| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 668,000 | G→T | 45.3% | R446R (CGG→CGT) | uup → | fused predicted transporter subunits and ATP‑binding components of ABC superfamily |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 668,000 | 0 | G | T | 45.3% | 45.7 / 120.1 | 117 | R446R (CGG→CGT) | uup | fused predicted transporter subunits and ATP‑binding components of ABC superfamily |
| Reads supporting (aligned to +/- strand): ref base G (51/13); new base T (38/15); total (89/28) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 3.85e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.91e-01 | |||||||||||
Only 100 of 117 total aligned reads displayed.
TGCAGGACTTTCTGTTCCATCCGAAACGGGCGATGACGCCGGTACGTGCGCTTTCTGGCGGTGAGCGGAACCGCTTGCTGCTGGCGCGTTTGTTCCTCAAACCAAGCAACTTATTGATTCTTGACGAACCG > minE/667933‑668063 | tGCAGGACTTTCTGTTCCATCCGAAACGGGCGATGACGCCGGTACGTGCGCTTTCTGGCGGTGAGCGGa > 1:1793046/1‑69 (MQ=255) aCTTTCTGTTCCATCCGAAACGGGCGATGACGCCGGTACGTGCGCTTTCTGGCGGTGAGCGGAACCGCt > 1:1786705/1‑69 (MQ=255) aCTTTCTGTTCCATCCGAAACGGGCGATGACGCCGGTACGTGCGCTTTCTGGCGGTGAGCGGAACCGCt > 1:2367585/1‑69 (MQ=255) aCTTTCTGTTCCATCCGAAACGGGCGATGACGCCGGTACGTGCGCTTTCTGGCGGTGAGCGGAACCGCt > 1:1888238/1‑69 (MQ=255) aCTTTCTGTTCCATCCGAAACGGGCGATGACGCCGGTACGTGCGCTTTCTGGCGGTGAGCGGAACCGCt > 1:2781997/1‑69 (MQ=255) aCTTTCTGTTCCATCCGAAACGGGCGATGACGCCGGTACGTGCGCTTTCTGGCGGTGAGCGGAACCGCt > 1:2820829/1‑69 (MQ=255) aCTTTCTGTTCCATCCGAAACGGGCGATGACGCCGGTACGTGCGCTTTCTGGCGGTGAGCGGAACCGCt > 1:2917518/1‑69 (MQ=255) cTTTCTGTTCCATCCGAAACGGGCGATGACGCCGGTACGTGCGCTTTCTGGCGGTGAGCGGAACCGCtt > 1:344402/1‑69 (MQ=255) cTTTCTGTTCCATCCGAAACGGGCGATGACGCCGGTACGTGCGCTTTCTGGCGGTGAGCGGAACCGCtt > 1:743090/1‑69 (MQ=255) cTTTCTGTTCCATCCGAAACGGGCGATGACGCCGGTACGTGCGCTTTCTGGCGGTGAGCGGAACCGCtt > 1:1733344/1‑69 (MQ=255) cTTTCTGTTCCATCCGAAACGGGCGATGACGCCGGTACGTGCGCTTTCTGGCGGTGAGCGGAACCGCtt > 1:2782550/1‑69 (MQ=255) ttccTGTTCCATCCAAAACGGGCGATGACGCCGGTACGTGCGCTTTCTGGCGGTGAGCGTAACCGCtt > 1:3237304/4‑68 (MQ=255) ttccTGTTCCATCCAAAACGGGCGATGACGCCGGTACGTGCGCTTTCTGGCGGTGAGCGTAACCGCTtg > 1:3030623/4‑69 (MQ=255) ttccTGTTCCATCCAAAACGGGCGATGACGCCGGTACGTGCGCTTTCTGGCGGTGAGCGTAACCGCTtg > 1:3061074/4‑69 (MQ=255) ttccTGTTCCATCCAAAACGGGCGATGACGCCGGTACGTGCGCTTTCTGGCGGTGAGCGTAACCGCTtg > 1:2474632/4‑69 (MQ=255) ttccTGTTCCATCCAAAACGGGCGATGACGCCGGTACGTGCGCTTTCTGGCGGTGAGCGTAACCGCTtg > 1:3333470/4‑69 (MQ=255) ttccTGTTCCATCCAAAACGGGCGATGACGCCGGTACGTGCGCTTTCTGGCGGTGAGCGTAACCGCTtg > 1:2023033/4‑69 (MQ=255) ttccTGTTCCATCCAAAACGGGCGATGACGCCGGTACGTGCGCTTTCTGGCGGTGAGCGTAACCGCTtg > 1:2647636/4‑69 (MQ=255) ttccTGTTCCATCCAAAACGGGCGATGACGCCGGTACGTGCGCTTTCTGGCGGTGAGCGTAACCGCTtg > 1:1794794/4‑69 (MQ=255) ttccTGTTCCATCCAAAACGGGCGATGACGCCGGTACGTGCGCTTTCTGGCGGTGAGCGTAACCGCTtg > 1:1332244/4‑69 (MQ=255) ttccTGTTCCATCCAAAACGGGCGATGACGCCGGTACGTGCGCTTTCTGGCGGTGAGCGTAACCGCTtg > 1:2858303/4‑69 (MQ=255) ttccTGTTCCATCCAAAACGGGCGATGACGCCGGTACGTGCGCTTTCTGGCGGTGAGCGTAACCGCTtg > 1:2785749/4‑69 (MQ=255) ttccTGTTCCATCCAAAACGGGCGATGACACCGGTACGTGCGCTTTCTGGCGGTGAGCGTAACCGCTtg > 1:3149658/4‑69 (MQ=255) cGAAACGGGCGATGACGCCGGTACGTGCGCTTTCTGGCGGTGAGCGGAACCGCTTGCTGCTGGCGCGtt > 1:908714/1‑69 (MQ=255) cGAAACGGGCGATGACGCCGGTACGTGCGCTTTCTGGCGGTGAGCGGAACCGCTTGCTGCTGGCGCGtt > 1:942505/1‑69 (MQ=255) cGAAACGGGCGATGACGCCGGTACGTGCGCTTTCTGGCGGTGAGCGGAACCGCTTGCTGCTGGCGCGtt > 1:2901904/1‑69 (MQ=255) cGAAACGGGCGATGACGCCGGTACGTGCGCTTTCTGGCGGTGAGCGGAACCGCTTGCTGCTGGCGCGtt > 1:3374292/1‑69 (MQ=255) cGAAACGGGCGATGACGCCGGTACGTGCGCTTTCTGGCGGTGAGCGGAACCGCTTGCTGCTGGCGCGtt > 1:596916/1‑69 (MQ=255) cGAAACGGGCGATGACGCCGGTACGTGCGCTTTCTGGCGGTGAGCGGAACCGCTTGCTGCTGGCGCGtt > 1:2068619/1‑69 (MQ=255) cGAAACGGGCGATGACGCCGGTACGTGCGCTTTCTGGCGGTGAGCGGAACCGCTTGCTGCTGGCGCGtt > 1:2011128/1‑69 (MQ=255) cGAAACGGGCGATGACGCCGGTACGTGCGCTTTCTGGCGGTGAGCGGAACCGCTTGCTGCTGGCGCGtt > 1:1640776/1‑69 (MQ=255) cGAAACGGGCGATGACGCCGGTACGTGCGCTTTCTGGCGGTGAGCGGAACCGCTTGCTGCTGGCGCGtt > 1:2779448/1‑69 (MQ=255) cGAAACGGGCGATGACGCCGGTACGTGCGCTTTCTGGCGGTGAGCGGAACCGCTTGCTGCTGGCGCGtt > 1:1905899/1‑69 (MQ=255) cGAAACGGGCGATGACGCCGGTACGTGCGCTTTCTGGCGGTGAGCGGAACCGCTTGCTGCTGGCGCGtt > 1:1076759/1‑69 (MQ=255) cGAAACGGGCGATGACGCCGGTACGTGCGCTTTCTGGCGGTGAGCGGAACCGCTTGCTGCTGGCGCGtt > 1:1884976/1‑69 (MQ=255) cGAAACGGGCGATGACGCCGGTACGTGCGCTTTCTGGCGGTGAGCGGAACCGCTTGCTGCTGGCGCGtt > 1:946440/1‑69 (MQ=255) cGAAACGGGCGATGACGCCGGTACGTGCGCTTTCTGGCGGTGAGCGGAACCGCTTGCTGCTGGCGCGtt > 1:809668/1‑69 (MQ=255) cGAAACGGGCGATGACGCCGGTACGTGCGCTTTCTGGCGGTGAGCGGAACCGCTTGCTGCTGGCGCGtt > 1:3228885/1‑69 (MQ=255) cGAAACGGGCGATGACGCCGGTACGTGCGCTTTCTGGCGGTGAGCGGAACCGCTTGCTGCTGGCGCGtt > 1:3111976/1‑69 (MQ=255) cGAAACGGGCGATGACGCCGGTACGTGCGCTTTCTGGCGGTGAGCGGAACCGCTTGCTGCTGGCGCGt > 1:2633666/1‑68 (MQ=255) cGAAACGGGCGATGACGCCGGTACGTGCGCTTTCTGGCGGTGAGCGGAACCGCTTGCTGCTGGCGCGt > 1:1069390/1‑68 (MQ=255) cGAAACGGGCGATGACGCCGGTACGTGCGCTTTCTGGCGGTGAGCGGAACCGCTTGCTGCTGGCGCGt > 1:736808/1‑68 (MQ=255) aaaCGGGCGATGACGCCGGTACGTGCGCTTTCTGGCGGTGAGCGGAACCGCTTGCTGCTGGCGCGTTTg < 1:1668388/69‑1 (MQ=255) aaaCGGGCGATGACGCCGGTACGTGCGCTTTCTGGCGGTGAGCGGAACCGCTTGCTGCTGGCGCGTTTg < 1:398195/69‑1 (MQ=255) aaaCGGGCGATGACGCCGGTACGTGCGCTTTCTGGCGGTGAGCGGAACCGCTTGCTGCTGGCGCGTTTg < 1:1383038/69‑1 (MQ=255) aaaCGGGCGATGACGCCGGTACGTGCGCTTTCTGGCGGTGAGCGGAACCGCTTGCTGCTGGCGCGTTTg < 1:1979783/69‑1 (MQ=255) aaaCGGGCGATGACGCCGGTACGTGCGCTTTCTGGCGGTGAGCGGAACCGCTTGCTGCTGGCGCGTTTg < 1:2268074/69‑1 (MQ=255) aCGGGCGATGACGCCGGTACGTGCGCTTTCTGGCGGTGAGCGGAACCGCTTGCTGCTGGCGCGTTTg < 1:833772/67‑1 (MQ=255) gACGCCGGTACGTGCGCTTTCTGGCGGTGAGCGGAACCGCTTGCTGCTGGCGCGTTTGTTCCTCaaa > 1:91413/1‑67 (MQ=255) aCGCCGGTACGTGCGCTTTCTGGCGGTGAGCGGAACCGCTTGCTGCTGGCGCGTTTGTTCCTCAAACCa > 1:1304048/1‑69 (MQ=255) aCGCCGGTACGTGCGCTTTCTGGCGGTGAGCGGAACCGCTTGCTGCTGGCGCGTTTGTTCCTCAAACCa > 1:532525/1‑69 (MQ=255) ggTACGTGCGCTTTCTTGCGGTGAGCGGAACCGCTTGCTGCTGGCGCGTTTGTTCCTCAAACCAAGCaa < 1:2746629/69‑1 (MQ=255) ggTACGTGCGCTTTCTGGCGGTGAGCGGAACCGCTTGCTGCTGGCGCGTTTGTTCCTCAAACCAAGCaa < 1:58814/69‑1 (MQ=255) ggTACGTGCGCTTTCTGGCGGTGAGCGGAACCGCTTGCTGCTGGCGCGTTTGTTCCTCAAACCAAGCaa < 1:396413/69‑1 (MQ=255) ggTACGTGCGCTTTCTGGCGGTGAGCGGAACCGCTTGCTGCTGGCGCGTTTGTTCCTCAAACCAAGCaa < 1:1810360/69‑1 (MQ=255) ggTACGTGCGCTTTCTGGCGGTGAGCGGAACCGCTTGCTGCTGGCGCGTTTGTTCCTCAAACCAAGCaa < 1:1770488/69‑1 (MQ=255) ggTACGTGCGCTTTCTGGCGGTGAGAGGAACCGCTTGCTGCTGGCGCGTTTGTTCCTCAAACCAAGCaa < 1:2975916/69‑1 (MQ=255) tACGTGCGCTTTCTGGCGGTGAGCGTAACCGCTTGTTGTTGGCGCGTTTGTTCCTCAAACCAAGCAACt < 1:3134252/69‑1 (MQ=255) tACGTGCGCTTTCTGGCGGTGAGCGTAACCGCTTGTTGTTGGCGCGTTTGTTCCTCAAACCAAGCAACt < 1:3030787/69‑1 (MQ=255) tACGTGCGCTTTCTGGCGGTGAGCGTAACCGCTTGTTGTTGGCGCGTTTGTTCCTCAAACCAAGCAACt < 1:1779894/69‑1 (MQ=255) tACGTGCGCTTTCTGGCGGTGAGCGTAACCGCTTGTTGTTGGCGCGTTTGTTCCTCAAACCAAGCAACt < 1:1569890/69‑1 (MQ=255) tACGTGCGCTTTCTGGCGGTGAGCGTAACCGCTTGTTGTTGGCGCGTTTGTTCCTCAAACCAAGCAACt < 1:60811/69‑1 (MQ=255) tACGTGCGCTTTCTGGCGGTGAGCGTAACCGCTTGTTGTTGGCGCGTTTGTTCCTCAAACCAAGCAACt < 1:2667695/69‑1 (MQ=255) tACGTGCGCTTTCTGGCGGTGAGCGTAACCGCTTGTTGTTGGCGCGTTTGTTCCTCAAACCAAGCAACt < 1:215323/69‑1 (MQ=255) tACGTGCGCTTTCTGGCGGTGAGCGTAACCGCTTGTTGTTGGCGCGTTTGTTCCTCAAACCAAGCAACt < 1:2562558/69‑1 (MQ=255) tACGTGCGCTTTCTGGCGGTGAGCGTAACCGCTTGTTGTTGGCGCGTTTGTTCCTCAAACCAAGCAACt < 1:2477488/69‑1 (MQ=255) aCGTGCGCTTTCTGGCGGTGAGCGTAACCGCTTGTTGTTGGCGCGTTTGTTCCTCAAACCAAGCAACt < 1:2907302/68‑1 (MQ=255) aCGTGCGCTTTCTGGCGGTGAGCGTAACCGCTTGTTGTTGGCGCGTTTGTTCCTCAAACCAAGCAACt < 1:2090449/68‑1 (MQ=255) aCGTGCGCTTTCTGGCGGTGAGCGGAACCGCTTGCTGCTGGCGCGTTTGTTCCTCAAACCAAGCAACtg > 1:1127809/1‑68 (MQ=255) tGCGCTTTCTGGCGGTGAGCGGAACCGCTTGCTGCTGGCGCGttt > 1:1125115/1‑45 (MQ=255) tGCGCTTTCTGGCGGTGAGCGGAACCGCTTGCTGCTGGCGCGTTTGTTCCTCAAACCAAGCAACTTAtt > 1:968946/1‑69 (MQ=255) tGCGCTTTCTGGCGGTGAGCGGAACCGCTTGCTGCTGGCGCGTTTGTTCCTCAAACCAAGCAACTTAtt > 1:279897/1‑69 (MQ=255) tGAGCGTAACCGCTTGTTGTTGGGGCGTTTGTTCCTCAAagcaagca > 1:2300490/1‑47 (MQ=255) tGAGCGTAACCGCTTGTTGTTGGCGCGTTTGTTCCTCAAACCaa > 1:2834314/1‑44 (MQ=255) tGAGCGTAACCGCTTGTTGTTGGCGCGTTTGTTCCTCAAACCAAGCa > 1:3127787/1‑47 (MQ=255) tGAGCGTAACCGCTTGTTGTTGGCGCGTTTGTTCCTCAAACCAAGCa > 1:1145694/1‑47 (MQ=255) tGAGCGTAACCGCTTGTTGTTGGCGCGTTTGTTCCTCAAACCAAGCa > 1:1345140/1‑47 (MQ=255) tGAGCGTAACCGCTTGTTGTTGGCGCGTTTGTTCCTCAAACCAAGCa > 1:1347452/1‑47 (MQ=255) tGAGCGTAACCGCTTGTTGTTGGCGCGTTTGTTCCTCAAACCAAGCa > 1:1609611/1‑47 (MQ=255) tGAGCGTAACCGCTTGTTGTTGGCGCGTTTGTTCCTCAAACCAAGCa > 1:1661749/1‑47 (MQ=255) tGAGCGTAACCGCTTGTTGTTGGCGCGTTTGTTCCTCAAACCAAGCa > 1:78699/1‑47 (MQ=255) tGAGCGTAACCGCTTGTTGTTGGCGCGTTTGTTCCTCAAACCAAGCa > 1:762362/1‑47 (MQ=255) tGAGCGTAACCGCTTGTTGTTGGCGCGTTTGTTCCTCAAACCAAGCa > 1:1920898/1‑47 (MQ=255) tGAGCGTAACCGCTTGTTGTTGGCGCGTTTGTTCCTCAAACCAAGCa > 1:643336/1‑47 (MQ=255) tGAGCGTAACCGCTTGTTGTTGGCGCGTTTGTTCCTCAAACCAAGCa > 1:1941652/1‑47 (MQ=255) tGAGCGTAACCGCTTGTTGTTGGCGCGTTTGTTCCTCAAACCAAGCa > 1:2220270/1‑47 (MQ=255) tGAGCGTAACCGCTTGTTGTTGGCGCGTTTGTTCCTCAAACCAAGCa > 1:2566182/1‑47 (MQ=255) tGAGCGTAACCGCTTGTTGTTGGCGCGTTTGTTCCTCAAACCAAGCa > 1:2632846/1‑47 (MQ=255) tGAGCGTAACCGCTTGTTGTTGGCGCGTTTGTTCCTCAAACCAAGCa > 1:3322267/1‑47 (MQ=255) tGAGCGTAACCGCTTGTTGTTGGCGCGTTTGTTCCTCAAACCAAGCa > 1:2675202/1‑47 (MQ=255) tGAGCGTAACCGCTTGTTGTTGGCGCGTTTGTTCCTCAAACCAAGCa > 1:2911466/1‑47 (MQ=255) tGAGCGTAACCGCTTGTTGTTGGCGCGTTTGTTCCTCAAACCAAGCa > 1:3009901/1‑47 (MQ=255) aGCGGAACCGCTTGCTGCTGGCGCGTTTGTTCCTCAAACCAAGCAACTTATTGATTCTTGACGAACCg > 1:2336/1‑68 (MQ=255) aGCGGAACCGCTTGCTGCTGGCGCGTTTGTTCCTCAAACCAAGCAACTTATTGATTCTTGACGAACCg > 1:517180/1‑68 (MQ=255) aGCGGAACCGCTTGCTGCTGGCGCGTTTGTTCCTCAAACCAAGCAACTTATTGATTCTTGACGAACCg > 1:2363265/1‑68 (MQ=255) aGCGGAACCGCTTGCTGCTGGCGCGTTTGTTCCTCAAACCAAGCAACTTATTGATTCTTGACGAACCg > 1:2496825/1‑68 (MQ=255) aGCGGAACCGCTTGCTGCTGGCGCGTTTGTTCCTCAAACCAAGCAACTTATTGATTCTTGACGAACCg > 1:1865120/1‑68 (MQ=255) aGCGGAACCGCTTGCTGCTGGCGCGTTTGTTCCTCAAACCAAGCAACTTATTGATTCTTGACGAACCg > 1:2500842/1‑68 (MQ=255) aGCGGAACCGCTTGCTGCTGGCGCGTTTGTTCCTCAAACCAAGCAACTTATTGATTCTTGACGAACCg > 1:1647923/1‑68 (MQ=255) aGCGGAACCGCTTGCTGCTGGCGCGTTTGTTCCTCAAACCAAGCAACTTATTGATTCTTGACGAACCg > 1:1073460/1‑68 (MQ=255) | TGCAGGACTTTCTGTTCCATCCGAAACGGGCGATGACGCCGGTACGTGCGCTTTCTGGCGGTGAGCGGAACCGCTTGCTGCTGGCGCGTTTGTTCCTCAAACCAAGCAACTTATTGATTCTTGACGAACCG > minE/667933‑668063 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |