| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 680,715 | G→A | 30.0% | G111G (GGC→GGT) | yccF ← | conserved inner membrane protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 680,715 | 0 | G | A | 30.0% | 71.5 / 31.6 | 73 | G111G (GGC→GGT) | yccF | conserved inner membrane protein |
| Reads supporting (aligned to +/- strand): ref base G (16/35); new base A (2/20); total (18/55) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 7.33e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.39e-01 | |||||||||||
TCTACCGATACCACGCGACGACCAACCGGCCATAGTGCAATAGCGGCAATTTTAAAGTTCGCAATGCCGACAGGAATGCCAATGATTGAAATACATTGTGCGATGCCCGTTGCAATGTGCATCAGGCAT > minE/680650‑680778 | tCTACCGATACCACGCGACGACCAACCGGCCATAGTGCAATAGCGGCAATTTTAAAGTTCGCAATGcc > 1:2357115/1‑68 (MQ=255) gATACCACGCGACGACCAACCGGCCATAGTGCAATAGCGGCAATTTTAAAGTTCGCAATGCCGACAgg < 1:2617217/68‑1 (MQ=255) gATACCACGCGACGACCAACCGGCCATAGTGCAATAGCGGCAATTTTAAAGTTCGCAATGCCGACAgg < 1:2463033/68‑1 (MQ=255) ccACGCGACGACCAACCGGCCATAGTGCAATAGCGGCGATTTTAAAGTTCGCAATGCCGACAgg < 1:2965381/64‑1 (MQ=255) ccACGCGACGACCAACCGGCCATAGTGCAATAGCGGCAATTTTAAAGTTCGCAATGCCGACAgg < 1:1306566/64‑1 (MQ=255) ccACGCGACGACCAACCGGCCATAGTGCAATAGCGGCAATTTTAAAGTTCGCAATGCCGACAgg < 1:2612564/64‑1 (MQ=255) ccACGCGACGACCAACCGGCCATAGTGCAATAGCGGCAATTTTAAAGTTCGCAATGCCGACAgg < 1:1367840/64‑1 (MQ=255) ccACGCGACGACCAACCGGCCATAGTGCAATAGCGGCAATTTTAAAGTTCGCAATGCCGACAgg < 1:158723/64‑1 (MQ=255) ccACGCGACGACCAACCGGCCATAGTCCAATAGCGGCAATTTTAAAGTTCGCAATGCCGACAgg < 1:1250746/64‑1 (MQ=255) aCGCGACGACCAACCGGCCATAGTGCAATAGCGGCAATTTTAAAGTTCGCAATGCCGACAGGAATGCCa < 1:974698/69‑1 (MQ=255) aCGCGACGACCAACCGGCCATAGTGCAATAGCGGCAATTTTAAAGTTCGCAATGCCGACAGGAATGCCa < 1:71579/69‑1 (MQ=255) aCGCGACGACCAACCGGCCATAGTGCAATAGCGGCAATTTTAAAGTTCGCAATGCCGACAGGAATGCCa < 1:2672217/69‑1 (MQ=255) aCGCGACGACCAACCGGCCATAGTGCAATAGCGGCAATTTTAAAGTTCGCAATGCCGACAGGAATGCCa < 1:2319079/69‑1 (MQ=255) aCGCGACGACCAACCGGCCATAGTGCAATAGCGGCAATTTTAAAGTTCGCAATGCCGACAGGAATGCCa < 1:1968240/69‑1 (MQ=255) aCGCGACGACCAACCGGCCATAGTGCAATAGCGGCAATTTTAAAGTTCGCAATGCCGACAGGAATGCCa < 1:189884/69‑1 (MQ=255) aCGCGACGACCAACCGGCCATAGTGCAATAGCGGCAATTTTAAAGTTCGCAATGCCGACAGGAATGCCa < 1:1821078/69‑1 (MQ=255) gcgACGACCGACCGGGCATAGTGCAATAGCGGCAATTTTAAAGTTCGCAATACCGACAGGAATGCCAAt < 1:2648430/69‑1 (MQ=255) gcgACGACCGACCGGCCATAGTGCAGTAGCGGCAATTTTAAAGTTCGCAATACCGACAGGAATGCCAAt < 1:2831184/69‑1 (MQ=255) gcgACGACCGACCGGCCATAGTGCAATAGCGGCAATTTTAAAGTTCGCAATACCGACAGGAATGCCAAt < 1:233199/69‑1 (MQ=255) gcgACGACCGACCGGCCATAGTGCAATAGCGGCAATTTTAAAGTTCGCAATACCGACAGGAATGCCAAt > 1:2730403/1‑69 (MQ=255) gcgACGACCGACCGGCCATAGTGCAATAGCGGCAATTTTAAAGTTCGCAATACCGACAGGAATGCCAAt < 1:2776128/69‑1 (MQ=255) gcgACGACCGACCGGCCATAGTGCAATAGCGGCAATTTTAAAGTTCGCAATACCGACAGGAATGCCAAt < 1:2696012/69‑1 (MQ=255) gcgACGACCGACCGGCCATAGTGCAATAGCGGCAATTTTAAAGTTCGCAATACCGACAGGAATGCCAAt < 1:2650209/69‑1 (MQ=255) gcgACGACCGACCGGCCATAGTGCAATAGCGGCAATTTTAAAGTTCGCAATACCGACAGGAATGCCAAt < 1:2841425/69‑1 (MQ=255) gcgACGACCGACCGGCCATAGTGCAATAGCGGCAATTTTAAAGTTCGCAATACCGACAGGAATGCCAAt < 1:2603918/69‑1 (MQ=255) gcgACGACCGACCGGCCATAGTGCAATAGCGGCAATTTTAAAGTTCGCAATACCGACAGGAATGCCAAt < 1:2598150/69‑1 (MQ=255) gcgACGACCGACCGGCCATAGTGCAATAGCGGCAATTTTAAAGTTCGCAATACCGACAGGAATGCCAAt < 1:2529724/69‑1 (MQ=255) gcgACGACCGACCGGCCATAGTGCAATAGCGGCAATTTTAAAGTTCGCAATACCGACAGGAATGCCAAt < 1:304952/69‑1 (MQ=255) gcgACGACCGACCGGCCATAGTGCAATAGCGGCAATTTTAAAGTTCGCAATACCGACAGGAATGCCAAt < 1:237531/69‑1 (MQ=255) gcgACGACCGACCGGCCATAGTGCAATAGCGGCAATTTTAAAGTTCGCAATACCGACAGGAATGCCAAt > 1:1410835/1‑69 (MQ=255) gcgACGACCGACCGGCCATAGTGCAATAGCGGCAATTTTAAAGTTCGCAATACCGACAGGAATGCCAAt < 1:843413/69‑1 (MQ=255) gcgACGACCGACCGGCCATAGTGCAATAGCGGCAATTTTAAAGTTCGCAATACCGACAGGAATGCCAAt < 1:1694984/69‑1 (MQ=255) gcgACGACCGACCGGCCATAGTGCAATAGCGGCAATTTTAAAGTTCGCAATACCGACAGGAATGCCAAt < 1:1662434/69‑1 (MQ=255) gcgACGACCGACCGGCCATAGTGCAATAGCGGCAATTTTAAAGTTCGCAATACCGACAGGAATGCCAAt < 1:3434385/69‑1 (MQ=255) gcgACGACCGACCGGCCATAGTGCAATAGCGGCAATTTTAAAGTTCGCAATACCGACAGGAATGCCAAt < 1:2321559/69‑1 (MQ=255) gcgACGACCGACCGGCCATAGTGCAATAGCGGCAATTTTAAAGTTCGCAATACCGACAGGAATGCCAAt < 1:1479843/69‑1 (MQ=255) cgacgaCCGACCGGCCATAGTGCAATAGCGGCAATTTTAAAGTTCGCAATACCGACAGGAATGCCAAt < 1:685974/68‑1 (MQ=255) gTGCAATAGCGGCAATTTTAAAGTTCTCAATGCCGACAGGAATGCCAATg < 1:2789679/50‑1 (MQ=255) gTGCAATAGCGGCAATTTTAAAGTTCGCAATGCCGACa > 1:34358/1‑38 (MQ=255) gTGCAATAGCGGCAATTTTAAAGTTCGCAATGCCGACAGGa > 1:398893/1‑41 (MQ=255) gTGCAATAGCGGCAATTTTAAAGTTCGCAATGCCGACAGGAATGCCAATg > 1:2999018/1‑50 (MQ=255) gTGCAATAGCGGCAATTTTAAAGTTCGCAATGCCGACAGGAATGCCAATg > 1:3204939/1‑50 (MQ=255) gTGCAATAGCGGCAATTTTAAAGTTCGCAATGCCGACAGGAATGCCAATg > 1:3119399/1‑50 (MQ=255) gTGCAATAGCGGCAATTTTAAAGTTCGCAATGCCGACAGGAATGCCAATg > 1:3359016/1‑50 (MQ=255) gTGCAATAGCGGCAATTTTAAAGTTCGCAATGCCGACAGGAATGCCAATg > 1:3418203/1‑50 (MQ=255) gTGCAATAGCGGCAATTTTAAAGTTCGCAATGCCGACAGGAATGCCAATg < 1:3069680/50‑1 (MQ=255) gTGCAATAGCGGCAATTTTAAAGTTCGCAATGCCGACAGGAATGCCAATg > 1:563111/1‑50 (MQ=255) gTGCAATAGCGGCAATTTTAAAGTTCGCAATGCCGACAGGAATGCCAATg > 1:1232425/1‑50 (MQ=255) gTGCAATAGCGGCAATTTTAAAGTTCGCAATGCCGACAGGAATGCCAATg > 1:806809/1‑50 (MQ=255) gTGCAATAGCGGCAATTTTAAAGTTCGCAATGCCGACAGGAATGCCAATg > 1:1785591/1‑50 (MQ=255) gTGCAATAGCGGCAATTTTAAAGTTCGCAATGCCGACAGGAATGCCAATg < 1:1633446/50‑1 (MQ=255) gTGCAATAGCGGCAATTTTAAAGTTCGCAATGCCGACAGGAATGCCAATg < 1:161173/50‑1 (MQ=255) gTGCAATAGCGGCAATTTTAAAGTTCGCAATGCCGACAGGAATGCCAATg < 1:2167186/50‑1 (MQ=255) gTGCAATAGCGGCAATTTTAAAGTTCGCAATGCCGACAGGAATGCCAATg > 1:136486/1‑50 (MQ=255) gTGCAATAGCGGCAATTTTAAAGTTCGCAATGCCGACAGGAATGCCAATg < 1:2497187/50‑1 (MQ=255) gTGCAATAGCGGCAATTTTAAAGTTCGCAATGCCGACAGGAATGCCAATg > 1:887261/1‑50 (MQ=255) gTGCAATAGCGGCAATTTTAAAGTTCGCAATGCCGACAGGAATGCCAATg > 1:1313910/1‑50 (MQ=255) gTGCAATAGCGGCAATTTTAAAGTTCGCAATGCCGACAGGAATGCCAATg > 1:1165662/1‑50 (MQ=255) tGCAATAGCGGCAATTTTAAAGTTCGCAATACCGACAGGAATGCCAAt < 1:1788915/48‑1 (MQ=255) gCAATAGCGGCAATTTTAAAGTTCGCAATGCCGACAGGAATGCCAATGATTGAAATACATTGTGCGATg < 1:1908646/69‑1 (MQ=255) gCAATTTTAAAGTTCGCAATGCCGACAGGAATGCCa < 1:2308322/36‑1 (MQ=255) aGTTCGCAATGCCGACAGGAATGCCAATGATTGAAATACATTGTGCGATGCCCGTTGCAATGTGCATc < 1:1994316/68‑1 (MQ=255) aGTTCGCAATGCCGACAGGAATGCCAATGATTGAAATACATTGTGCGATGCCCGTTGCAATGTGCATc < 1:3418014/68‑1 (MQ=255) gTTCGCAATGCCGACAGGAATGCCAATGATTGAAATACATTGTGCGATGCCCGTTGCAATGTGCATCa < 1:743249/68‑1 (MQ=255) gTTCGCAATGCCGACAGGAATGCCAATGATTGAAATACATTGTGCGATGCCCGTTGCAATGTGCATCa < 1:989511/68‑1 (MQ=255) gTTCGCAATGCCGACAGGAATGCCAATGATTGAAATACATTGTGCGATGCCCGTTGCAATGTGCATCa < 1:3323706/68‑1 (MQ=255) gTTCGCAATGCCGACAGGAATGCCAATGATTGAAATACATTGTGCGATGCCCGTTGCAATGTGCATCa < 1:1581368/68‑1 (MQ=255) gTTCGCAATGCCGACAGGAATGCCAATGATTGAAATACATTGTGCGATGCCCGTTGCAATGTGCATCa < 1:133115/68‑1 (MQ=255) gTTCGCAATGCCGACAGGAATGCCAATGATTGAAATACATTGTGCGATGCCCGTTGCAATGTGCATCa < 1:1141806/68‑1 (MQ=255) ttCGCAATGCCGACAGGAATGCGAATGATTGAAATACATTGTGCGATGCCCGTTGCAATGTGCATCAgg < 1:2129513/69‑1 (MQ=255) ttCGCAATGCCGACAGGAATGCCAATGATTGAAATACATTGTGCGATGCCCGTTGCAATGTGCATCAgg < 1:1784326/69‑1 (MQ=255) ttCGCAATGCCGACAGGAATGCCAATGATTGAAATACATTGTGCGATGCCCGTTGCAATGTGCATCAgg < 1:105670/69‑1 (MQ=255) aaTGCCGACAGGAATGCCAATGATTGAAATACATTGTGCGATGCCCGTTGCAATGTGCATCAGGCAt < 1:2727075/67‑1 (MQ=255) | TCTACCGATACCACGCGACGACCAACCGGCCATAGTGCAATAGCGGCAATTTTAAAGTTCGCAATGCCGACAGGAATGCCAATGATTGAAATACATTGTGCGATGCCCGTTGCAATGTGCATCAGGCAT > minE/680650‑680778 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |