Predicted mutation
evidence seq id position mutation freq annotation gene description
RA minE 69,870 T→C 23.1% L255L (TTG→CTG)  leuO → DNA‑binding transcriptional activator

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*minE69,8700TC23.1% 162.0 / 41.8 125L255L (TTG→CTG) leuODNA‑binding transcriptional activator
Reads supporting (aligned to +/- strand):  ref base T (73/23);  new base C (7/22);  total (80/45)
Fisher's exact test for biased strand distribution p-value = 9.14e-07
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.95e-01

Only 100 of 126 total aligned reads displayed.

TCGCGTATCAGGGCATGGCAATGATGAGCGTACTTAGCGTGGTGTCGCAAACGCATTTGGTCGCTATTGCGCCGCGTTGGCTGGCTGAAGAGTTCGCTGAATCCTTAGAATTACAGGT  >  minE/69814‑69931
                                                        |                                                             
tCGCGTATCAGGGCATGGCAATGATGAGCGTACTTAGCGTGGTGTCGCAAACGCATTTGGTCGc                                                        >  1:1024123/1‑64 (MQ=255)
tCGCGTATCAGGGCATGGCAATGATGAGCGTACTTAGCGTGGTGTCGCAAACGCATTTGGTCGc                                                        >  1:1877614/1‑64 (MQ=255)
tCGCGTATCAGGGCATGGCAATGATGAGCGTACTTAGCGTGGTGTCGCAAACGCATTTGGTCGc                                                        >  1:780606/1‑64 (MQ=255)
tCGCGTATCAGGGCATGGCAATGATGAGCGTACTTAGCGTGGTGTCGCAAACGCATTTGGTCGc                                                        >  1:1154170/1‑64 (MQ=255)
tCGCGTATCAGGGCATGGCAATGATGAGCGTACTTAGCGTGGTGTCGCAAACGCATTTGGTCGc                                                        >  1:1593524/1‑64 (MQ=255)
tCGCGTATCAGGGCATGGCAATGATGAGCGTACTTAGCGTGGTGTCGCAAACGCATTTGGTCGc                                                        >  1:1281472/1‑64 (MQ=255)
tCGCGTATCAGGGCATGGCAATGATGAGCGTACTTAGCGTGGTGTCGCAAACGCATTTGGTCGc                                                        >  1:1318662/1‑64 (MQ=255)
tCGCGTATCAGGGCATGGCAATGATGAGCGTACTTAGCGTGGTGTCGCAAACGCATTTGGTCGc                                                        >  1:2719879/1‑64 (MQ=255)
tCGCGTATCAGGGCATGGCAATGATGAGCGTACTTAGCGTGGTGTCGCAAACGCATTTGGTCGCTATTg                                                   >  1:2079077/1‑69 (MQ=255)
tCGCGTATCAGGGCATGGCAATGATGAGCGTACTTAGCGTGGTGTCGCAAACGCATTTGGTCGCTATTg                                                   >  1:1549319/1‑69 (MQ=255)
tCGCGTATCAGGGCATGGCAATGATGAGCGTACTTAGCGTGGTGTCGCAAACGCATTTGGTCGCTATTg                                                   >  1:667484/1‑69 (MQ=255)
tCGCGTATCAGGGCATGGCAATGATGAGCGTACTTAGCGTGGTGTCGCAAACGCATTTGGTCGCTATTg                                                   >  1:3070644/1‑69 (MQ=255)
         aGGGCATGGCAATGATGAGCGTACTTAGCGTGGTGTCGCAAACGCATTTGGTCGCTATTGCGCCGCGtt                                          >  1:3156868/1‑69 (MQ=255)
         aGGGCATGGCAATGATGAGCGTACTTAGCGTGGTGTCGCAAACGCATTTGGTCGCTATTGCGCCGCGtt                                          >  1:3296556/1‑69 (MQ=255)
         aGGGCATGGCAATGATGAGCGTACTTAGCGTGGTGTCGCAAACGCATTTGGTCGCTATTGCGCCGCGtt                                          >  1:41006/1‑69 (MQ=255)
         aGGGCATGGCAATGATGAGCGTACTTAGCGTGGTGTCGCAAACGCATTTGGTCGCTATTGCGCCGCGtt                                          >  1:151630/1‑69 (MQ=255)
         aGGGCATGGCAATGATGAGCGTACTTAGCGTGGTGTCGCAAACGCATTTGGTCGCTATTGCGCCGCGtt                                          >  1:505441/1‑69 (MQ=255)
         aGGGCATGGCAATGATGAGCGTACTTAGCGTGGTGTCGCAAACGCATTTGGTCGCTATTGCGCCGCGtt                                          >  1:1359492/1‑69 (MQ=255)
         aGGGCATGGCAATGATGAGCGTACTTAGCGTGGTGTCGCAAACGCATTTGGTCGCTATTGCGCCGCGtt                                          >  1:1259924/1‑69 (MQ=255)
         aGGGCATGGCAATGATGAGCGTACTTAGCGTGGTGTCGCAAACGCATTTGGTCGCTATTGCGCCGCGtt                                          >  1:2955176/1‑69 (MQ=255)
         aGGGCATGGCAATGATGAGCGTACTTAGCGTGGTGTCGCAAACGCATTTGGTCGCTATTGCGCCGCGtt                                          >  1:737161/1‑69 (MQ=255)
         aGGGCATGGCAATGATGAGCGTACTTAGCGTGGTGTCGCAAACGCATTTGGTCGCTATTGCGCCGCGtt                                          >  1:912223/1‑69 (MQ=255)
         aGGGCATGGCAATGATGAGCGTACTTAGCGTGGTGTCGCAAACGCATTTGGTCGCTATTGCGCCGCGtt                                          >  1:2800399/1‑69 (MQ=255)
         aGGGCATGGCAATGATGAGCGTACTTAGCGTGGTGTCGCAAACGCATTTGGTCGCTATTGCGCCGCGt                                           >  1:3383019/1‑68 (MQ=255)
            gCATGGCAATGATGAGCGTACTTAGCGTGGTGTCGCAAACGCATCTGGTCGCTATTGCGCCGCGTtggt                                       >  1:1488496/1‑68 (MQ=255)
            gCATGGCAATGATGAGCGTACTTAGCGTGGTGTCGCAAACGCATCTGGTCGCTATTGCGCCGCGTtggt                                       >  1:3110800/1‑68 (MQ=255)
            gCATGGCAATGATGAGCGTACTTAGCGTGGTGTCGCAAACGCATCTGGTCGCTATTGCGCCGCGTtggt                                       >  1:1670075/1‑68 (MQ=255)
            gCATGGCAATGATGAGCGTACTTAGCGTGGTGTCGCAAACGCATCTGGTCGCTATTGCGCCGCGTtggt                                       >  1:1073970/1‑68 (MQ=255)
            gCATGGCAATGATGAGCGTACTTAGCGTGGTGTCGCAAACGCATCTGGTCGCTATAGCGCCGCGTtggt                                       >  1:1504742/1‑68 (MQ=255)
                 gCAATGATGAGCGTACTTAGCGTGGTGTCGCAAACGCATTTGGTCGCTATTGCGCCGCGTtggctggc                                   >  1:2512643/1‑68 (MQ=255)
                 gCAATGATGAGCGTACTTAGCGTGGTGTCGCAAACGCATTTGGTCGCTATTGCGCAGCGTtggctggc                                   >  1:3394046/1‑68 (MQ=255)
                    atgatgAGCGTACTTAGCGTGGTGTCGCAAACGCATCTGGTCGCTATTGCGCCGCGTTGGTTGGCTGaa                               <  1:2465409/69‑1 (MQ=255)
                    atgatgAGCGTACTTAGCGTGGTGTCGCAAACGCATCTGGTCGCTATTGCGCCGCGTTGGTTGGCTGaa                               <  1:2593119/69‑1 (MQ=255)
                    atgatgAGCGTACTTAGCGTGGTGTCGCAAACGCATCTGGTCGCTATTGCGCCGCGTTGGTTGGCTGaa                               <  1:2748237/69‑1 (MQ=255)
                    atgatgAGCGTACTTAGCGTGGTGTCGCAAACGCATCTGGTCGCTATTGCGCCGCGTTGGTTGGCTGaa                               <  1:3259151/69‑1 (MQ=255)
                    atgatgAGCGTACTTAGCGTGGTGTCGCAAACGCATCTGGTCGCTATTGCGCCGCGTTGGTTGGCTGaa                               <  1:529404/69‑1 (MQ=255)
                    atgatgAGCGTACTTAGCGTGGTGTCGCAAACGCATCTGGTCGCTATTGCGCCGCGTTGGTTGGCTGaa                               <  1:73571/69‑1 (MQ=255)
                    atgatgAGCGTACTTAGCGTGGTGTCGCAAACGCATCTGGTCGCTATTGCGCCGCGTTGGTTGGCTGaa                               <  1:794212/69‑1 (MQ=255)
                    atgatgAGCGTACTTAGCGTGGTGTCGCAAACGCATCTGGTCGCTATTGCGCCGCGTTGGTTGGCTGaa                               <  1:935135/69‑1 (MQ=255)
                    atgatgAGCGTACTTAGCGTGGTGTCGCAAACGCATCTGGTCGCTATTGCGCCGCGTTGGTTGGCTGaa                               <  1:2077429/69‑1 (MQ=255)
                    atgatgAGCGTACTTAGCGTGGTGTCGCAAACGCATCTGGTCGCTATTGCGCCGCGTTGGTTGGCTGaa                               <  1:1135442/69‑1 (MQ=255)
                    atgatgAGCGTACTTAGCGTGGTGTCGCAAACGCATCTGGTCGCTATTGCGCCGCGTTGGTTGGCTGaa                               <  1:1917030/69‑1 (MQ=255)
                    atgatgAGCGTACTTAGCGTGGTGTCGCAAACGCATCTGGTCGCTATTGCGCCGCGTTGGTTGGCTGaa                               <  1:1495614/69‑1 (MQ=255)
                    atgatgAGCGTACTTAGCGTGGTGTCGCAAACGCATCTGGTCGCTATTGCGCCGCGTTGGTTGGCTGaa                               <  1:1653683/69‑1 (MQ=255)
                    atgatgAGCGTACTTAGCGTGGTGTCGCAAACGCATCTGGTCGCTATTGCGCCGCGTTGGTTGGCTGaa                               <  1:2202146/69‑1 (MQ=255)
                    atgatgAGCGTACTTAGCGTGGTGTCGCAAACGCATCTGGTCGCTATCGCGCCGCGTTGATTGGCTGaa                               <  1:3270245/69‑1 (MQ=255)
                    atgatgAGCGTACTTAGCGTGGTGTCGCAAACGCATCTGGGCGCTATTGCGCCGCGTTGGTTGGCTGaa                               <  1:909805/69‑1 (MQ=255)
                     tgatgaGCGTACTTAGCGTGGTGTCGCAAACGCATTTGGTCGCTATTGCGCCGCGTTGGCTGGCTGAag                              >  1:407133/1‑69 (MQ=255)
                     tgatgaGCGTACTTAGCGTGGTGTCGCAAACGCATTTGGTCGCTATTGCGCCGCGTTGGCTGGCTGAag                              >  1:2029746/1‑69 (MQ=255)
                     tgatgaGCGTACTTAGCGTGGTGTCGCAAACGCATTTGGTCGCTATTGCGCCGCGTTGGCTGGCTGAag                              >  1:3147375/1‑69 (MQ=255)
                     tgatgaGCGTACTTAGCGTGGTGTCGCAAACGCATTTGGTCGCTATTGCGCCGCGTTGGCTGGCTGAag                              >  1:3423844/1‑69 (MQ=255)
                     tgatgaGCGTACTTAGCGTGGTGTCGCAAACGCATTTGGTCGCTATTGCGCCGCGTTGGCTGGCTGAag                              >  1:2508655/1‑69 (MQ=255)
                     tgatgaGCGTACTTAGCGTGGTGTCGCAAACGCATTTGGTCGCTATTGCGCCGCGTTGGCTGGCTGAag                              >  1:446026/1‑69 (MQ=255)
                     tgatgaGCGTACTTAGCGTGGTGTCGCAAACGCATTTGGTCGCTATTGCGCCGCGTTGGCTGGCTGAag                              >  1:535375/1‑69 (MQ=255)
                     tgatgaGCGTACTTAGCGTGGTGTCGCAAACGCATTTGGTCGCTATTGCGCCGCGTTGGCTGGCTGAag                              >  1:821557/1‑69 (MQ=255)
                     tgatgaGCGTACTTAGCGTGGTGTCGCAAACGCATTTGGTCGCTATTGCGCCGCGTTGGCTGGCTGAag                              >  1:107549/1‑69 (MQ=255)
                     tgatgaGCGTACTTAGCGTGGTGTCGCAAACGCATTTGGTCGCTATTGCGCCGCGTTGGCTGGCTGAag                              >  1:102949/1‑69 (MQ=255)
                     tgatgaGCGTACTTAGCGTGGTGTCGCAAACGCATTTGGTCGCTATTGCGCCGCGTTGGCTGGCTGAag                              >  1:2703917/1‑69 (MQ=255)
                     tgatgaGCGTACTTAGCGTGGTGTCGCAAACGCATTTGGTCGCTATTGCGCCGCGTTGGCTGGCTGAag                              >  1:2623335/1‑69 (MQ=255)
                     tgatgaGCGTACTTAGCGTGGTGTCGCAAACGCATTTGGTCGCTATTGCGCCGCGTTGGCTGGCTGAag                              >  1:2752922/1‑69 (MQ=255)
                     tgatgaGCGTACTTAGCGTGGTGTCGCAAACGCATTTGGTCGCTATTGCGCCGCGTTGGCTGGCTGAag                              >  1:2770956/1‑69 (MQ=255)
                     tgatgaGCGTACTTAGCGTGGTGTCGCAAACGCATCTGGTCGCTATTGCGCCGCGTTGGTTGGCTGaa                               <  1:2777517/68‑1 (MQ=255)
                       atgaGCGTACTTAGCGTGGTGTCGCGAACGCATTTGGTCGCTATTGCGCCGCGTTGGCTGGCTGAagag                            >  1:2265708/1‑69 (MQ=255)
                       atgaGCGTACTTAGCGTGGTGTCGCAAACGCATTTGGTCGCTATTGCGCCGCGTTGGCTGGCTGAagag                            >  1:699721/1‑69 (MQ=255)
                       atgaGCGTACTTAGCGTGGTGTCGCAAACGCATTTGGTCGCTATTGCGCCGCGTTGGCTGGCTGAagag                            >  1:581380/1‑69 (MQ=255)
                       atgaGCGTACTTAGCGTGGTGTCGCAAACGCATTTGGTCGCTATTGCGCCGCGTTGGCTGGCTGAagag                            >  1:68211/1‑69 (MQ=255)
                       atgaGCGTACTTAGCGTGGTGTCGCAAACGCATTTGGTCGCTATTGCGCCGCGTTGGCTGGCTGAagag                            >  1:726700/1‑69 (MQ=255)
                       atgaGCGTACTTAGCGTGGTGTCGCAAACGCATTTGGTCGCTATTGCGCCGCGTTGGCTGGCTGAagag                            >  1:1156468/1‑69 (MQ=255)
                       atgaGCGTACTTAGCGTGGTGTCGCAAACGCATTTGGTCGCTATTGCGCCGCGTTGGCTGGCTGAagag                            >  1:815715/1‑69 (MQ=255)
                       atgaGCGTACTTAGCGTGGTGTCGCAAACGCATTTGGTCGCTATTGCGCCGCGTTGGCTGGCTGAagag                            >  1:2790292/1‑69 (MQ=255)
                       atgaGCGTACTTAGCGTGGTGTCGCAAACGCATTTGGTCGCTATTGCGCCGCGTTGGCTGGCTGAag                              >  1:373999/1‑67 (MQ=255)
                                  tAGCGTGGTGTCGCAAACGCATTTGGTCGCTATTGCGCCGCGTTGGCTGGCTGAAGAGTTCGCTGAATc                 <  1:2799578/69‑1 (MQ=255)
                                  tAGCGTGGTGTCGCAAACGCATTTGGTCGCTATTGCGCCGCGTTGGCTGGCTGAAGAGTTCGCTGAATc                 <  1:1427469/69‑1 (MQ=255)
                                  tAGCGTGGTGTCGCAAACGCATTTGGTCGCTATTGCGCCGCGTTGGCTGGCTGAAGAGTTCGCTGAATc                 <  1:650044/69‑1 (MQ=255)
                                  tAGCGTGGTGTCGCAAACGCATTTGGTCGCTATTGCGCCGCGTTGGCTGGCTGAAGAGTTCGCTGAATc                 <  1:2587360/69‑1 (MQ=255)
                                  tAGCGTGGTGTCGCAAACGCATTTGGTCGCTATTGCGCCGCGTTGGCTGGCTGAAGAGTTCGCTGAATc                 <  1:2520164/69‑1 (MQ=255)
                                  tAGCGTGGTGTCGCAAACGCATTTGGTCGCTATTGCGCCGCGTTGGCTGGCTGAAGAGTTCGCTGAATc                 <  1:2694210/69‑1 (MQ=255)
                                  tAGCGTGGTGTCGCAAACGCATTTGGTCGCTATTGCGCCGCGTTGGCTGGCTGAAGAGTTCGCTGAATc                 <  1:1519175/69‑1 (MQ=255)
                                  tAGCGTGGTGTCGCAAACGCATTTGGTCGCTATTGCGCCGCGTTGGCTGGCTGAAGAGTTCGCTGAATc                 <  1:2307011/69‑1 (MQ=255)
                                  tAGCGTGGTGTCGCAAACGCATTTGGTCGCTATTGCGCCGCGTTGGCTGGCTGAAGAGTTCGCTGAATc                 <  1:372805/69‑1 (MQ=255)
                                  tAGCGTGGTGTCGCAAACGCATTTGGTCGCTATTGCGCCGCGTTGGCTGGCTGAAGAGTTCGCTGAATc                 <  1:15338/69‑1 (MQ=255)
                                  tAGCGTGGTGTCGCAAACGCATTTGGTCGCTATTGCGCCGCGTTGGCTGGCTGAAGAGTTCGCTGAATc                 <  1:2958834/69‑1 (MQ=255)
                                         gtgtCGCAAACGCATTTGGTCGCTATTGCGCCGCGTTGGCTGGCTGAAGAGTTCGCTGAATCCTTAGa           >  1:1476055/1‑68 (MQ=255)
                                         gtgtCGCAAACGCATTTGGTCGCTATTGCGCCGCGTTGGCTGGCTGAAGAGTTCGCTGAATCCTTAGa           >  1:3219989/1‑68 (MQ=255)
                                         gtgtCGCAAACGCATTTGGTCGCTATTGCGCCGCGTTGGCTGGCTGAAGAGTTCGCTGAATCCTTAGa           >  1:1976140/1‑68 (MQ=255)
                                         gtgtCGCAAACGCATTTGGTCGCTATTGCGCCGCGTTGGCTGGCTGAAGAGTTCGCTGAATCCTTAGa           >  1:1094479/1‑68 (MQ=255)
                                         gtgtCGCAAACGCATTTGGTCGCTATTGCGCCGCGTTGGCTGGCTGAAGAGTTAGCTGAATCCTTAGa           >  1:3187791/1‑68 (MQ=255)
                                           gtCGCAAACGCATTTGGTCGCTATTGCGCCGCGTTGGCTGGCTGAAGAGTTCGCTGAATCCTTAGAAt         <  1:3386749/68‑1 (MQ=255)
                                              gCAAACGCATTTGGTCGCTATTGCGCCGCGTTGGCTGGCTGAAGAGTTCGCTGAATCCTTAGAATTACa     >  1:41679/1‑69 (MQ=255)
                                              gCAAACGCATTTGGTCGCTATTGCGCCGCGTTGGCTGGCTGAAGAGTTCGCTGAATCCTTAGAATTACa     >  1:2127196/1‑69 (MQ=255)
                                              gCAAACGCATTTGGTCGCTATTGCGCCGCGTTGGCTGGCTGAAGAGTTCGCTGAATCCTTAGAATTACa     >  1:278793/1‑69 (MQ=255)
                                              gCAAACGCATTTGGTCGCTATTGCGCCGCGTTGGCTGGCTAAAGAGTTCGCTGAATCCTTAGAATTACa     >  1:391444/1‑69 (MQ=255)
                                                 aaCGCATTTGGTCGCTATTGCGCCGCGTTGGCTGGCTGAAGAGTTCGCTGAATCCTTAGAATTACAGGt  <  1:2389513/69‑1 (MQ=255)
                                                 aaCGCATTTGGTCGCTATTGCGCCGCGTTGGCTGGCTGAAGAGTTCGCTGAATCCTTAGAATTACAGGt  <  1:2086120/69‑1 (MQ=255)
                                                 aaCGCATTTGGTCGCTATTGCGCCGCGTTGGCTGGCTGAAGAGTTCGCTGAATCCTTAGAATTACAGGt  <  1:203403/69‑1 (MQ=255)
                                                 aaCGCATTTGGTCGCTATTGCGCCGCGTTGGCTGGCTGAAGAGTTCGCTGAATCCTTAGAATTACAGGt  <  1:1177960/69‑1 (MQ=255)
                                                 aaCGCATTTGGTCGCTATTGCGCCGCGTTGGCTGGCTGAAGAGTTCGCTGAATCCTTAGAATTACAGGt  <  1:1666789/69‑1 (MQ=255)
                                                 aaCGCATTTGGTCGCTATTGCGCCGCGTTGGCTGGCTGAAGAGTTCGCTGAATCCTTAGAATTACAGGt  <  1:330318/69‑1 (MQ=255)
                                                 aaCGCATTTGGTCGCTATTGCGCCGCGTTGGCTGGCTGAAGAGTTCGCTGAATCCTTAGAATTACAGGt  <  1:3344800/69‑1 (MQ=255)
                                                       tttGGTCGCTATTGCGCCGCGTTGGCTGGCTGAAGAGTTCGCTGAATCCTTAGAATTACAGGt  <  1:2596044/63‑1 (MQ=255)
                                                        |                                                             
TCGCGTATCAGGGCATGGCAATGATGAGCGTACTTAGCGTGGTGTCGCAAACGCATTTGGTCGCTATTGCGCCGCGTTGGCTGGCTGAAGAGTTCGCTGAATCCTTAGAATTACAGGT  >  minE/69814‑69931

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: