| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 667,205 | T→C | 31.9% | D181D (GAT→GAC) | uup → | fused predicted transporter subunits and ATP‑binding components of ABC superfamily |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 667,205 | 0 | T | C | 31.9% | 65.7 / 35.6 | 72 | D181D (GAT→GAC) | uup | fused predicted transporter subunits and ATP‑binding components of ABC superfamily |
| Reads supporting (aligned to +/- strand): ref base T (13/36); new base C (14/9); total (27/45) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 8.45e-03 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.34e-01 | |||||||||||
TAAAGCGGCATTAGGACGCGCGCTGGTGAGTAATCCGCGCGTGCTGTTGCTTGATGAACCGACAAACCACCTGGATATTGAAACCATCGACTGGCTGGAAGGGTTTTTGAA > minE/667151‑667261 | tAAAGCGGCATTAGGACGCGCGCTGGTGAGTAATCCGCGCGTGCTGTTGCTTGATGAACCGACAAacca < 1:2140134/69‑1 (MQ=255)tAAAGCGGCATTAGGACGCGCGCTGGTGAGTAATCCGCGCGTGCTGTTGCTTGATGAACCGACAAacca < 1:213087/69‑1 (MQ=255)tAAAGCGGCATTAGGACGCGCGCTGGTGAGTAATCCGCGCGTGCTGTTGCTTGATGAACCGACAAacca < 1:2383042/69‑1 (MQ=255)tAAAGCGGCATTAGGACGCGCGCTGGTGAGTAATCCGCGCGTGCTGTTGCTTGATGAACCGACAAacca < 1:148241/69‑1 (MQ=255)tAAAGCGGCATTAGGACGCGCGCTGGTGAGTAATCCGCGCGTGCTGTTGCTTGATGAACCGACAAacca < 1:754027/69‑1 (MQ=255)tAAAGCGGCATTAGGACGCGCGCTGGTGAGTAATCCGCGCGTGCTGTTGCTTGATGAACCGACAAacca < 1:93299/69‑1 (MQ=255) ggCATTAGGACGCGCGCTGGTGAGTAATCCGCGCGTGCTGTTGCTTGATGAACCGACAAACCACCTGGa < 1:2278230/69‑1 (MQ=255) ggCATTAGGACGCGCGCTGGTGAGTAATCCGCGCGTGCTGTTGCTTGATGAACCGACAAACCACCTGGa < 1:2085175/69‑1 (MQ=255) ggCATTAGGACGCGCGCTGGTGAGTAATCCGCGCGTGCTGTTGCTTGATGAACCGACAAACCACCTGGa < 1:2082279/69‑1 (MQ=255) ggCATTAGGACGCGCGCTGGTGAGTAATCCGCGCGTGCTGTTGCTTGATGAACCGACAAACCACCTGGa < 1:2078241/69‑1 (MQ=255) ggCATTAGGACGCGCGCTGGTGAGTAATCCGCGCGTGCTGTTGCTTGATGAACCGACAAACCACCTGGa < 1:2218883/69‑1 (MQ=255) ggCATTAGGACGCGCGCTGGTGAGTAATCCGCGCGTGCTGTTGCTTGATGAACCGACAAACCACCTGGa < 1:2910455/69‑1 (MQ=255) ggCATTAGGACGCGCGCTGGTGAGTAATCCGCGCGTGCTGTTGCTTGATGAACCGACAAACCACCTGGa < 1:1901771/69‑1 (MQ=255) ggCATTAGGACGCGCGCTGGTGAGTAATCCGCGCGTGCTGTTGCTTGATGAACCGACAAACCACCTGGa < 1:3066584/69‑1 (MQ=255) ggCATTAGGACGCGCGCTGGTGAGTAATCCGCGCGTGCTGTTGCTTGATGAACCGACAAACCACCTGGa < 1:539736/69‑1 (MQ=255) ggCATTAGGACGCGCGCTGGTGAGTAATCCGCGCGTGCTGTTGCTTGATGAACCGACAAACCACCTGGa < 1:1612659/69‑1 (MQ=255) ggCATTAGGACGCGCGCTGGTGAGTAATCCGCGCGTGCTGTTGCTTGATGAACCGACAAACCACCTGGa < 1:1587939/69‑1 (MQ=255) ggCATTAGGACGCGCGCTGGTGAGTAATCCGCGCGTGCTGTTGCTTGATGAACCGACAAACCACCTGGa < 1:1454586/69‑1 (MQ=255) ggCATTAGGACGCGCGCTGGTGAGTAATCCGCGCGTGCTGTTGCTTGATGAACCGACAAACCACCTGGa < 1:432259/69‑1 (MQ=255) aGGACGCGCGCTGGTGAGTAATCCGCGCGTGCTGTTGCTTGATGAACCGACAAACCACCTGGATATTGa > 1:278094/1‑69 (MQ=255) aGGACGCGCGCTGGTGAGTAATCCGCGCGTGCTGTTGCTTGATGAACCGACAAACCACCTGGATATTGa > 1:2055440/1‑69 (MQ=255) aGGACGCGCGCTGGTGAGTAATCCGCGCGTGCTGTTGCTTGATGAACCGACAAACCACCTGGATATTGa > 1:2177903/1‑69 (MQ=255) aGGACGCGCGCTGGTGAGTAATCCGCGCGTGCTGTTGCTTGATGAACCGACAAACCACCTGGATATTGa > 1:2422555/1‑69 (MQ=255) aGGACGCGCGCTGGTGAGTAATCCGCGCGTGCTGTTGCTTGATGAACCGACAAACCACCTGGATATTGa > 1:2468840/1‑69 (MQ=255) aGGACGCGCGCTGGTGAGTAATCCGCGCGTGCTGTTGCTTGATGAACCGACAAACCACCTGGATATTGa > 1:2727131/1‑69 (MQ=255) aGGACGCGCGCTGGTGAGTAATCCGCGCGTGCTGTTGCTTGATGAACCGACAAACCACCTGGATATTGa > 1:1337583/1‑69 (MQ=255) aGGACGCGCGCTGGTGAGTAATCCGCGCGTGCTGTTGCTTGATGAACCGACAAACCACCTGGATATTGa > 1:3176883/1‑69 (MQ=255) aGGACGCGCGCTGGTGAGTAATCCGCGCGTGCTGTTGCTTGATGAACCGACAAACCACCTGGATATTGa > 1:479633/1‑69 (MQ=255) cgcgcgCTGGTGAGTAATCCGCGCGTGCTGTTGCTTGATGAACCGACAAACCACCTGGATATTGAAAcc > 1:3154142/1‑69 (MQ=255) gcgcTGGTGAGTAATCCGCGCGTGCTGTTGCTTGATGAACCGACAAACCACCTGGATATTGAAACCATc < 1:1987580/69‑1 (MQ=255) gcgcTGGTGAGTAATCCGCGCGTGCTGTTGCTTGATGAACCGACAAACCACCTGGATATTGAAACCATc < 1:298459/69‑1 (MQ=255) gcgcTGGTGAGTAATCCGCGCGTGCTGTTGCTTGATGAACCGACAAACCACCTGGATATTGAAACCATc < 1:1853036/69‑1 (MQ=255) gcgcTGGTGAGTAATCCGCGCGTGCTGTTGCTTGATGAACCGACAAACCACCTGGATATTGAAACCATc < 1:700485/69‑1 (MQ=255) gcgcTGGTGAGTAATCCGCGCGTGCTGTTGCTTGATGAACCGACAAACCACCTGGATATTGAAACCATc < 1:961651/69‑1 (MQ=255) gcgcTGGTGAGTAATCCGCGCGTGCTGTTGCTTGATGAACCGACAAACCACCTGGATATTGAAACCATc < 1:696481/69‑1 (MQ=255) gcgcTGGTGAGTAATCCGCGCGTGCTGTTGCTTGATGAACCGACAAACCACCTGGATATTGAAACCATc < 1:2728768/69‑1 (MQ=255) gcgcTGGTGAGTAATCCGCGCGTGCTGTTGCTTGATGAACCGACAAACCACCTGGATATTGAAACCATc < 1:2499306/69‑1 (MQ=255) gcTGGTGAGTAATCCGCGCGTGCTGTTGCTTGATGAACCGACAAACCACCTGGATATTGAAACCATCGa < 1:1053652/69‑1 (MQ=255) gcTGGTGAGCAATCCGCGCGTATTGCTGCTCGACGAACCGACTAACCACCTGGATATTGAAACCATCGa > 1:636333/1‑69 (MQ=18) gcTGGTGAGCAATCCGCGCGTATTGCTGCTCGACGAACCGACTAACCACCTGGATATTGAAACCATCGa > 1:2399708/1‑69 (MQ=18) gcTGGTGAGCAATCCGCGCGTATTGCTGCTCGACGAACCGACTAACCACCTGGATATTGAAACCATCGa > 1:2659817/1‑69 (MQ=18) gcTGGTGAGCAATCCGCGCGTATTGCTGCTCGACGAACCGACTAACCACCTGGATATTGAAACCATCGa > 1:563901/1‑69 (MQ=18) gcTGGTGAGCAATCCGCGCGTATTGCTGCTCGACGAACCGACTAACCACCTGGATATTGAAACCATCGa > 1:2220447/1‑69 (MQ=18) gcTGGTGAGCAATCCGCGCGTATTGCTGCTCGACGAACCGACTAACCACCTGGATATTGAAACCATCGa > 1:2935919/1‑69 (MQ=18) gcTGGTGAGCAATCCGCGCGTATTGCTGCTCGACGAACCGACTAACCACCTGGATATTGAAACCATCGa > 1:10772/1‑69 (MQ=18) gcTGGTGAGCAATCCGCGCGTATTGCTGCTCGACGAACCGACTAACCACCTGGATATTGAAACCATCGa > 1:556943/1‑69 (MQ=18) gcTGGTGAGCAATCCGCGCGTATTGCTGCTCGACGAACCGACTAACCACCTGGATATTGAAACCATCGa > 1:420757/1‑69 (MQ=18) gcTGGTGAGCAATCCGCGCGTATTGCTGCTCGACGAACCGACTAACCACCTGGATATTGAAACCATCGa > 1:1814099/1‑69 (MQ=18) gcTGGTGAGCAATCCGCGCGTATTGCTGCTCGACGAACCGACTAACCACCTGGATATTGAAACCATCGa > 1:1697239/1‑69 (MQ=18) gcTGGTGAGCAATCCGCGCGTATTGCTGCTCGACGAACCGACTAACCACCTGGATATTGAAACCATCGa > 1:1207253/1‑69 (MQ=18) gcTGGTGAGCAATCCGCGCGTATTGCTGCTCGACGAACCGACTAACCACCTGGATATTGAAACCATCGa > 1:3232348/1‑69 (MQ=18) gcTGGTGAGCAATCCGCGCGTATTGCTGCTCGACGAACCGACTAACCACCTGGATATTGAAACCATCGa > 1:3380215/1‑69 (MQ=18) tGGTGAGCAATCCGCGCGTATTGCTGCTCGACGAACCGACTAACCACCTGGATATTGAAACCATCGAct < 1:1065511/69‑1 (MQ=18) tGGTGAGCAATCCGCGCGTATTGCTGCTCGACGAACCGACTAACCACCTGGATATTGAAACCATCGAct < 1:923040/69‑1 (MQ=18) tGGTGAGCAATCCGCGCGTATTGCTGCTCGACGAACCGACTAACCACCTGGATATTGAAACCATCGAct < 1:825662/69‑1 (MQ=18) tGGTGAGCAATCCGCGCGTATTGCTGCTCGACGAACCGACTAACCACCTGGATATTGAAACCATCGAct < 1:734046/69‑1 (MQ=18) tGGTGAGCAATCCGCGCGTATTGCTGCTCGACGAACCGACTAACCACCTGGATATTGAAACCATCGAct < 1:3170519/69‑1 (MQ=18) tGGTGAGCAATCCGCGCGTATTGCTGCTCGACGAACCGACTAACCACCTGGATATTGAAACCATCGAct < 1:3018352/69‑1 (MQ=18) tGGTGAGCAATCCGCGCGTATTGCTGCTCGACGAACCGACTAACCACCTGGATATTGAAACCATCGAct < 1:2655021/69‑1 (MQ=18) tGGTGAGCAATCCGCGCGTATTGCTGCTCGACGAACCGACTAACCACCTGGATATTGAAACCATCGAct < 1:1912247/69‑1 (MQ=18) tGAGCAATCCGCGCGTTTTGCTGCTCGACGAACCGACTAACCACCTGGATATTGAAACCATCGAct < 1:2833382/66‑1 (MQ=18) cgcgcgTGCTGTTGCTTGATGAACCGACAAACCACCTGGATATTGAAACCATCGACTGGCTGGAAGGGt > 1:2012608/1‑69 (MQ=255) cgcgcgTGCTGTTGCTTGATGAACCGACAAACCACCTGGATATTGAAACCATCGACTGGCTGGAAGGGt > 1:1469273/1‑69 (MQ=255) cgcgcgTGCTGTTGCTTGATGAACCGACAAACCACCTGGATATTGAAACCATCGACTGGCTGGAAGGGt > 1:965127/1‑69 (MQ=255) gCTGTTGCTTGATGAACCGACAAACCACCTTGATATTGAAACCATCGACTGGCTGGAAGGGTTTTTGaa < 1:477533/69‑1 (MQ=255) gCTGTTGCTTGATGAACCGACAAACCACCTGGATATTGAAACCATCGACTGGCTGGAAGGGTTTTTGaa < 1:2397067/69‑1 (MQ=255) gCTGTTGCTTGATGAACCGACAAACCACCTGGATATTGAAACCATCGACTGGCTGGAAGGGTTTTTGaa < 1:2258060/69‑1 (MQ=255) gCTGTTGCTTGATGAACCGACAAACCACCTGGATATTGAAACCATCGACTGGCTGGAAGGGTTTTTGaa < 1:1858905/69‑1 (MQ=255) gCTGTTGCTTGATGAACCGACAAACCACCTGGATATTGAAACCATCGACTGGCTGGAAGGGTTTTTGaa < 1:1609516/69‑1 (MQ=255) gCTGTTGCTTGATGAACCGACAAACCACCTGGATATTGAAACCAACGACTGGCTGGAAGGGTTTTTGaa < 1:2330886/69‑1 (MQ=255) gCTGTCGCTTGATGAACCGACAAACCACCTGGATATTGAAACCATCGACTGGCTGGAAGGGTTTTTGaa < 1:872517/69‑1 (MQ=255) cTGTTGCTTGATGAACCGACAAACCACCTGGATATTGAAACCATCGACTGGCTGGAAGGGTTTTTGaa < 1:97191/68‑1 (MQ=255) | TAAAGCGGCATTAGGACGCGCGCTGGTGAGTAATCCGCGCGTGCTGTTGCTTGATGAACCGACAAACCACCTGGATATTGAAACCATCGACTGGCTGGAAGGGTTTTTGAA > minE/667151‑667261 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |