BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	minE	673,313	C→T	30.5%	A562A (GCG→GCA)	ycbZ ←	predicted peptidase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	minE	673,313	0	C	T	30.5%	148.6 / 72.1	150	A562A (GCG→GCA)	ycbZ	predicted peptidase
Reads supporting (aligned to +/- strand): ref base C (65/39); new base T (28/18); total (93/57)
Fisher's exact test for biased strand distribution p-value = 8.57e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 151 total aligned reads displayed.

TAAACCAGTTCAGCCAACGTAATGGCCATGGAAAACGGTGACGTCCTTCCTGTTGCGATGCTTGCGCGATACGTTCCTGGATGGTTTGCATCAGCGTCGTTTGGCCTTCGCCATCCCAC  >  minE/673249‑673367

tAAACCAGTTCAGCCAACGTAATGGCCATGGAAAACGGTGACGTCCTTCCTGTTGCGATGCTTGCGCGa                                                    <  1:681002/69‑1 (MQ=255)

tAAACCAGTTCAGCCAACGTAATGGCCATGGAAAACGGTGACGTCCTTCCTGTTGCGATGCTTGCGCGa                                                    <  1:336057/69‑1 (MQ=255)

  aaCCAGTTCAGCCAACGTAATGGCCATGGAAAACGGTGACGTCCTTCCTGTTGCGATGCTTGCGCGATa                                                  >  1:2155644/1‑69 (MQ=255)

  aaCCAGTTCAGCCAACGTAATGGCCATGGAAAACGGTGACGTCCTTCCTGTTGCGATGCTTGCGCGATa                                                  >  1:699959/1‑69 (MQ=255)

  aaCCAGTTCAGCCAACGTAATGGCCATGGAAAACGGTGACGTCCTTCCTGTTGCGATGCTTGCGCGATa                                                  >  1:1655587/1‑69 (MQ=255)

  aaCCAGTTCAGCCAACGTAATGGCCATGGAAAACGGTGACGTCCTTCCTGTTGCGATGCTTGCGCGATa                                                  >  1:162572/1‑69 (MQ=255)

  aaCCAGTTCAGCCAACGTAATGGCCATGGAAAACGGTGACGTCCTTCCTGTTGCGATGCTTGCGCGATa                                                  >  1:1566717/1‑69 (MQ=255)

  aaCCAGTTCAGCCAACGTAATGGCCATGGAAAACGGTGACGTCCTTCCTGTTGCGATGCTTGCGCGATa                                                  >  1:1468177/1‑69 (MQ=255)

  aaCCAGTTCAGCCAACGTAATGGCCATGGAAAACGGTGACGTCCTTCCTGTTGCGATGCTTGCGCGATa                                                  >  1:2945785/1‑69 (MQ=255)

  aaCCAGTTCAGCCAACGTAATGGCCATGGAAAACGGTGACGTCCTTCCTGTTGCGATGCTTGCGCGATa                                                  >  1:1302544/1‑69 (MQ=255)

  aaCCAGTTCAGCCAACGTAATGGCCATGGAAAACGGTGACGTCCTTCCTGTTGCGATGCTTGCGCGATa                                                  >  1:932610/1‑69 (MQ=255)

  aaCCAGTTCAGCCAACGTAATGGCCATGGAAAACGGTGACGTCCTTCCTGTTGCGATGCTTGCGCGATa                                                  >  1:304640/1‑69 (MQ=255)

  aaCCAGTTCAGCCAACGTAATGGCCATGGAAAACGGTGACGTCCTTCCTGTTGCGATGCTTGCGCGATa                                                  >  1:1195771/1‑69 (MQ=255)

          cAGCCAACGTAATGGCCATGGAAAACGGTGACGTCCTTCCTGTTGCGATGCTTGCGCGATACGTTCCTg                                          >  1:2511889/1‑69 (MQ=255)

                  gTAATGGCCATGGAAAACGGTGACGTCCTTCCTGTTGCGATGCTTgcgc                                                      >  1:2205700/1‑49 (MQ=255)

                  gTAATGGCCATGGAAAACGGTGACGTCCTTCCTGTTGCGATGCTTGCGCGATACGTTCCTGGATGGtt                                   >  1:808166/1‑68 (MQ=255)

                  gTAATGGCCATGGAAAACGGTGACGTCCTTCCTGTTGCGATGCTTGCGCGATACGTTCCTGGATGGtt                                   >  1:2305209/1‑68 (MQ=255)

                  gTAATGGCCATGGAAAACGGTGACGTCCTTCCTGTTGCGATGCTTGCGCGATACGTTCCTGGATGGtt                                   >  1:2749657/1‑68 (MQ=255)

                  gTAATGGCCATGGAAAACGGTGACGTCCTTCCTGTTGCGATGCTTGCGCGATACGTTCCTGGATGGtt                                   >  1:2652304/1‑68 (MQ=255)

                     aTGGCCATGGAAAACGGTGACGTCCTTCCTGTTGCGATGCTTGCGCGATACGTTCCTGGATGGTTTGc                                >  1:3064280/1‑68 (MQ=255)

                     aTGGCCATGGAAAACGGTGACGTCCTTCCTGTTGCGATGCTTGCGCGATACGTTCCTGGATGGTTTGc                                >  1:3068209/1‑68 (MQ=255)

                     aTGGCCATGGAAAACGGTGACGTCCTTCCTGTTGCGATGCTTGCGCGATACGTTCCTGGATGGTTTGc                                >  1:3232430/1‑68 (MQ=255)

                       ggCCATGGAAAACGGTGACGTCCTTCCTGTTGCGATGCTTGCGCGATACGTTCCTGGATGGTTTGCATc                             <  1:1024568/69‑1 (MQ=255)

                        gCCATGGAAAACGGTGACGTCCTTCCTGTTGCGATGCTTGCGCGATACTTTCCTg                                          <  1:383112/55‑1 (MQ=255)

                        gCCATGGAAAACGGTGACGTCCTTCCTGTTGCGATGCTTGCGCGATACGTTCCTg                                          <  1:241140/55‑1 (MQ=255)

                        gCCATGGAAAACGGTGACGTCCTTCCTGTTGCGATGCTTGCGCGATACGTTCCTg                                          <  1:3395466/55‑1 (MQ=255)

                        gCCATGGAAAACGGTGACGTCCTTCCTGTTGCGATGCTTGCGCGATACGTTCCTg                                          <  1:536667/55‑1 (MQ=255)

                        gCCATGGAAAACGGTGACGTCCTTCCTGTTGCGATGCTTGCGCGATACGTTCCTGGATGGTTTGCATCa                            >  1:2379547/1‑69 (MQ=255)

                        gCCATGGAAAACGGTGACGTCCTTCCTGTTGCGATGCTTGCGCGATACGTTCCTGGATGGTTTGCATCa                            >  1:3290618/1‑69 (MQ=255)

                        gCCATGGAAAACGGTGACGTCCTTCCTGTTGCGATGCTTGCGCGATACGTTCCTGGATGGTTTGCATCa                            >  1:301175/1‑69 (MQ=255)

                        gCCATGGAAAACGGTGACGTCCTTCCTGTTGCGATGCTTGCGCGATACGTTCCTGGATGGTTTGCATCa                            >  1:2266038/1‑69 (MQ=255)

                        gCCATGGAAAACGGTGACGTCCTTCCTGTTGCGATGCTTGCGCGATACGTTCCTGGATGGTTTGCATCa                            >  1:1887235/1‑69 (MQ=255)

                          cATGGAAAACGGTGACGTCCTTCCTTTTGCGATGCTTGCGCGATACGTTCCTGGATGGTTTGCATCAGc                          >  1:1366810/1‑69 (MQ=255)

                          cATGGAAAACGGTGACGTCCTTCCTGTTGCGATGCTTGCGCGATACGTTCCTGGATGGTTTGCATCAGc                          >  1:2219293/1‑69 (MQ=255)

                          cATGGAAAACGGTGACGTCCTTCCTGTTGCGATGCTTGCGCGATACGTTCCTGGATGGTTTGCATCAGc                          >  1:1166610/1‑69 (MQ=255)

                          cATGGAAAACGGTGACGTCCTTCCTGTTGCGATGCTTGCGCGATACGTTCCTGGATGGTTTGCATCAGc                          >  1:957130/1‑69 (MQ=255)

                          cATGGAAAACGGTGACGTCCTTCCTGTTGCGATGCTTGCGCGATACGTTCCTGGATGGTTTGCATCAGc                          >  1:2486724/1‑69 (MQ=255)

                          cATGGAAAACGGTGACGTCCTTCCTGTTGCGATGCTTGCGCGATACGTTCCTGGATGGTTTGCATCAGc                          >  1:3011116/1‑69 (MQ=255)

                          cATGGAAAACGGTGACGTCCTTCCTGTTGCGATGCTTGCGCGATACGTTCCTGGATGGTTTGCATCAGc                          >  1:3328975/1‑69 (MQ=255)

                             ggAAAACGGTGACGTCCTTCCTGTTGCGATGCTTGCGCGATACGTTCCTg                                          <  1:642020/50‑1 (MQ=255)

                              gAAAACGGTGACGTCCTTCCTGTTGCGATGCTTGCGCGATACGTTCCTGGATGGTTTGCATCAGcgtcg                      >  1:631837/1‑69 (MQ=255)

                              gAAAACGGTGACGTCCTTCCTGTTGCGATGCTTGCGCGATACGTTCCTGGATGGTTTGCATCAGcgtcg                      >  1:3174380/1‑69 (MQ=255)

                              gAAAACGGTGACGTCCTTCCTGTTGCGATGCTTGCGCGATACGTTCCTGGATGGTTTGCATCAGcgtcg                      >  1:3201319/1‑69 (MQ=255)

                              gAAAACGGTGACGTCCTTCCTGTTGCGATGCTTGCGCGATACGTTCCTGGATGGTTTGCATCAGcgtcg                      >  1:1245714/1‑69 (MQ=255)

                              gAAAACGGTGACGTCCTTCCTGTTGCGATGCTTGCGCGATACGTTCCTGGATGGTTTGCATCAGcgtcg                      >  1:3283785/1‑69 (MQ=255)

                              gAAAACGGTGACGTCCTTCCTGTTGCGATGCTTGCGCGATACGTTCCTGGATGGTTTGCATCAGcgtcg                      >  1:281213/1‑69 (MQ=255)

                              gAAAACGGTGACGTCCTTCCTGTTGCGATGCTTGCGCGATACGTTCCTGGATGGTTTGCATCAGcgtcg                      >  1:2719519/1‑69 (MQ=255)

                              gAAAACGGTGACGTCCTTCCTGTTGCGATGCTTGCGCGATACGTTCCTGGATGGTTTGCATCAGcgtcg                      >  1:1630018/1‑69 (MQ=255)

                              gAAAACGGTGACGTCCTTCCTGTTGCGATGCTTGCGCGATACGTTCCTGGATGGTTTGCATCAGcgtcg                      >  1:1851744/1‑69 (MQ=255)

                              gAAAACGGTGACGTCCTTCCTGTTGCGATGCTTGCGCGATACGTTCCTGGATGGTTTGCATCAGcgtcg                      >  1:2491967/1‑69 (MQ=255)

                              gAAAACGGTGACGTCCTTCCTGTTGCGATGCTTGCGCGATACGTTCCTGGATGGTTTGCATCAGcgtcg                      >  1:2675894/1‑69 (MQ=255)

                              gAAAACGGTGACGTCCTTCCTGTTGCGATGCTTGCGCGATACGTTCCTGGATGGTTTGCATCAGcgtc                       >  1:782132/1‑68 (MQ=255)

                              gAAAACGGTGACGTCCTTCCTGTTGCGATGCTTGCGCGATACGTTCCTGGATGATTTGCATCAGcgtcg                      >  1:832857/1‑69 (MQ=255)

                              gAAAACGGTGACGTCCTT‑CTGTTGCGATGCTTGCGCGATACGTTCCTGGATGGTTTGCATCAGcgtcgt                     >  1:3404740/1‑69 (MQ=255)

                                aaaCGGTGACGTCCTTCCTGTTGCGATGCTTGCGCGATACGTTCCTGGATGGTTTGCATCAGcgtcgt                     <  1:2903625/68‑1 (MQ=255)

                                aaaCGGTGACGTCCTTCCTGTTGCGATGCTTGCGCGATACGTTCCTGGATGGTTTGCATCAGcgtcgt                     <  1:3429949/68‑1 (MQ=255)

                                aaaCGGTGACGTCCTTCCTGTTGCGATGCTTGCGCGATACGTTCCTGGATGGTTTGCATCAGcgtcgt                     <  1:3433666/68‑1 (MQ=255)

                                aaaCGGTGACGTCCTTCCTGTTGCGATGCTTGCGCGATACGTTCCTGGATGGTTTGCATCAGcgtcgt                     <  1:1662711/68‑1 (MQ=255)

                                aaaCGGTGACGTCCTTCCTGTTGCGATGCTTGCGCGATACGTTCCTGGATGGTTTGCATCAGcgtcgt                     <  1:714901/68‑1 (MQ=255)

                                aaaCGGTGACGTCCTTCCTGTTGCGATGCTTGCGCGATACGTTCCTGGATGGTTTGCATCAGcgtcgt                     <  1:1524948/68‑1 (MQ=255)

                                aaaCGGTGACGTCCTTCCTGTTGCGATGCTTGCGCGATACGTTCCTGGATGGTTTGCATCAGcgtcgt                     <  1:219194/68‑1 (MQ=255)

                                  aCGGTGACGTCCTTCCTGTTGCGATGCTTGCGCGATACGTTCCTGGATGGTTTGCATCAGCGTCGTTTg                  <  1:2935415/69‑1 (MQ=255)

                                   cGGTGACGTCCTTCCTGTTGCGATGCTTGTGCGATACGTTCCTGGATTGttt                                  >  1:2619319/1‑52 (MQ=255)

                                   cGGTGACGTCCTTCCTGTTGCGATGCTTGTGCGATACGTTCCTGGATGGTTTg                                 >  1:733893/1‑53 (MQ=255)

                                   cGGTGACGTCCTTCCTGTTGCGATGCTTGTGCGATACGTTCCTGGATGGTTTg                                 >  1:772175/1‑53 (MQ=255)

                                   cGGTGACGTCCTTCCTGTTGCGATGCTTGTGCGATACGTTCCTGGATGGTTTg                                 >  1:433603/1‑53 (MQ=255)

                                   cGGTGACGTCCTTCCTGTTGCGATGCTTGTGCGATACGTTCCTGGATGGTTTg                                 >  1:961759/1‑53 (MQ=255)

                                   cGGTGACGTCCTTCCTGTTGCGATGCTTGTGCGATACGTTCCTGGATGGTTTg                                 >  1:978387/1‑53 (MQ=255)

                                   cGGTGACGTCCTTCCTGTTGCGATGCTTGTGCGATACGTTCCTGGATGGTTTg                                 >  1:1423708/1‑53 (MQ=255)

                                   cGGTGACGTCCTTCCTGTTGCGATGCTTGTGCGATACGTTCCTGGATGGTTTg                                 >  1:2948601/1‑53 (MQ=255)

                                   cGGTGACGTCCTTCCTGTTGCGATGCTTGTGCGATACGTTCCTGGATGGTTTg                                 >  1:2926465/1‑53 (MQ=255)

                                   cGGTGACGTCCTTCCTGTTGCGATGCTTGTGCGATACGTTCCTGGATGGTTTg                                 >  1:2771933/1‑53 (MQ=255)

                                   cGGTGACGTCCTTCCTGTTGCGATGCTTGTGCGATACGTTCCTGGATGGTTTg                                 >  1:260799/1‑53 (MQ=255)

                                   cGGTGACGTCCTTCCTGTTGCGATGCTTGTGCGATACGTTCCTGGATGGTTTg                                 >  1:186356/1‑53 (MQ=255)

                                   cGGTGACGTCCTTCCTGTTGCGATGCTTGTGCGATACGTTCCTGGATGGTTTg                                 >  1:2025383/1‑53 (MQ=255)

                                   cGGTGACGTCCTTCCTGTTGCGATGCTTGTGCGATACGTTCCTGGATGGTTTg                                 >  1:2043159/1‑53 (MQ=255)

                                     gTGACGTCCTTCCTGTTGCGATGCTTGTGCGATACGTTCCTGGATGGTTTGCATCAGCGTCGTTTGGcc               <  1:3127411/69‑1 (MQ=255)

                                     gTGACGTCCTTCCTGTTGCGATGCTTGTGCGATACGTTCCTGGATGGTTTGCATCAGCGTCGTTTGGcc               <  1:1493030/69‑1 (MQ=255)

                                     gTGACGTCCTTCCTGTTGCGATGCTTGTGCGATACGTTCCTGGATGGTTTGCATCAGCGTCGTTTGGcc               <  1:17033/69‑1 (MQ=255)

                                     gTGACGTCCTTCCTGTTGCGATGCTTGTGCGATACGTTCCTGGATGGTTTGCATCAGCGTCGTTTGGcc               <  1:1863095/69‑1 (MQ=255)

                                     gTGACGTCCTTCCTGTTGCGATGCTTGTGCGATACGTTCCTGGATGGTTTGCATCAGCGTCGTTTGGcc               <  1:1315432/69‑1 (MQ=255)

                                     gTGACGTCCTTCCTGTTGCGATGCTTGTGCGATACGTTCCTGGATGGTTTGCATCAGCGTCGTTTGGcc               <  1:1979762/69‑1 (MQ=255)

                                     gTGACGTCCTTCCTGTTGCGATGCTTGTGCGATACGTTCCTGGATGGTTTGCATCAGCGTCGTTTGGcc               <  1:2328469/69‑1 (MQ=255)

                                     gTGACGTCCTTCCTGTTGCGATGCTTGTGCGATACGTTCCTGGATGGTTTGCATCAGCGTCGTTTGGcc               <  1:2558921/69‑1 (MQ=255)

                                     gTGACGTCCTTCCTGTTGCGATGCTTGTGCGATACGTTCCTGGATGGTTTGCATCAGCGTCGTTTGGcc               <  1:2884557/69‑1 (MQ=255)

                                     gTGACGTCCTTCCTGTTGCGATGCTTGTGCGATACGTTCCTGGATGGTTTGCATCAGCGTCGTTTGGcc               <  1:3043131/69‑1 (MQ=255)

                                     gTGACGTCCTTCCTGTTGCGATGCTTGTGCGATACGTTCCTGGATGGTTTGCATCAGCGTCGTTTGGcc               <  1:3101640/69‑1 (MQ=255)

                                     gTGACGTCCTTCCTGTTGCGATGCTTGCGCGATACGTTCCTg                                          <  1:2281054/42‑1 (MQ=255)

                                     gTGACGTCCTTCCTGTTGCGATGCTTGCGCGATACGTTCCTGGATGGTTTGCATCAGCGTCGTTTGGcc               <  1:558213/69‑1 (MQ=255)

                                     gTGACGTCCTTCCTGTTGCGATGCTTGCGCGATACGTTCCTGGATGGTTTGCATCAGCGTCGTTTGGcc               <  1:1160885/69‑1 (MQ=255)

                                     gTGACGTCCTTCCTGTTGCGATGCTTGCGCGATACGTTCCTGGATGGTTTGCATCAGCGTCGTTTGGcc               <  1:1379006/69‑1 (MQ=255)

                                      tGACGTCCTTCCTGTTGCGATGCTTGCGCGATACGTTCCTGGATGGTTTGCATCAGCGTCGTTTGGcc               <  1:3219752/68‑1 (MQ=255)

                                       gACGTCCTTCCTGTTGCGATGCTTGCGCGATACGTTCCTGGATGGTTTGCATCAGCGTCGTTTg                  <  1:1356469/64‑1 (MQ=255)

                                       gACGTCCTTCCTGTTGCGATGCTTGCGCGATACGTTCCTGGATGGTTTGCATCAGCGTCGTTTGGCCtt             >  1:28283/1‑69 (MQ=255)

                                          gTCCTTCCTGTTGCGATGCTTGCGCGATACGTTCCTGGATGGTTTGCATCAGCGTCGTTTGGCCTTCGc          <  1:1216401/69‑1 (MQ=255)

                                                cctGTTGCGATGCTTGCGCGATACGTTCCTGGATGGTTTGCATCAGcgtcgt                     <  1:1246449/52‑1 (MQ=255)

                                                      gCGATGCTTGCGCGATACGTTCCTGGATGGTTTGCATCAGCGTCGtt                    >  1:683451/1‑47 (MQ=255)

                                                      gCGATGCTTGCGCGATACGTTCCTGGATGGTTTGCATCAGCGTCGTTTGGCCTTCGCCATCCcac  >  1:732816/1‑65 (MQ=255)

                                                      gCGATGCTTGCGCGATACGTTCCTGGATGGTTTGCATCAGCGTCGTTTGGCCTTCGCCATCCcac  >  1:872168/1‑65 (MQ=255)

                                                      gCGATGCTTGCGCGATACGTTCCTGGATGGTTTGCATCAGCGTCGTTTGGCCTTCGCCATCCcac  >  1:1005359/1‑65 (MQ=255)

TAAACCAGTTCAGCCAACGTAATGGCCATGGAAAACGGTGACGTCCTTCCTGTTGCGATGCTTGCGCGATACGTTCCTGGATGGTTTGCATCAGCGTCGTTTGGCCTTCGCCATCCCAC  >  minE/673249‑673367

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 21 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`