| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 667,544 | A→G | 19.6% | E294E (GAA→GAG) | uup → | fused predicted transporter subunits and ATP‑binding components of ABC superfamily |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 667,544 | 0 | A | G | 19.6% | 100.0 / 16.1 | 71 | E294E (GAA→GAG) | uup | fused predicted transporter subunits and ATP‑binding components of ABC superfamily |
| Reads supporting (aligned to +/- strand): ref base A (40/17); new base G (10/4); total (50/21) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.42e-01 | |||||||||||
TCAAAGCACGCCGTACCCGTAATGAAGGCCGCGTACGCGCCCTGAAAGCGATGCGTCGCGAACGTGGTGAACGTCGCGAAGTGATGGGTACCGCAAAGATGCAGGTGGAAGAGGCCAGCCGCTCCG > minE/667483‑667608 | tCAAAGCACGCCGTACCCGTAATGAAGGCCGCGTACGCGCCCTGAAAGCGATGCGTCGCGAACgtggtg < 1:926749/69‑1 (MQ=255) cAAAGCACGCCGTACCCGTAATGAAGGCCGCGTACGCGCCCTGAAAGCGATGCGTCGCGAACGTGGTGa > 1:1041856/1‑69 (MQ=255) cAAAGCACGCCGTACCCGTAATGAAGGCCGCGTACGCGCCCTGAAAGCGATGCGTCGCGAACGTGGTGa > 1:402620/1‑69 (MQ=255) cAAAGCACGCCGTACCCGTAATGAAGGCCGCGTACGCGCCCTGAAAGCGATGCGTCGCGAACGTGGTGa > 1:2561595/1‑69 (MQ=255) cAAAGCACGCCGTACCCGTAATGAAGGCCGCGTACGCGCCCTGAAAGCGATGCGTCGCGAACGTGGTGa > 1:686949/1‑69 (MQ=255) cAAAGCACGCCGTACCCGTAATGAAGGCCGCGTACGCGCCCTGAAAGCGATGCGTCGCGAACGTGGTGa > 1:2541293/1‑69 (MQ=255) cAAAGCACGCCGTACCCGTAATGAAGGCCGCGTACGCGCCCTGAAAGCGATGCGTCGCGAACGTGGTGa > 1:690843/1‑69 (MQ=255) cAAAGCACGCCGTACCCGTAATGAAGGCCGCGTACGCGCCCTGAAAGCGATGCGTCGCGAACGTGGTGa > 1:2309022/1‑69 (MQ=255) cAAAGCACGCCGTACCCGTAATGAAGGCCGCGTACGCGCCCTGAAAGCGATGCGTCGCGAACGTGGTGa > 1:2282935/1‑69 (MQ=255) cAAAGCACGCCGTACCCGTAATGAAGGCCGCGTACGCGCCCTGAAAGCGATGCGTCGCGAACGTGGTGa > 1:795434/1‑69 (MQ=255) cAAAGCACGCCGTACCCGTAATGAAGGCCGCGTACGCGCCCTGAAAGCGATGCGTCGCGAACGTGGTGa > 1:2077407/1‑69 (MQ=255) cAAAGCACGCCGTACCCGTAATGAAGGCCGCGTACGCGCCCTGAAAGCGATGCGTCGCGAACGTGGTGa > 1:807626/1‑69 (MQ=255) cAAAGCACGCCGTACCCGTAATGAAGGCCGCGTACGCGCCCTGAAAGCGATGCGTCGCGAACGTGGTGa > 1:1646150/1‑69 (MQ=255) cAAAGCACGCCGTACCCGTAATGAAGGCCGCGTACGCGCCCTGAAAGCGATGCGTCGCGAACGTGGTGa > 1:13302/1‑69 (MQ=255) cAAAGCACGCCGTACCCGTAATGAAGGCCGCGTACGCGCCCTGAAAGCGATGCGTCGCGAACGTGGTGa > 1:1222548/1‑69 (MQ=255) cAAAGCACGCCGTACCCGTAATGAAGGCCGCGTACGCGCCCTGAAAGCGATGCGTCGCGAACGTGGTGa > 1:1384661/1‑69 (MQ=255) cAAAGCACGCCGTACCCGTAATGAAGGCCACGTACGCGCCCTGAAAGCGATGCGTCGCGAACGTGGTGa > 1:2823918/1‑69 (MQ=255) tACCCGTAATGAAGGCCGCGTACGCGCCCTGAAAGCGATGCGTCGCGAACGTGGTGAACGTCGCGAAGt < 1:2509175/69‑1 (MQ=255) tACCCGTAATGAAGGCCGCGTACGCGCCCTGAAAGCGATGCGTCGCGAACGTGGTGAACGTCGCGAAGt < 1:2741862/69‑1 (MQ=255) tACCCGTAATGAAGGCCGCGTACGCGCCCTGAAAGCGATGCGTCGCGAACGTGGTGAACGTCGCGAAGt < 1:2919364/69‑1 (MQ=255) tACCCGTAATGAAGGCCGCGTACGCGCCCTGAAAGCGATGCGTCGCGAACGTGGTGAACGTCGCGAAGt < 1:2255472/69‑1 (MQ=255) tACCCGTAATGAAGGCCGCGTACGCGCCCTGAAAGCGATGCGTCGCGAACGTGGTGAACGTCGCGAAGt < 1:3109386/69‑1 (MQ=255) tACCCGTAATGAAGGCCGCGTACGCGCCCTGAAAGCGATGCGTCGCGAACGTGGTGAACGTCGCGAAGt < 1:1446903/69‑1 (MQ=255) tACCCGTAATGAAGGCCGCGTACGCGCCCTGAAAGCGATGCGTCGCGAACGTGGTGAACGTCGCGAAGt < 1:3286428/69‑1 (MQ=255) tACCCGTAATGAAGGCCGCGTACGCGCCCTGAAAGCGATGCGTCGCGAACGTGGTGAACGTCGCGAAGt < 1:1591098/69‑1 (MQ=255) aTGAAGTCCGCGTACGCGCCCTGAAAGCGATGCGTCGCGAACGTGGTGAACGTCGCGAAGTGATGGGTa > 1:439082/1‑69 (MQ=255) aTGAAGGCCGCGTACGCGCCCTGAAAGCGATGCGTCGCGAACGTGGTGAACGTCGCGAAGTGATggg > 1:2079105/1‑67 (MQ=255) aTGAAGGCCGCGTACGCGCCCTGAAAGCGATGCGTCGCGAACGTGGTGAACGTCGCGAAGTGATGGGt > 1:3374372/1‑68 (MQ=255) aTGAAGGCCGCGTACGCGCCCTGAAAGCGATGCGTCGCGAACGTGGTGAACGTCGCGAAGTGATGGGTa > 1:2993067/1‑69 (MQ=255) aTGAAGGCCGCGTACGCGCCCTGAAAGCGATGCGTCGCGAACGTGGTGAACGTCGCGAAGTGATGGGTa > 1:605635/1‑69 (MQ=255) aTGAAGGCCGCGTACGCGCCCTGAAAGCGATGCGTCGCGAACGTGGTGAACGTCGCGAAGTGATGGGTa > 1:52771/1‑69 (MQ=255) aTGAAGGCCGCGTACGCGCCCTGAAAGCGATGCGTCGCGAACGTGGTGAACGTCGCGAAGTGATGGGTa > 1:684876/1‑69 (MQ=255) aTGAAGGCCGCGTACGCGCCCTGAAAGCGATGCGTCGCGAACGTGGTGAACGTCGCGAAGTGATGGGTa > 1:552704/1‑69 (MQ=255) aTGAAGGCCGCGTACGCGCCCTGAAAGCGATGCGTCGCGAACGTGGTGAACGTCGCGAAGTGATGGGTa > 1:129462/1‑69 (MQ=255) aTGAAGGCCGCGTACGCGCCCTGAAAGCGATGCGTCGCGAACGTGGTGAACGTCGCGAAGTGATGGGTa > 1:598807/1‑69 (MQ=255) aTGAAGGCCGCGTACGCGCCCTGAAAGCGATGCGTCGCGAACGTGGTGAACGTCGCGAAGTGATGGGTa > 1:1667470/1‑69 (MQ=255) aTGAAGGCCGCGTACGCGCCCTGAAAGCGATGCGTCGCGAACGTGGTGAACGTCGCGAAGTGATGGGTa > 1:1705764/1‑69 (MQ=255) aTGAAGGCCGCGTACGCGCCCTGAAAGCGATGCGTCGCGAACGTGGTGAACGTCGCGAAGTGATGGGTa > 1:830993/1‑69 (MQ=255) aTGAAGGCCGCGTACGCGCCCTGAAAGCGATGCGTCGCGAACGTGGTGAACGTCGCGAAGTGATGGGTa > 1:2335138/1‑69 (MQ=255) aTGAAGGCCGCGTACGCGCCCTGAAAGCGATGCGTCGCGAACGTGGTGAACGTCGCGAAGTGATGGGTa > 1:1340659/1‑69 (MQ=255) aTGAAGGCCGCGTACGCGCCCTGAAAGCGATGCGTCGCGAACGTGGTGAACGTCGCGAAGTGATGGGTa > 1:697870/1‑69 (MQ=255) aTGAAGGCCGCGTACGCGCCCTGAAAGCGATGCGTCGCGAACGTGGTGAACGTCGCGAAGTGATGGGTa > 1:2172075/1‑69 (MQ=255) aTGAAGGCCGCGTACGCGCCCTGAAAGCGATGCGTCGCGAACGTGGTGAACGTCGCGAAGTGATGGGTa > 1:2158944/1‑69 (MQ=255) aTGAAGGCCGCGTACGCGCCCTGAAAGCGATGCGTCGCGAACGTGGTGAACGTCGCGAAGTGATGGGTa > 1:1896074/1‑69 (MQ=255) aTGAAGGCCGCGTACGCGCCCTGAAAGCGATGCGTCGCGAACGTGGTGAACGTCGCGAAGTGATGGGTa > 1:2108316/1‑69 (MQ=255) aTGAAGGCCGCGTACGCGCCCTGAAAGCGATGCGTCGCGAACGTGGTGAACGTCGCGAAGTGATGGGTa > 1:1924461/1‑69 (MQ=255) aTGAAGGCCGCGTACGCGCCCTGAAAGCGATGCGTCGCGAACGTGGTGAACGGCGCGAAGTGATGGGTa > 1:426860/1‑69 (MQ=255) aTGAAGGCCGCGTACGCGCCCTGAAAGCGATGCGTCGCGAACGTGGCGAACGTCGCGAAGTGATGGGTa > 1:1334324/1‑69 (MQ=255) aTGAAGGCCGCGTACGCGCCCTGAAAGCGATGCGTCGCGAACGT‑GTGAACGTCGCGAAGTGATGGGTAc > 1:941090/1‑69 (MQ=255) gCCGCGTACGCGCCCTGAAAGCGATGCGTCGCGAACGTGGTGAACGTCGCGAAGTGATggg < 1:3123684/61‑1 (MQ=255) gCCGCGTACGCGCCCTGAAAGCGATGCGTCGCGAACGTGGTGAACGTCGCGAAGTGATggg < 1:1736848/61‑1 (MQ=255) gCCGCGTACGCGCCCTGAAAGCGATGCGTCGCGAACGTGGTGAACGTCGCGAAGTGATggg < 1:2456115/61‑1 (MQ=255) gCCGCGTACGCGCCCTGAAAGCGATGCGTCGCGAACGTGGTGAACGTCGCGAAGTGATggg < 1:245157/61‑1 (MQ=255) tACGCGCCCTGAAAGCGATGCGTCGCGAACGTGGTGAACGTCGCGAAGTGATggg < 1:2352968/55‑1 (MQ=255) cgcgCCCTGAAAGCGATGCGTCGCGAACGTGGTGAACGTCGCGAAGt < 1:953726/47‑1 (MQ=255) cgcgCCCTGAAAGCGATGCGTCGCGAACGTGGTGAACGTCGCGAAGt < 1:651986/47‑1 (MQ=255) cgcgCCCTGAAAGCGATGCGTCGCGAACGTGGTGAACGTCGCGAAGt < 1:1731530/47‑1 (MQ=255) aGCAATGCGTCGCGAGCGTGGTGAACGTCGCGAAGTAATGGGTACCGCAAAGATGCAGGTgg > 1:2693735/1‑62 (MQ=255) aGCAATGCGTCGCGAGCGTGGTGAACGTCGCGAAGTAATGGGTACCGCAAAGATGCAGGTGGAagaa > 1:3423396/1‑66 (MQ=255) aGCAATGCGTCGCGAGCGTGGTGAACGTCGCGAAGTAATGGGTACCGCAAAGATGCAGGTGGAagaa > 1:606647/1‑66 (MQ=255) aGCAATGCGTCGCGAGCGTGGTGAACGTCGCGAAGTAATGGGTACCGCAAAGATGCAGGTGGAagaa > 1:2554601/1‑66 (MQ=255) aGCAATGCGTCGCGAGCGTGGTGAACGTCGCGAAGTAATGGGTACCGCAAAGATGCAGGTGGAagaa > 1:2363854/1‑66 (MQ=255) aGCAATGCGTCGCGAGCGTGGTGAACGTCGCGAAGTAATGGGTACCGCAAAGATGCAGGTGGAagaa > 1:2264788/1‑66 (MQ=255) aGCAATGCGTCGCGAGCGTGGTGAACGTCGCGAAGTAATGGGTACCGCAAAGATGCAGGTGGAagaa > 1:2151626/1‑66 (MQ=255) aGCAATGCGTCGCGAGCGTGGTGAACGTCGCGAAGTAATGGGTACCGCAAAGATGCAGGTGGAagaa > 1:2046403/1‑66 (MQ=255) aGCAATGCGTCGCGAGCGTGGTGAACGTCGCGAAGTAATGGGTACCGCAAAGATGCAGGTGGAagaa > 1:1596545/1‑66 (MQ=255) aGCAATGCGTCGCGAGCGTGGTGAACGTCGCGAAGTAATGGGTACCGCAAAGATGCAGGTGGAagaa > 1:1155726/1‑66 (MQ=255) gcgAGCGTGGTGAACGTCGCGAAGTAATGGGTACCGCAAAGATGCAGGTGGAAGAAGCCAGCCGCTCCg < 1:593131/69‑1 (MQ=255) gcgAGCGTGGTGAACGTCGCGAAGTAATGGGTACCGCAAAGATGCAGGTGGAAGAAGCCAGCCGCTCCg < 1:2892820/69‑1 (MQ=255) gcgAGCGTGGTGAACGTCGCGAAGTAATGGGTACCGCAAAGATGCAGGTGGAAGAAGCCAGCCGCTCCg < 1:901034/69‑1 (MQ=255) gcgAGCGTGGTGAACGTCGCGAAGTAATGGGTACCGCAAAGATGCAGGTGGAAGAAGCCAGCCGCTCCg < 1:1601589/69‑1 (MQ=255) | TCAAAGCACGCCGTACCCGTAATGAAGGCCGCGTACGCGCCCTGAAAGCGATGCGTCGCGAACGTGGTGAACGTCGCGAAGTGATGGGTACCGCAAAGATGCAGGTGGAAGAGGCCAGCCGCTCCG > minE/667483‑667608 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |