| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 691,194 | G→A | 61.8% | A414A (GCG→GCA) | hyaB → | hydrogenase 1, large subunit |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 691,194 | 0 | G | A | 61.8% | 31.4 / 43.6 | 68 | A414A (GCG→GCA) | hyaB | hydrogenase 1, large subunit |
| Reads supporting (aligned to +/- strand): ref base G (10/16); new base A (27/15); total (37/31) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.75e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.33e-01 | |||||||||||
AACGCTACTCGTGGATCAAAGCGCCACGCTGGCGCGGTAACGCGATGGAAGTGGGGCCGCTGGCGCGCACGTTAATCGCTTATCACAAAGGCGATGCTGCGACCG > minE/691130‑691234 | aaCGCTACTCGTGGATCAAAGCGCCACGCTGGCGCGGTAACGCGATGGAAGTGGGGCCGCTGGCGCGCa < 1:1073374/69‑1 (MQ=255)aaCGCTACTCGTGGATCAAAGCGCCACGCTGGCGCGGTAACGCGATGGAAGTGGGGCCGCTGGCGCGCa < 1:962670/69‑1 (MQ=255)aaCGCTACTCGTGGATCAAAGCGCCACGCTGGCGCGGTAACGCGATGGAAGTGGGGCCGCTGGCGCGCa < 1:380600/69‑1 (MQ=255)aaCGCTACTCGTGGATCAAAGCGCCACGCTGGCGCGGTAACGCGATGGAAGTGGGGCCGCTGGCGCGCa < 1:3380584/69‑1 (MQ=255)aaCGCTACTCGTGGATCAAAGCGCCACGCTGGCGCGGTAACGCGATGGAAGTGGGGCCGCTGGCGCGCa < 1:1194113/69‑1 (MQ=255) cGTGGATCAAAGCGCCACGCTGGCGCGGTAACGCGATGGAAGTGGGGCCGCTGGCGCGCACGTTAATCg > 1:1253172/1‑69 (MQ=255) gATCAAAGCGCCACTCTGGCGCGGTAACGCGATGGAAGTGGGGCCGCTGGCGCGCACGTTAATCGCtt > 1:2959990/1‑68 (MQ=255) gATCAAAGCGCCACGCTGGCGCGGTAACGCGATGGAAGTGGGGCCGCTGGCGCGCACGTTAATCGCtt > 1:1911220/1‑68 (MQ=255) gATCAAAGCGCCACGCTGGCGCGGTAACGCGATGGAAGTGGGGCCGCTGGCGCGCACGTTAATCGCtt > 1:2678882/1‑68 (MQ=255) gATCAAAGCGCCACGCTGGCGCGGTAACGCGATGGAAGTGGGGCCGCTGGCGCGCACGTTAATCGCtt > 1:1380482/1‑68 (MQ=255) gATCAAAGCGCCACGCTGGCGCGGTAACGCGATGGAAGTGGGGCCGCTGGCGCGCACGTTAATCGCtt > 1:3245645/1‑68 (MQ=255) gATCAAAGCGCCACGCTGGCGCGGTAACGCGATGGAAGTGGGGCCGCTGGCGCGCACGTTAATCGCtt > 1:23869/1‑68 (MQ=255) gATCAAAGCGCCACGCTGGCGCGGTAACGCGATGGAAGTGGGGCCGCTGGCGCGCACGTTAATCGCtt > 1:705851/1‑68 (MQ=255) gATCAAAGCGCCACGCTGGCGCGGTAACGCGATGGAAGTGGGGCCGCTGGCGCGCACGTTAATCGCtt > 1:2500614/1‑68 (MQ=255) gATCAAAGCGCCACGCTGGCGCGGTAACGCGATGGAAGTGGGGCCGCTGGCGCGCACGTTAATCGCtt > 1:1529586/1‑68 (MQ=255) aaGCGCCACGCTGGCGCGGTAACGCGATGGAAGTGGGGCCGCTGGCACGCACGTTAATc > 1:2803746/1‑59 (MQ=255) aaGCGCCACGCTGGCGCGGTAACGCGATGGAAGTGGGGCCGCTGGCACGCACGTTAATCGCTTATca > 1:2520197/1‑67 (MQ=255) aaGCGCCACGCTGGCGCGGTAACGCGATGGAAGTGGGGCCGCTGGCACGCACGTTAATCGCTTATca > 1:2462620/1‑67 (MQ=255) aaGCGCCACGCTGGCGCGGTAACGCGATGGAAGTGGGGCCGCTGGCACGCACGTTAATCGCTTATca > 1:3216975/1‑67 (MQ=255) aaGCGCCACGCTGGCGCGGTAACGCGATGGAAGTGGGGCCGCTGGCACGCACGTTAATCGCTTATca > 1:3038861/1‑67 (MQ=255) aaGCGCCACGCTGGCGCGGTAACGCGATGGAAGTGGGGCCGCTGGCACGCACGTTAATCGCTTATca > 1:2293259/1‑67 (MQ=255) aaGCGCCACGCTGGCGCGGTAACGCGATGGAAGTGGGGCCGCTGGCACGCACGTTAATCGCTTATca > 1:2554443/1‑67 (MQ=255) aaGCGCCACGCTGGCGCGGTAACGCGATGGAAGTGGGGCCGCTGGCACGCACGTTAATCGCTTATca > 1:2048544/1‑67 (MQ=255) aaGCGCCACGCTGGCGCGGTAACGCGATGGAAGTGGGGCCGCTGGCACGCACGTTAATCGCTTATca > 1:2174973/1‑67 (MQ=255) aaGCGCCACGCTGGCGCGGTAACGCGATGGAAGTGGGGCCGCTGGCACGCACGTTAATCGCTTATca > 1:2154910/1‑67 (MQ=255) aaGCGCCACGCTGGCGCGGTAACGCGATGGAAGTGGGGCCGCTGGCACGCACGTTAATCGCTTATca > 1:2133456/1‑67 (MQ=255) aaGCGCCACGCTGGCGCGGTAACGCGATGGAAGTGGGGCCGCTGGCACGCACGTTAATCGCTTATca > 1:3259025/1‑67 (MQ=255) aaGCGCCACGCTGGCGCGGTAACGCGATGGAAGTGGGGCCGCTGGCACGCACGTTAATCGCTTATca > 1:3314666/1‑67 (MQ=255) aaGCGCCACGCTGGCGCGGTAACGCGATGGAAGTGGGGCCGCTGGCACGCACGTTAATCGCTTATca > 1:1752561/1‑67 (MQ=255) aaGCGCCACGCTGGCGCGGTAACGCGATGGAAGTGGGGCCGCTGGCACGCACGTTAATCGCTTATca > 1:1246531/1‑67 (MQ=255) aaGCGCCACGCTGGCGCGGTAACGCGATGGAAGTGGGGCCGCTGGCACGCACGTTAATCGCTTATca > 1:80295/1‑67 (MQ=255) aaGCGCCACGCTGGCGCGGTAACGCGATGGAAGTGGGGCCGCTGGCACGCACGTTAATCGCTTATca > 1:14010/1‑67 (MQ=255) aaGCGCCACGCTGGCGCGGTAACGCGATGGAAGTGGGGCCGCTGGCACGCACGTTAATCGCTTATca > 1:1432075/1‑67 (MQ=255) aaGCGCCACGCTGGCGCGGTAACGCGATGGAAGTGGGGCCGCTGGCACGCACGTTAATCGCTTATca > 1:1456936/1‑67 (MQ=255) aaGCGCCACGCTGGCGCGGTAACGCGATGGAAGTGGGGCCGCTGGCACGCACGTTAATCGCTTATca > 1:766517/1‑67 (MQ=255) aaGCGCCACGCTGGCGCGGTAACGCGATGGAAGTGGGGCCGCTGGCACGCACGTTAATCGCTTATca > 1:1492736/1‑67 (MQ=255) aaGCGCCACGCTGGCGCGGTAACGCGATGGAAGTGGGGCCGCTGGCACGCACGTTAATCGCTTATca > 1:1680826/1‑67 (MQ=255) aaGCGCCACGCTGGCGCGGTAACGCGATGGAAGTGGGGCCGCTGGCACGCACGTTAATCGCTTATca > 1:596536/1‑67 (MQ=255) aaGCGCCACGCTGGCGCGGTAACGCGATGGAAGTGGGGCCGCTGGCACGCACGTTAATCGCTTATca > 1:566643/1‑67 (MQ=255) aaGCGCCACGCTGGCGCGGTAACGCGATGGAAGTGGGGCCGCTGGCACGCACGTTAATCGCTTATca > 1:2003871/1‑67 (MQ=255) aaGCGCCACGCTGGCGCGGTAACGCGATGGAAGTGGGGCCGCTGGCACGCACGTTAATCGCTTATca > 1:1118254/1‑67 (MQ=255) aaGCGCAACGCTGGCGCGGTAACGCGATGGAAGTGGGGCCGCTGGCACGCACGTTAATCGCTTATca > 1:437319/1‑67 (MQ=255) cgcCACGCTGGCGCGGTAACGCGATGGAAGTGGGGCC‑CTGGCACGCACGTTAATCGCTTATCACAAAgg < 1:1991758/69‑1 (MQ=255) gcCACGCTGGCGCGGTAACGCGATGGAAGTGGGGCCGCTGGCACGCACGTTAATCGCTTATCACAAAgg < 1:3319972/69‑1 (MQ=255) gcCACGCTGGCGCGGTAACGCGATGGAAGTGGGGCCGCTGGCACGCACGTTAATCGCTTATCACAAAgg < 1:422045/69‑1 (MQ=255) gcCACGCTGGCGCGGTAACGCGATGGAAGTGGGGCCGCTGGCACGCACGTTAATCGCTTATCACAAAgg < 1:2994613/69‑1 (MQ=255) gcCACGCTGGCGCGGTAACGCGATGGAAGTGGGGCCGCTGGCACGCACGTTAATCGCTTATCACAAAgg < 1:2392063/69‑1 (MQ=255) gcCACGCTGGCGCGGTAACGCGATGGAAGTGGGGCCGCTGGCACGCACGTTAATCGCTTATCACAAAgg < 1:2543818/69‑1 (MQ=255) gcCACGCTGGCGCGGTAACGCGATGGAAGTGGGGCCGCTGGCACGCACGTTAATCGCTTATCACAAAgg < 1:2197968/69‑1 (MQ=255) gcCACGCTGGCGCGGTAACGCGATGGAAGTGGGGCCGCTGGCACGCACGTTAATCGCTTATCACAAAgg < 1:2079245/69‑1 (MQ=255) gcCACGCTGGCGCGGTAACGCGATGGAAGTGGGGCCGCTGGCACGCACGTTAATCGCTTATCACAAAgg < 1:1329643/69‑1 (MQ=255) gcCACGCTGGCGCGGTAACGCGATGGAAGTGGGGCCGCTGGCACGCACGTTAATCGCTTATCACAAAgg < 1:147897/69‑1 (MQ=255) gcCACGCTGGCGCGGTAACGCGATGGAAGTGGGGCCGCTGGCACGCACGTTAATCGCTTATCACAAAgg < 1:2844974/69‑1 (MQ=255) gcCACGCTGGCGCGGTAACGCGATGGAAGTGGGGCCGCTGGCACGCACGTTAATCGCTTATCACAAAgg < 1:1897438/69‑1 (MQ=255) gcCACGCTGGCGCGGTAACGCGATGGAAGTGGGGCCGCTGGCACGCACGTTAATCGCTTATCACAAAgg < 1:1844263/69‑1 (MQ=255) ccACGCTGGCGCGGTAACGCGATGGAAGTGGGGCCGCTGGCACGCACGTTAATCGCTTATCACAAAgg < 1:757717/68‑1 (MQ=255) cgGTAACGCGATGGAAGTGGGGCCGCTGGCGCGCACGTTAATCGCTTATCACAAAGGCGATGCTGCGAc < 1:2521758/69‑1 (MQ=255) cgGTAACGCGATGGAAGTGGGGCCGCTGGCGCGCACGTTAATCGCTTATCACAAAGGCGATGCTGCGAc < 1:572950/69‑1 (MQ=255) gTAACGCGATGGATGTGGGGCCGCTGGCGCGCACGTTAATCGCTTATCACAAAGGCGATGCTGCGACCg < 1:2602077/69‑1 (MQ=255) gTAACGCGATGGAAGTGGGGCCGCTGGCACGCACGTTAATCGCTTATCACAAAgg < 1:205204/55‑1 (MQ=255) aaCGCGATGGAAGTGGGGCCGCTGGCGCGCACGTTAATCGCTTATCACAAAGGCGAt < 1:1091972/57‑1 (MQ=255) aaCGCGATGGAAGTGGGGCCGCTGGCGCGCACGTTAATCGCTTATCACAAAGGCGAt < 1:1051755/57‑1 (MQ=255) aaCGCGATGGAAGTGGGGCCGCTGGCGCGCACGTTAATCGCTTATCACAAAGGCGAt < 1:149107/57‑1 (MQ=255) aaCGCGATGGAAGTGGGGCCGCTGGCGCGCACGTTAATCGCTTATCACAAAGGCGAt < 1:1820291/57‑1 (MQ=255) aaCGCGATGGAAGTGGGGCCGCTGGCGCGCACGTTAATCGCTTATCACAAAGGCGAt < 1:1865591/57‑1 (MQ=255) aaCGCGATGGAAGTGGGGCCGCTGGCGCGCACGTTAATCGCTTATCACAAAGGCGAt < 1:2065336/57‑1 (MQ=255) aaCGCGATGGAAGTGGGGCCGCTGGCGCGCACGTTAATCGCTTATCACAAAGGCGAt < 1:2075979/57‑1 (MQ=255) tGGCGCGCACGTTAATCGCTTATCACAAAGGCGATGCTGCGAc < 1:2623436/43‑1 (MQ=255) | AACGCTACTCGTGGATCAAAGCGCCACGCTGGCGCGGTAACGCGATGGAAGTGGGGCCGCTGGCGCGCACGTTAATCGCTTATCACAAAGGCGATGCTGCGACCG > minE/691130‑691234 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |