| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 10,173 | C→T | 62.8% | V108I (GTA→ATA) | yaaH ← | conserved inner membrane protein associated with acetate transport |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 10,173 | 0 | C | T | 62.8% | 30.0 / 36.0 | 59 | V108I (GTA→ATA) | yaaH | conserved inner membrane protein associated with acetate transport |
| Reads supporting (aligned to +/- strand): ref base C (16/6); new base T (25/12); total (41/18) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 7.75e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
GAAAACGAATTGCAGAACGCGTGCGCCTTTCAGCGTGCCGAAGAACATAAACAGCGTAAATACGCCCCACAGACCCAGGTAGACACCAAGGAACTGTGCATTTGGCGCATCGGTCAGACCCAGTTTC > minE/10111‑10237 | gAAAACGAATTGCAGAACGCGTGCGCCTTTCAGCGTGCCGAAGAACATAAACAGCGTAAATACGccc < 1:1107016/67‑1 (MQ=255) tCAGCGTGCCGAAGAACATAAACAGCGTAAATATGc > 1:3159935/1‑36 (MQ=255) tCAGCGTGCCGAAGAACATAAACAGCGTAAATATGCCCcaca > 1:1022340/1‑42 (MQ=255) tCAGCGTGCCGAAGAACATAAACAGCGTAAATATGCCCcaca > 1:996881/1‑42 (MQ=255) tCAGCGTGCCGAAGAACATAAACAGCGTAAATATGCCCcaca > 1:716791/1‑42 (MQ=255) tCAGCGTGCCGAAGAACATAAACAGCGTAAATATGCCCcaca > 1:697834/1‑42 (MQ=255) tCAGCGTGCCGAAGAACATAAACAGCGTAAATATGCCCcaca > 1:400655/1‑42 (MQ=255) tCAGCGTGCCGAAGAACATAAACAGCGTAAATATGCCCcaca > 1:371084/1‑42 (MQ=255) tCAGCGTGCCGAAGAACATAAACAGCGTAAATATGCCCcaca > 1:2896434/1‑42 (MQ=255) tCAGCGTGCCGAAGAACATAAACAGCGTAAATATGCCCcaca > 1:287016/1‑42 (MQ=255) tCAGCGTGCCGAAGAACATAAACAGCGTAAATATGCCCcaca > 1:2512604/1‑42 (MQ=255) tCAGCGTGCCGAAGAACATAAACAGCGTAAATATGCCCcaca > 1:2431423/1‑42 (MQ=255) tCAGCGTGCCGAAGAACATAAACAGCGTAAATATGCCCcaca > 1:2381442/1‑42 (MQ=255) tCAGCGTGCCGAAGAACATAAACAGCGTAAATATGCCCcaca > 1:2354664/1‑42 (MQ=255) tCAGCGTGCCGAAGAACATAAACAGCGTAAATATGCCCcaca > 1:230538/1‑42 (MQ=255) tCAGCGTGCCGAAGAACATAAACAGCGTAAATATGCCCcaca > 1:1854823/1‑42 (MQ=255) tCAGCGTGCCGAAGAACATAAACAGCGTAAATATGCCCcaca > 1:1265770/1‑42 (MQ=255) tCAGCGTGCCGAAGAACATAAACAGCGTAAATATGCCCcaca > 1:1131765/1‑42 (MQ=255) tCAGCGTGCCGAAGAACATAAACAGCGTAAATATGCCCcaca > 1:1696296/1‑42 (MQ=255) tCAGCGTGCCGAAGAACATAAACAGCGTAAATATGCCCca > 1:2519463/1‑40 (MQ=255) tCAGCGTGCCGAAGAACATAAACAGCGTAAATATGCCCCACAGACCCAGGTAGACACCAAGGAACtgtg > 1:1826372/1‑69 (MQ=255) tCAGCGTGCCGAAGAACATAAACAGCGTAAATATGCCCCACAGACCCAGGTAGACACCAAGGAACtgtg > 1:2932861/1‑69 (MQ=255) tCAGCGTGCCGAAGAACATAAACAGCGTAAATATGCCCCACAGACCCAGGTAGACACCAAGGAACtgtg > 1:2970159/1‑69 (MQ=255) tCAGCGTGCCGAAGAACATAAACAGCGTAAATATGCCCCACAGACCCAGGTAGACACCAAGGAACtgtg > 1:524670/1‑69 (MQ=255) tCAGCGTGCCGAAGAACATAAACAGCGTAAATATGCCCCACAGACCCAGGTAGACACCAAGGAACtgtg > 1:883331/1‑69 (MQ=255) tCAGCGTGCCGAAGAACATAAACAGCGTAAATATGCCCCACAGACCCAGGTAGACACCAAGGAACtgtg > 1:2093726/1‑69 (MQ=255) cAGCGTGCCTAAGAACATAAACAGCGTAAATACGCCCCACAGCCCCAGGTa > 1:248283/1‑51 (MQ=39) cAGCGTGCCGAAGAACATAAACAGCGTAAATACGCCCCACAGACCCAGGTAGACACCAAGGAACTGTGc > 1:1054963/1‑69 (MQ=255) cAGCGTGCCGAAGAACATAAACAGCGTAAATACGCCCCACAGACCCAGGTAGACACCAAGGAACTGTGc > 1:1050950/1‑69 (MQ=255) cAGCGTGCCGAAGAACATAAACAGCGTAAATACGCCCCACAGACCCAGGTAGACACCAAGGAACTGTGc > 1:738552/1‑69 (MQ=255) cAGCGTGCCGAAGAACATAAACAGCGTAAATACGCCCCACAGACCCAGGTAGACACCAAGGAACTGTGc > 1:1187677/1‑69 (MQ=255) cAGCGTGCCGAAGAACATAAACAGCGTAAATACGCCCCACAGACCCAGGTAGACACCAAGGAACTGTGc > 1:2760571/1‑69 (MQ=255) cAGCGTGCCGAAGAACATAAACAGCGTAAATACGCCCCACAGACCCAGGTAGACACCAAGGAACTGTGc > 1:2770324/1‑69 (MQ=255) gCGTGCCGAAGAACATAAACAGCGTAAATACGCCCCACAGACCCAGGTAGACACCAAGGAACTGTGCa < 1:1914279/68‑1 (MQ=255) tGCCGAAGAACATAAACAGCGTAAATCTGCCCCACAGACCCAGGTAGACACCAAGGAACTGTGCATTTg < 1:2194833/69‑1 (MQ=255) tGCCGAAGAACATAAACAGCGTAAATATGCCCCACAGACCCAGGTAGACACCAAGGAACTGTGCATTTg < 1:588004/69‑1 (MQ=255) tGCCGAAGAACATAAACAGCGTAAATATGCCCCACAGACCCAGGTAGACACCAAGGAACTGTGCATTTg < 1:2843564/69‑1 (MQ=255) tGCCGAAGAACATAAACAGCGTAAATATGCCCCACAGACCCAGGTAGACACCAAGGAACTGTGCATTTg < 1:2906336/69‑1 (MQ=255) tGCCGAAGAACATAAACAGCGTAAATATGCCCCACAGACCCAGGTAGACACCAAGGAACTGTGCATTTg < 1:1363072/69‑1 (MQ=255) tGCCGAAGAACATAAACAGCGTAAATATGCCCCACAGACCCAGGTAGACACCAAGGAACTGTGCATTTg < 1:1854639/69‑1 (MQ=255) tGCCGAAGAACATAAACAGCGTAAATATGCCCCACAGACCCAGGTAGACACCAAGGAACTGTGCATTTg < 1:2204124/69‑1 (MQ=255) tGCCGAAGAACATAAACAGCGTAAATATGCCCCACAGACCCAGGTAGACACCAAGGAACTGTGCATTTg < 1:2689221/69‑1 (MQ=255) tGCCGAAGAACATAAACAGCGTAAATATGCCCCACAGACCCAGGTAGACACCAAGGAACTGTGCATTTg < 1:2300181/69‑1 (MQ=255) tGCCGAAGAACATAAACAGCGTAAATATGCCCCACAGACCCAGGTAGACACCAAGGAACTGTGCATTTg < 1:176482/69‑1 (MQ=255) tGCCGAAGAACATAAACAGCGTAAATATGCCCCACAGACCCAGGTAGACACCAAGGAACTGTGCATTTg < 1:1400429/69‑1 (MQ=255) ccGAAGAACATAAACAGCGTAAATACGCCCCACAGACCCAGGTAGACACCAAGGAACTGTGCATTTgg > 1:1308750/1‑68 (MQ=255) ccGAAGAACATAAACAGCGTAAATACGCCCCACAGACCCAGGTAGACACCAAGGAACTGTGCATTTgg > 1:3141064/1‑68 (MQ=255) ccGAAGAACATAAACAGCGTAAATACGCCCCACAGACCCAGGTAGACACCAAGGAACTGTGCATTTgg > 1:2642267/1‑68 (MQ=255) ccGAAGAACATAAACAGCGTAAATACGCCCCACAGACCCAGGTAGACACCAAGGAACTGTGCATTTgg > 1:24701/1‑68 (MQ=255) ccGAAGAACATAAACAGCGTAAATACGCCCCACAGACCCAGGTAGACACAAAGGAACTGTGCa > 1:680969/1‑63 (MQ=255) aagaaCATAAACAGCGTAAATATGCCCCACAGACCCAGGTAGACACCAAGGAACTGTGCATTTg < 1:2881305/64‑1 (MQ=255) aagaaCATAAACAGCGTAAATACGCCCCACAGACCCAGGTAGACACCAAGGAACTGTGCATTTGGCGCa < 1:138647/69‑1 (MQ=255) aagaaCATAAACAGCGTAAATACGCCCCACAGACCCAGGTAGACACCAAGGAACTGTGCATTTGGCGCa < 1:3347993/69‑1 (MQ=255) aagaaCATAAACAGCGTAAATACGCCCCACAGACCCAGGTAGACACCAAGGAACTGTGCATTTGGCGCa < 1:2117607/69‑1 (MQ=255) aagaaCATAAACAGCGTAAATACGCCCCACAGACCCAGGTAGACACCAAGGAACTGTGCAGTTGGCGCa < 1:100621/69‑1 (MQ=255) aaTACGCCCCACAGACCCAGGTAGACACCAAGGAACTGTGCATTTGGCGCATCGGTCAGACCCAGTTTc > 1:1604328/1‑69 (MQ=255) aaTACGCCCCACAGACCCAGGTAGACACCAAGGAACTGTGCATTTGGCGCATCGGTCAGACCCAGTTTc > 1:627085/1‑69 (MQ=255) aaTACGCCCCACAGACCCAGGTAGACACCAAGGAACTGTGCATTTGGCGCATCGGTCAGACCCAGTTTc > 1:408221/1‑69 (MQ=255) aaTACGCCCCACAGACCCAGGTAGACACCAAGGAACTGTGCATTTGGCGCATCGGTCAGACCCAGTTTc > 1:2762827/1‑69 (MQ=255) | GAAAACGAATTGCAGAACGCGTGCGCCTTTCAGCGTGCCGAAGAACATAAACAGCGTAAATACGCCCCACAGACCCAGGTAGACACCAAGGAACTGTGCATTTGGCGCATCGGTCAGACCCAGTTTC > minE/10111‑10237 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |