| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 71,761 | C→T | 38.3% | Y464Y (TAC→TAT) | ilvI → | acetolactate synthase III, large subunit |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 71,761 | 0 | C | T | 38.3% | 63.8 / 72.8 | 112 | Y464Y (TAC→TAT) | ilvI | acetolactate synthase III, large subunit |
| Reads supporting (aligned to +/- strand): ref base C (28/41); new base T (36/7); total (64/48) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 5.74e-06 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.98e-01 | |||||||||||
Only 100 of 123 total aligned reads displayed.
CTGGCGACGGCAGTATTCAGATGAACATCCAGGAACTGTCTACCGCGTTGCAATACGAGTTGCCCGTAC‑‑‑TGGTGGTGAATCTCAATAACCGCTATCTGGGGATGGTGAAGCAGTGGCAGGACAT > minE/71706‑71829 | cTGGTGACGGCAGTATTCAGATGAACATCCAGGAACTGTCTACCGCGTTGCAATATGAg > 1:3280460/1‑59 (MQ=255)cTGGTGACGGCAGTATTCAGATGAACATCCAGGAACTGTCTACCGCGTTGCAATATGAGTTGCCCGTa > 1:185823/1‑68 (MQ=255)cTGGTGACGGCAGTATTCAGATGAACATCCAGGAACTGTCTACCGCGTTGCAATATGAGTTGCCCGTAc > 1:3087202/1‑69 (MQ=255)cTGGTGACGGCAGTATTCAGATGAACATCCAGGAACTGTCTACCGCGTTGCAATATGAGTTGCCCGTAc > 1:2896858/1‑69 (MQ=255)cTGGTGACGGCAGTATTCAGATGAACATCCAGGAACTGTCTACCGCGTTGCAATATGAGTTGCCCGTAc > 1:16977/1‑69 (MQ=255)cTGGTGACGGCAGTATTCAGATGAACATCCAGGAACTGTCTACCGCGTTGCAATATGAGTTGCCCGTAc > 1:2810919/1‑69 (MQ=255)cTGGTGACGGCAGTATTCAGATGAACATCCAGGAACTGTCTACCGCGTTGCAATATGAGTTGCCCGTAc > 1:1500477/1‑69 (MQ=255)cTGGTGACGGCAGTATTCAGATGAACATCCAGGAACTGTCTACCGCGTTGCAATATGAGTTGCCCGTAc > 1:302231/1‑69 (MQ=255)cTGGTGACGGCAGTATTCAGATGAACATCCAGGAACTGTCTACCGCGTTGCAATATGAGTTGCCCGTAc > 1:946466/1‑69 (MQ=255)cTGGTGACGGCAGTATTCAGATGAACATCCAGGAACTGTCTACCGCGTTGCAATATGAGTTGCCCGTAc > 1:758527/1‑69 (MQ=255)cTGGTGACGGCAGTATTCAGATGAACATCCAGGAACTGTCTACCGCGTTGCAATATGAGTTGCCCGTAc > 1:3056473/1‑69 (MQ=255)cTGGTGACGGCAGTATTCAGATGAACATCCAGGAACTGTCTACCGCGTTGCAATATGAGTTGCCCGTAc > 1:3148803/1‑69 (MQ=255)cTGGTGACGGCAGTATTCAGATGAACATCCAGGAACTGTCTACCGCGTTGCAATATGAGTTGCCCGTAc > 1:3174793/1‑69 (MQ=255)cTGGTGACGGCAGTATTCAGATGAACATCCAGGAACTGTCTACCGCGTTGCAATATGAGTTGCCCGTAc > 1:2313615/1‑69 (MQ=255)cTGGTGACGGCAGTATTCAGATGAACATCCAGGAACTGTCTACCGCGTTGCAATATGAGTTGCCCGTAc > 1:2224492/1‑69 (MQ=255) ggCGACGGCAGTATTCAGATGAACATCCAGGAACTGTCTACCGCGTTGCAATACGAGTTGCCCGTAC‑‑‑tg > 1:2450144/1‑69 (MQ=255) ggCGACGGCAGTATTCAGATGAACATCCAGGAACTGTCTACCGCGTTGCAATACGAGTTGCCCGTAC‑‑‑tg > 1:766012/1‑69 (MQ=255) ggCGACGGCAGTATTCAGATGAACATCCAGGAACTGTCTACCGCGTTGCAATACGAGTTGCCCGTAC‑‑‑tg > 1:1836989/1‑69 (MQ=255) ggCGACGGCAGTATTCAGATGAACATCCAGGAACTGTCTACCGCGTTGCAATACGAGTTGCCCGTAC‑‑‑tg > 1:2225555/1‑69 (MQ=255) ggCGACGGCAGTATTCAGATGAACATCCAGGAACTGTCTACCGCGTTGCAATACGAGTTGCCCGTAC‑‑‑tg > 1:153238/1‑69 (MQ=255) ggCGACGGCAGTATTCAGATGAACATCCAGGAACTGTCTACCGCGTTGCAATACGAGTTGCCCGTAC‑‑‑tg > 1:1139174/1‑69 (MQ=255) cGGCAGTATTCAGATGAACAT‑CAGGAACTGTCTACCGCGTTGCAATACGAGTTGCCCGTAC‑‑‑TGgtggtg < 1:1409215/69‑1 (MQ=255) ggCAGTATTCAGATGAACATCCAGGAACTGTCTACCGCGTTGCAATACGAGTTGCCCGTAC‑‑‑TGgtggtg < 1:1106353/69‑1 (MQ=255) ggCAGTATTCAGATGAACATCCAGGAACTGTCTACCGCGTTGCAATACGAGTTGCCCGTAC‑‑‑TGgtggtg < 1:3224453/69‑1 (MQ=255) tATTCAGATGAACATCCAGGAACTGTCTACCGCGTTGCAATATGAGTTGCCCGTAC‑‑‑TGGTGGTGAAtc > 1:741719/1‑68 (MQ=255) ttCAGATGAACATCCAGGAACTGTCTACCGCGTTGCAATACGAGTTGCCCGTAC‑‑‑TGGTGGTGAAtctc > 1:2544500/1‑68 (MQ=255) cAGATGAACATCCAGGAACTGTCTACCGCGTTGCAATACGAGTTGCCCg < 1:2236845/49‑1 (MQ=255) cAGATGAACATCCAGGAACTGTCTACCGCGTTGCAATACGAGTTGCCCg < 1:3143862/49‑1 (MQ=255) cAGATGAACATCCAGGAACTGTCTACCGCGTTGCAATACGAGTTGCCCg < 1:2439652/49‑1 (MQ=255) cAGATGAACATCCAGGAACTGTCTACCGCGTTGCAATACGAGTTGCCCGTAC‑‑‑TGGTGGTGAATCTCAAt > 1:529359/1‑69 (MQ=255) cAGATGAACATCCAGGAACTGTCTACCGCGTTGCAATACGAGTTGCCCGTAC‑‑‑TGGTGGTGAATCTCAAt > 1:600828/1‑69 (MQ=255) cAGATGAACATCCAGGAACTGTCTACCGCGTTGCAATACGAGTTGCCCGTAC‑‑‑TGGTGGTGAATCTCAAt > 1:2663652/1‑69 (MQ=255) gATGAACATCCAGGAACTGTCTACCGCGTTGCAATACGAGTTGCCCGTAC‑‑‑TGGTGGTGAATCTCAATa < 1:3030791/68‑1 (MQ=255) gATGAACATCCAGGAACTGTCTACCGCGTTGCAATACGAGTTGCCCGTAC‑‑‑TGGTGGTGAATCTCAATa < 1:656385/68‑1 (MQ=255) gATGAACATCCAGGAACTGTCTACCGCGTTGCAATACGAGTTGCCCGTAC‑‑‑TGGTGGTGAATCTCAATa < 1:3303713/68‑1 (MQ=255) gATGAACATCCAGGAACTGTCTACCGCGTTGCAATACGAGTTGCCCGTAC‑‑‑TGGTGGTGAATCTCAATa < 1:3387874/68‑1 (MQ=255) gATGAACATCCAGGAACTGTCTACCGCGTTGCAATACGAGTTGCCCGTAC‑‑‑TGGTGGTGAATCTCAATa < 1:732523/68‑1 (MQ=255) gATGAACATCCAGGAACTGTCTACCGCGTTGCAATACGAGTTGCCCGTAC‑‑‑TGGTGGTGAATCTCAATa < 1:1363957/68‑1 (MQ=255) gATGAACATCCAGGAACTGTCTACCGCGTTGCAATACGAGTTGCCCGTAC‑‑‑TGGTGGTGAATCTCAATa < 1:139091/68‑1 (MQ=255) gATGAACATCCAGGAACTGTCTACCGCGTTGCAATACGAGTTGCCCGTAC‑‑‑TGGTGGTGAATCTCAATa < 1:174106/68‑1 (MQ=255) tGAACATCCAGGAACTGTCTACCGCGTTGCAATACGAGTTGCCCGTAC‑‑‑TGGTGGTGAATCTCAATAAcc < 1:488959/69‑1 (MQ=255) tGAACATCCAGGAACTGTCTACCGCGTTGCAATACGAGTTGCCCGTAC‑‑‑TGGTGGTGAATCTCAATAAcc < 1:1184229/69‑1 (MQ=255) tGAACATCCAGGAACTGTCTACCGCGTTGCAATACGAGTTGCCCGTAC‑‑‑TGGTGGTGAATCTCAATAAcc < 1:1235324/69‑1 (MQ=255) tGAACATCCAGGAACTGTCTACCGCGTTGCAATACGAGTTGCCCGTAC‑‑‑TGGTGGTGAATCTCAATAAcc < 1:3104006/69‑1 (MQ=255) gAACATCCAGGAACTGTCTACCGCGTTGCAATATGAGTTGCCCGTAC‑‑‑TGGTGGTGAATCTCAATAAcc > 1:1816237/1‑68 (MQ=255) gAACATCCAGGAACTGTCTACCGCGTTGCAATATGAGTTGCCCGTAC‑‑‑TGGTGGTGAATCTCAATAAcc > 1:2405405/1‑68 (MQ=255) gAACATCCAGGAACTGTCTACCGCGTTGCAATATGAGTTGCCCGTAC‑‑‑TGGTGGTGAATCTCAATAAcc > 1:3140405/1‑68 (MQ=255) gAACATCCAGGAACTGTCTACCGCGTTGCAATATGAGTTGCCCGTAC‑‑‑TGGTGGTGAATCTCAATAAcc > 1:2155668/1‑68 (MQ=255) gAACATCCAGGAACTGTCTACCGCGTTGCAATATGAGTTGCCCGTAC‑‑‑TGGTGGTGAATCTCAATAAcc > 1:499108/1‑68 (MQ=255) gAACATCCAGGAACTGTCTACCGCGTTGCAATATGAGTTGCCCGTAC‑‑‑TGGTGGTGAATCTCAATAAcc > 1:96924/1‑68 (MQ=255) gAACATCCAGGAACTGTCTACCGCGTTGCAATATGAGTTGCCCGTAC‑‑‑TGGTGGTGAATCTCAATAAcc > 1:3164738/1‑68 (MQ=255) gAACATCCAGGAACTGTCTACCGCGTTGCAATATGAGTTGCCCGTAC‑‑‑TGGTGGTGAATCTCAATAAcc > 1:2894093/1‑68 (MQ=255) gAACATCCAGGAACTGTCTACCGCGTTGCAATATGAGTTGCCCGTAC‑‑‑TGGTGGTGAATCTCAATAAcc > 1:1822434/1‑68 (MQ=255) gAACATCCAGGAACTGTCTACCGCGTTGCAATATGAGTTGCCCGTAC‑‑‑TGGTGGTGAATCTCAATAAcc > 1:2808615/1‑68 (MQ=255) gAACATCCAGGAACTGTCTACCGCGTTGCAATATGAGTTGCCCGTAC‑‑‑TGGTGGTGAATCTCAATAAcc > 1:1889505/1‑68 (MQ=255) gAACATCCAGGAACTGTCTACCGCGTTGCAATATGAGTTGCCCGTAC‑‑‑TGGTGGTGAATCTCAATAAcc > 1:277010/1‑68 (MQ=255) gAACATCCAGGAACTGTCTACCGCGTTGCAATATGAGTTGCCCGTAC‑‑‑TGGTGGTGAATCTCAATAAcc > 1:1133843/1‑68 (MQ=255) gAACATCCAGGAACTGTCTACCGCGTTGCAATATGAGTTGCCCGTAC‑‑‑TGGTGGTGAATCTCAATAAcc > 1:2619808/1‑68 (MQ=255) gAACATCCAGGAACTGTCTACCGCGTTGCAATATGAGTTGCCCGTAC‑‑‑TGGTGGTGAATCTCAATAAcc > 1:2573589/1‑68 (MQ=255) gAACATCCAGGAACTGTCTACCGCGTTGCAATACGAGTTGCCCGTAC‑‑‑TGGTGGTGAATCTCAATAAcc > 1:3040914/1‑68 (MQ=255) gAACATCCAGGAACTGTCTACCGCGTTGCAATACGAGTTGCCCGTAC‑‑‑TGGTGGTGAATCTCAATAAcc > 1:1821875/1‑68 (MQ=255) gAACATCCAGGAACTGTCTACCGCGTTGCAATACGAGTTGCCCGTAC‑‑‑TGGTGGTGAATCTCAATAAcc > 1:1479730/1‑68 (MQ=255) aCATCCAGGAACTGTCTACCGCGTTGCAATACGAGTTGCCCGTAC‑‑‑TGgtggtg < 1:1297887/53‑1 (MQ=255) tCCAGGAACTGTCTACCGCGTTGCAATATGAGTTGCCCGTAc < 1:244941/42‑1 (MQ=255) tCCAGGAACTGTCTACCGCGTTGCAATATGAGTTGCCCGTAc < 1:380469/42‑1 (MQ=255) tCCAGGAACTGTCTACCGCGTTGCAATATGAGTTGCCCGTAc < 1:409714/42‑1 (MQ=255) tCCAGGAACTGTCTACCGCGTTGCAATATGAGTTGCCCGTAc < 1:316587/42‑1 (MQ=255) tCCAGGAACTGTCTACCGCGTTGCAATATGAGTTGCCCGTAc < 1:595158/42‑1 (MQ=255) tCCAGGAACTGTCTACCGCGTTGCAATATGAGTTGCCCGTAc < 1:2003899/42‑1 (MQ=255) cAGGAACTGTCTACCGCGTTGCAATACGAGTTGCCCGTAC‑‑‑tggt > 1:1406339/1‑44 (MQ=255) cAGGAACTGTCTACCGCGTTGCAATACGAGTTGCCCGTAC‑‑‑TGGTGGTGAATCTCAATAACCGCTATc > 1:1041914/1‑67 (MQ=255) cAGGAACTGTCTACCGCGTTGCAATACGAGTTGCCCGTAC‑‑‑TGGTGGTGAATCTCAATAACCGCTATCTg > 1:38322/1‑69 (MQ=255) cAGGAACTGTCTACCGCGTTGCAATACGAGTTGCCCGTAC‑‑‑TGGTGGTGAATCTCAATAACCGCTATCTg > 1:1793657/1‑69 (MQ=255) cAGGAACTGTCTACCGCGTTGCAATACGAGTTGCCCGTAC‑‑‑TGGTGGTGAATCTCAATAACCGCTATCTg > 1:3250084/1‑69 (MQ=255) cAGGAACTGTCTACCGCGTTGCAATACGAGTTGCCCGTAC‑‑‑TGGTGGTGAATCTCAATAACCGCTATCTg < 1:1642195/69‑1 (MQ=255) cAGGAACTGTCTACCGCGTTGCAATACGAGTTGCCCGTAC‑‑‑TGGTGGTGAATCTCAATAACCGCTATCTg > 1:1551316/1‑69 (MQ=255) cAGGAACTGTCTACCGCGTTGCAATACGAGTTGCCAGTAC‑‑‑TGGTGGTGAATCTCAATAACCGCTATCTg > 1:1870954/1‑69 (MQ=255) ggAACTGTCTACCGCGTTGCAATACGAGTTGCCCGTAC‑‑‑TGGTGGTGAATCTCAATAACCGCTATCTggg < 1:1095633/69‑1 (MQ=255) ggAACTGTCTACCGCGTTGCAATACGAGTTGCCCGTAC‑‑‑TGGTGGTGAATCTCAATAACCGCTATCTggg < 1:1609406/69‑1 (MQ=255) ggAACTGTCTACCGCGTTGCAATACGAGTTGCCCGTAC‑‑‑TGGTGGTGAATCTCAATAACCGCTATCTggg < 1:2294436/69‑1 (MQ=255) ggAACTGTCTACCGCGTTGCAATACGAGTTGCCCGTAC‑‑‑TGGTGGTGAATCTCAATAACCGCTATCTggg < 1:2104665/69‑1 (MQ=255) ggAACTGTCTACCGCGTTGCAATACGAGTTGCCCGTAC‑‑‑TGGTGCTGAATCTCAATAACCGCTATCTggg < 1:1423295/69‑1 (MQ=255) gAACTGTCTACCGCGTTGCAATACGAGTTGCCCGTAC‑‑‑TGGTGGTGAATCTCAATa < 1:2999770/55‑1 (MQ=255) aCTGTCTACCGCGTTGCAATACGAGTTGCCCGTACTGGTGGTGGTGAATCTCAATAACCGCTATCTggg > 1:474356/1‑69 (MQ=255) aCTGTCTACCGCGTTGCAATACGAGTTGACCGTAC‑‑‑tggt > 1:2945090/1‑39 (MQ=255) cTGTCTACCGCGTTGCAATACGAGTTGCCCGTAC‑‑‑TGGTGGTGAATCTCAATAACCGCTATCTGGGGATg > 1:1241282/1‑69 (MQ=255) gTCTACCGCGTTGCAATACGAGTTGCCCGTAC‑‑‑TGGTGGTGAATCTCAATa < 1:3286191/50‑1 (MQ=255) gTCTACCGCGTTGCAATACGAGTTGCCCGTAC‑‑‑TGGTGGTGAATCTCAATa < 1:648576/50‑1 (MQ=255) cgTTGCAATACGAGGTGCCCGTAC‑‑‑TGGTGGTGAATCTCAATAACCGCTATCTGGGGATGGTGAAg < 1:3009448/65‑1 (MQ=255) ttGCAATACGAGTTGCCCGTAC‑‑‑TGGTGGTGAATCTCAATa < 1:1938594/40‑1 (MQ=255) aTACGAGTTGCCCGTAC‑‑‑TGGTGGTGAATCTCAATa < 1:934344/35‑1 (MQ=255) cGAGTTGCCCGTAC‑‑‑TGGTGGTGAATCTCAATAACCGCTATCTGGGGATGGTGAAGc < 1:3133066/56‑1 (MQ=255) cGAGTTGCCCGTAC‑‑‑TGGTGGTGAATCTCAATAACCGCTATCTGGGGATGGTGAAGc > 1:879852/1‑56 (MQ=255) cGAGTTGCCCGTAC‑‑‑TGGTGGTGAATCTCAATAACCGCTATCTGGGGATGGTGAAGc < 1:1812444/56‑1 (MQ=255) cGAGTTGCCCGTAC‑‑‑TGGTGGTGAATCTCAATAACCGCTATCTGGGGATGGTGAAGc < 1:2569480/56‑1 (MQ=255) cGAGTTGCCCGTAC‑‑‑TGGTGGTGAATCTCAATAACCGCTATCTGGGGATGGTGAAGc < 1:2778286/56‑1 (MQ=255) cGAGTTGCCCGTAC‑‑‑TGGTGGTGAATCTCAATAACCGCTATCTGGGGATGGTGAAGc > 1:431977/1‑56 (MQ=255) cGAGTTGCCCGTAC‑‑‑TGGTGGTGAATCTCAATAACCGCTATCTGGGGATGGTGAAGc > 1:3045450/1‑56 (MQ=255) cGAGTTGCCCGTAC‑‑‑TGGTGGTGAATCTCAATAACCGCTATCTGGGGATGGTGAAGCAGTGGCAGGACAt > 1:621685/1‑69 (MQ=255) cGAGTTGCCCGTAC‑‑‑TGGTGGTGAATCTCAATAACCGCTATCTGGGGATGGTGAAGCAGTGGCAGGACAt > 1:2902392/1‑69 (MQ=255) | CTGGCGACGGCAGTATTCAGATGAACATCCAGGAACTGTCTACCGCGTTGCAATACGAGTTGCCCGTAC‑‑‑TGGTGGTGAATCTCAATAACCGCTATCTGGGGATGGTGAAGCAGTGGCAGGACAT > minE/71706‑71829 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |