCACCACCGTGGATATGTCGCGTACTTATCTGGAGTGGGCAGAACGCAACCTGCGTCTg < 1:2031731/69‑1 (MQ=255) gCACGCAGCACCACCACCGTGGATATGTCGCGTACTTATCTGGAGTGGGCAGAACGCAACCTGCGTCTg < 1:220603/69‑1 (MQ=255) gCACGCAGCACCACCACCGTGGATATGTCGCGTACTTATCTGGAGTGGGCAGAACGCAACCTGCGTCTg < 1:2869666/69‑1 (MQ=255) gCACGCAGCACCACCACCGTGGATATGTCGCGTACTTATCTGGAATGGGCAGAACGCAACCTGCGTCTg < 1:3410220/69‑1 (MQ=255) gCACGCAGCACCACCACCGTGGATATGTCGCGTACTTATCTGGAATGGGCAGAACGCAACCTGCGTCTg < 1:295477/69‑1 (MQ=255) gCACGCAGCACCACCACCGTGGATATGTCGCGTACTTATCTGGAATGGGCAGAACGCAACCTGCGTCTg < 1:2877865/69‑1 (MQ=255) gCACGCAGCACCACCACCGTGGATATGTCGCGTACTTATCTGGAATGGGCAGAACGCAACCTGCGTCTg < 1:1144464/69‑1 (MQ=255) gCACGCAGCACCACCACCGTGGATATGTCGCGTACTTATCTGGAATGGGCAGAACGCAACCTGCGTCTg < 1:2271543/69‑1 (MQ=255) gCACGCAGCACCACCACCGTGGATATGTCGCGTACTTATCTGGAATGGGCAGAACGCAACCTGCGTCTg < 1:1861517/69‑1 (MQ=255) gCACGCAGCACCACCACCGTGGATATGTCGCGTACTTATCTGGAATGGGCAGAACGCAACCTGCGTCTg < 1:1737240/69‑1 (MQ=255) gCACGCAGCACCACCACCGTGGATATGTCGCGTACTTATCTGGAATGGGCAGAACGCAACCTGCGTCTg < 1:934201/69‑1 (MQ=255) gCACGCAGCACCACCACCGTGGATATGTCGCGTACTTATCTGGAATGGGCAGAACGCAACCTGCGTCTg < 1:1303202/69‑1 (MQ=255) gATATGTCGCGTACTTATCTGGAATGGGCAGAACGCAACCTGCGTCTGAATGGCCTGACCGGGCGTgcg > 1:1246056/1‑69 (MQ=255) tCGCGTACTTATCTGGAATGGGCAGAACGCAACCTGCGTCTGAATGGCCTGACCGGGCGTGCGCATCGc < 1:3377355/69‑1 (MQ=255) tCGCGTACTTATCTGGAATGGGCAGAACGCAACCTGCGTCTGAATGGCCTGACCGGGCGTGCGCATCGc < 1:446330/69‑1 (MQ=255) tCGCGTACTTATCTGGAATGGGCAGAACGCAACCTGCGTCTGAATGGCCTGACCGGGCGTGCGCATCGc < 1:1185422/69‑1 (MQ=255) tCGCGTACTTATCTGGAATGGGCAGAACGCAACCTGCGTCTGAATGGCCTGACCGGGCGTGCGCATCGc < 1:520759/69‑1 (MQ=255) tCGCGTACTTATCTGGAATGGGCAGAACGCAACCTGCGTCTGAATGGCCTGACCGGGCGTGCGCATCGc < 1:800893/69‑1 (MQ=255) tCGCGTACTTATCTGGAATGGGCAGAACGCAACCTGCGTCTGAATGGCCTGACCGGGCGTGCGCATCGc < 1:2494776/69‑1 (MQ=255) tCGCGTACTTATCTGGAATGGGCAGAACGCAACCTGCGTCTGAATGGCCTGACCGGGCGTGCGCATCGc < 1:1729291/69‑1 (MQ=255) tCGCGTACTTATCTGGAATGGGCAGAACGCAACCTGCGTCTGAATGGCCTGACCGGGCGTGCGCATCGc < 1:1102748/69‑1 (MQ=255) tCGCGTACTTATCTGGAATGGGCAGAACGCAACCTGCGTCTGAATGGCCTGACCGGGCGGGCGCATCGc < 1:75150/69‑1 (MQ=255) aTGGGCAGAACGCAACCTGCGTCTGAATGGCCTGACCGGGCGTGCGCATCGCCTGATTCAGGCCGATTg < 1:3131876/69‑1 (MQ=255) | GTGCATGCGGGATTAGGCGGTGCACGCAGCACCACCACCGTGGATATGTCGCGTACTTATCTGGAATGGGCAGAACGCAACCTGCGTCTGAATGGCCTGACCGGGCGTGCGCATCGCCTGATTCAGGCCGATTG > minE/666202‑666335 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |