| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 669,698 | T→C | 31.2% | H333H (CAT→CAC) | pqiA → | paraquat‑inducible membrane protein A |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 669,698 | 0 | T | C | 31.2% | 85.5 / 49.4 | 99 | H333H (CAT→CAC) | pqiA | paraquat‑inducible membrane protein A |
| Reads supporting (aligned to +/- strand): ref base T (20/48); new base C (24/7); total (44/55) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 9.97e-06 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.91e-01 | |||||||||||
Only 100 of 108 total aligned reads displayed.
CGTTAAAGATGATCGCCATCGCGTGGCTGTGTTGGGATGCCAAAGGGCATGGCAAGCGCGACAGTGAAAGAATGCATTTGATTTATGAAGTTGTTGAGTTTGTAGGCCGCTGGTCGAT > minE/669649‑669766 | cGTTAAAGATGATCGCCATCGCGTGGCTGTGTTGGGATGCCAAAGGGCATGGCAAGCGCGACAGTGaa > 1:299452/1‑68 (MQ=255) gtcaaaGATGATCGCCATCGCGTGGCTGTGTTGGGATGCCAAAGGGCATGGCAAGCGCGACAGTGAAAg < 1:1153909/66‑1 (MQ=255) gTTAAAGATGATCGCCATCGCGTGGCTGTGTTGGTATGCCAAAGGGCATGGCAAGCGCGACAGTGAAAg < 1:1759031/69‑1 (MQ=255) gTTAAAGATGATCGCCATCGCGTGGCTGTGTTGGGATGCCAAAGGGCATGGCAAGCGCGACAGTGAAAg < 1:1685469/69‑1 (MQ=255) gTTAAAGATGATCGCCATCGCGTGGCTGTGTTGGGATGCCAAAGGGCATGGCAAGCGCGACAGTGAAAg < 1:2688021/69‑1 (MQ=255) gTTAAAGATGATCGCCATCGCGTGGCTGTGTTGGGATGCCAAAGGGCATGGCAAGCGCGACAGTGAAAg < 1:2648239/69‑1 (MQ=255) gTTAAAGATGATCGCCATCGCGTGGCTGTGTTGGGATGCCAAAGGGCATGGCAAGCGCGACAGTGAAAg < 1:907021/69‑1 (MQ=255) gTTAAAGATGATCGCCATCGCGTGGCTGTGTTGGGATGCCAAAGGGCATGGCAAGCGCGACAGTGAAAg < 1:801203/69‑1 (MQ=255) gTTAAAGATGATCGCCATCGCGTGGCTGTGTTGGGATGCCAAAGGGCATGGCAAGCGCGACAGTGAAAg < 1:1020597/69‑1 (MQ=255) gTTAAAGATGATCGCCATCGCGTGGCTGTGTTGGGATGCCAAAGGGCATGGCAAGCGCGACAGTGAAAg < 1:484482/69‑1 (MQ=255) gTTAAAGATGATCGCCATCGCGTGGCTGTGTTGGGATGCCAAAGGGCATGGCAAGCGCGACAGTGAAAg < 1:2145731/69‑1 (MQ=255) gTTAAAGATGATCGCCATCGCGTGGCTGTGTTGGGATGCCAAAGGGCATGGCAAGCGCGACAGTGAAAg < 1:729277/69‑1 (MQ=255) gTTAAAGATGATCGCCATCGCGTGGCTGTGTTGGGATGCCAAAGGGCATGGCAAGCGCGACAGTGAAAg < 1:675905/69‑1 (MQ=255) gTTAAAGATGATCGCCATCGCGTGGCGGTGTTGGGATGCCAAAGGGCATGGCAAGCGCGACAGTGAAAg < 1:2783259/69‑1 (MQ=255) ttAAAGATGATCGCCATCGCGTGGCTGTGTTGGGATGCCAAAGGGCATGGCAAGCGCGACAGTGAAAg < 1:49389/68‑1 (MQ=255) tAAAGATGATCGCCATCGCGTGTCTGTGTTGGGATGCCAAAGGGCATGGCAAGCGCGACAGTGAAAGat < 1:2231598/69‑2 (MQ=255) tAAAGATGATCGCCATCGCGTGGCTGTGTTGGGATGCCAAAGGGCATGGCAAGCGCGACAGTGAAAGaa < 1:1820986/69‑1 (MQ=255) tAAAGATGATCGCCATCGCGTGGCTGTGTTGGGATGCCAAAGGGCATGGCAAGCGCGACAGTGAAAGaa < 1:2171152/69‑1 (MQ=255) tAAAGATGATCGCCATCGCGTGGCTGTGTTGGGATGCCAAAGGGCATGGCAAGCGCGACAGTGAAAGaa < 1:1044364/69‑1 (MQ=255) tAAAGATGATCGCCATCGCGTGGCTGTGTTGGGATGCCAAAGGGCATGGCAAGCGCGACAGTGAAAGaa < 1:49198/69‑1 (MQ=255) tAAAGATGATCGCCATCGCGTGGCTGTGTTGGGATGCCAAAGGGCATGGCAAGCGCGACAGTGAAAGaa < 1:1076731/69‑1 (MQ=255) tAAAGATGATCGCCATCGCGTGGCTGTGTTGGGATGCCAAAGGGCATGGCAAGCGCGACAGTGAAAGaa < 1:1545706/69‑1 (MQ=255) tAAAGATGATCGCCATCGCGTGGCTGTGTTGGGATGCCAAAGGGCATGGCAAGCGCGACAGTGAAAGaa < 1:765944/69‑1 (MQ=255) tAAAGATGATCGCCATCGCGTGGCTGTGTTGGGATGCCAAAGGGCATGGCAAGCGCGACAGTGAAAGaa < 1:129507/69‑1 (MQ=255) tAAAGATGATCGCCATCGCGTGGCTGTGTTGGGATGCCAAAGGGCATGGCAAGCGCGACAGTGAAAGaa < 1:742798/69‑1 (MQ=255) gatCGCCATCGCGTGGCTGTGTTGGGATGCCAAAGGGCATGGCAAGCGCGACAGTGAAAGAATGCAttt < 1:2089453/69‑1 (MQ=255) cATTGCGTGGCTGTGTTGGGATGCCAAAGGACACGGCAAGCGCGACAGTGAAAGAATGCATTTGATTTa < 1:159255/69‑1 (MQ=255) cATTGCGTGGCTGTGTTGGGATGCCAAAGGACACGGCAAGCGCGACAGTGAAAGAATGCATTTGATTTa < 1:2886377/69‑1 (MQ=255) cATTGCGTGGCTGTGTTGGGATGCCAAAGGACACGGCAAGCGCGACAGTGAAAGAATGCATTTGATTTa < 1:2887952/69‑1 (MQ=255) cATTGCGTGGCTGTGTTGGGATGCCAAAGGACACGGCAAGCGCGACAGTGAAAGAATGCATTTGATTTa < 1:2911093/69‑1 (MQ=255) cATTGCGTGGCTGTGTTGGGATGCCAAAGGACACGGCAAGCGCGACAGTGAAAGAATGCATTTGATTTa < 1:1278908/69‑1 (MQ=255) attgcgTGGCTGTGTTTGGATGCCAAAGGACACGGCAAGCGCGACAGTGAAAGAATGCATTTGATTTa < 1:57782/65‑1 (MQ=255) gcgTGGCTGTGTTGGGATGCCAAAGGGCATGGCAAGCGCGACAGTGAAAGAATGCATTTGa > 1:401248/1‑61 (MQ=255) gcgTGGCTGTGTTGGGATGCCAAAGGGCATGGCAAGCGCGACAGTGAAAGAATGCATTTGATTTATGaa > 1:1914710/1‑69 (MQ=255) gcgTGGCTGTGTTGGGATGCCAAAGGGCATGGCAAGCGCGACAGTGAAAGAATGCATTTGATTTATGaa > 1:1566057/1‑69 (MQ=255) gcgTGGCTGTGTTGGGATGCCAAAGGGCATGGCAAGCGCGACAGTGAAAGAATGCATTTGATTTATGaa > 1:2808333/1‑69 (MQ=255) gcgTGGCTGTGTTGGGATGCCAAAGGGCATGGCAAGCGCGACAGTGAAAGAATGCATTTGATTTATGaa > 1:456275/1‑69 (MQ=255) gcgTGGCTGTGTTGGGATGCCAAAGGGCATGGCAAGCGCGACAGTGAAAGAATGCATTTGATTTATGaa > 1:1839707/1‑69 (MQ=255) gcctGGCTGTGTTGGGATGCCAAAGGGCATGGCAAGCGCGACAGTGAAAGAATGCATTTGATTTATGaa > 1:1143362/4‑69 (MQ=255) gCTGTGTTGGGATGCCAAAGGGCATGGCAAGCGCGACAGTGAAAGAATGCATTTGATTTATGAAgttg > 1:1909977/1‑68 (MQ=255) gCTGTGTTGGGATGCCAAAGGGCATGGCAAGCGCGACAGTGAAAGAATGCATTTGATTTATGAAgttg > 1:2578675/1‑68 (MQ=255) tgtgTTGGGATGCCAAAGGGCATGGCAAGCGCGACAGTGAAAGAATGCATTTGATTTATGAAGttgttg < 1:1406689/69‑1 (MQ=255) tgtgTTGGGATGCCAAAGGGCATGGCAAGCGCGACAGTGAAAGAATGCATTTGATTTATGAAGttgttg < 1:2871893/69‑1 (MQ=255) tgtgTTGGGATGCCAAAGGGCATGGCAAGCGCGACAGTGAAAGAATGCATTTGATTTATGAAGttgttg < 1:2121707/69‑1 (MQ=255) gtgtTGGGATGCCAAAGGGCATGGCAAGCGCTACAGTGAAAGAATGCATTTGATTTATGAAGttgttg < 1:1536223/68‑1 (MQ=255) gtgtTGGGATGCCAAAGGGCATGGCAAGCGCGACAGTGAAAGAATGCATTTGATTTATGAAGttgttg < 1:2240594/68‑1 (MQ=255) gtgtTGGGATGCCAAAGGGCATGGCAAGCGCGACAGTGAAAGAATGCATTTGATTTATGAAGttgttg < 1:2415553/68‑1 (MQ=255) gtgtTGGGATGCCAAAGGGCATGGCAAGCGCGACAGTGAAAGAATGCATTTGATTTATGAAGttgttg < 1:2741853/68‑1 (MQ=255) gtgtTGGGATGCCAAAGGACACGGGAAGCGCGACAGTGAAAGAATGCATTTGATTTa < 1:3327731/57‑1 (MQ=255) gtgtCGGGATGCCAAAGGGCATGGCAAGCGCGACAGTGAAAGAATGCATTTGATTTATGAAGttgttg < 1:3254826/68‑1 (MQ=255) ttGGGATGCCAAAGGGCATGGCAAGCGCGACAGTGAAAg < 1:1685791/39‑1 (MQ=255) ttGGGATGCCAAAGGGCATGGCAAGCGCGACAGTGAAAGAATGCATTTGATTTATGAAGTTGTTGAGtt > 1:2956013/1‑69 (MQ=255) ttGGGATGCCAAAGGGCATGGCAAGCGCGACAGTGAAAGAATGCATTTGATTTATGAAGTTGTTGAGtt > 1:3127929/1‑69 (MQ=255) ttGGGATGCCAAAGGGCATGGCAAGCGCGACAGTGAAAGAATGCATTTGATTTATGAAGTTGTTGAGtt > 1:2196040/1‑69 (MQ=255) ttGGGATGCCAAAGGGCATGGCAAGCGCGACAGTGAAAGAATGCATTTGATTTATGAAGTTGTTGAGtt > 1:2659028/1‑69 (MQ=255) gggATGCCAAAGGGCATGGCAAGCGCGACAGTGAAAGAATGCa < 1:2550494/43‑1 (MQ=255) gggATGCCAAAGGGCATGGCAAGCGCGACAGTGAAAGAATGCa < 1:961843/43‑1 (MQ=255) gggATGCCAAAGGGCATGGCAAGCGCGACAGTGAAAGAATGCa < 1:2798498/43‑1 (MQ=255) gggATGCCAAAGGGCATGGCAAGCGCGACAGTGAAAGAATGCa < 1:3241600/43‑1 (MQ=255) gggATGCCAAAGGGCATGGCAAGCGCGACAGTGAAAGAATGCa < 1:3406148/43‑1 (MQ=255) gggATGCCAAAGGGCATGGCAAGCGCGACAGTGAAAGAATGCa < 1:2781074/43‑1 (MQ=255) gggATGCCAAAGGGCATGGCAAGCGCGACAGTGAAAGAATGCa < 1:1110045/43‑1 (MQ=255) gggATGCCAAAGGGCATGGCAAGCGCGACAGTGAAAGAATGCa < 1:1870055/43‑1 (MQ=255) gggATGCCAAAGGGCATGGCAAGCGCGACAGTGAAAGAATGCa < 1:1322682/43‑1 (MQ=255) gggATGCCAAAGGGCATGGCAAGCGCGACAGTGAAAGAATGCa < 1:1516604/43‑1 (MQ=255) gggATGCCAAAGGGCATGGCAAGCGCGACAGTGAAAGAATGCa < 1:2378695/43‑1 (MQ=255) gggATGCCAAAGGGCATGGCAAGCGCGACAGTGAAAGAATGCa < 1:2369080/43‑1 (MQ=255) ccAAAGGGCATGGCAAGCGCGACAGTGAAAGAATGCATTTGATTTATGAAGTTGTTGAGTTTGTAGGcc > 1:158068/1‑69 (MQ=255) ccAAAGGGCATGGCAAGCGCGACAGTGAAAGAATGCATTTGATTTATGAAGTTGTTGAGTTTGTAGGcc > 1:1845772/1‑69 (MQ=255) ccAAAGGACACGGCAAGCGCGACAGTGAAAGAATGCATTTGATTTATGa > 1:2094960/1‑49 (MQ=39) ccAAAGGACACGGCAAGCGCGACAGTGAAAGAATGCATTTGATTTATGAAGTTGTTGAGTTTGTAGGcc > 1:567063/1‑69 (MQ=255) ccAAAGGACACGGCAAGCGCGACAGTGAAAGAATGCATTTGATTTATGAAGTTGTTGAGTTTGTAGGcc > 1:3428992/1‑69 (MQ=255) ccAAAGGACACGGCAAGCGCGACAGTGAAAGAATGCATTTGATTTATGAAGTTGTTGAGTTTGTAGGcc > 1:169483/1‑69 (MQ=255) ccAAAGGACACGGCAAGCGCGACAGTGAAAGAATGCATTTGATTTATGAAGTTGTTGAGTTTGTAGGcc > 1:1663111/1‑69 (MQ=255) ccAAAGGACACGGCAAGCGCGACAGTGAAAGAATGCATTTGATTTATGAAGTTGTTGAGTTTGTAGGcc > 1:1640263/1‑69 (MQ=255) ccAAAGGACACGGCAAGCGCGACAGTGAAAGAATGCATTTGATTTATGAAGTTGTTGAGTTTGTAGGcc > 1:526993/1‑69 (MQ=255) ccAAAGGACACGGCAAGCGCGACAGTGAAAGAATGCATTTGATTTATGAAGTTGTTGAGTTTGTAGGcc > 1:1887734/1‑69 (MQ=255) ccAAAGGACACGGCAAGCGCGACAGTGAAAGAATGCATTTGATTTATGAAGTTGTTGAGTTTGTAGGcc > 1:1271362/1‑69 (MQ=255) ccAAAGGACACGGCAAGCGCGACAGTGAAAGAATGCATTTGATTTATGAAGTTGTTGAGTTTGTAGGcc > 1:1055189/1‑69 (MQ=255) ccAAAGGACACGGCAAGCGCGACAGTGAAAGAATGCATTTGATTTATGAAGTTGTTGAGTTTGTAGGcc > 1:967392/1‑69 (MQ=255) ccAAAGGACACGGCAAGCGCGACAGTGAAAGAATGCATTTGATTTATGAAGTTGTTGAGTTTGTAGGcc > 1:3327417/1‑69 (MQ=255) ccAAAGGACACGGCAAGCGCGACAGTGAAAGAATGCATTTGATTTATGAAGTTGTTGAGTTTGTAGGcc > 1:2652719/1‑69 (MQ=255) ccAAAGGACACGGCAAGCGCGACAGTGAAAGAATGCATTTGATTTATGAAGTTGTTGAGTTTGTAGGcc > 1:2599393/1‑69 (MQ=255) ccAAAGGACACGGCAAGCGCGACAGTGAAAGAATGCATTTGATTTATGAAGTTGTTGAGTTTGTAGGcc > 1:2413448/1‑69 (MQ=255) ccAAAGGACACGGCAAGCGCGACAGTGAAAGAATGCATTTGATTTATGAAGTTGTTGAGTTTGTAGGcc > 1:2913422/1‑69 (MQ=255) ccAAAGGACACGGCAAGCGCGACAGTGAAAGAATGCATTTGATTTATGAAGTTGTTGAGTTTGTAGGcc > 1:3045213/1‑69 (MQ=255) ccAAAGGACACGGCAAGCGCGACAGTGAAAGAATGCATTTGATTTATGAAGTTGTTGAGTTTGTAGGcc > 1:216100/1‑69 (MQ=255) ccAAAGGACACGGCAAGCGCGACAGTGAAAGAATGCATTTGATTTATGAAGTTGTTGAGTTTGTAGGcc > 1:3244345/1‑69 (MQ=255) ccAAAGGACACGGCAAGCGCGACAGTGAAAGAATGCATTTGATTTATGAAGTTGTTGAGTTTGTAGGcc > 1:1910939/1‑69 (MQ=255) ccAAAGGACACGGCAAGCGCGACAGTGAAAGAATGCATTTGATTTATGAAGTTGTTGAGTTTGTAGGc > 1:3173473/1‑68 (MQ=255) ccAAAGGACACGGCAAGCGCGACAGTGAAAGAATGCATTTGATTAATGAAGTTGTTGAGTTTGTAGGcc > 1:2483967/1‑69 (MQ=255) ccAAAGGACACGG‑AAGCGCGACAGTGAAAGAATGCATTTGATTTATGAAGTTGTTGAGTTTGTAGGCCg > 1:1078164/1‑69 (MQ=255) tGGCAAGCGCGACAGTGAAAGAATGCATTTGATTTATGAAGTTGTTGAGTTTGTAGGCCGCTGGTCgat < 1:2167518/69‑1 (MQ=255) tGGCAAGCGCGACAGTGAAAGAATGCATTTGATTTATGAAGTTGTTGAGTTTGTAGGCCGCTGGTCgat < 1:588887/69‑1 (MQ=255) tGGCAAGCGCGACAGTGAAAGAATGCATTTGATTTATGAAGTTGTTGAGTTTGTAGGCCGCTGGTCgat < 1:2909986/69‑1 (MQ=255) tGGCAAGCGCGACAGTGAAAGAATGCATTTGATTTATGAAGTTGTTGAGTTTGTAGGCCGCTGGTCgat < 1:135704/69‑1 (MQ=255) tGGCAAGCGCGACAGTGAAAGAATGCATTTGATTTATGAAGTTGTTGAGTTTGTAGGCCGCTGGTCgat < 1:742304/69‑1 (MQ=255) tGGCAAGCGCGACAGTGAAAGAATGCATTTGATTTATGAAGTTGTTGAGTTTGTAGGCCGCTGGTCgat < 1:1990884/69‑1 (MQ=255) tGGCAAGCGCGACAGTGAAAGAATGCATTTGATTTATGAAGTTGTTGAGTTTGTAGGCCGCTGGTCgat < 1:2615706/69‑1 (MQ=255) tGGCAAGCGCGACAGTGAAAGAATGCATTTGATTTATGAAGTTGTTGAGTTTGTACGCCGCTGGTCgat < 1:3056112/69‑1 (MQ=255) | CGTTAAAGATGATCGCCATCGCGTGGCTGTGTTGGGATGCCAAAGGGCATGGCAAGCGCGACAGTGAAAGAATGCATTTGATTTATGAAGTTGTTGAGTTTGTAGGCCGCTGGTCGAT > minE/669649‑669766 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |