| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 686,819 | T→C | 30.1% | F19F (TTT→TTC) | yccX → | predicted acylphosphatase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 686,819 | 0 | T | C | 30.1% | 66.1 / 33.4 | 73 | F19F (TTT→TTC) | yccX | predicted acylphosphatase |
| Reads supporting (aligned to +/- strand): ref base T (27/24); new base C (10/12); total (37/36) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.16e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.54e-01 | |||||||||||
GAGAGCAGCAATGTCGAAAGTCTGCATAATTGCCTGGGTTTACGGGCGAGTTCAGGGCGTAGGATTTCGCTACACCACACAGTACGAAGCGAAAAGACTGGGTTTAACCGGGTACGCCAAAAATCTTGACGAT > minE/686753‑686885 | gagaGCAGCAATGTCGAAAGTCTGCATAATTGCCTGGGTTTACGGGCGAGTTCAGGGCGTAGGATTTc > 1:3157271/1‑68 (MQ=255)gagaGCAGCAATGTCGAAAGTCTGCATAATTGCCTGGGTTTACGGGCGAGTTCAGGGCGTAGGATTTc > 1:1101973/1‑68 (MQ=255)gagaGCAGCAATGTCGAAAGTCTGCATAATTGCCTGGGTTTACGGGCGAGTTCAGGGCGTAGGATTTc > 1:197119/1‑68 (MQ=255) agagCAGCAATGTCGAAAGTCTGCATAATTGCCTGGGTTTACGGGCGAGTTCAGGGCGTAGGATTTCGc < 1:1998994/69‑1 (MQ=255) agagCAGCAATGTCGAAAGTCTGCATAATTGCCTGGGTTTACGGGCGAGTTCAGGGCGTAGGATTTCGc < 1:3162949/69‑1 (MQ=255) agagCAGCAATGTCGAAAGTCTGCATAATTGCCTGGGTTTACGGGCGAGTTCAGGGCGTAGGATTTCGc < 1:262300/69‑1 (MQ=255) agagCAGCAATGTCGAAAGTCTGCATAATTGCCTGGGTTTACGGGCGAGTTCAGGGCGTAGGATTTCGc < 1:13288/69‑1 (MQ=255) agagCAGCAATGTCGAAAGTCTGCATAATTGCCTGGGTTTACGGGCGAGTTCAGGGCGTAGGATTCCGc < 1:2729397/69‑1 (MQ=255) agagCAGCAATGTCGAAAGTCTGCATAATTGCCTGGGTTTACGGGCGAGTTCAGGGCGTAGGATTCCGc < 1:3231512/69‑1 (MQ=255) agagCAGCAATGTCGAAAGTCTGCATAATTGCCTGGGTTTACGGGCGAGTTCAGGGCGTAGGATTCCGc < 1:2826367/69‑1 (MQ=255) agagCAGCAATGTCGAAAGTCTGCATAATTGCCTGGGTTTACGGGCGAGTTCAGGGCGTAGGATTCCGc < 1:103423/69‑1 (MQ=255) agagCAGCAATGTCGAAAGTCTGCATAATTGCCTGGGTTTACGGGCGAGTTCAGGGCGTAGGATTCCGc < 1:2836817/69‑1 (MQ=255) agagCAGCAATGTCGAAAGTCTGCATAATTGCCTGGGTTTACGGGCGAGTTCAGGGCGTAGGATTCCGc < 1:2949260/69‑1 (MQ=255) agagCAGCAATGTCGAAAGTCTGCATAATTGCCTGGGTTTACGGGCGAGTTCAGGGCGTAGGATTCCGc < 1:1967421/69‑1 (MQ=255) agagCAGCAATGTCGAAAGTCTGCATAATTGCCTGGGTTTACGGGCGAGTTCAGGGCGTAGGATTCCGc < 1:3197406/69‑1 (MQ=255) agagCAGCAATGTCGAAAGTCTGCATAATTGCCTGGGTTTACGGGCGAGTTCAGGGCGTAGGATTCCGc < 1:423777/69‑1 (MQ=255) agagCAGCAATGTCGAAAGTCTGCATAATTGCCTGGGTTTACGGGCGAGTTCAGGGCGTAGGATTCCGc < 1:563695/69‑1 (MQ=255) gagCAGCAATGTCGAAAGTCTGCATAATTGCCTGGGTTTACGGGCGAGTTCAGGGCGTAGGATTCCGc < 1:800222/68‑1 (MQ=255) agcagcAATGTCGAAAGTCTGCATAATTGCCTGGGTTTACGGGCGAGTTCAGGGCGTAGGATTTCGc < 1:1323017/67‑1 (MQ=255) agcagcAATGTCGAAAGTCTGCATAATTGCCTGGGTTTACGGGCGAGTTCAGGGCGTAGGATTTCGCTa < 1:1827082/69‑1 (MQ=255) agcagcAATGTCGAAAGTCTGCATAATTGCCTGGGTTTACGGGCGAGTTCAGGGCGTAGGATTTCGCTa < 1:1589088/69‑1 (MQ=255) agcagcAATGTCGAAAGTCTGCATAATTGCCTGGGTTTACGGGCGAGTTCAGGGCGTAGGATTTCGCTa < 1:3099412/69‑1 (MQ=255) agcagcAATGTCGAAAGTCTGCATAATTGCCTGGGTTTACGGGCGAGTTCAGGGCGTAGGATTTCGCTa < 1:2688640/69‑1 (MQ=255) agcagcAATGTCGAAAGTCTGCATAATTGCCTGGGTTTACGGGCGAGTTCAGGGCGTAGGATTTCGCTa < 1:34501/69‑1 (MQ=255) caATGTCGAAAGTCTGCATAATTGCCTGGGTTTACGGGCGAGTTCAGGGCGTAGGATTTCGCTAcacca < 1:1723173/69‑1 (MQ=255) caATGTCGAAAGTCTGCATAATTGCCTGGGTTTACGGGCGAGTTCAGGGCGTAGGATTTCGCTAcacca < 1:1138540/69‑1 (MQ=255) caATGTCGAAAGTCTGCATAATTGCCTGGGTTTACGGGCGAGTTCAGGGCGTAGGATTTCGCTAcacca < 1:3432973/69‑1 (MQ=255) caATGTCGAAAGTCTGCATAATTGCCTGGGTTTACGGGCGAGTTCAGGGCGTAGGATTTCGCTAcacca < 1:2296991/69‑1 (MQ=255) caATGTCGAAAGTCTGCATAATTGCCTGGGTTTACGGGCGAGTTCAGGGCGTAGGATTTCGCTAcacca < 1:1100890/69‑1 (MQ=255) aGTCTGCATAATTGCCTGGGTTTACGGGCGAGTTCAGGGCGTAGGATTTCGCTACACCACACAGTACGa > 1:1444469/1‑69 (MQ=255) tCTGCATAATTGGCTGGGTTTACGGGCGAGTTCAGGGCGTAGGTTTCCGc < 1:1935488/50‑1 (MQ=255) tGCATAATTGCCTGGGTTTACGGGCGAGTTCAGGGCGTAGGATTTCGCTACACCACACAGTACGAAGCg < 1:91893/69‑1 (MQ=255) aTTGCCTGGGTTTACGGGCGAGTTCAGGGCGTAGGATTTCGCTa > 1:1508676/1‑44 (MQ=255) aTTGCCTGGGTTTACGGGCGAGTTCAGGGCGTAGGATTTCGCTa > 1:1512532/1‑44 (MQ=255) aTTGCCTGGGTTTACGGGCGAGTTCAGGGCGTAGGATTTCGCTACACCACACAGTACGAAGCGAAAAg > 1:1752573/1‑68 (MQ=255) aTTGCCTGGGTTTACGGGCGAGTTCAGGGCGTAGGATTTCGCTACACCACACAGTACGAAGCGAAAAGa > 1:1323544/1‑69 (MQ=255) aTTGCCTGGGTTTACGGGCGAGTTCAGGGCGTAGGATTTCGCTACACCACACAGTACGAAGCGAAAAGa > 1:535359/1‑69 (MQ=255) aTTGCCTGGGTTTACGGGCGAGTTCAGGGCGTAGGATTTCGCTACACCACACAGTACGAAGCGAAAAGa > 1:3212931/1‑69 (MQ=255) aTTGCCTGGGTTTACGGGCGAGTTCAGGGCGTAGGATTTCGCTACACCACACAGTACGAAGCGAAAAGa > 1:2050893/1‑69 (MQ=255) aTTGCCTGGGTTTACGGGCGAGTTCAGGGCGTAGGATTTCGCTACACCACACAGTACGAAGCGAAAAGa > 1:568063/1‑69 (MQ=255) tttACGGGCGAGTTCAGGGCGTAGGATTTCGCTACACCACACAGTACGAAGCGAAAAGACTGGGTTTa < 1:2265723/68‑1 (MQ=255) ttACGGGCGAGTTCAGGGCGTAGGATTCCGCTACCCCACGCAGTACGAAGCGAAAAAACTGGGACTAAc > 1:2228387/1‑69 (MQ=255) ttACGGGCGAGTTCAGGGCGTAGGATTCCGCTACACCACGCAGTACGAAGCGAAAAAACTGGGACTAAc > 1:2926001/1‑69 (MQ=255) ttACGGGCGAGTTCAGGGCGTAGGATTCCGCTACACCACGCAGTACGAAGCGAAAAAACTGGGACTAAc > 1:109997/1‑69 (MQ=255) ttACGGGCGAGTTCAGGGCGTAGGATTCCGCTACACCACGCAGTACGAAGCGAAAAAACTGGGACTAAc > 1:2703240/1‑69 (MQ=255) ttACGGGCGAGTTCAGGGCGTAGGATTCCGCTACACCACGCAGTACGAAGCGAAAAAACTGGGACTAAc > 1:2537908/1‑69 (MQ=255) ttACGGGCGAGTTCAGGGCGTAGGATTCCGCTACACCACGCAGTACGAAGCGAAAAAACTGGGACTAAc > 1:2519649/1‑69 (MQ=255) ttACGGGCGAGTTCAGGGCGTAGGATTCCGCTACACCACGCAGTACGAAGCGAAAAAACTGGGACTAAc > 1:2474217/1‑69 (MQ=255) ttACGGGCGAGTTCAGGGCGTAGGATTCCGCTACACCACGCAGTACGAAGCGAAAAAACTGGGACTAAc > 1:38093/1‑69 (MQ=255) ttACGGGCGAGTTCAGGGCGTAGGATTCCGCTACACCACGCAGTACGAAGCGAAAAAACTGGGACTAAc > 1:598706/1‑69 (MQ=255) ttACGGGCGAGTTCAGGGCGTAGGATTCCGCTACACCACGCAGTACGAAGCGAAAAAACTGGGACTAAc > 1:1247454/1‑69 (MQ=255) tACGGGCGAGTTCAGGGCGTAGGATTTCGCTacac > 1:1292457/1‑35 (MQ=255) tACGGGCGAGTTCAGGGCGTAGGATTTCGCTACACCACACAGTACGAAGCGAAAAGACTGGGtt > 1:2648757/1‑64 (MQ=255) tACGGGCGAGTTCAGGGCGTAGGATTTCGCTACACCACACAGTACGAAGCGAAAAGACTGGGTTTAAcc > 1:2644823/1‑69 (MQ=255) tACGGGCGAGTTCAGGGCGTAGGATTTCGCTACACCACACAGTACGAAGCGAAAAGACTGGGTTTAAcc > 1:1554085/1‑69 (MQ=255) tACGGGCGAGTTCAGGGCGTAGGATTTCGCTACACAACACAGTACGAAGCGAAAAGACTGGGTTTAAcc > 1:1748363/1‑69 (MQ=255) ggCGAGTTCAGGGCGTAGGATTTCGCTACACCACACAGTACGAAGCGAAAAGACTGGGTTTAACCggg < 1:1168646/68‑1 (MQ=255) cAGGGCGTAGGATTTCGCTACACCACACAGTACGAAGCGAAAAg < 1:3117651/44‑1 (MQ=255) cAGGGCGTAGGATTTCGCTACACCACACAGTACGAAGCGAAAAg < 1:97884/44‑1 (MQ=255) cAGGGCGTAGGATTTCGCTACACCACACAGTACGAAGCGAAAAg > 1:1617405/1‑44 (MQ=255) cAGGGCGTAGGATTTCGCTACACCACACAGTACGAAGCGAAAAg > 1:574002/1‑44 (MQ=255) cAGGGCGTAGGATTTCGCTACACCACACAGTACGAAGCGAAAAg > 1:1728524/1‑44 (MQ=255) cAGGGCGTAGGATTTCGCTACACCACACAGTACGAAGCGAAAAg > 1:1900144/1‑44 (MQ=255) cAGGGCGTAGGATTTCGCTACACCACACAGTACGAAGCGAAAAg > 1:2230323/1‑44 (MQ=255) cAGGGCGTAGGATTTCGCTACACCACACAGTACGAAGCGAAAAg < 1:226957/44‑1 (MQ=255) cAGGGCGTAGGATTTCGCTACACCACACAGTACGAAGCGAAAAg > 1:2822463/1‑44 (MQ=255) cAGGGCGTAGGATTTCGCTACACCACACAGTACGAAGCGAAAAGACTGGGTTTAACCGGGTACGCCaaa > 1:2801235/1‑69 (MQ=255) cAGGGCGTAGGATTTCGCTACACCACACAGTACGAAGCGAAAAGACTGGGTTTAACCGGGTACGCCaaa > 1:918662/1‑69 (MQ=255) cAGGGCGTAGGATTTCGCTACACCACACAGTACGAAGCGAAAAGACTGGGTTTAACCGGGAACGCCaaa > 1:2866275/1‑69 (MQ=255) ggATTTCGCTACACCACACAGTACGAAGCGAAAAGACTGGGTTTAACCGGGTACGCCAAAAATCTTGAc < 1:3324400/69‑1 (MQ=255) ggATTTCGCTACACCACACAGTACGAAGCGAAAAGACTGGGTTTAACCGGGTACGCCAAAAATCTTGAc > 1:1674231/1‑69 (MQ=255) ggATTTCGCTACACCACACAGTACGAAGCGAAAAGACTGGGTTTAACCGGGTACGCCAAAAATCTTGAc < 1:3247343/69‑1 (MQ=255) ggATTTCGCTACACCACACAGTACGAAGCGAAAAGACTGGGTTTAACCGGGTACGCCAAAAATCTTGAc < 1:3242674/69‑1 (MQ=255) tttCGCTACACCACACAGTACGAAGCGAAAAGACTGGGTTTAACCGGGTACGCCAAAAATCTTGACGAt > 1:641945/1‑69 (MQ=255) tttCGCTACACCACACAGTACGAAGCGAAAAGACTGGGTTTAACCGGGTACGCCAAAAATCTTGACGAt > 1:3145046/1‑69 (MQ=255) tttCGCGACACCACACAGTACGAAGCGAAAAGACTGGGTTTAACCGGGTACGCCAAAAATCTTGACGAt > 1:436282/1‑69 (MQ=255) | GAGAGCAGCAATGTCGAAAGTCTGCATAATTGCCTGGGTTTACGGGCGAGTTCAGGGCGTAGGATTTCGCTACACCACACAGTACGAAGCGAAAAGACTGGGTTTAACCGGGTACGCCAAAAATCTTGACGAT > minE/686753‑686885 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |