| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 693,202 | G→A | 27.8% | E50E (GAG→GAA) | hyaE → | protein involved in processing of HyaA and HyaB proteins |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 693,202 | 0 | G | A | 27.8% | 72.9 / 25.5 | 68 | E50E (GAG→GAA) | hyaE | protein involved in processing of HyaA and HyaB proteins |
| Reads supporting (aligned to +/- strand): ref base G (38/11); new base A (13/6); total (51/17) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 5.35e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
GACGACTGGCTTACGCAAGCGCCAGACGGCGTGGTGTTATTAAGCAGTGACCCGAAACGCACGCCAGAGGTCAGCGATAATCCGGTAATGATTGGCGAATTACTGCGCGAGTTTCCCGACTATA > minE/693134‑693257 | gacgacTGGCTTACGCAAGCGCCAGACGGCGTGGTGTTATTAAGCAGTGACCCGAAACGCACGCCagag > 1:1509976/1‑69 (MQ=255) gacTGGCTTACGCAAGCGCCAGACGGCGTGGTGTTATTAAGCAGCGATCCGAAACGCACGCCAGAAGt < 1:912131/68‑1 (MQ=255) gacTGGCTTACGCAAGCGCCAGACGGCGTGGTGTTATTAAGCAGCGATCCGAAACGCACGCCAGAAGt < 1:770815/68‑1 (MQ=255) gacTGGCTTACGCAAGCGCCAGACGGCGTGGTGTTATTAAGCAGCGATCCGAAACGCACGCCAGAAGt < 1:1242810/68‑1 (MQ=255) gacTGGCTTACGCAAGCGCCAGACGGCGTGGTGTTATTAAGCAGCGATCCGAAACGCACGCCAGAAGt < 1:2552563/68‑1 (MQ=255) gacTGGCTTACGCAAGCGCCAGACGGCGTGGTGTTATTAAGCAGCGATCCGAAACGCACGCCAGAAGt < 1:2597378/68‑1 (MQ=255) gacGGGCTTACGCAAGCGCCAGACGGCGTGGTGTTATTAAGCAGCGATCCGAAACGCACGCCAGAAGt < 1:2155542/68‑1 (MQ=255) tGGCTTACGCAAGCGCCAGACGGCGTGGTGTTATTAAGCAGTGACCCGAAACGCACGCCAGAGGTCAGc > 1:745150/1‑69 (MQ=255) tGGCTTACGCAAGCGCCAGACGGCGTGGTGTTATTAAGCAGTGACCCGAAACGCACGCCAGAGGTCAGc > 1:1383839/1‑69 (MQ=255) ggCTTACGCAAGCGCCAGACGGCGTGGTGTTATTAAGCAGTGACCCGAAACGCACGCCAGAGGTCAgg > 1:2139725/1‑67 (MQ=255) ggCTTACGCAAGCGCCAGACGGCGTGGTGTTATTAAGCAGTGACCCGAAACGCACGCCAGAGGTCAGc > 1:1872029/1‑68 (MQ=255) ggCTTACGCAAGCGCCAGACGGCGTGGTGTTATTAAGCAGTGACCCGAAACGCACGCCAGAGGTCAGc > 1:2836956/1‑68 (MQ=255) ttACGCAAGCGCCAGACGGCGTGGTGTTATTAAGCAGTGACCCGAAACGCACGCCAGAGGTCAGCGAt < 1:2939475/68‑1 (MQ=255) ttACGCAAGCGCCAGACGGCGTGGTGTTATTAAGCAGTGACCCGAAACGCACGCCAGAGGTCAGCGAt < 1:336062/68‑1 (MQ=255) ttACGCAAGCGCCAGACGGCGTGGTGTTATTAAGCAGTGACCCGAAACGCACGCCAGAGGTCAGCGAt < 1:354177/68‑1 (MQ=255) ttACGCAAGCGCCAGACGGCGTGGTGTTATTAAGCAGTGACCCGAAACGCACGCCAGAGGTCAGCGAt < 1:820389/68‑1 (MQ=255) ttACGCAAGCGCCAGACGGCGTGGTGTTATTAAGCAGTGACCCGAAACGCACGCCAGAGGTCAGCGAt < 1:1152632/68‑1 (MQ=255) ttACGCAAGCGCCAGACGGCGTGGTGTTATTAAGCAGTGACCCGAAACGCACGCCAGAGGTCAGCGAt < 1:1637519/68‑1 (MQ=255) ttACGCAAGCGCCAGACGGCGTGGTGTTATTAAGCAGTGACCCGAAACGCACGCCAGAGGTCAGCGAt < 1:1702856/68‑1 (MQ=255) ttACGCAAGCGCCAGACGGCGTGGTGTTATTAAGCAGTGACCCGAAACGCACGCCAGAGGTCAGCGAt < 1:1506198/68‑1 (MQ=255) aGCGCCAGACGGCGTGGTGTTATTAAGCAGTGACCCGAAACGCACGCCAGAGGTCAGCGATAATCCGGt > 1:52776/1‑69 (MQ=255) aGCGCCAGACGGCGTGGTGTTATTAAGCAGTGACCCGAAACGCACGCCAGAGGTCAGCGATAATCCGGt > 1:1162462/1‑69 (MQ=255) aGCGCCAGACGGCGTGGTGTTATTAAGCAGTGACCCGAAACGCACGCCAGAGGTCAGCGATAATCCGGt > 1:327556/1‑69 (MQ=255) aGCGCCAGACGGCGTGGTGTTATTAAGCAGTGACCCGAAACGCACGCCAGAGGTCAGCGATAATCCGGt > 1:1816993/1‑69 (MQ=255) aGCGCCAGACGGCGTGGTGTTATTAAGCAGTGACCCGAAACGCACGCCAGAGGTCAGCGATAATCCGGt > 1:2118277/1‑69 (MQ=255) aGCGCCAGACGGCGTGGTGTTATTAAGCAGTGACCCGAAACGCACGCCAGAGGTCAGCGATAATCCGGt > 1:2768517/1‑69 (MQ=255) tgtTATTAAGCAGTGACCCGAAACGCACGCCAGAGGTCAGCGATAATCCGGTAATGATTGGCGAATTAc > 1:2642634/1‑69 (MQ=255) tgtTATTAAGCAGTGACCCGAAACGCACGCCAGAGGTCAGCGATAATCCGGTAATGATTGGCGAATTAc > 1:274360/1‑69 (MQ=255) tgtTATTAAGCAGTGACCCGAAACGCACGCCAGAGGTCAGCGATAATCCGGTAATGATTGGCGAATTAc > 1:585327/1‑69 (MQ=255) tgtTATTAAGCAGTGACCCGAAACGCACGCCAGAGGTCAGCGATAATCCGGTAATGATTGGCGAATTAc > 1:645129/1‑69 (MQ=255) tgtTATTAAGCAGTGACCCGAAACGCACGCCAGAGGTCAGCGATAATCCGGTAATGATTGGCGAATTAc > 1:1832995/1‑69 (MQ=255) attaAGCAGTGACGCGAAACGCACGCCAGAGGTCAGCGATAATCCGGTAATGATTGGCGAATTACTgc > 1:1193961/1‑68 (MQ=255) attaAGCAGTGACCCGAAACGCACGCCAGAGGTCAGCGATAATCCGGTAATGATTGGCGAATTACTgc > 1:378489/1‑68 (MQ=255) attaAGCAGTGACCCGAAACGCACGCCAGAGGTCAGCGATAATCCGGTAATGATTGGCGAATTACTgc > 1:1461796/1‑68 (MQ=255) attaAGCAGTGACCCGAAACGCACGCCAGAGGTCAGCGATAATCCGGTAATGATTGGCGAATTACTgc > 1:3191938/1‑68 (MQ=255) attaAGCAGTGACCCGAAACGCACGCCAGAGGTCAGCGATAATCCGGTAATGATTGGCGAATTACTgc > 1:1826243/1‑68 (MQ=255) attaAGCAGTGACCCGAAACGCACGCCAGAGGTCAGCGATAATCCGGTAATGATTGGCGAATTACTgc > 1:1994538/1‑68 (MQ=255) attaAGCAGTGACCCGAAACGCACGCCAGAGGTCAGCGATAATCCGGTAATGATTGGCGAATTACTg > 1:321258/1‑67 (MQ=255) attaAGCAGTGACCCGAAACGCACGCCAGAGGTCAGCGATAATCCGGTAATGATTGGCGAATTACTg > 1:3224281/1‑67 (MQ=255) attaAGCAGTGACCCGAAACGCACGCCAGAGGTCAGCGATAATCCAGTAATGATTGGCGAATTACTgc > 1:2163175/1‑68 (MQ=255) aaGCAGTGACCCGAAACGCACGCCAGAGGTCAGCGATAATCCGGTAATGATTGGCGAATTACTGcgcg > 1:468371/1‑68 (MQ=255) aaGCAGTGACCCGAAACGCACGCCAGAGGTCAGCGATAATCCGGTAATGATTGGCGAATTACTGCGCGa > 1:2549116/1‑69 (MQ=255) aaGCAGTGACCCGAAACGCACGCCAGAGGTCAGCGATAATCCGGTAATGATTGGCGAATTACTGCGCGa > 1:364589/1‑69 (MQ=255) gTGACCCGAAACGCACGCCAGAGGTCAGCGATAATCCGGTAATGATTGGCGAATTACTGCGCGAGTTTc > 1:511857/1‑69 (MQ=255) gTGACCCGAAACGCACGCCAGAGGTCAGCGATAATCCGGTAATGATTGGCGAATTACTGCGCGAGTTTc > 1:597995/1‑69 (MQ=255) aCCCGAAACGCACGCCAGAGGTCAGCGATAATCCGGTAATGATTGGCGAATTACTGCGCGAGTTTccc > 1:694577/1‑68 (MQ=255) aCCCGAAACGCACGCCAGAGGTCAGCGATAATCCGGTAATGATTGGCGAATTACTGCGCGAGTTTCCCg > 1:124616/1‑69 (MQ=255) aCCCGAAACGCACGCCAGAGGTCAGCGATAATCCGGTAATGATTGGCGAATTACTGCGCGAGTTTCCCg > 1:810849/1‑69 (MQ=255) aCCCGAAACGCACGCCAGAGGTCAGCGATAATCCGGTAATGATTGGCGAATTACTGCGCGAGTTTCCCg > 1:3113472/1‑69 (MQ=255) aCCCGAAACGCACGCCAGAGGTCAGCGATAATCCGGTAATGATTGGCGAATTACTGCGCGAGTTTCCCg > 1:1888816/1‑69 (MQ=255) aCCCGAAACGCACGCCAGAGGTCAGCGATAATCCGGTAATGATTGGCGAATTACTGCGCGAGTTTCCCg > 1:2073245/1‑69 (MQ=255) aCCCGAAACGCACGCCAGAGGTCAGCGATAATCCGGTAATGATTGGCGAATTACTGCGCGAGTTTCCCg > 1:2795330/1‑69 (MQ=255) aCCCGAAACGCACGCCAGAGGTCAGCGATAATCCGGTAATGATTGGCGAATTACTGCGCGAGTTTCCCg > 1:1530409/1‑69 (MQ=255) ccGAAACGCACGCCAGAAGTCAGCGATAACCCGGTAATGATTGGCGAATTACTGCGCGAGTTTCCCg > 1:1117548/1‑67 (MQ=255) ccGAAACGCACGCCAGAAGTCAGCGATAACCCGGTAATGATTGGCGAATTACTGCGCGAGTTTCCCGAc > 1:2463730/1‑69 (MQ=255) ccGAAACGCACGCCAGAAGTCAGCGATAACCCGGTAATGATTGGCGAATTACTGCGCGAGTTTCCCGAc > 1:986961/1‑69 (MQ=255) ccGAAACGCACGCCAGAAGTCAGCGATAACCCGGTAATGATTGGCGAATTACTGCGCGAGTTTCCCGAc > 1:1680821/1‑69 (MQ=255) ccGAAACGCACGCCAGAAGTCAGCGATAACCCGGTAATGATTGGCGAATTACTGCGCGAGTTTCCCGAc > 1:2246998/1‑69 (MQ=255) ccGAAACGCACGCCAGAAGTCAGCGATAACCCGGTAATGATTGGCGAATTACTGCGCGAGTTTCCCGAc > 1:2318411/1‑69 (MQ=255) ccGAAACGCACGCCAGAAGTCAGCGATAACCCGGTAATGATTGGCGAATTACTGCGCGAGTTTCCCGAc > 1:3190323/1‑69 (MQ=255) ccGAAACGCACGCCAGAAGTCAGCGATAACCCGGTAATGATTGGCGAATTACTGCGCGAGTTTCCCGAc > 1:2690789/1‑69 (MQ=255) ccGAAACGCACGCCAGAAGTCAGCGATAACCCGGTAATGATTGGCGAATTACTGCGCGAGTTTCCCGAc > 1:1505381/1‑69 (MQ=255) ccGAAACGCACGCCAGAAGTCAGCGATAACCCGGTAATGATTGGCGAATTACTGCGCGAGTTTCCCGAc > 1:364041/1‑69 (MQ=255) ccGAAACGCACGCCAGAAGTCAGCGATAACCCGGTAATGATTGGCGAATTACTGCGCGAGTTTCCCGAc > 1:3148236/1‑69 (MQ=255) ccGAAACGCACGCCAGAAGTCAGCGATAACCCGGTAATGATTGGCGAATTACTGCGCGAGTTTCCCGAc > 1:1500049/1‑69 (MQ=255) ccGAAACGCACGCCAGAAGTCAGCGATAACCCGGTAATGATTGGCGAATTACTGCGCGAGTTTCCCGAc > 1:3348315/1‑69 (MQ=255) aaCGCACGCCAGAGGTCAGCGATAATCCGGTAATGATTGGCGAATTACTGCGCGAGTTTCCCGACtata < 1:3068033/69‑1 (MQ=255) aaCGCACGCCAGAGGTCAGCGATAATCCGGTAATGATTGGCGAATTACTGCGCGAGTTTCCCGACtata < 1:1927516/69‑1 (MQ=255) aaCGCACGCCAGAGGTCAGCGATAATCCGGTAATGATTGGCGAATTACTGCGCGAGTTTCCCGACtata < 1:1788751/69‑1 (MQ=255) | GACGACTGGCTTACGCAAGCGCCAGACGGCGTGGTGTTATTAAGCAGTGACCCGAAACGCACGCCAGAGGTCAGCGATAATCCGGTAATGATTGGCGAATTACTGCGCGAGTTTCCCGACTATA > minE/693134‑693257 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |