| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 694,044 | T→C | 32.5% | D199D (GAT→GAC) | hyaF → | protein involved in nickel incorporation into hydrogenase‑1 proteins |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 694,044 | 0 | T | C | 32.5% | 71.6 / 47.0 | 89 | D199D (GAT→GAC) | hyaF | protein involved in nickel incorporation into hydrogenase‑1 proteins |
| Reads supporting (aligned to +/- strand): ref base T (38/22); new base C (15/14); total (53/36) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 3.59e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
GTACGTAACCCCGACGCGCAGCCGCACAGCATTAATCTGACGCAATTACCCATCAGCGAGGCTGATCGGCTTTTTCTCTCACGTCTCTGTGGGCCGGGAAATATTCAGATTCGTACCATTGGC > minE/693979‑694101 | gTACGTAACCCCGACGCGCAGCCGCACAGCATTAATCTGACTCAATTACCCATCAGCGAAGCTGACCgg < 1:1910631/69‑1 (MQ=255)gTACGTAACCCCGACGCGCAGCCGCACAGCATTAATCTGACTCAATTACCCATCAGCGAAGCTGACCgg < 1:2362975/69‑1 (MQ=255)gTACGTAACCCCGACGCGCAGCCGCACAGCATTAATCTGACTCAATTACCCATCAGCGAAGCTGACCgg < 1:1785991/69‑1 (MQ=255)gTACGTAACCCCGACGCGCAGCCGCACAGCATTAATCTGACTCAATTACCCATCAGCGAAGCTGACCgg < 1:1860381/69‑1 (MQ=255)gTACGTAACCCCGACGCGCAGCCGCACAGCATTAATCTGACTCAATTACCCATCAGCGAAGCTGACCgg < 1:250439/69‑1 (MQ=255)gTACGTAACCCCGACGCGCAGCCGCACAGCATTAATCTGACTCAATTACCCATCAGCGAAGCTGACCgg < 1:1861363/69‑1 (MQ=255)gTACGTAACCCCGACGCGCAGCCGCACAGCATTAATCTGACTCAATTACCCATCAGCGAAGCTGACCgg < 1:3324717/69‑1 (MQ=255)gTACGTAACCCCGACGCGCAGCCGCACAGCATTAATCTGACTCAATTACCCATCAGCGAAGCTGACCgg < 1:1334089/69‑1 (MQ=255)gTACGTAACCCCGACGCGCAGCCGCACAGCATTAATCTGACTCAATTACCCATCAGCGAAGCTGACCgg < 1:586782/69‑1 (MQ=255)gTACGTAACCCCGACGCGCAGCCGCACAGCATTAATCTGACTCAATTACCCATCAGCGAAGCTGACCgg < 1:719847/69‑1 (MQ=255)gTACGTAACCCCGACGCGCAGCCGCACAGCATTAATCTGACTCAATTACCCATCAGCGAAGCTGACCgg < 1:1211086/69‑1 (MQ=255)gTACGTAACCCCGACGCGCAGCCGCACAGCATTAATCTGACTCAATTACCCATCAGCGAAGCTGACCgg < 1:868518/69‑1 (MQ=255)gTACGTAACCCCGACGCGCAGCCGCACAGCATTAATCTGACTCAATTACCCATCAGCGAAGCTGACCgg < 1:1139349/69‑1 (MQ=255) tACGTAACCCCGACGCGCAGCCGCACAGCATTAATCTGACTCAATTACCCATCAGCGAAGCTGACCgg < 1:2811832/68‑1 (MQ=255) aaCCCCGACGCGCAGCCGCACAGCATTAATCTGACGCAATTACCCATCAGCGAGGCTGATCGGCttttt > 1:1330084/1‑69 (MQ=255) aaCCCCGACGCGCAGCCGCACAGCATTAATCTGACGCAATTACCCATCAGCGAGGCTGATCGGCttttt > 1:1970939/1‑69 (MQ=255) aaCCCCGACGCGCAGCCGCACAGCATTAATCTGACGCAATTACCCATCAGCGAGGCTGATCGGCttttt > 1:3364214/1‑69 (MQ=255) aaCCCCGACGCGCAGCCGCACAGCATTAATCTGACGCAATTACCCATCAGCGAGGCTGATCGGCttttt > 1:3314201/1‑69 (MQ=255) aaCCCCGACGCGCAGCCGCACAGCATTAATCTGACGCAATTACCCATCAGCGAGGCTGATCGGCttttt > 1:3092119/1‑69 (MQ=255) aaCCCCGACGCGCAGCCGCACAGCATTAATCTGACGCAATTACCCATCAGCGAGGCTGATCGGCttttt > 1:2993736/1‑69 (MQ=255) aaCCCCGACGCGCAGCCGCACAGCATTAATCTGACGCAATTACCCATCAGCGAGGCTGATCGGCttttt > 1:2877106/1‑69 (MQ=255) ccGACGCGCAGCCGCACAGCATTAATCTGACGCAATTACCCATCAGCGAGGCTGATCGGCTTTTtctct > 1:2151829/1‑69 (MQ=255) ccGACGCGCAGCCGCACAGCATTAATCTGACGCAATTACCCATCAGCGAGGCTGATCGGCTTTTtctct > 1:2155610/1‑69 (MQ=255) ccGACGCGCAGCCGCACAGCATTAATCTGACGCAATTACCCATCAGCGAGGCTGATCGGCTTTTtctct > 1:3004169/1‑69 (MQ=255) ccGACGCGCAGCCGCACAGCATTAATCTGACGCAATTACCCATCAGCGAGGCTGATCGGCTTTTtctct > 1:2328235/1‑69 (MQ=255) ccGACGCGCAGCCGCACAGCATTAATCTGACGCAATTACCCATCAGCGAGGCTGATCGGCTTTTtctct > 1:2376249/1‑69 (MQ=255) ccGACGCGCAGCCGCACAGCATTAATCTGACGCAATTACCCATCAGCGAGGCTGATCGGCTTTTtctct > 1:2826724/1‑69 (MQ=255) ccGACGCGCAGCCGCACAGCATTAATCTGACGCAATTACCCATCAGCGAGGCTGATCGGCTTTTtctct > 1:1088841/1‑69 (MQ=255) ccGACGCGCAGCCGCACAGCATTAATCTGACGCAATTACCCATCAGCGAGGCTGATCGGCTTTTtctct > 1:3274832/1‑69 (MQ=255) ccGACGCGCAGCCGCACAGCATTAATCTGACGCAATTACCCATCAGCGAGGCTGATCGGCTTTTtctct > 1:3427225/1‑69 (MQ=255) ccGACGCGCAGCCGCACAGCATTAATCTGACGCAATTACCCATCAGCGAGGCTGATCGGCTTTTtctct > 1:890571/1‑69 (MQ=255) ccGACGCGCAGCCGCACAGCATTAATCTGACGCAATTACCCATCAGCGAGGCTGATCGGCTTTTtctct > 1:1902930/1‑69 (MQ=255) ccGACGCGCAGCCGCACAGCATTAATCTGACGCAATTACCCATCAGCGAGGCTGATCGGCTTTTtctct > 1:1388050/1‑69 (MQ=255) ccGACGCGCAGCCGCACAGCATTAATCTGACGCAATTACCCATCAGCGAGGCTGATCGGCTTTTtctct > 1:130072/1‑69 (MQ=255) gACGCGCAGCCGCACATCATTAATCTGACGCAATTACCCATCAGCGAGGCTGATCGGCTTTTTCTCTCa < 1:360955/69‑1 (MQ=255) gACGCGCAGCCGCACAGCATTAATCTGACGCAATTACCCATCAGCGAGGCTGATCGGCTTTTTCTCTCa < 1:2674086/69‑1 (MQ=255) gACGCGCAGCCGCACAGCATTAATCTGACGCAATTACCCATCAGCGAGGCTGATCGGCTTTTTCTCTCa < 1:1373623/69‑1 (MQ=255) gACGCGCAGCCGCACAGCATTAATCTGACGCAATTACCCATCAGCGAGGCTGATCGGCTTTTTCTCTCa < 1:3331295/69‑1 (MQ=255) gACGCGCAGCCGCACAGCATTAATCTGACGCAATTACCCATCAGCGAGGCTGATCGGCTTTTTCTCTCa < 1:3007748/69‑1 (MQ=255) gACGCGCAGCCGCACAGCATTAATCTGACGCAATTACCCATCAGCGAGGCTGATCGGCTTTTTCTCTCa < 1:848753/69‑1 (MQ=255) gACGCGCAGCCGCACAGCATTAATCTGACGCAATTACCCATCAGCGAGGCTGATCGGCTTTTTCTCTCa < 1:400596/69‑1 (MQ=255) gACGCGCAGCCGCACAGCATTAATCTGACGCAATTACCCATCAGCGAGGCTGATCGGCTTTTTCTCTCa < 1:1726148/69‑1 (MQ=255) gACGCGCAGCCGCACAGCATTAATCTGACGCAATTACCCATCAGCGAGGCTGATCGGCTTTTTCTCTCa < 1:2598218/69‑1 (MQ=255) gACGCGCAGCCGCACAGCATTAATCTGACGCAATTACCCATCAGCGAGGCTGATCGGCTTTTTCTCTCa < 1:1760756/69‑1 (MQ=255) gACGCGCAGCCGCACAGCATTAATCTGACGCAATTACCCATCAGCGAGGCTGATCGGCTTTTTCTCTCa < 1:1144259/69‑1 (MQ=255) gACGCGCAGCCGCACAGCATTAATCTGACGCAATTACCCATCAGCGAGGCTGATCGGCTTTTTCTCTCa < 1:2417633/69‑1 (MQ=255) gACGCGCAGCCGCACAGCATTAATCTGACGCAATTACCCATCAGCGAGGCTGATCGGCTTTTTCTCTCa < 1:2326417/69‑1 (MQ=255) gCCGCACAGCATTAATCTGACGCAATTACCCATCAGCGAGGCTGATCGGCTTTTTCTCTCACGTctct > 1:162879/1‑68 (MQ=255) gCCGCACAGCATTAATCTGACGCAATTACCCATCAGCGAGGCTGATCGGCTTTTTCTCTCACGTCTCtg > 1:747118/1‑69 (MQ=255) gCCGCACAGCATTAATCTGACGCAATTACCCATCAGCGAGGCTGATCGGCTTTTTCTCTCACGTCTCtg > 1:2056288/1‑69 (MQ=255) gCCGCACAGCATTAATCTGACGCAATTACCCATCAGCGAGGCTGATCGGCTTTTTCTCTCACGTCTCtg > 1:2642852/1‑69 (MQ=255) gCCGCACAGCATTAATCTGACGCAATTACCCATCAGCGAGGCTGATCGGCTTTTTCTCTCACGTCTCtg > 1:1714688/1‑69 (MQ=255) gCCGCACAGCATTAATCTGACGCAATTACCCATCAGCGAGGCTGATCGGCTTTTTCTCTCACGTCTCtg > 1:1885879/1‑69 (MQ=255) gCCGCACAGCATTAATCTGACGCAATTACCCATCAGCGAGGCTGATCGGCTTTTTCTCTCACGTCTCtg > 1:2140024/1‑69 (MQ=255) gCATTAATCTGACGCAATTACCCATCAGCGAGGCTGATCGGCTTTTTCTCTCACGTCTCTGTGGGCCg > 1:1293087/1‑68 (MQ=255) gCATTAATCTGACGCAATTACCCATCAGCGAGGCTGATCGGCTTTTTCTCTCACGTCTCTGTGGGCCg > 1:15786/1‑68 (MQ=255) gCATTAATCTGACGCAATTACCCATCAGCGAGGCTGATCGGCTTTTTCTCTCACGTCTCTGTGGGCCg > 1:3199430/1‑68 (MQ=255) gCATTAATCTGACGCAATTACCCATCAGCGAGGCTGATCGGCTTTTTCTCTCACGTCTCTGTGGGCCg > 1:3121724/1‑68 (MQ=255) gCATTAATCTGACGCAATTACCCATCAGCGAGGCTGATCGGCTTTTTCTCTCACGTCTCTGTGGGCCg > 1:1635813/1‑68 (MQ=255) cATTAATCTGACTCAATTACCCATCAGCGAAGCTGACCGGCTTTTtctc > 1:83642/1‑49 (MQ=37) cATTAATCTGACTCAATTACCCATCAGCGAAGCTGACCGGCTTTTtctc > 1:1875369/1‑49 (MQ=37) cATTAATCTGACTCAATTACCCATCAGCGAAGCTGACCGGCTTTTtctc > 1:1364616/1‑49 (MQ=37) cATTAATCTGACTCAATTACCCATCAGCGAAGCTGACCGGCTTTTtctc > 1:2076962/1‑49 (MQ=37) cATTAATCTGACTCAATTACCCATCAGCGAAGCTGACCGGCTTTTtctc > 1:1859793/1‑49 (MQ=37) aCCCATCAGCGAGGCTGATCGGCTTTTTCTCTCACGTCTCTGTGGGc > 1:466414/1‑47 (MQ=255) aGCGAGGCTGATCGGCTTTTTCTCTCACGTCTCTGTGGGCCGGGAAATATTCAGATTCGTACCATTGGc > 1:590710/1‑69 (MQ=255) aGCGAGGCTGATCGGCTTTTTCTCTCACGTCTCTGTGGGCCGGGAAATATTCAGATTCGTACCATTGGc < 1:738712/69‑1 (MQ=255) aGCGAGGCTGATCGGCTTTTTCTCTCACGTCTCTGTGGGCCGGGAAATATTCAGATTCGTACCATTGGc < 1:416475/69‑1 (MQ=255) aGCGAGGCTGATCGGCTTTTTCTCTCACGTCTCTGTGGGCCGGGAAATATTCAGATTCGTACCATTGGc < 1:2882265/69‑1 (MQ=255) aGCGAGGCTGATCGGCTTTTTCTCTCACGTCTCTGTGGGCCGGGAAATATTCAGATTCGTACCATTGGc < 1:3317556/69‑1 (MQ=255) aGCGAGGCTGATCGGCTTTTTCTCTCACGTCTCTGTGGGCCGGGAAATATTCAGATTCGTACCATTGGc < 1:320304/69‑1 (MQ=255) aGCGAGGCTGATCGGCTTTTTCTCTCACGTCTCTGTGGGCCGGGAAATATTCAGATTCGTACCATTGGc < 1:1664980/69‑1 (MQ=255) aGCGAGGCTGATCGGCTTTTTCTCTCACGTCTCTGTGGGCCGGGAAATATTCAGATTCGTACCATTGGc < 1:2719868/69‑1 (MQ=255) aGCGAGGCTGATCGGCTTTTTCTCTCACGTCTCTGTGGGCCGGGAAATATTCAGATTCGTACCATTGGc > 1:1785809/1‑69 (MQ=255) aGCGAGGCTGATCGGCTTTTTCTCTCACGTCTCTGTGGGCCGGGAAATATTCAGATTCGTACCATTGGc > 1:1838803/1‑69 (MQ=255) aGCGAGGCTGATCGGCTTTTTCTCTCACGTCTCTGTGGGCCGGGAAATATTCAGATTCGTACCATTGGc > 1:1941565/1‑69 (MQ=255) aGCGAGGCTGATCGGCTTTTTCTCTCACGTCTCTGTGGGCCGGGAAATATTCAGATTCGTACCAATGGc > 1:1045802/1‑69 (MQ=255) aGCGAGGCTGATCGGCTTTTTCTCTCACGTCTCTATGGGCCGGGAAATATTCAGATTCGTACCATTGGc < 1:1299035/69‑1 (MQ=255) agCTGACCGGCTTTTTCTCTCACGTCTCTGTGGGCCggg > 1:2831162/2‑39 (MQ=37) agCTGACCGGCTTTTTCTCTCACGTCTCTGTGGGCCGGGa > 1:975441/2‑40 (MQ=38) agCTGACCGGCTTTTTCTCTCACGTCTCTGTGGGCCGGGa > 1:921319/2‑40 (MQ=38) agCTGACCGGCTTTTTCTCTCACGTCTCTGTGGGCCGGGa > 1:702531/2‑40 (MQ=38) agCTGACCGGCTTTTTCTCTCACGTCTCTGTGGGCCGGGa > 1:449828/2‑40 (MQ=38) agCTGACCGGCTTTTTCTCTCACGTCTCTGTGGGCCGGGa > 1:3104742/2‑40 (MQ=38) agCTGACCGGCTTTTTCTCTCACGTCTCTGTGGGCCGGGa > 1:1632107/2‑40 (MQ=38) agCTGACCGGCTTTTTCTCTCACGTCTCTGTGGGCCGGGa > 1:2131597/2‑40 (MQ=38) agCTGACCGGCTTTTTCTCTCACGTCTCTGTGGGCCGGGa > 1:2118493/2‑40 (MQ=38) agCTGACCGGCTTTTTCTCTCACGTCTCTGTGGGCCGGGa > 1:1961590/2‑40 (MQ=38) tGATCGGCTTTTTCTCTCACGTCTCTGTGGGCCGGGAAATATTCAGATTCGTACCATTGGc < 1:2478130/61‑1 (MQ=255) | GTACGTAACCCCGACGCGCAGCCGCACAGCATTAATCTGACGCAATTACCCATCAGCGAGGCTGATCGGCTTTTTCTCTCACGTCTCTGTGGGCCGGGAAATATTCAGATTCGTACCATTGGC > minE/693979‑694101 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |