| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 667,631 | A→G | 57.9% | E323E (GAA→GAG) | uup → | fused predicted transporter subunits and ATP‑binding components of ABC superfamily |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 667,631 | 0 | A | G | 57.9% | 20.0 / 45.9 | 64 | E323E (GAA→GAG) | uup | fused predicted transporter subunits and ATP‑binding components of ABC superfamily |
| Reads supporting (aligned to +/- strand): ref base A (17/10); new base G (22/15); total (39/25) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 8.01e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.13e-01 | |||||||||||
GGGTACCGCAAAGATGCAGGTGGAAGAGGCCAGCCGCTCCGGTAAAATCGTTTTCGAAATGGAAGACGTTTGCTACCAGGTTAACGGTAAGCAACTGGTGAAAGATTTTTCTGCCCAGGTTCTA > minE/667568‑667691 | gggTACCGCAAAGATGCAGGTGGAAGAGGCCAGCCGCTCCGGTAAAATCGTTTTCGAAATGGAAGACg < 1:1140726/68‑1 (MQ=255)gggTACCGCAAAGATGCAGGTGGAAGAGGCCAGCCGCTCCGGTAAAATCGTTTTCGAAATGGAAGACg < 1:3416683/68‑1 (MQ=255)gggTACCGCAAAGATGCAGGTGGAAGAGGCCAGCCGCTCCGGTAAAATCGTTTTCGAAATGGAAGACg < 1:2611050/68‑1 (MQ=255)gggTACCGCAAAGATGCAGGTGGAAGAGGCCAGCCGCTCCGGTAAAATCGTTTTCGAAATGGAAGACg < 1:2544094/68‑1 (MQ=255)gggTACCGCAAAGATGCAGGTGGAAGAGGCCAGCCGCTCCGGTAAAATCGTTTTCGAAATGGAAGACg < 1:2482101/68‑1 (MQ=255)gggTACCGCAAAGATGCAGGTGGAAGAGGCCAGCCGCTCCGGTAAAATCGTTTTCGAAATGGAAGACg < 1:205043/68‑1 (MQ=255) gCAAAGATGCAGGTGGAAGAGGCCAGCCGCTCCGGTAAAATCGTTTTCGAAATGGAAGACGTTTGCt > 1:425307/1‑67 (MQ=255) aTGCAGGTGGAAGAAGCCAGCCGCTCCGGTAAGATCGTTTTCGAAATGGAGGACGTTTGCTACCAGGtt < 1:2500714/69‑1 (MQ=39) aTGCAGGTGGAAGAAGCCAGCCGCTCCGGTAAGATCGTTTTCGAAATGGAGGACGTTTGCTACCAGGtt < 1:983622/69‑1 (MQ=39) aTGCAGGTGGAAGAAGCCAGCCGCTCCGGTAAGATCGTTTTCGAAATGGAGGACGTTTGCTACCAGGtt < 1:485337/69‑1 (MQ=39) aTGCAGGTGGAAGAAGCCAGCCGCTCCGGTAAGATCGTTTTCGAAATGGAGGACGTTTGCTACCAGGtt < 1:329933/69‑1 (MQ=39) aTGCAGGTGGAAGAAGCCAGCCGCTCCGGTAAGATCGTTTTCGAAATGGAGGACGTTTGCTACCAGGtt < 1:3006710/69‑1 (MQ=39) aTGCAGGTGGAAGAAGCCAGCCGCTCCGGTAAGATCGTTTTCGAAATGGAGGACGTTTGCTACCAGGtt < 1:3006613/69‑1 (MQ=39) aTGCAGGTGGAAGAAGCCAGCCGCTCCGGTAAGATCGTTTTCGAAATGGAGGACGTTTGCTACCAGGtt < 1:2350271/69‑1 (MQ=39) aTGCAGGTGGAAGAAGCCAGCCGCTCCGGTAAGATCGTTTTCGAAATGGAGGACGTTTGCTACCAGGtt < 1:2247999/69‑1 (MQ=39) aTGCAGGTGGAAGAAGCCAGCCGCTCCGGTAAGATCGTTTTCGAAATGGAGGACGTTTGCTACCAGGtt < 1:1791083/69‑1 (MQ=39) aTGCAGGTGGAAGAAGCCAGCCGCTCCGGTAAGATCGTTTTCGAAATGGAGGACGTTTGCTACCAGGtt < 1:1698389/69‑1 (MQ=39) aTGCAGGTGGAAGAAGCCAGCCGCTCCGGTAAGATCGTTTTCGAAATGGAGGACGTTTGCTACCAGGtt < 1:1287706/69‑1 (MQ=39) aTGCAGGTGGAAGAAGCCAGCCGCTCCGGTAAGATCGTTTTCGAAATGGAGGACGTTTGCTACCAGGtt < 1:1566675/69‑1 (MQ=39) aTGCAGGTGGAAGAAGCCAGCCGCTCCGGTAAGATCGTTTTCGAAATGGAGGACGTTTGCTACCAGGtt < 1:1246665/69‑1 (MQ=39) aTGCAGGTGGAAGAAGCCAGCCGCTCCGGTAAGATCGTTTTCGAAATGGAGGACGTTTGCTACCAGGtt < 1:1241428/69‑1 (MQ=39) aTGCAGGTGGAAGAAGCAAGCCGCTCCGGTAAGATCGTTTTCGAAATGGAGGACGTTTGCTACCAGGtt < 1:724670/69‑1 (MQ=38) ggAAGAGGCCAGCCGCTCCGGTAAAATCGTTTTCGAAATGGAAGACGTTTGCTACCAGGTTAACGGTa > 1:1966601/1‑68 (MQ=255) ggAAGAGGCCAGCCGCTCCGGTAAAATCGTTTTCGAAATGGAAGACGTTTGCTACCAGGTTAACGGTa > 1:1784573/1‑68 (MQ=255) agagGCCAGCCGCTCCGGTAAAATCGTTTTCGAAATGGAAGACg < 1:724321/44‑1 (MQ=255) ggCCAGCCGCTCCGGTAAAATCGTTTTCGAAATGGAAGACGTTTGCTa > 1:121774/1‑48 (MQ=255) ggCCAGCCGCTCCGGTAAAATCGTTTTCGAAATGGAAGACGTTTGCTACCAGGTTAACGGTAAGCAACt > 1:1829579/1‑69 (MQ=255) ggCCAGCCGCTCCGGTAAAATCGTTTTCGAAATGGAAGACGTTTGCTACCAGGTTAACGGTAAGCAACt > 1:3266431/1‑69 (MQ=255) ggCCAGCCGCTCCGGTAAAATCGTTTTCGAAAAGGAAGACGTTTGCTAcc > 1:1503716/1‑50 (MQ=255) ccGGTAAAATCGTTTTCGAAATGGAAGACGTTTGCTACCAGGTTAACGGTAAGCa > 1:2925530/1‑55 (MQ=255) ccGGTAAAATCGTTTTCGAAATGGAAGACGTTTGCTACCAGGTTAACGGTAAGCa > 1:798473/1‑55 (MQ=255) ccGGTAAAATCGTTTTCGAAATGGAAGACGTTTGCTACCAGGTTAACGGTAAGCa > 1:3055668/1‑55 (MQ=255) ccGGTAAAATCGTTTTCGAAATGGAAGACGTTTGCTACCAGGTTAACGGTAAGCa > 1:804994/1‑55 (MQ=255) ccGGTAAAATCGTTTTCGAAATGGAAGACGTTTGCTACCAGGTTAACGGTAAGCa > 1:1938419/1‑55 (MQ=255) ccGGTAAAATCGTTTTCGAAATGGAAGACGTTTGCTACCAGGTTAACGGTAAGCa > 1:2484191/1‑55 (MQ=255) aTCGTTTTCGAAATGGAAGACGTTTGCTACCAGGTTAACGGTAAGCAAc > 1:1935795/1‑49 (MQ=255) aTCGTTTTCGAAATGGAAGACGTTTGCTACCAGGTTAACGGTAAGCAACTGGTGAAAGATTTTTCTg > 1:1118874/1‑67 (MQ=255) aTCGTTTTCGAAATGGAAGACGTTTGCTACCAGGTTAACGGTAAGCAACTGGTGAAAGATTTTTCTg > 1:891119/1‑67 (MQ=255) cGTTTTCGAAATGGAAGACGTTTGCTACCAGGTTAACGGTAAGCAACTGGTGAAAGATTTTTCTGCCCa > 1:1298011/1‑69 (MQ=255) gTTTTCGAAATGGAAGACGTTTGCTACCAGGTTAACGGTAGGCAACTGGTGAAAGATTTTTCTGCCCAg < 1:188906/69‑1 (MQ=255) gTTTTCGAAATGGAAGACGTTTGCTACCAGGTTAACGGTAAGCAACTGGTGAAAGATTTTTCTGCCCAg < 1:3273113/69‑1 (MQ=255) ttttCGAAATGGAAGACGTTTGCTACCAGGTTAACGGTAAGCAACTGGTGAAAGATTTTTCTGCCCAg < 1:1053764/68‑1 (MQ=255) aaaggaggaCGTTTGCTACCAGGTTGACGGTAAGCAACTGGTGAAAGATTTTTCTGCCCAGGTTCTa > 1:429647/4‑67 (MQ=255) aaTGGAGGACGTTTGCTACCAGGTTGACGGTAAGCAACTGGTGAAAGATTTTTCTGccct > 1:807019/1‑59 (MQ=255) aaTGGAGGACGTTTGCTACCAGGTTGACGGTAAGCAACTGGTGAAAGATTTTTCTGCCCAGGTTCt > 1:2224198/1‑66 (MQ=255) aaTGGAGGACGTTTGCTACCAGGTTGACGGTAAGCAACTGGTGAAAGATTTTTCTGCCCAGGTTCTa > 1:1375074/1‑67 (MQ=255) aaTGGAGGACGTTTGCTACCAGGTTGACGGTAAGCAACTGGTGAAAGATTTTTCTGCCCAGGTTCTa > 1:2714788/1‑67 (MQ=255) aaTGGAGGACGTTTGCTACCAGGTTGACGGTAAGCAACTGGTGAAAGATTTTTCTGCCCAGGTTCTa > 1:262377/1‑67 (MQ=255) aaTGGAGGACGTTTGCTACCAGGTTGACGGTAAGCAACTGGTGAAAGATTTTTCTGCCCAGGTTCTa > 1:820423/1‑67 (MQ=255) aaTGGAGGACGTTTGCTACCAGGTTGACGGTAAGCAACTGGTGAAAGATTTTTCTGCCCAGGTTCTa > 1:1591768/1‑67 (MQ=255) aaTGGAGGACGTTTGCTACCAGGTTGACGGTAAGCAACTGGTGAAAGATTTTTCTGCCCAGGTTCTa > 1:762017/1‑67 (MQ=255) aaTGGAGGACGTTTGCTACCAGGTTGACGGTAAGCAACTGGTGAAAGATTTTTCTGCCCAGGTTCTa > 1:754672/1‑67 (MQ=255) aaTGGAGGACGTTTGCTACCAGGTTGACGGTAAGCAACTGGTGAAAGATTTTTCTGCCCAGGTTCTa > 1:1895672/1‑67 (MQ=255) aaTGGAGGACGTTTGCTACCAGGTTGACGGTAAGCAACTGGTGAAAGATTTTTCTGCCCAGGTTCTa > 1:2657512/1‑67 (MQ=255) aaTGGAGGACGTTTGCTACCAGGTTGACGGTAAGCAACTGGTGAAAGATTTTTCTGCCCAGGTTCTa > 1:553308/1‑67 (MQ=255) aaTGGAGGACGTTTGCTACCAGGTTGACGGTAAGCAACTGGTGAAAGATTTTTCTGCCCAGGTTCTa > 1:500713/1‑67 (MQ=255) aaTGGAGGACGTTTGCTACCAGGTTGACGGTAAGCAACTGGTGAAAGATTTTTCTGCCCAGGTTCTa > 1:1179233/1‑67 (MQ=255) aaTGGAGGACGTTTGCTACCAGGTTGACGGTAAGCAACTGGTGAAAGATTTTTCTGCCCAGGTTCTa > 1:2679999/1‑67 (MQ=255) aaTGGAGGACGTTTGCTACCAGGTTGACGGTAAGCAACTGGTGAAAGATTTTTCTGCCCAGGTTCTa > 1:412276/1‑67 (MQ=255) aaTGGAGGACGTTTGCTACCAGGTTGACGGTAAGCAACTGGTGAAAGATTTTTCTGCCCAGGTTCTa > 1:1579788/1‑67 (MQ=255) aaTGGAGGACGTTTGCTACCAGGTTGACGGTAAGCAACTGGTGAAAGATTTTTCTGCCCAGGTTCTa > 1:1453056/1‑67 (MQ=255) aaTGGAGGACGTTTGCTACCAGGTTGACGGTAAGCAACTGGTGAAAGATTTTTCTGCCCAGGTTCTa > 1:1264616/1‑67 (MQ=255) aaTGGAGGACGTTTGCTACCAGGTTGACGGTAAGCAACTGGTGAAAGATTTTTCTGCCCAGGTTCTa > 1:2705660/1‑67 (MQ=255) aaTGGAGGACGTTTGCTACCAGGTTGACGGTAAGCAACTGGTGAAAGATTTTTCTGCCCAGGTTCTa > 1:2993379/1‑67 (MQ=255) aaTAGAGGACGTTTGCTACCAGGTTGACGGTAAGCAACTGGTGAAAGATTTTTCTGCCCAGGTTCTa > 1:2412452/1‑67 (MQ=255) | GGGTACCGCAAAGATGCAGGTGGAAGAGGCCAGCCGCTCCGGTAAAATCGTTTTCGAAATGGAAGACGTTTGCTACCAGGTTAACGGTAAGCAACTGGTGAAAGATTTTTCTGCCCAGGTTCTA > minE/667568‑667691 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |