| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 678,513 | T→C | 37.2% | G690G (GGA→GGG) | yccS ← | predicted inner membrane protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 678,513 | 0 | T | C | 37.2% | 41.8 / 49.4 | 79 | G690G (GGA→GGG) | yccS | predicted inner membrane protein |
| Reads supporting (aligned to +/- strand): ref base T (29/20); new base C (19/10); total (49/30) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.36e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.85e-01 | |||||||||||
TCCTGCGGAACTTGAGTAATCTGGCGTTGCAGACGACCAATCTCAGGCAGTAATGCAATCAATAATCCAACTTGTTGTACGACCAGAGGTTCTTTGCTGTCTGCCCGTGGTTCAAGTTGCTGCATCCGCTGT > minE/678448‑678579 | tCCTGCGGATCTTGAGTAATCTGGCGTTGCAGACGACCAATCTCAGGCAGTAATGCAATCAATAATCCa > 1:2299899/1‑69 (MQ=255) gAACTTGAGTAATCTGGCGTTGCAGACGACCAATCTCAGGCAGTAATGCAATCAATAATCCAACttgt < 1:948448/68‑1 (MQ=255) gAACTTGAGTAATCTGGCGTTGCAGACGACCAATCTCAGGCAGTAATGCAATCAATAATCCAACttgt < 1:2947189/68‑1 (MQ=255) gAACTTGAGTAATCTGGCGTTGCAGACGACCAATCTCAGGCAGTAATGCAATCAATAATCCAACttgt < 1:3431384/68‑1 (MQ=255) gAACTTGAGTAATCTGGCGTTGCAGACGACCAATCTCAGGCAGTAATGCAATCAATAATCCAACttgt < 1:700866/68‑1 (MQ=255) aaCTTGAGTAATCTGGCGTTGCAGACGACCAATCTCAGGCAGTAATGCAATCAATAATCCAACttgttg < 1:1532085/69‑1 (MQ=255) aaCTTGAGTAATCTGGCGTTGCAGACGACCAATCTCAGGCAGTAATGCAATCAATAATCCAACttgttg < 1:1294899/69‑1 (MQ=255) cTTGAGTAATCTGGCGTTGCAGACGACCAATCTCAGGCAGTAATGCAATCAATAATCCAACTtgttgt > 1:3227481/1‑68 (MQ=255) cTTGAGTAATCTGGCGTTGCAGACGACCAATCTCAGGCAGTAATGCAATCAATAATCCAACTtgttgt > 1:260686/1‑68 (MQ=255) cTTGAGTAATCTGGCGTTGCAGACGACCAATCTCAGGCAGTAATGCAATCAATAATCCAACTtgttgt > 1:1409475/1‑68 (MQ=255) cTTGAGTAATCTGGCGTTGCAGACGACCAATCTCAGGCAGTAATGCAATCAATAATCCAACTtgttgt > 1:738033/1‑68 (MQ=255) aTCTGGCGTTGCAGACGACCAATCTCAGGCAGTAATGCAATCAATAATCCAACTTGTTGTACGACCaga < 1:2509438/69‑1 (MQ=255) aTCTGGCGTTGCAGACGACCAATCTCAGGCAGTAATGCAATCAATAATCCAACTTGTTGTACGACCaga < 1:2271099/69‑1 (MQ=255) aTCTGGCGTTGCAGACGACCAATCTCAGGCAGTAATGCAATCAATAATCCAACTTGTTGTACGACCaga < 1:2233264/69‑1 (MQ=255) aTCTGGCGTTGCAGACGACCAATCTCAGGCAGTAATGCAATCAATAATCCAACTTGTTGTACGACCaga < 1:2146946/69‑1 (MQ=255) aTCTGGCGTTGCAGACGACCAATCTCAGGCAGTAATGCAATCAATAATCCAACTTGTTGTACGACCaga < 1:2609410/69‑1 (MQ=255) aTCTGGCGTTGCAGACGACCAATCTCAGGCAGTAATGCAATCAATAATCCAACTTGTTGTACGACCaga < 1:1168724/69‑1 (MQ=255) aTCTGGCGTTGCAGACGACCAATCTCAGGCAGTAATGCAATCAATAATCCAACTTGTTGTACGACCaga < 1:2953670/69‑1 (MQ=255) aTCTGGCGTTGCAGACGACCAATCTCAGGCAGTAATGCAATCAATAATCCAACTTGTTGTACGACCaga < 1:3297022/69‑1 (MQ=255) tcAGGCAGTAATGCAATCAATAATCCAACTTGTTGTACGACCAGAGGTTCTTTGCTGTCTGCCCGTgg > 1:1959416/1‑68 (MQ=255) tcAGGCAGTAATGCAATCAATAATCCAACTTGTTGTACGACCAGAGGTTCTTTGCTGTCTGCCCGTgg > 1:2415816/1‑68 (MQ=255) tcAGGCAGTAATGCAATCAATAATCCAACTTGTTGTACGACCAGAGGTTCTTTGCTGTCTGCCCGTgg > 1:2378210/1‑68 (MQ=255) tcAGGCAGTAATGCAATCAATAATCCAACTTGTTGTACGACCAGAGGTTCTTTGCTGTCTGCCCGTgg > 1:837338/1‑68 (MQ=255) tcAGGCAGTAATGCAATCAATAATCCAACTTGTTGTACGACCAGAGGTTCTTTGCTGTCTGCCCGTgg > 1:3013150/1‑68 (MQ=255) tcAGGCAGTAATGCAATCAATAATCCAACTTGTTGTACGACCAGAGGTTCTTTGCTGTCTGCCCGTgg > 1:1885354/1‑68 (MQ=255) tcAGGCAGTAATGCAATCAATAATCCAACTTGTTGTACGACCAGAGGTTCTTTGCTGTCTGCCCGTgg > 1:1495224/1‑68 (MQ=255) tcAGGCAGTAATGCAATCAATAATCCAACTTGTTGTACGACCAGAGGTTCTTTGCTGTCTGCCCGTgg > 1:1242581/1‑68 (MQ=255) tcAGGCAGTAATGCAATCAATAATCCAACTTGTTGTACGACCAGAGGTTCTTTGCTGTCTGCCCGTg > 1:1918120/1‑67 (MQ=255) tcAGGCAGTAATGCAATCAATAATCCAACTTGTTGTACGACCAGAGGTTCTTTGCTGTCTGCCCGTg > 1:1002014/1‑67 (MQ=255) aTGCAATTAATAACCCAACTTGTTGTACGACCAGAGGTTCTTTGCTGTCTGCCCGTGGTTCAAGTtgc > 1:1385647/1‑68 (MQ=255) aTGCAATCAATAACCCAACTTGTTGTACGACCAGAGGTTCTTTg > 1:583593/1‑44 (MQ=255) aTGCAATCAATAACCCAACTTGTTGTACGACCAGAGGTTCTTTGCTGTCTGCCCGTGGTTCAAGTtgc > 1:1382009/1‑68 (MQ=255) aTGCAATCAATAACCCAACTTGTTGTACGACCAGAGGTTCTTTGCTGTCTGCCCGTGGTTCAAGTtgc > 1:3195174/1‑68 (MQ=255) aTGCAATCAATAACCCAACTTGTTGTACGACCAGAGGTTCTTTGCTGTCTGCCCGTGGTTCAAGTtgc > 1:130514/1‑68 (MQ=255) aTGCAATCAATAACCCAACTTGTTGTACGACCAGAGGTTCTTTGCTGTCTGCCCGTGGTTCAAGTtgc > 1:2436311/1‑68 (MQ=255) aTGCAATCAATAACCCAACTTGTTGTACGACCAGAGGTTCTTTGCTGTCTGCCCGTGGTTCAAGTtgc > 1:897292/1‑68 (MQ=255) aTGCAATCAATAACCCAACTTGTTGTACGACCAGAGGTTCTTTGCTGTCTGCCCGTGGTTCAAGTtgc > 1:1496259/1‑68 (MQ=255) aTGCAATCAATAACCCAACTTGTTGTACGACCAGAGGTTCTTTGCTGTCTGCCCGTGGTTCAAGTtgc > 1:807595/1‑68 (MQ=255) aTGCAATCAATAACCCAACTTGTTGTACGACCAGAGGTTCTTTGCTGTCTGCCCGTGGTTCAAGTtgc > 1:2547310/1‑68 (MQ=255) aTGCAATCAATAACCCAACTTGTTGTACGACCAGAGGTTCTTTGCTGTCTGCCCGTGGTTCAAGTtgc > 1:2198428/1‑68 (MQ=255) aTGCAATCAATAACCCAACTTGTTGTACGACCAGAGGTTCTTTGCTGTCTGCCCGTGGTTCAAGTtgc > 1:2590186/1‑68 (MQ=255) aTGCAATCAATAACCCAACTTGTTGTACGACCAGAGGTTCTTTGCTGTCTGCCCGTGGTTCAAGTtgc > 1:2118335/1‑68 (MQ=255) aTGCAATCAATAACCCAACTTGTTGTACGACCAGAGGTTCTTTGCTGTCTGCCCGTGGTTCAAGTtgc > 1:2104978/1‑68 (MQ=255) aTGCAATCAATAACCCAACTTGTTGTACGACCAGAGGTTCTTTGCTGTCTGCCCGTGGTTCAAGTtgc > 1:292317/1‑68 (MQ=255) aTGCAATCAATAACCCAACTTGTTGTACGACCAGAGGTTCTTTGCTGTCTGCCCGTGGTTCAAGTtgc > 1:1617820/1‑68 (MQ=255) aTGCAATCAATAACCCAACTTGTTGTACGACCAGAGGTTCTTTGCTGTCTGCCCGTGGTTCAAGTtgc > 1:3341383/1‑68 (MQ=255) aTGCAATCAATAACCCAACTTGTTGTACGACCAGAGGTTCTTTGCTGTCTGCCCGTGGTTCAAGTtgc > 1:1800497/1‑68 (MQ=255) aTGCAATCAATAACCCAACTTGTTGTACGACCAGAGGTTCTTTGCTGTCTGACCGTGGTTCAAGTtgc > 1:3412270/1‑68 (MQ=255) aTGCAATCAATAAACCAACTTGTTGTACGACCAGAGGt > 1:2318533/1‑38 (MQ=255) gCAATCAATAACCCAACTTGTTGTACGACCAGAGGTTCTTTGCTGTCTGCCCGTGGTTCAAGTtgcttc < 1:2409196/69‑3 (MQ=255) gCAATCAATAACCCAACTTGTTGTACGACCAGAGGTTCTTTGCTGTCTGCCCGTGGTTCAAGTtgctgc < 1:637018/69‑1 (MQ=255) gCAATCAATAACCCAACTTGTTGTACGACCAGAGGTTCTTTGCTGTCTGCCCGTGGTTCAAGTtgctgc < 1:385678/69‑1 (MQ=255) gCAATCAATAACCCAACTTGTTGTACGACCAGAGGTTCTTTGCTGTCTGCCCGTGGTTCAAGTtgctgc < 1:1825080/69‑1 (MQ=255) gCAATCAATAACCCAACTTGTTGTACGACCAGAGGTTCTTTGCTGTCTGCCCGTGGTTCAAGTtgctgc < 1:190680/69‑1 (MQ=255) gCAATCAATAACCCAACTTGTTGTACGACCAGAGGTTCTTTGCTGTCTGCCCGTGGTTCAAGTtgctgc < 1:2965401/69‑1 (MQ=255) gCAATCAATAACCCAACTTGTTGTACGACCAGAGGTTCTTTGCTGTCTGCCCGTGGTTCAAGTtgctgc < 1:2682481/69‑1 (MQ=255) gCAATCAATAACCCAACTTGTTGTACGACCAGAGGTTCTTTGCTGTCTGCCCGTGGTTCAAGTtgctgc < 1:2542428/69‑1 (MQ=255) gCAATCAATAACCCAACTTGTTGTACGACCAGAGGTTCTTTGCTGTCTGCCCGTGGTTCAAGTtgctgc < 1:228258/69‑1 (MQ=255) caatcaatAATCCAACTTGTTGTACGACCAGAGGTTCTTTGCTGTCTGCCCGTGGTTCAAGTTGCTGCa < 1:2226062/69‑1 (MQ=255) caatcaatAATCCAACTTGTTGTACGACCAGAGGTTCTTTGCTGTCTGCCCGTGGTTCAAGTTGCTGCa > 1:1588031/1‑69 (MQ=255) caatcaatAATCCAACTTGTTGTACGACCAGAGGTTCTTTGCTGTCTGCCCGTGGTTCAAGTTGCTGCa > 1:1392381/1‑69 (MQ=255) caatcaatAATCCAACTTGTTGTACGACCAGAGGTTCTTTGCTGTCTGCCCGTGGTTCAAGTTGCTGCa > 1:784269/1‑69 (MQ=255) caatcaatAATCCAACTTGTTGTACGACCAGAGGTTCTTTGCTGTCTGCCCGTGGTTCAAGTTGCTGCa < 1:78788/69‑1 (MQ=255) caatcaatAATCCAACTTGTTGTACGACCAGAGGTTCTTTGCTGTCTGCCCGTGGTTCAAGTTGCTGCa < 1:1840948/69‑1 (MQ=255) caatcaatAATCCAACTTGTTGTACGACCAGAGGTTCTTTGCTGTCTGCCCGTGGTTCAAGTTGCTGCa > 1:2529492/1‑69 (MQ=255) caatcaatAATCCAACTTGTTGTACGACCAGAGGTTCTTTGCTGTCTGCCCGTGGTTCAAGTTGCTGCa < 1:2788764/69‑1 (MQ=255) caatcaatAATCCAACTTGTTGTACGACCAGAGGTTCTTTGCTGTCTGCCCGTGGTTCAAGTTGCTGCa > 1:2051502/1‑69 (MQ=255) caatcaatAATCCAACTTGTTGTACGACCAGAGGTTCTTTGCTGTCTGCCCGTGGTTCAAGTTGCTGCa < 1:2155975/69‑1 (MQ=255) caatcaatAATCCAACTTGTTGTACGACCAGAGGTTCTTTGCTGTCTGCCCGTGGTTCAAGTTGCTGCa < 1:1073426/69‑1 (MQ=255) caatcaatAACCCAACTTGTTGTACGACCAGAGGTTCTTTGCTGTCTGCCCGTGGTTCAAGTtgctgc < 1:230982/68‑1 (MQ=255) atcaatAATCCAACTTGTTGTACGACCAGAGGTTCTTTg > 1:2857384/1‑39 (MQ=255) atcaatAATCCAACTTGTTGTACGACCAGAGGTTCTTTGCTGt > 1:2195395/1‑43 (MQ=255) atcaatAATCCAACTTGTTGTACGACCAGAGGTTCTTTGCTGTCTGCCCGTGGTTCAAGTTGCTGCAt > 1:1553228/1‑68 (MQ=255) atcaatAATCCAACTTGTTGTACGACCAGAGGTTCTTTGCTGTCTGCCCGTGGTTCAAGTTGCTGCATc > 1:1987540/1‑69 (MQ=255) atcaatAATCCAACTTGTTGTACGACCAGAGGTTCTTTGCTGTCTGCCCGTGGTTCAAGTTGCTGCATc > 1:2535962/1‑69 (MQ=255) atcaatAATCCAACTTGTTGTACGACCAGAGGTTCTTTGCTGTCTGCCCGTGGTTCAAGTTGCTGCATc > 1:2641921/1‑69 (MQ=255) atcaatAATCCAACTTGTTGTACGACCAGAGGTTCTTTGCTGTCTGCCCGTGGTTCAAGTTGCTGCATc > 1:2454416/1‑69 (MQ=255) atcaatAATCCAACTTGTTGTACGACCAGAGGTTCTTTGCTGTCTGCCCGTGGTTCAAGTTGCTGCATc > 1:3253224/1‑69 (MQ=255) atcaatAATCCAACTTGTTGTACGACCAGAGGTTCTTTGCTGTCTGCCCGTGGTTCAAGTTGCTGCATc > 1:68301/1‑69 (MQ=255) aataatCCAACTTGTTGTACGACCAGTGGTTCTTTGCTGTCTGCCCGTGGTTCAAGTTGCTGCATCCGc < 1:1946195/69‑1 (MQ=255) aataatCCAACTTGTTGTACGACCAGAGGTTCTTTGCTGTCTGCCCGTGGTTCAAGTTGCTGGATCCGc < 1:1161306/69‑1 (MQ=255) aataatCCAACTTGTTGTACGACCAGAGGTTCTTTGCTGTCTGCCCGTGGTTCAAGTTGCTGCATCCGc < 1:1422774/69‑1 (MQ=255) aataatCCAACTTGTTGTACGACCAGAGGTTCTTTGCTGTCTGCCCGTGGTTCAAGTTGCTGCATCCGc < 1:242724/69‑1 (MQ=255) aataatCCAACTTGTTGTACGACCAGAGGTTCTTTGCTGTCTGCCCGTGGTTCAAGTTGCTGCATCCGc < 1:1727218/69‑1 (MQ=255) aataatCCAACTTGTTGTACGACCAGAGGTTCTTTGCTGTCTGCCCGTGGTTCAAGTTGCTGCATCCGc < 1:3301999/69‑1 (MQ=255) aataatCCAACTTGTTGTACGACCAGAGGTTCTTTGCTGTCTGCCCGTGGTTCAAGTTGCTGCATCCGc < 1:1746722/69‑1 (MQ=255) aataatCCAACTTGTTGTACGACCAGAGGTTCTTTGCTGTCTGCCCGTGGTTCAAGTTGCTGCATCCGc > 1:256519/1‑69 (MQ=255) aataatCCAACTTGTTGTACGACCAGAGGTTCTTTGCTATCTGCCCGTGGTTCAAGTTGCTGCATCCGc < 1:1061093/69‑1 (MQ=255) aataacCCAACTTGTTGTACGACCAGAGGTTCTTTGCTGTCTGCCCGTGGTTCAAGTtgctgc < 1:1576475/63‑1 (MQ=255) atCCAACTTGTTGTACGACCAGAGGTTCTTTGCTGTCTGCCCGTGGTTCAAGTTGCTGCATCCGCTGt > 1:3308097/1‑68 (MQ=255) atCCAACTTGTTGTACGACCAGAGGTTCTTTGCTGTCTGCCCGTGGTTCAAGTTGCTGCATCCGCTGt > 1:1247035/1‑68 (MQ=255) atCCAACTTGTTGTACGACCAGAGGTTCTTTGCTCTCTGCCCGTGGTTCAAGTTGCTGCATCCGCTGt > 1:766233/1‑68 (MQ=255) atCCAACTTGTTGCACGACCAGAGGTTCTTTGCTGTCTGCCCGTGGTTCAAGTTGCTGCATCCGCTGt > 1:2014754/1‑68 (MQ=255) | TCCTGCGGAACTTGAGTAATCTGGCGTTGCAGACGACCAATCTCAGGCAGTAATGCAATCAATAATCCAACTTGTTGTACGACCAGAGGTTCTTTGCTGTCTGCCCGTGGTTCAAGTTGCTGCATCCGCTGT > minE/678448‑678579 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |