Predicted mutation
evidence seq id position mutation freq annotation gene description
RA minE 667,565 G→A 30.3% V301V (GTG→GTA uup → fused predicted transporter subunits and ATP‑binding components of ABC superfamily

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*minE667,5650GA30.3% 40.8 / 18.6 46V301V (GTG→GTAuupfused predicted transporter subunits and ATP‑binding components of ABC superfamily
Reads supporting (aligned to +/- strand):  ref base G (24/8);  new base A (10/4);  total (34/12)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.81e-01

ATGAAGGCCGCGTACGCGCCCTGAAAGCGATGCGTCGCGAACGTGGTGAACGTCGCGAAGTGATGGGTACCGCAAAGATGCAGGTGGAAGAGGCCAGCCGCTCCGGTAAAATCGTT  >  minE/667504‑667619
                                                             |                                                      
aTGAAGTCCGCGTACGCGCCCTGAAAGCGATGCGTCGCGAACGTGGTGAACGTCGCGAAGTGATGGGTa                                                 >  1:439082/1‑69 (MQ=255)
aTGAAGGCCGCGTACGCGCCCTGAAAGCGATGCGTCGCGAACGTGGTGAACGTCGCGAAGTGATggg                                                   >  1:2079105/1‑67 (MQ=255)
aTGAAGGCCGCGTACGCGCCCTGAAAGCGATGCGTCGCGAACGTGGTGAACGTCGCGAAGTGATGGGt                                                  >  1:3374372/1‑68 (MQ=255)
aTGAAGGCCGCGTACGCGCCCTGAAAGCGATGCGTCGCGAACGTGGTGAACGTCGCGAAGTGATGGGTa                                                 >  1:1924461/1‑69 (MQ=255)
aTGAAGGCCGCGTACGCGCCCTGAAAGCGATGCGTCGCGAACGTGGTGAACGTCGCGAAGTGATGGGTa                                                 >  1:2993067/1‑69 (MQ=255)
aTGAAGGCCGCGTACGCGCCCTGAAAGCGATGCGTCGCGAACGTGGTGAACGTCGCGAAGTGATGGGTa                                                 >  1:2335138/1‑69 (MQ=255)
aTGAAGGCCGCGTACGCGCCCTGAAAGCGATGCGTCGCGAACGTGGTGAACGTCGCGAAGTGATGGGTa                                                 >  1:2172075/1‑69 (MQ=255)
aTGAAGGCCGCGTACGCGCCCTGAAAGCGATGCGTCGCGAACGTGGTGAACGTCGCGAAGTGATGGGTa                                                 >  1:2158944/1‑69 (MQ=255)
aTGAAGGCCGCGTACGCGCCCTGAAAGCGATGCGTCGCGAACGTGGTGAACGTCGCGAAGTGATGGGTa                                                 >  1:52771/1‑69 (MQ=255)
aTGAAGGCCGCGTACGCGCCCTGAAAGCGATGCGTCGCGAACGTGGTGAACGTCGCGAAGTGATGGGTa                                                 >  1:2108316/1‑69 (MQ=255)
aTGAAGGCCGCGTACGCGCCCTGAAAGCGATGCGTCGCGAACGTGGTGAACGTCGCGAAGTGATGGGTa                                                 >  1:552704/1‑69 (MQ=255)
aTGAAGGCCGCGTACGCGCCCTGAAAGCGATGCGTCGCGAACGTGGTGAACGTCGCGAAGTGATGGGTa                                                 >  1:1896074/1‑69 (MQ=255)
aTGAAGGCCGCGTACGCGCCCTGAAAGCGATGCGTCGCGAACGTGGTGAACGTCGCGAAGTGATGGGTa                                                 >  1:598807/1‑69 (MQ=255)
aTGAAGGCCGCGTACGCGCCCTGAAAGCGATGCGTCGCGAACGTGGTGAACGTCGCGAAGTGATGGGTa                                                 >  1:1705764/1‑69 (MQ=255)
aTGAAGGCCGCGTACGCGCCCTGAAAGCGATGCGTCGCGAACGTGGTGAACGTCGCGAAGTGATGGGTa                                                 >  1:1667470/1‑69 (MQ=255)
aTGAAGGCCGCGTACGCGCCCTGAAAGCGATGCGTCGCGAACGTGGTGAACGTCGCGAAGTGATGGGTa                                                 >  1:605635/1‑69 (MQ=255)
aTGAAGGCCGCGTACGCGCCCTGAAAGCGATGCGTCGCGAACGTGGTGAACGTCGCGAAGTGATGGGTa                                                 >  1:684876/1‑69 (MQ=255)
aTGAAGGCCGCGTACGCGCCCTGAAAGCGATGCGTCGCGAACGTGGTGAACGTCGCGAAGTGATGGGTa                                                 >  1:697870/1‑69 (MQ=255)
aTGAAGGCCGCGTACGCGCCCTGAAAGCGATGCGTCGCGAACGTGGTGAACGTCGCGAAGTGATGGGTa                                                 >  1:1340659/1‑69 (MQ=255)
aTGAAGGCCGCGTACGCGCCCTGAAAGCGATGCGTCGCGAACGTGGTGAACGTCGCGAAGTGATGGGTa                                                 >  1:830993/1‑69 (MQ=255)
aTGAAGGCCGCGTACGCGCCCTGAAAGCGATGCGTCGCGAACGTGGTGAACGTCGCGAAGTGATGGGTa                                                 >  1:129462/1‑69 (MQ=255)
aTGAAGGCCGCGTACGCGCCCTGAAAGCGATGCGTCGCGAACGTGGTGAACGGCGCGAAGTGATGGGTa                                                 >  1:426860/1‑69 (MQ=255)
aTGAAGGCCGCGTACGCGCCCTGAAAGCGATGCGTCGCGAACGTGGCGAACGTCGCGAAGTGATGGGTa                                                 >  1:1334324/1‑69 (MQ=255)
aTGAAGGCCGCGTACGCGCCCTGAAAGCGATGCGTCGCGAACGTGTGAACGTCGCGAAGTGATGGGTAc                                                >  1:941090/1‑69 (MQ=255)
      gCCGCGTACGCGCCCTGAAAGCGATGCGTCGCGAACGTGGTGAACGTCGCGAAGTGATggg                                                   <  1:2456115/61‑1 (MQ=255)
      gCCGCGTACGCGCCCTGAAAGCGATGCGTCGCGAACGTGGTGAACGTCGCGAAGTGATggg                                                   <  1:245157/61‑1 (MQ=255)
      gCCGCGTACGCGCCCTGAAAGCGATGCGTCGCGAACGTGGTGAACGTCGCGAAGTGATggg                                                   <  1:3123684/61‑1 (MQ=255)
      gCCGCGTACGCGCCCTGAAAGCGATGCGTCGCGAACGTGGTGAACGTCGCGAAGTGATggg                                                   <  1:1736848/61‑1 (MQ=255)
            tACGCGCCCTGAAAGCGATGCGTCGCGAACGTGGTGAACGTCGCGAAGTGATggg                                                   <  1:2352968/55‑1 (MQ=255)
                         aGCAATGCGTCGCGAGCGTGGTGAACGTCGCGAAGTAATGGGTACCGCAAAGATGCAGGTgg                               >  1:2693735/1‑62 (MQ=255)
                         aGCAATGCGTCGCGAGCGTGGTGAACGTCGCGAAGTAATGGGTACCGCAAAGATGCAGGTGGAagaa                          >  1:1155726/1‑66 (MQ=255)
                         aGCAATGCGTCGCGAGCGTGGTGAACGTCGCGAAGTAATGGGTACCGCAAAGATGCAGGTGGAagaa                          >  1:1596545/1‑66 (MQ=255)
                         aGCAATGCGTCGCGAGCGTGGTGAACGTCGCGAAGTAATGGGTACCGCAAAGATGCAGGTGGAagaa                          >  1:606647/1‑66 (MQ=255)
                         aGCAATGCGTCGCGAGCGTGGTGAACGTCGCGAAGTAATGGGTACCGCAAAGATGCAGGTGGAagaa                          >  1:2046403/1‑66 (MQ=255)
                         aGCAATGCGTCGCGAGCGTGGTGAACGTCGCGAAGTAATGGGTACCGCAAAGATGCAGGTGGAagaa                          >  1:2151626/1‑66 (MQ=255)
                         aGCAATGCGTCGCGAGCGTGGTGAACGTCGCGAAGTAATGGGTACCGCAAAGATGCAGGTGGAagaa                          >  1:2264788/1‑66 (MQ=255)
                         aGCAATGCGTCGCGAGCGTGGTGAACGTCGCGAAGTAATGGGTACCGCAAAGATGCAGGTGGAagaa                          >  1:3423396/1‑66 (MQ=255)
                         aGCAATGCGTCGCGAGCGTGGTGAACGTCGCGAAGTAATGGGTACCGCAAAGATGCAGGTGGAagaa                          >  1:2363854/1‑66 (MQ=255)
                         aGCAATGCGTCGCGAGCGTGGTGAACGTCGCGAAGTAATGGGTACCGCAAAGATGCAGGTGGAagaa                          >  1:2554601/1‑66 (MQ=255)
                                    gcgAGCGTGGTGAACGTCGCGAAGTAATGGGTACCGCAAAGATGCAGGTGGAAGAAGCCAGCCGCTCCg             <  1:593131/69‑1 (MQ=255)
                                    gcgAGCGTGGTGAACGTCGCGAAGTAATGGGTACCGCAAAGATGCAGGTGGAAGAAGCCAGCCGCTCCg             <  1:1601589/69‑1 (MQ=255)
                                    gcgAGCGTGGTGAACGTCGCGAAGTAATGGGTACCGCAAAGATGCAGGTGGAAGAAGCCAGCCGCTCCg             <  1:2892820/69‑1 (MQ=255)
                                    gcgAGCGTGGTGAACGTCGCGAAGTAATGGGTACCGCAAAGATGCAGGTGGAAGAAGCCAGCCGCTCCg             <  1:901034/69‑1 (MQ=255)
                                               gAACGTCGCGAAGTGATGGGTACCGCAAAGATGCAGGTGGAAGAGGCCAGCCGCTCCGGTAAAATCGtt  <  1:2278682/69‑1 (MQ=255)
                                               gAACGTCGCGAAGTGATGGGTACCGCAAAGATGCAGGTGGAAGAGGCCAGCCGCTCCGGTAAAATCGtt  <  1:1407732/69‑1 (MQ=255)
                                               gAACGTCGCGAAGTGATGGGTACCGCAAAGATGCAGGTGGAAGAGGCCAGCCGCTCCGGTAAAATCGtt  <  1:1338391/69‑1 (MQ=255)
                                                             |                                                      
ATGAAGGCCGCGTACGCGCCCTGAAAGCGATGCGTCGCGAACGTGGTGAACGTCGCGAAGTGATGGGTACCGCAAAGATGCAGGTGGAAGAGGCCAGCCGCTCCGGTAAAATCGTT  >  minE/667504‑667619

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: