| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 677,923 | C→T | 71.1% | G29G (GGC→GGT) | yccR → | conserved hypothetical protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 677,923 | 0 | C | T | 71.1% | 136.8 / 64.4 | 138 | G29G (GGC→GGT) | yccR | conserved hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base C (23/17); new base T (37/61); total (60/78) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 3.89e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.63e-01 | |||||||||||
Only 100 of 142 total aligned reads displayed.
AGCGGATCTATAAATCACAAGAATACCTGGCAACGTTGGGCACAATTGAATACCGATCATTGTTTGGCAGTTACAGCCTGACCGTTGACGACACGGTGTTTGCGATGGTTTCTGATGGTGAGTTGT > minE/677856‑677981 | aGCGGATCTATAAATCACAAGAATACCTGGCAACGTTGGGCACAATTGAATACCGATCATTGTTTGGc < 1:1103519/68‑1 (MQ=255) cGGATCTATAAATCACAAGAATACCTGGCAACGTTGGGCACAATTGAATACCGATCATTGTTTGGCAGt < 1:958655/69‑1 (MQ=255) cGGATCTATAAATCACAAGAATACCTGGCAACGTTGGGCACAATTGAATACCGATCATTGTTTGGCAGt > 1:428513/1‑69 (MQ=255) cGGATCTATAAATCACAAGAATACCTGGCAACGTTGGGCACAATTGAATACCGATCATTGTTTGGCAGt > 1:2264411/1‑69 (MQ=255) cGGATCTATAAATCACAAGAATACCTGGCAACGTTGGGCACAATTGAATACCGATCATTGTTTGGCAGt > 1:1387425/1‑69 (MQ=255) taAATCACAAGAATACCTGGCAACGTTGGGCACAATTGAATACCGATCATTGTTTGGCAGTTACAGCCt > 1:1018834/1‑69 (MQ=255) taAATCACAAGAATACCTGGCAACGTTGGGCACAATTGAATACCGATCATTGTTTGGCAGTTACAGCCt > 1:1032952/1‑69 (MQ=255) taAATCACAAGAATACCTGGCAACGTTGGGCACAATTGAATACCGATCATTGTTTGGCAGTTACAGCCt > 1:3059987/1‑69 (MQ=255) aaTCACAAGAATACCTGGCAACGTTGGGCACAATTGAATACCGATCATTGTTTGGCAGTTACAGCCTg < 1:3171062/68‑1 (MQ=255) aaTCACAAGAATACCTGGCAACGTTGGGCACAATTGAATACCGATCATTGTTTGGCAGTTACAGCCTg < 1:3150081/68‑1 (MQ=255) tCACAAGAATACCTGGCAACGTTGGGCACAATTGAATACCGATCATTGTTTGGCAGTTACAGCCTGAcc < 1:3289989/69‑1 (MQ=255) tCACAAGAATACCTGGCAACGTTGGGCACAATTGAATACCGATCATTGTTTGGCAGTTACAGCCTGAcc < 1:118037/69‑1 (MQ=255) acaAGAATACCTGGCAACGTTGGGCACAATTGAATACCGATCATTGTTTGGCAGTTACAGCCTGACCg > 1:1051769/1‑68 (MQ=255) aaTACCTGGCAACGTTGGGCACAATTGAATACCGATCATTGTTTGGCAGTTACAGCCTGACCGtt < 1:2357204/65‑1 (MQ=255) tACCTGGCAACGTTGTGCACAATTGAATACCGATCATTGTTTGGTAGt > 1:1265217/1‑48 (MQ=255) tACCTGGCAACGTTGGGCACAATTGAATACCGATCATTGTTTGGTAg > 1:260982/1‑47 (MQ=255) tACCTGGCAACGTTGGGCACAATTGAATACCGATCATTGTTTGGTAGt > 1:1994249/1‑48 (MQ=255) tACCTGGCAACGTTGGGCACAATTGAATACCGATCATTGTTTGGTAGt > 1:66966/1‑48 (MQ=255) tACCTGGCAACGTTGGGCACAATTGAATACCGATCATTGTTTGGTAGt > 1:1117324/1‑48 (MQ=255) tACCTGGCAACGTTGGGCACAATTGAATACCGATCATTGTTTGGTAGt > 1:136114/1‑48 (MQ=255) tACCTGGCAACGTTGGGCACAATTGAATACCGATCATTGTTTGGTAGt > 1:3109745/1‑48 (MQ=255) tACCTGGCAACGTTGGGCACAATTGAATACCGATCATTGTTTGGTAGt > 1:554363/1‑48 (MQ=255) tACCTGGCAACGTTGGGCACAATTGAATACCGATCATTGTTTGGTAGt > 1:502709/1‑48 (MQ=255) tACCTGGCAACGTTGGGCACAATTGAATACCGATCATTGTTTGGTAGt > 1:298588/1‑48 (MQ=255) tACCTGGCAACGTTGGGCACAATTGAATACCGATCATTGTTTGGTAGt > 1:454690/1‑48 (MQ=255) tACCTGGCAACGTTGGGCACAATTGAATACCGATCATTGTTTGGTAGt > 1:1641520/1‑48 (MQ=255) tACCTGGCAACGTTGGGCACAATTGAATACCGATCATTGTTTGGTAGt > 1:1677310/1‑48 (MQ=255) tACCTGGCAACGTTGGGCACAATTGAATACCGATCATTGTTTGGTAGt > 1:374926/1‑48 (MQ=255) tACCTGGCAACGTTGGGCACAATTGAATACCGATCATTGTTTGGTAGt > 1:3300515/1‑48 (MQ=255) tACCTGGCAACGTTGGGCACAATTGAATACCGATCATTGTTTGGTAGt > 1:3183158/1‑48 (MQ=255) tACCTGGCAACGTTGGGCACAATTGAATACCGATCATTGTTTGGTAGt > 1:1058385/1‑48 (MQ=255) tACCTGGCAACGTTGGGCACAATTGAATACCGATCATTGTTTGGCAGTTACAGCCTGACCGTTgacgac < 1:2580633/69‑1 (MQ=255) aCCTGGCAACGTTGGGCACAATTGAATACCGATCATTGTTTGGc < 1:3083217/44‑1 (MQ=255) ccTGGCAACGTTGGGCACAATTGAATACCGATCATTGTTTGGCAGTTACAGCCTGACCGTTGACGacac > 1:2209405/1‑69 (MQ=255) ccTGGCAACGTTGGGCACAATTGAATACCGATCATTGTTTGGCAGTTACAGCCTGACCGTTGACGacac > 1:2562261/1‑69 (MQ=255) ccTGGCAACGTTGGGCACAATTGAATACCGATCATTGTTTGGCAGTTACAGCCTGACCGTTGACGacac > 1:1235563/1‑69 (MQ=255) catGGCAACGTTGGGCACAATTGAATACCGATCATTGTTTGGCAGTTACAGCCTGACCGTTGACGacac > 1:2826625/3‑69 (MQ=255) gCAACGTTGGGCACAATTGAATACCGATCATTGTTTGGTAGTTACAGCCTAACCGTTGACGACACGgtg > 1:471221/1‑69 (MQ=255) gCAACGTTGGGCACAATTGAATACCGATCATTGTTTGGTAGTTACAGCCTAACCGTTGACGACACGgtg > 1:2139204/1‑69 (MQ=255) gCAACGTTGGGCACAATTGAATACCGATCATTGTTTGGTAGTTACAGCCTAACCGTTGACGACACGgtg > 1:289027/1‑69 (MQ=255) gCAACGTTGGGCACAATTGAATACCGATCATTGTTTGGTAGTTACAGCCTAACCGTTGACGACACGgtg > 1:615272/1‑69 (MQ=255) gCAACGTTGGGCACAATTGAATACCGATCATTGTTTGGTAGTTACAGCCTAACCGTTGACGACACGgtg > 1:1173402/1‑69 (MQ=255) gCAACGTTGGGCACAATTGAATACCGATCATTGTTTGGTAGTTACAGCCTAACCGTTGACGACACGgtg > 1:1351293/1‑69 (MQ=255) gCAACGTTGGGCACAATTGAATACCGATCATTGTTTGGTAGTTACAGCCTAACCGTTGACGACACGgtg > 1:243331/1‑69 (MQ=255) gCAACGTTGGGCACAATTGAATACCGATCATTGTTTGGTAGTTACAGCCTAACCGTTGACGACACGgtg > 1:2463603/1‑69 (MQ=255) gggCACAATTGAATACCGATCATTGTTTGGCAGTTACAGCCTGACCGTTGACGACACGGTGTTTGCGAt < 1:2820844/69‑1 (MQ=255) gggCACAATTGAATACCGATCATTGTTTGGCAGTTACAGCCTGACCGTTGACGACACGGTGTTTGCGAt < 1:1247838/69‑1 (MQ=255) gggCACAATTGAATACCGATCATTGTTTGGCAGTTACAGCCTGACCGTTGACGACACGGTGTTTGCGAt < 1:2207475/69‑1 (MQ=255) gggAACAATTGAATACCGATCATTGTTTGGCAGTTACAGCCTGACCGTTGACGACACGGTGTTTGCGAt > 1:3135723/1‑69 (MQ=255) gAATACCGATCATTGTTTGGTAGTTACAGCCTAACCGTTg < 1:1617559/40‑1 (MQ=255) gAATACCGATCATTGTTTGGTAGTTACAGCCTAACCGTTg < 1:2042723/40‑1 (MQ=255) gAATACCGATCATTGTTTGGTAGTTACAGCCTAACCGTTg < 1:3067511/40‑1 (MQ=255) gAATACCGATCATTGTTTGGTAGTTACAGCCTAACCGTTg < 1:2496590/40‑1 (MQ=255) gAATACCGATCATTGTTTGGTAGTTACAGCCTAACCGTTg < 1:1051599/40‑1 (MQ=255) gAATACCGATCATTGTTTGGTAGTTACAGCCTAACCGTTg < 1:2104386/40‑1 (MQ=255) gAATACCGATCATTGTTTGGTAGTTACAGCCTAACCGTTg < 1:3425544/40‑1 (MQ=255) gAATACCGATCATTGTTTGGTAGTTACAGCCTAACCGTTg < 1:373572/40‑1 (MQ=255) gAATACCGATCATTGTTTGGTAGTTACAGCCTAACCGTTg < 1:502511/40‑1 (MQ=255) gAATACCGATCATTGTTTGGTAGTTACAGCCTAACCGTTg < 1:1189086/40‑1 (MQ=255) gAATACCGATCATTGTTTGGTAGTTACAGCCTAACCGTTg < 1:1214382/40‑1 (MQ=255) gAATACCGATCATTGTTTGGTAGTTACAGCCTAACCGTTg < 1:751498/40‑1 (MQ=255) gAATACCGATCATTGTTTGGTAGTTACAGCCTAACCGTTg < 1:2613474/40‑1 (MQ=255) gAATACCGATCATTGTTTGGTAGTTACAGCCTAACCGTTg < 1:91986/40‑1 (MQ=255) gAATACCGATCATTGTTTGGTAGTTACAGCCTAACCGTTg < 1:92382/40‑1 (MQ=255) gAATACCGATCATTGTTTGGTAGTTACAGCCTAACCGTTg < 1:1017327/40‑1 (MQ=255) gAATACCGATCATTGTTTGGCAGTTACAGCCTGACCGTTGACGACACGGTGTTTGCGATGGTTTCTGa > 1:836989/1‑68 (MQ=255) gAATACCGATCATTGTTTGGCAGTTACAGCCTGACCGTTGACGACACGGTGTTTGCGATGGTTTCTGa > 1:3244962/1‑68 (MQ=255) gAATACCGATCATTGTTTGGCAGTTACAGCCTGACCGTTGACGACACGGTGTTTGCGATGGTTTCTGAt > 1:1855575/1‑69 (MQ=255) cATTGTTTGGTAGTTACAGCCTAACCGTTGACGACACGGTGTTTGCGATGGTTTCTGATGGTGAGTTGt < 1:2483708/69‑1 (MQ=255) cATTGTTTGGTAGTTACAGCCTAACCGTTGACGACACGGTGTTTGCGATGGTTTCTGATGGTGAGTTGt < 1:2696808/69‑1 (MQ=255) cATTGTTTGGTAGTTACAGCCTAACCGTTGACGACACGGTGTTTGCGATGGTTTCTGATGGTGAGTTGt < 1:804239/69‑1 (MQ=255) cATTGTTTGGTAGTTACAGCCTAACCGTTGACGACACGGTGTTTGCGATGGTTTCTGATGGTGAGTTGt < 1:2614079/69‑1 (MQ=255) cATTGTTTGGTAGTTACAGCCTAACCGTTGACGACACGGTGTTTGCGATGGTTTCTGATGGTGAGTTGt < 1:3426578/69‑1 (MQ=255) cATTGTTTGGTAGTTACAGCCTAACCGTTGACGACACGGTGTTTGCGATGGTTTCTGATGGTGAGTTGt < 1:3368549/69‑1 (MQ=255) cATTGTTTGGTAGTTACAGCCTAACCGTTGACGACACGGTGTTTGCGATGGTTTCTGATGGTGAGTTGt < 1:3334038/69‑1 (MQ=255) cATTGTTTGGTAGTTACAGCCTAACCGTTGACGACACGGTGTTTGCGATGGTTTCTGATGGTGAGTTGt < 1:1846952/69‑1 (MQ=255) cATTGTTTGGTAGTTACAGCCTAACCGTTGACGACACGGTGTTTGCGATGGTTTCTGATGGTGAGTTGt < 1:2422850/69‑1 (MQ=255) cATTGTTTGGTAGTTACAGCCTAACCGTTGACGACACGGTGTTTGCGATGGTTTCTGATGGTGAGTTGt < 1:941724/69‑1 (MQ=255) cATTGTTTGGTAGTTACAGCCTAACCGTTGACGACACGGTGTTTGCGATGGTTTCTGATGGTGAGTTGt < 1:1074588/69‑1 (MQ=255) cATTGTTTGGTAGTTACAGCCTAACCGTTGACGACACGATGTTTGCGATGGTTTCTGATGGTGAGTTGt < 1:3123514/69‑1 (MQ=255) cATTGTTTGGCAGTTACAGCCTGACCGTTGACGACACGGTGTTTGCGATGGTTTCTGATGGTGAGTTGt < 1:1979010/69‑1 (MQ=255) cATTGTTTGGCAGTTACAGCCTGACCGTTGACGACACGGTGTTTGCGATGGTTTCTGATGGTGAGTTGt < 1:1685152/69‑1 (MQ=255) cATTGTTTGGCAGTTACAGCCTGACCGTTGACGACACGGTGTTTGCGATGGTTTCTGATGGTGAGTTGt < 1:1038007/69‑1 (MQ=255) cATTGTTTGGCAGTTACAGCCTGACCGTTGACGACACGGTGTTTGCGATGGTTTCTGATGGTGAGTTGt < 1:2623667/69‑1 (MQ=255) aTTGTTTGGTAGTTACAGCCTAACCGTTGACGACACGGTGTTTGCGATGGTTTCTGATGGTGAGTTGt < 1:3017154/68‑1 (MQ=255) aTTGTTTGGTAGTTACAGCCTAACCGTTGACGACACGGTGTTTGCGATGGTTTCTGATGGTGAGTTGt < 1:1000490/68‑1 (MQ=255) ttGGTAGTTACAGCCTAACCGTTGACGACACGGTGTTTGCGATgg < 1:910043/45‑1 (MQ=255) ttGGTAGTTACAGCCTAACCGTTGACGACACGGTGTTTGCGATgg < 1:2629729/45‑1 (MQ=255) ttGGTAGTTACAGCCTAACCGTTGACGACACGGTGTTTGCGATgg < 1:1377331/45‑1 (MQ=255) ttGGTAGTTACAGCCTAACCGTTGACGACACGGTGTTTGCGATgg < 1:226217/45‑1 (MQ=255) ttGGTAGTTACAGCCTAACCGTTGACGACACGGTGTTTGCGATgg < 1:518325/45‑1 (MQ=255) ttGGTAGTTACAGCCTAACCGTTGACGACACGGTGTTTGCGATgg < 1:1389736/45‑1 (MQ=255) ttGGTAGTTACAGCCTAACCGTTGACGACACGGTGTTTGCGATgg < 1:2381290/45‑1 (MQ=255) ttGGTAGTTACAGCCTAACCGTTGACGACACGGTGTTTGCGATgg < 1:1559086/45‑1 (MQ=255) ttGGTAGTTACAGCCTAACCGTTGACGACACGGTGTTTGCGATgg < 1:2932008/45‑1 (MQ=255) ttGGTAGTTACAGCCTAACCGTTGACGACACGGTGTTTGCGATgg < 1:2981321/45‑1 (MQ=255) ttGGTAGTTACAGCCTAACCGTTGACGACACGGTGTTTGCGATgg < 1:1075867/45‑1 (MQ=255) ttGGTAGTTACAGCCTAACCGTTGACGACACGGTGTTTGCGATgg < 1:3061720/45‑1 (MQ=255) ttGGTAGTTACAGCCTAACCGTTGACGACACGGTGTTTGCGATgg < 1:3180586/45‑1 (MQ=255) ttGGTAGTTACAGCCTAACCGTTGACGACACGGTGTTTGCGATgg < 1:1985638/45‑1 (MQ=255) | AGCGGATCTATAAATCACAAGAATACCTGGCAACGTTGGGCACAATTGAATACCGATCATTGTTTGGCAGTTACAGCCTGACCGTTGACGACACGGTGTTTGCGATGGTTTCTGATGGTGAGTTGT > minE/677856‑677981 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |