| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 69,716 | A→G | 33.9% | K203K (AAA→AAG) | leuO → | DNA‑binding transcriptional activator |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 69,716 | 0 | A | G | 33.9% | 35.5 / 26.9 | 53 | K203K (AAA→AAG) | leuO | DNA‑binding transcriptional activator |
| Reads supporting (aligned to +/- strand): ref base A (20/15); new base G (13/5); total (33/20) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 3.75e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
TTAAAGATGAAATGGTGCTGGTAGCCAGCAAAAATCATCCAACAATTAAGGGCCCGTTACTGAAACATGATGTTTATAACGAACAACATGCGGCGGTTTCGCTCGATCGTTTCGCGTCATTTAGTCAACCTT > minE/69652‑69783 | ttAAAGATGAAATGGTGCTGGTAGCCAGCAAAAATCATCCAACAATTAAGGGCCCGTTACTGAAACat < 1:2439819/68‑1 (MQ=255)ttAAAGATGAAATGGTGCTGGTAGCCAGCAAAAATCATCCAACAATTAAGGGCCCGTTACTGAAACat < 1:2364906/68‑1 (MQ=255)ttAAAGATGAAATGGTGCTGGTAGCCAGCAAAAATCATCCAACAATTAAGGGCCCGTTACTGAAACat < 1:2136834/68‑1 (MQ=255)ttAAAGATGAAATGGTGCTGGTAGCCAGCAAAAATCATCCAACAATTAAGGGCCCGTTACTGAAACat < 1:639689/68‑1 (MQ=255) tAAAGATGAAATGGTGCTGGTAGCCAGCAAAAATCATCCAACAATTAAGGGCCCGTTACTGAAACat < 1:2498100/67‑1 (MQ=255) tAAAGATGAAATGGTGCTGGTAGCCAGCAAAAATCATCCAACAATTAAGGGCCCGTTACTGAAACat < 1:2761354/67‑1 (MQ=255) aaGATGAAATGGTGTTGGTAGCCAGCAAAAATCATCCAACAATTAAAGGCCCGTTACTGAAGCatgatg > 1:3009620/1‑69 (MQ=255) aaGATGAAATGGTGTTGGTAGCCAGCAAAAATCATCCAACAATTAAAGGCCCGTTACTGAAGCatgatg > 1:249559/1‑69 (MQ=255) aaGATGAAATGGTGTTGGTAGCCAGCAAAAATCATCCAACAATTAAAGGCCCGTTACTGAAGCatgatg > 1:3161825/1‑69 (MQ=255) aaGATGAAATGGTGTTGGTAGCCAGCAAAAATCATCCAACAATTAAAGGCCCGTTACTGAAGCatgatg > 1:2685336/1‑69 (MQ=255) aaGATGAAATGGTGTTGGTAGCCAGCAAAAATCATCCAACAATTAAAGGCCCGTTACTGAAGCatgatg > 1:2198002/1‑69 (MQ=255) aaGATGAAATGGTGTTGGTAGCCAGCAAAAATCATCCAACAATTAAAGGCCCGTTACTGAAGCatgatg > 1:2167865/1‑69 (MQ=255) aaGATGAAATGGTGTTGGTAGCCAGCAAAAATCATCCAACAATTAAAGGCCCGTTACTGAAGCatgatg > 1:294905/1‑69 (MQ=255) aaGATGAAATGGTGTTGGTAGCCAGCAAAAATCATCCAACAATTAAAGGCCCGTTACTGAAGCatgatg > 1:598044/1‑69 (MQ=255) aaGATGAAATGGTGTTGGTAGCCAGCAAAAATCATCCAACAATTAAAGGCCCGTTACTGAAGCatgatg > 1:1573352/1‑69 (MQ=255) aaGATGAAATGGTGTTGGTAGCCAGCAAAAATCATCCAACAATTAAAGGCCCGTTACTGAAGCatgatg > 1:1562572/1‑69 (MQ=255) aaGATGAAATGGTGTTGGTAGCCAGCAAAAATCATCCAACAATTAAAGGCCCGTTACTGAAGCatgatg > 1:644186/1‑69 (MQ=255) aaGATGAAATGGTGTTGGTAGCCAGCAAAAATCATCCAACAATTAAAGGCCCGTTACTGAAGCatgatg > 1:2821879/1‑69 (MQ=255) aaGATGAAATGGTGTTGGTAGCCAGCAAAAATCATCCAACAATTAA‑AGCCCGTTACTGAAGCatgatg > 1:167655/1‑68 (MQ=255) aGATGAAATGGTGCTGGTAGCCAGCAAAAATCATCCAACAATTAAGGGCCCGTTACTGAAACatgatg < 1:2195905/68‑1 (MQ=255) gATGAAATGGTGCTGGTAGCCAGCAAAAATCATCCAACAATTAAGGGCCCGTTACTGAAACatgatg < 1:3317844/67‑1 (MQ=255) aTGGTGCTGGTAGCCAGCAAAAATCATCCAACAATTAAGGGCCCGTTACTGAAACATGATGTTTATaa > 1:3010403/1‑68 (MQ=255) gTGCTGGTAGCCAGCAAAAATCATCCAACAATTAAGGGCCCGTTACTGAAACATGATGTTTATAACGa > 1:1817104/1‑68 (MQ=255) gTGCTGGTAGCCAGCAAAAATCATCCAACAATTAAGGGCCCGTTACTGAAACATGATGTTTATAACGa > 1:3176358/1‑68 (MQ=255) gTGCTGGTAGCCAGCAAAAATCATCCAACAATTAAGGGCCCGTTACTGAAACATGATGTTTATAACGa > 1:1864339/1‑68 (MQ=255) gTGCTGGTAGCCAGCAAAAATCATCCAACAATTAAGGGCCCGTTACTGAAACATGATGTTTATAACGa > 1:1065920/1‑68 (MQ=255) aaaaaTCATCCAACAATTAAGGGCCCGTTACTGAAACATGATGTTTATAACGAACAACATGCGGCGGtt > 1:2360186/1‑69 (MQ=255) aaaaaTCATCCAACAATTAAGGGCCCGTTACTGAAACATGATGTTTATAACGAACAACATGCGGCGGtt > 1:2581744/1‑69 (MQ=255) aaaaaTCATCCAACAATTAAGGGCCCGTTACTGAAACATGATGTTTATAACGAACAACATGCGGCGGtt > 1:1803788/1‑69 (MQ=255) aaTCATCCAACAATTAAGGGCCCGTTACTGAAACatgatg < 1:2567748/40‑1 (MQ=255) ttAAAGGCCCGTTACTGAAGCATGATGTTTATAACGaac < 1:2645422/39‑1 (MQ=37) ttAAAGGCCCGTTACTGAAGCATGATGTTTATAACGaac < 1:2482094/39‑1 (MQ=37) ttAAAGGCCCGTTACTGAAGCATGATGTTTATAACGaac < 1:1172996/39‑1 (MQ=37) ttAAAGGCCCGTTACTGAAGCATGATGTTTATAACGaac < 1:75247/39‑1 (MQ=37) ttAAAGGCCCGTTACTGAAGCATGATGTTTATAACGaac < 1:2800523/39‑1 (MQ=37) tAAGGGCCCGTTACTGAAACATGATGTTTATAACGAACAACATGCGGCGGTTTCGCTCGATCGTTTcgc > 1:1143710/1‑69 (MQ=255) tACTGAAACATGATGTTTATAACGAACAACATGCGGCGGTTTCGCTCGATCGTTTCGCGTCATTTAGTc < 1:2889279/69‑1 (MQ=255) tACTGAAACATGATGTTTATAACGAACAACATGCGGCGGTTTCGCTCGATCGTTTCGCGTCATTTAGTc < 1:2462796/69‑1 (MQ=255) tACTGAAACATGATGTTTATAACGAACAACATGCGGCGGTTTCGCTCGATCGTTTCGCGTCATTTAGTc < 1:378818/69‑1 (MQ=255) tACTGAAACATGATGTTTATAACGAACAACATGCGGCGGTTTCGCTCGATCGTTTCGCGTCATTTAGTc < 1:733071/69‑1 (MQ=255) tACTGAAACATGATGTTTATAACGAACAACATGCGGCGGTTTCGCTCGATCGTTTCGCGTCATTTAGTc < 1:1119801/69‑1 (MQ=255) aCTGAAACATGATGTTTATAACGAACAACATGCGGCGGTTTCGCTCGATCGTTTCGCGTCATTTAGTc < 1:2772782/68‑1 (MQ=255) gAAACATGATGTTTATAACGAACAACATGCGGCGGTTTCGCTCGATCGTTTCGCGTc > 1:2327710/1‑57 (MQ=255) gAAACATGATGTTTATAACGAACAACATGCGGCGGTTTCGCTCGATCGTTTCGCGTCATTTAGTCAAcc > 1:2991041/1‑69 (MQ=255) gAAACATGATGTTTATAACGAACAACATGCGGCGGTTTCGCTCGATCGTTTCGCGTCATTTAGTCAAcc > 1:584202/1‑69 (MQ=255) gAAACATGATGTTTATAACGAACAACATGCGGCGGTTTCGCTCGATCGTTTCGCGTCATTTAGTCAAcc > 1:779945/1‑69 (MQ=255) gAAACATGATGTTTATAACGAACAACATGCGGCGGTTTCGCTCGATCGTTTCGCGTCATTTAGTCAAcc > 1:2187229/1‑69 (MQ=255) gAAACATGATGTTTATAACGAACAACATGCGGCGGTTTCGCTCGATCGTTTCGCGTCATTTAGTCAAcc > 1:2074097/1‑69 (MQ=255) gAAACATGATGTTTATAACGAACAACATGCGGCGGTTTCGCTCGATCGTTTCGCGTCATTTAGTCAAcc > 1:196680/1‑69 (MQ=255) gAAACATGATGTTTATAACGAACAACATGCGGCGGTTTCGCTCGATCGTTTCGCGTCATTTAGTCAAcc > 1:1357612/1‑69 (MQ=255) gAAACATGATGTTTATAACGAACAACATGCGGCGGTTTCGCTCGATCGTTTCGCGTCATTTAGTCAAcc > 1:1356436/1‑69 (MQ=255) gAAACATGATGTTTATAACGAACAACATGCGGCGGTTTCGCTCGATCGTTTCGCGTCATTTAGTCAAcc > 1:1133975/1‑69 (MQ=255) gAAACATGATGTTTATAACGAACAACATGCGGCGGTTTCGCTCGATCGTTTCGCGTCATTTAGTCAAc > 1:1789297/1‑68 (MQ=255) aaaCATGATGTTTATAACGAACAACATGCGGCGGTTTCGCTCGATCGTTTCGCGTCATTTAGTCAAcc > 1:2196864/1‑68 (MQ=255) aaaCATGATGTTTATAACGAACAACATGCGGCGGTTTCGCTCGATCGTTTCGCGTCATTTAGTCAACCt > 1:2546890/1‑69 (MQ=255) aCATGATGTTTATAACGAACAACATGCGGCGGTTTCGCTCGATCGTTTCGCGTCATTTAGTCAACCtt > 1:274707/1‑68 (MQ=255) aCATGATGTTTATAACGAACAACATGCGGCGGTTTCGCTCGATCGTTTCGCGTCATTTAGTCAACCtt > 1:2647031/1‑68 (MQ=255) aCATGATGTTTATAACGAACAACATGCGGCGGTTTCGCTCGATCGTTTCGCGTCATTTAGTCAACCtt > 1:2596635/1‑68 (MQ=255) aCATGATGTTTATAACGAACAACATGCGGCGGTTTCGCTCGATCGTTTCGCGTCATTTAGTCAACCtt > 1:2488477/1‑68 (MQ=255) aCATGATGTTTATAACGAACAACATGCGGCGGTTTCGCTCGATCGTTTCGCGTCATTTAGTCAACCtt > 1:2135883/1‑68 (MQ=255) aCATGATGTTTATAACGAACAACATGCGGCGGTTTCGCTCGATCGTTTCGCGTCATTTAGTCAACCtt > 1:1547314/1‑68 (MQ=255) | TTAAAGATGAAATGGTGCTGGTAGCCAGCAAAAATCATCCAACAATTAAGGGCCCGTTACTGAAACATGATGTTTATAACGAACAACATGCGGCGGTTTCGCTCGATCGTTTCGCGTCATTTAGTCAACCTT > minE/69652‑69783 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |