| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 697,873 | C→T | 28.1% | R186R (CGC→CGT) | appA → | phosphoanhydride phosphorylase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 697,873 | 0 | C | T | 28.1% | 83.7 / 31.2 | 78 | R186R (CGC→CGT) | appA | phosphoanhydride phosphorylase |
| Reads supporting (aligned to +/- strand): ref base C (42/14); new base T (15/7); total (57/21) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 5.78e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
GCGATCCTCAGCAGGGCAGGAGGGTCAATTGCTGACTTTACCGGGCATCGGCAAACGGCGTTTCGCGAACTGGAACGGGTGCTTAATTTTCCGCAATCAAACTTGTGCCTTAAACGTGAGAAACAG > minE/697808‑697933 | gcgATCCTCAGCAGGGCAGGAGGGTCAATTGCTGACTTTACCGGGCATCGGCAAACGGCGTTTcgcg < 1:1860416/67‑1 (MQ=255) cTCAGCAGGGCAGGAGGGTCAATTGCTGCCTTTACCGGGCATCGGCAAACGGCGTTTCGTGAGCTGGaa > 1:2617760/1‑69 (MQ=255) cTCAGCAGGGCAGGAGGGTCAATTGCTGACTTTACCGTGCATCGGCAAACGGCGTTTCGTGAACTGGaa > 1:1154129/1‑69 (MQ=255) cTCAGCAGGGCAGGAGGGTCAATTGCTGACTTTACCGGGCATCGGCAAACGGCGTTTCGTGAACTGGaa > 1:1009985/1‑69 (MQ=255) cTCAGCAGGGCAGGAGGGTCAATTGCTGACTTTACCGGGCATCGGCAAACGGCGTTTCGTGAACTGGaa > 1:3277844/1‑69 (MQ=255) cTCAGCAGGGCAGGAGGGTCAATTGCTGACTTTACCGGGCATCGGCAAACGGCGTTTCGTGAACTGGaa > 1:3248346/1‑69 (MQ=255) cTCAGCAGGGCAGGAGGGTCAATTGCTGACTTTACCGGGCATCGGCAAACGGCGTTTCGTGAACTGGaa > 1:1825873/1‑69 (MQ=255) cTCAGCAGGGCAGGAGGGTCAATTGCTGACTTTACCGGGCATCGGCAAACGGCGTTTCGTGAACTGGaa > 1:1738859/1‑69 (MQ=255) cTCAGCAGGGCAGGAGGGTCAATTGCTGACTTTACCGGGCATCGGCAAACGGCGTTTCGTGAACTGGaa > 1:3038045/1‑69 (MQ=255) cTCAGCAGGGCAGGAGGGTCAATTGCTGACTTTACCGGGCATCGGCAAACGGCGTTTCGTGAACTGGaa > 1:2451728/1‑69 (MQ=255) cTCAGCAGGGCAGGAGGGTCAATTGCTGACTTTACCGGGCATCGGCAAACGGCGTTTCGTGAACTGGaa > 1:552905/1‑69 (MQ=255) cTCAGCAGGGCAGGAGGGTCAATTGCTGACTTTACCGGGCATCGGCAAACGGCGTTTCGTGAACTGGaa > 1:562904/1‑69 (MQ=255) cTCAGCAGGGCAGGAGGGTCAATTGCTGACTTTACCGGGCATCGGCAAACGGCGTTTCGTGAACTGGaa > 1:1562131/1‑69 (MQ=255) cTCAGCAGGGCAGGAGGGTCAATTGCTGACTTTACCGGGCATCGGCAAACGGCGTTTCGTGAACTGGaa > 1:27981/1‑69 (MQ=255) cTCAGCAGGGCAGGAGGGTCAATTGCTGACTTTACCGGGCATCGGCAAACGGCGTTTCGTGAACTGGaa > 1:1477080/1‑69 (MQ=255) cTCAGCAGGGCAGGAGGGTCAATTGCTGACTTTACCGGGCATCGGCAAACGGCGTTTCGTGAACTGGaa > 1:2728747/1‑69 (MQ=255) agggcaggAGGGTCAATTGCTGACTTTACCGGGCATCGGCAAACGGCGTTTCGCGAACTGGAACGGGTg > 1:1562458/1‑69 (MQ=255) agggcaggAGGGTCAATTGCTGACTTTACCGGGCATCGGCAAACGGCGTTTCGCGAACTGGAACGGGTg > 1:2737619/1‑69 (MQ=255) gggcaggAGGGTCAATTGCTGACTTTACCGGGCATCGGCAAACGGCGTTTCGTGAACTGGAACGGGTGc < 1:1635601/69‑1 (MQ=255) gggcaggAGGGTCAATTGCTGACTTTACCGGGCATCGGCAAACGGCGTTTCGTGAACTGGAACGGGTGc < 1:2612187/69‑1 (MQ=255) gggcaggAGGGTCAATTGCTGACTTTACCGGGCATCGGCAAACGGCGTTTCGTGAACTGGAACGGGTGc < 1:2546310/69‑1 (MQ=255) gggcaggAGGGTCAATTGCTGACTTTACCGGGCATCGGCAAACGGCGTTTCGTGAACTGGAACGGGTGc < 1:1525348/69‑1 (MQ=255) gggcaggAGGGTCAATTGCTGACTTTACCGGGCATCGGCAAACGGCGTTTCGTGAACTGGAACGGGTGc < 1:277904/69‑1 (MQ=255) ggcaggAGGGTCAATTGCTGACTTTACCGGGCATCGGCAAACGGCGTTTCGTGAACTGGAACGGGTGc < 1:2168122/68‑1 (MQ=255) ggcaggAGGGTCAATTGCTGACTTTACCGGGCATCGGCAAACGGCGTTTCGTGAACTGGAACGGGTGc < 1:2758656/68‑1 (MQ=255) caggAGGGTCAATTGCTGACTTTACCGGGCATCGGCAAACGGCGTTTCGCGAACTGGAACGGGTGCTTa > 1:1367855/1‑69 (MQ=255) aaTTGCTGACTTTACCGGGCATCGGCAAACGGCGTTTCGCGAACTGGAACGGGTGCTTAATTTTCCGCa > 1:1833067/1‑69 (MQ=255) aaTTGCTGACTTTACCGGGCATCGGCAAACGGCGTTTCGCGAACTGGAACGGGTGCTTAATTTTCCGCa > 1:2015499/1‑69 (MQ=255) aaTTGCTGACTTTACCGGGCATCGGCAAACGGCGTTTCGCGAACTGGAACGGGTGCTTAATTTTCCGCa > 1:2393372/1‑69 (MQ=255) aaTTGCTGACTTTACCGGGCATCGGCAAACGGCGTTTCGCGAACTGGAACGGGTGCTTAATTTTCCGCa > 1:182909/1‑69 (MQ=255) aaTTGCTGACTTTACCGGGCATCGGCAAACGGCGTTTCGCGAACTGGAACGGGTGCTTAATTTTCCGCa > 1:306764/1‑69 (MQ=255) aaTTGCTGACTTTACCGGGCATCGGCAAACGGCGTTTCGCGAACTGGAACGGGTGCTTAATTTTCCGCa > 1:2248442/1‑69 (MQ=255) aaTTGCTGACTTTACCGGGCATCGGCAAACGGCGTTTCGCGAACTGGAACGGGTGCTTAATTTTCCGCa > 1:598148/1‑69 (MQ=255) gCTGACTTTACCGGGCATCGGCAAACGGCGTTTCGCGAACTGGAACGGGTGCTTAATTTTCCGCAATCa > 1:1687096/1‑69 (MQ=255) gCTGACTTTACCGGGCATCGGCAAACGGCGTTTCGCGAACTGGAACGGGTGCTTAATTTTCCGCAATCa > 1:229482/1‑69 (MQ=255) gCTGACTTTACCGGGCATCGGCAAACGGCGTTTCGCGAACTGGAACGGGTGCTTAATTTTCCGCAATCa > 1:2268021/1‑69 (MQ=255) gCTGACTTTACCGGGCATCGGCAAACGGCGTTTCGCGAACTGGAACGGGTGCTTAATTTTCCGCAATCa > 1:930564/1‑69 (MQ=255) gCTGACTTTACCGGGCATCGGCAAACGGCGTTTCGCGAACTGGAACGGGTGCTTAATTTTCCGCAATCa > 1:2027414/1‑69 (MQ=255) cATCGGCAAACGGCGTTTCGCGAACTGGAACGGGTGCTTAATTTTCCGCAATCAAACTTGTGCCTTaaa > 1:2238390/1‑69 (MQ=255) cATCGGCAAACGGCGTTTCGCGAACTGGAACGGGTGCTTAATTTTCCGCAATCAAACTTGTGCCTTaaa > 1:2990173/1‑69 (MQ=255) cATCGGCAAACGGCGTTTCGCGAACTGGAACGGGTGCTTAATTTTCCGCAATCAAACTTGTGCCTTaaa > 1:664666/1‑69 (MQ=255) cATCGGCAAACGGCGTTTCGCGAACTGGAACGGGTGCTTAATTTTCCGCAATCAAACTTGTGCCTTaaa > 1:802059/1‑69 (MQ=255) ggaaaaCGGCGTTTCGCGAACTGGAACGGGTGCTTAATTTTCCGCAATCAAACTTGTGCCTTAAAc > 1:1954671/4‑66 (MQ=255) ggCAAACGGCGTTTCGCGAACTGGAACGGGTGCTTAATTTTCCGCAATCAAACTTGTGCCTTaaa > 1:3026471/1‑65 (MQ=255) ggCAAACGGCGTTTCGCGAACTGGAACGGGTGCTTAATTTTCCGCAATCAAACTTGTGCCTTAAAc > 1:2841262/1‑66 (MQ=255) ggCAAACGGCGTTTCGCGAACTGGAACGGGTGCTTAATTTTCCGCAATCAAACTTGTGCCTTAAAc > 1:545352/1‑66 (MQ=255) ggCAAACGGCGTTTCGCGAACTGGAACGGGTGCTTAATTTTCCGCAATCAAACTTGTGCCTTAAAc > 1:3029739/1‑66 (MQ=255) ggCAAACGGCGTTTCGCGAACTGGAACGGGTGCTTAATTTTCCGCAATCAAACTTGTGCCTTAAAc > 1:462693/1‑66 (MQ=255) ggCAAACGGCGTTTCGCGAACTGGAACGGGTGCTTAATTTTCCGCAATCAAACTTGTGCCTTAAAc > 1:3305688/1‑66 (MQ=255) ggCAAACGGCGTTTCGCGAACTGGAACGGGTGCTTAATTTTCCGCAATCAAACTTGTGCCTTAAAc > 1:864610/1‑66 (MQ=255) ggCAAACGGCGTTTCGCGAACTGGAACGGGTGCTTAATTTTCCGCAATCAAACTTGTGCCTTAAAc > 1:3157124/1‑66 (MQ=255) ggCAAACGGCGTTTCGCGAACTGGAACGGGTGCTTAATTTTCCGCAATCAAACTTGTGCCTTAAAc > 1:108482/1‑66 (MQ=255) ggCAAACGGCGTTTCGCGAACTGGAACGGGTGCTTAATTTTCCGCAATCAAACTTGTGCCTTAAAc > 1:1542124/1‑66 (MQ=255) ggCAAACGGCGTTTCGCGAACTGGAACGGGTGCTTAATTTTCCGCAATCAAACTTGTGCCTTAAAc > 1:161187/1‑66 (MQ=255) ggCAAACGGCGTTTCGCGAACTGGAACGGGTGCTTAATTTTCCGCAATCAAACTTGTGCCTTAAAc > 1:1650674/1‑66 (MQ=255) ggCAAACGGCGTTTCGCGAACTGGAACGGGTGCTTAATTTTCCGCAATCAAACTTGTGCCTTAAAc > 1:16882/1‑66 (MQ=255) ggCAAACGGCGTTTCGCGAACTGGAACGGGTGCTTAATTTTCCGCAATCAAACTTGTGCCTTAAAc > 1:1804185/1‑66 (MQ=255) ggCAAACGGCGTTTCGCGAACTGGAACGGGTGCTTAATTTTCCGCAATCAAACTTGTGCCTTAAAc > 1:1876549/1‑66 (MQ=255) ggCAAACGGCGTTTCGCGAACTGGAACGGGTGCTTAATTTTCCGCAATCAAACTTGTGCCTTAAAc > 1:1521531/1‑66 (MQ=255) ggCAAACGGCGTTTCGCGAACTGGAACGGGTGCTTAATTTTCCGCAATCAAACTTGTGCCTTAAAc > 1:1486436/1‑66 (MQ=255) ggCAAACGGCGTTTCGCGAACTGGAACGGGTGCTTAATTTTCCGCAATCAAACTTGTGCCTTAAAc > 1:1351451/1‑66 (MQ=255) ggCAAACGGCGTTTCGCGAACTGGAACGGGTGCTTAATTTTCCGCAATCAAACTTGTGCCTTAAAc > 1:224419/1‑66 (MQ=255) aaaCGGCGTTTCTCGAACTGGAACGGGTGCTTAATTTTCCGCAATCAAACTTGTGCCTTAAACGTgaga < 1:2462250/69‑1 (MQ=255) aaaCGGCGTTTCGCGAACTGGAACGGGTGCTTAATTTTCCGCAATCAAACTTGTGCCTTAAACGTgaga < 1:2088903/69‑1 (MQ=255) aaaCGGCGTTTCGCGAACTGGAACGGGTGCTTAATTTTCCGCAATCAAACTTGTGCCTTAAACGTgaga < 1:2173262/69‑1 (MQ=255) aaaCGGCGTTTCGCGAACTGGAACGGGTGCTTAATTTTCCGCAATCAAACTTGTGCCTTAAACGTgaga < 1:2217432/69‑1 (MQ=255) aaaCGGCGTTTCGCGAACTGGAACGGGTGCTTAATTTTCCGCAATCAAACTTGTGCCTTAAACGTgaga < 1:389924/69‑1 (MQ=255) aaaCGGCGTTTCGCGAACTGGAACGGGTGCTTAATTTTCCGCAATCAAACTTGTGCCTTAAACGTgaga < 1:390542/69‑1 (MQ=255) aaaCGGCGTTTCGCGAACTGGAACGGGTGCTTAATTTTCCGCAATCAAACTTGTGCCTTAAACGTgaga < 1:394520/69‑1 (MQ=255) aaaCGGCGTTTCGCGAACTGGAACGGGTGCTTAATTTTCCGCAATCAAACTTGTGCCTTAAACGTgaga < 1:1681848/69‑1 (MQ=255) aaaCGGCGTTTCGCGAACTGGAACGGGTGCTTAATTTTCCGCAATCAAACTTGTGCCTTAAACGTgaga < 1:892061/69‑1 (MQ=255) aaCGGCGTTTCGCGAACTGGAACGGGTGCTTAATTTTCCGCAATCAAACTTGTGCCTTAAACGTgaga < 1:959743/68‑1 (MQ=255) cGGCGTTTCGCGAACTGGAACGGGTGCTTAATTTTCCGCAAt > 1:2028109/1‑42 (MQ=255) cGGCGTTTCGCGAACTGGAACGGGTGCTTAATTTTCCGCAATCAAACTTGTGCCTTAAACGTGAGAAAc > 1:3211205/1‑69 (MQ=255) cGGCGTTTCGCGAACTGGAACGGGTGCTTAATTTTCCGCAATCAAACTTGTGCCTTAAACGTGAGAAAc > 1:1293687/1‑69 (MQ=255) gCGTTTCGCGAACTGGAACGGGTGCTTAATTTTCCGCAATCAAACTTGTGCCTTAAACGTGAGAAACAg < 1:2157583/69‑1 (MQ=255) gCGTTTCGCGAACTGGAACGGGTGCTTAATTTTCCGCAATCAAACTTGTGCCTTAAACGTGAGAAACAg < 1:2173438/69‑1 (MQ=255) gCGTTTCGCGAACTGGAACGGGTGCTTAATTTTCCGCAATCAAACTTGTGCCTTAAACGTGAGAAACAg < 1:2650501/69‑1 (MQ=255) tttCGCGAACTGGAACGGGTGCTTAATTTTCCGCAATCAAACTTGTGCCTTAAACGTgaga < 1:2711023/61‑1 (MQ=255) | GCGATCCTCAGCAGGGCAGGAGGGTCAATTGCTGACTTTACCGGGCATCGGCAAACGGCGTTTCGCGAACTGGAACGGGTGCTTAATTTTCCGCAATCAAACTTGTGCCTTAAACGTGAGAAACAG > minE/697808‑697933 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |