| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 675,925 | G→A | 32.4% | R276R (CGC→CGT) | ompA ← | outer membrane protein A (3a;II*;G;d) |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 675,925 | 0 | G | A | 32.4% | 71.8 / 40.8 | 83 | R276R (CGC→CGT) | ompA | outer membrane protein A (3a;II*;G;d) |
| Reads supporting (aligned to +/- strand): ref base G (22/34); new base A (15/12); total (37/46) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.38e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
CCACGTGCGGAGATCTTGTCTGCCGGGATACCTTTGGAGATCAGGTAATCAACAACAGACTGAGCACGGCGCTCGGACAGACCCTGGTTGTAAGCGTCAGAACCGATGCGGTCGGTGTAACCCAGAACAACTACGGA > minE/675857‑675993 | ccACGTGCGGAGATCTTGTCTGCCGGGATACCTTTGGAGATCAGGTAATCAACAACAGACTTAGCACgg > 1:2568979/1‑69 (MQ=255)ccACGTGCGGAGATCTTGTCTGCCGGGATACCTTTGGAGATCAGGTAATCAACAACAGACTGAGCACgg > 1:904126/1‑69 (MQ=255)ccACGTGCGGAGATCTTGTCTGCCGGGATACCTTTGGAGATCAGGTAATCAACAACAGACTGAGCACgg > 1:2736552/1‑69 (MQ=255)ccACGTGCGGAGATCTTGTCTGCCGGGATACCTTTGGAGATCAGGTAATCAACAACAGACTGAGCACgg > 1:1228900/1‑69 (MQ=255)ccACGTGCGGAGATCTTGTCTGCCGGGATACCTTTGGAGATCAGGTAATCAACAACAGACTGAGCACgg > 1:1898845/1‑69 (MQ=255)ccACGTGCGGAGATCTTGTCTGCCGGGATACCTTTGGAGATCAGGTAATCAACAACAGACTGAGCACgg > 1:2430053/1‑69 (MQ=255)ccACGTGCGGAGATCTTGTCTGCCGGGATACCTTTGGAGATCAGGTAATCAACAACAGACTGAGCACgg > 1:1997802/1‑69 (MQ=255)ccACGTGCGGAGATCTTGTCTGCCGGGATACCTTTGGAGATCAGGTAATCAACAACAGACTGAGCACgg > 1:3145640/1‑69 (MQ=255) gagaTCTTGTCTGCCGGGATACCTTTGGAGATCAGGTAATCACCAACAGACTGAGCACGGCGCTCg > 1:3159652/1‑66 (MQ=255) aTCTTGTCTGCCGGGATACCTTTGGAGATCAGGTAATCAACAACAGACTGAGCACGGCGCTCGGACAGa < 1:1154544/69‑1 (MQ=255) aTCTTGTCTGCCGGGATACCTTTGGAGATCAGGTAATCAACAACAGACTGAGCACGGCGCTCGGACAGa < 1:728629/69‑1 (MQ=255) tCTTGTCTGCCGGGATACCTTTGGAGATCAGGTAATCAACAACAGACTGAGCACGGCGCTCGGACAGAc < 1:543566/69‑1 (MQ=255) cTTGTCTGCCGGGATACCTTTGGAGATCAGGTAATCAACAACAGACTGAGCACGGCGCTCGGACAGAc < 1:505182/68‑1 (MQ=255) gggATACCTTTAGAGATCAGGTAATCAACAACGGACTGAGCACGACGCTCGGACAGAGCCTGGtta < 1:2262365/66‑2 (MQ=255) gggATACCTTTAGAGATCAGGTAATCAACAACGGACTGAGCACGACGCTCGGACAGAGCCTGGtta < 1:2909050/66‑2 (MQ=255) gggATACCTTTAGAGATCAGGTAATCAACAACGGACTGAGCACGACGCTCGGACAGAGCCTGGtta < 1:1986338/66‑2 (MQ=255) gggATACCTTTAGAGATCAGGTAATCAACAACGGACTGAGCACGACGCTCGGACAGAGCCTGGtta < 1:2821477/66‑2 (MQ=255) gggATACCTTTAGAGATCAGGTAATCAACAACGGACTGAGCACGACGCTCGGACAGAGCCTGGtta < 1:1929386/66‑2 (MQ=255) gggATACCTTTAGAGATCAGGTAATCAACAACGGACTGAGCACGACGCTCGGACAGAGCCTGGtta < 1:1672858/66‑2 (MQ=255) gggATACCTTTAGAGATCAGGTAATCAACAACGGACTGAGCACGACGCTCGGACAGAGCCTGGtta < 1:570502/66‑2 (MQ=255) gggATACCTTTAGAGATCAGGTAATCAACAACGGACTGAGCACGACGCTCGGACAGAGCCTGGtta < 1:1261276/66‑2 (MQ=255) gggATACCTTTAGAGATCAGGTAATCAACAACGGACTGAGCACGACGCTCGGACAGAGCCTGGtta < 1:1551535/66‑2 (MQ=255) gggATACCTTTAGAGATAAGGTAATCAACAACGGACTGAGCACGACGCTCAGACAGAGCCTGGtta < 1:541442/66‑2 (MQ=255) ccTTTGGAGATCAGGTAATCAACAACAGACTGAGCACGGCGCTCGGACAGACCCTGGTTGTAAGCGTc > 1:2704605/1‑68 (MQ=255) ccTTTGGAGATCAGGTAATCAACAACAGACTGAGCACGGCGCTCGGACAGACCCTGGTTGTAAGCGTc > 1:194314/1‑68 (MQ=255) ccTTTGGAGATCAGGTAATCAACAACAGACTGAGCACGGCGCTCGGACAGACCCTGGTTGTAAGCGTc > 1:1288734/1‑68 (MQ=255) ccTTTGGAGATCAGGTAATCAACAACAGACTGAGCACGGCGCTCGGACAGACCCTGGTTGTAAGCGTc > 1:2957978/1‑68 (MQ=255) ccTTTGGAGATCAGGTAATCAACAACAGACTGAGCACGGCGCTCGGACAGACCCTGGTTGTAAGCGTc > 1:2048330/1‑68 (MQ=255) tttGGAGATCAGGTAATCAACAACAGACTGAGCACGGCGCTCGGACAGACCCTGGTTGTAAGCGTCAg < 1:983244/68‑1 (MQ=255) tttGGAGATCAGGTAATCAACAACAGACTGAGCACGGCGCTCGGACAGACCCTGGTTGTAAGCGTCAg < 1:900529/68‑1 (MQ=255) tttGGAGATCAGGTAATCAACAACAGACTGAGCACGGCGCTCGGACAGACCCTGGTTGTAAGCGTCAg < 1:1321393/68‑1 (MQ=255) tttGGAGATCAGGTAATCAACAACAGACTGAGCACGGCGCTCGGACAGACCCTGGTTGTAAGCGTCAg < 1:3127624/68‑1 (MQ=255) tttGGAGATCAGGTAATCAACAACAGACTGAGCACGGCGCTCGGACAGACCCTGGTTGTAAGCGTCAg < 1:524226/68‑1 (MQ=255) tttGGAGATCAGGTAATCAACAACAGACTGAGCACGGCGCTCGGACAGACCCTGGTTGTAAGCGTCAg < 1:3209340/68‑1 (MQ=255) tttGGAGATCAGGTAATCAACAACAGACTGAGCACGGCGCTCGGACAGACCCTGGTTGTAAGCGTCAg < 1:395887/68‑1 (MQ=255) tttGGAGATCAGGTAATCAACAACAGACTGAGCACGGCGCTCGGACAGACCCTGGTTGTAAGCGTCAg < 1:50809/68‑1 (MQ=255) tttGGAGATCAGGTAATCAACAACAGACTGAGCACGGCGCTCGGACAGACCCTGGTTGTAAGCGTCAg < 1:1655509/68‑1 (MQ=255) ttGGAGATCAGGTAATCAACAACAGACTGAGCACGGCGCTCGGACAGACCCTGGTTGTAAGCGTCAg < 1:2898122/67‑1 (MQ=255) ggAGATCAGGTAATCAACAACAGACTGAGCACGGCGCTCGGACAGACCCTGGTTGTAAGCGTCAg < 1:1910114/65‑1 (MQ=255) gaTCAGGTAATCAACAACAGACTGAGCACGGCGCTCGGACAGACCCTGGTTGTAAGCGTCAGAACCGAt < 1:1929403/69‑1 (MQ=255) cAGGTAATCAACAACAGACTGAGCACGGCGCTCGGACAGACCCTGGTTGTa < 1:3123330/51‑1 (MQ=255) cAGGTAATCAACAACAGACTGAGCACGGCGCTCGGACAGACCCTGGTTGTa < 1:2962861/51‑1 (MQ=255) cAGGTAATCAACAACAGACTGAGCACGGCGCTCGGACAGACCCTGGTTGTa < 1:2900348/51‑1 (MQ=255) cAGGTAATCAACAACAGACTGAGCACGGCGCTCGGACAGACCCTGGTTGTa < 1:263568/51‑1 (MQ=255) cAGGTAATCAACAACAGACTGAGCACGGCGCTCGGACAGACCCTGGTTGTa < 1:501626/51‑1 (MQ=255) cAGGTAATCAACAACAGACTGAGCACGGCGCTCGGACAGACCCTGGTTGTa < 1:1060715/51‑1 (MQ=255) cAGGTAATCAACAACAGACTGAGCACGGCGCTCGGACAGACCCTGGTTGTa < 1:1321615/51‑1 (MQ=255) cAGGTAATCAACAACAGACTGAGCACGGCGCTCGGACAGACCCTGGTTGTa < 1:1340432/51‑1 (MQ=255) cAGGTAATCAACAACAGACTGAGCACGGCGCTCGGACAGACCCTGGTTGTa < 1:2062254/51‑1 (MQ=255) cAGGTAATCAACAACAGACTGAGCACGGCGCTCGGACAGACCCTGGTTGTa < 1:2502578/51‑1 (MQ=255) cAGGTAATCAACAACAGACTGAGCACGGCGCTCGGACAGACCCTGGTTGTa < 1:2158529/51‑1 (MQ=255) cAGGTAATCAACAACAGACTGAGCACGGCGCTCGGACAGACCCT‑GTTGTa < 1:2442357/50‑1 (MQ=255) gTAATCAACAACGGACTGAGCACGACGCTCGGACAGAGCCTGGtta < 1:3178384/46‑2 (MQ=255) tAATCAACAACGGACTGAGCACGACGCTCGGACAGAGCCTGGtta < 1:2754855/45‑2 (MQ=255) aTCAACAACGGACTGAGCACGACGCTCGGACAGAGCCTg > 1:354932/1‑39 (MQ=37) aTCAACAACGGACTGAGCACGACGCTCGGACAGAGCCTGGTTATAAGCGTCAGAACCGATGcggtcag > 1:1023313/1‑66 (MQ=255) aTCAACAACGGACTGAGCACGACGCTCGGACAGAGCCTGGTTATAAGCGTCAGAACCGATGcggtcag > 1:3249370/1‑66 (MQ=255) aTCAACAACGGACTGAGCACGACGCTCGGACAGAGCCTGGTTATAAGCGTCAGAACCGATGcggtcag > 1:1433699/1‑66 (MQ=255) aTCAACAACGGACTGAGCACGACGCTCGGACAGAGCCTGGTTATAAGCGTCAGAACCGATGcggtcag > 1:2692606/1‑66 (MQ=255) aTCAACAACGGACTGAGCACGACGCTCGGACAGAGCCTGGTTATAAGCGTCAGAACCGATGcggtcag > 1:1310775/1‑66 (MQ=255) aTCAACAACGGACTGAGCACGACGCTCGGACAGAGCCTGGTTATAAGCGTCAGAACCGATGcggtcag > 1:221610/1‑66 (MQ=255) aTCAACAACGGACTGAGCACGACGCTCGGACAGAGCCTGGTTATAAGCGTCAGAACCGATGcggtcag > 1:2234867/1‑66 (MQ=255) aTCAACAACGGACTGAGCACGACGCTCGGACAGAGCCTGGTTATAAGCGTCAGAACCGATGcggtcag > 1:3027224/1‑66 (MQ=255) aTCAACAACGGACTGAGCACGACGCTCGGACAGAGCCTGGTTATAAGCGTCAGAACCGATGcggtcag > 1:3009181/1‑66 (MQ=255) aTCAACAACGGACTGAGCACGACGCTCGGACAGAGCCTGGTTATAAGCGTCAGAACCGATGcggtcag > 1:2981108/1‑66 (MQ=255) aTCAACAACGGACTGAGCACGACGCTCGGACAGAGCCTGGTTATAAGCGTCAGAACCGATGcggtcag > 1:1197877/1‑66 (MQ=255) aTCAACAACGGACTGAGCACGACGCTCGGACAGAGCCTGGTTATAAGCGTCAGAACCGATGcggtcag > 1:228046/1‑66 (MQ=255) aTCAACAACGGACTGAGCACGACGCTCGGACAGAGCCTGGTTATAAGCGTCAGAACCGATGcggtcag > 1:2289707/1‑66 (MQ=255) aTCAACAACGGACTGAGCACGACGCTCGGACAGAGCCTGGTTATAAGCGTCAGAACCGATGcggtcag > 1:1130608/1‑66 (MQ=255) aacaacAGACTGAGCACGGCGCTCGGACAGACCCTGGTTGTAAGCGTCAGAACCGATGCGGTCGGTGTa > 1:736396/1‑69 (MQ=255) aacaacAGACTGAGCACGGCGCTCGGACAGACCCTGGTTGTAAGCGTCAGAACCGATGCGGTCGGTGTa > 1:849164/1‑69 (MQ=255) aacaacAGACTGAGCACGGCGCTCGGACAGACCCTGGTTGTAAGCGTCAGAACCGATGCGGTCGGTGTa > 1:946280/1‑69 (MQ=255) aacaacAGACTGAGCACGGCGCTCGGACAGACCCTGGTTGTAAGCGTCAGAACCGATGCGGTCGGTGTa > 1:1666385/1‑69 (MQ=255) aacaacAGACTGAGCACGGCGCTCGGACAGACCCTGGTTGTAAGCGTCAGAACCGATGCGGTCGGTGTa > 1:1822060/1‑69 (MQ=255) aacaacAGACTGAGCACGGCGCTCGGACAGACCCTGGTTGTAAGCGTCAGAACCGATGCGGTCGGTGTa > 1:462452/1‑69 (MQ=255) aacaacAGACTGAGCACGGCGCTCGGACAGACCCTGGTTGTAAGCGTCAGAACCGATGCGGTCGGTGTa > 1:2421040/1‑69 (MQ=255) aacaacAGACTGAGCACGGCGCTCGGACAGACCCTGGTTGTAAGCGTCAGAACCGATGCGGTCGGTGTa > 1:2481059/1‑69 (MQ=255) aacaacAGACTGAGCACGGCGCTCGGACAGACCCTGGTTGTAAGCGTCAGAACCGATGCGGTCGGTGTa > 1:2466465/1‑69 (MQ=255) aacaacAGACTGAGCACGGCGCTCGGACAGACCCTGGTTGTAAGCGTCAGAACCGATGCGGTCGGTGTa > 1:2295642/1‑69 (MQ=255) caacaGACTGAGCACGGCGCTCGGACAGACCCTGGTTGTAAGCGTCAGAACCGATGCGGTCGGTGTAAc < 1:1253775/69‑1 (MQ=255) caacaGACTGAGCACGGCGCTCGGACAGACCCTGGTTGTAAGCGTCAGAACCGATGCGGTCGGTGTAAc < 1:2278233/69‑1 (MQ=255) caacaGACTGAGCACGGCGCTCGGACAGACCCTGGTTGTAAGCGTCAGAACCGATGCGGTCGGTGTAAc < 1:2981404/69‑1 (MQ=255) cTGAGCACGGCGCTCGGACAGACCCTGGTTGTAAGCGTCAGAACCg > 1:2968604/1‑46 (MQ=255) cTGAGCACGGCGCTCGGACAGACCCTGGTTGTAAGCGTCAGAACCGATGCGGTCGGTGTAAcc > 1:3310487/1‑63 (MQ=255) cTGAGCACGGCGCTCGGACAGACCCTGGTTGTAAGCGTCAGAACCGATGCGGTCGGTGTAACCCAGaac > 1:3353048/1‑69 (MQ=255) cTGAGCACGGCGCTCGGACAGACCCTGGTTGTAAGCGTCAGAACCGATGCGGTCGGTGTAACCCAGaac > 1:2350235/1‑69 (MQ=255) cTGAGCACGGCGCTCGGACAGACCCTGGTTGTAAGCGTCAGAACCGATGCGGTCGGTGTAACCCAGaac > 1:2643324/1‑69 (MQ=255) cTGAGCACGGCGCTCGGACAGACCCTGGTTGTAAGCGTCAGAACCGATGCGGTCGGTGTAACCCAGaac > 1:2036517/1‑69 (MQ=255) cACGGCGCTCGGACAGACCCTGGTTGTAAGCGTCAGAACCGATGCGGTCGGTGTAACCCAGaacaac < 1:1256011/67‑1 (MQ=255) cACGGCGCTCGGACAGACCCTGGTTGTAAGCGTCAGAACCGATGCGGTCGGTGTAACCCAGaacaac < 1:917664/67‑1 (MQ=255) aCGGCGCTCGGACAGACCCTGGTTGTAAGCGTCAGAACCGATGCGGTCGGTGTAACCCAGaacaac < 1:2888098/66‑1 (MQ=255) gcgcTCGGACAGACCCTGGTTGTAAGCGTCAGAACCGATGCGGTCGGTGTAACCCAGAACAACTACGGa > 1:3163070/1‑69 (MQ=255) gcgcTCGGACAGACCCTGGTTGTAAGCGTCAGAACCGATGCGGTCGGTGTAACCCAGAACAACTACGGa > 1:3304523/1‑69 (MQ=255) | CCACGTGCGGAGATCTTGTCTGCCGGGATACCTTTGGAGATCAGGTAATCAACAACAGACTGAGCACGGCGCTCGGACAGACCCTGGTTGTAAGCGTCAGAACCGATGCGGTCGGTGTAACCCAGAACAACTACGGA > minE/675857‑675993 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |