| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 709,461 | T→A | 41.3% | A138A (GCA→GCT) | yceJ ← | predicted cytochrome b561 |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 709,461 | 0 | T | A | 41.3% | 19.4 / 43.9 | 46 | A138A (GCA→GCT) | yceJ | predicted cytochrome b561 |
| Reads supporting (aligned to +/- strand): ref base T (10/17); new base A (6/13); total (16/30) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 7.62e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
AATCCGTGCATAACGGACAGTACTACGACGCTCCACGCGAGCCAAAAATGCAGGGCACCGGCAAAGTCTGCCTGTGCGCCAGCGTCGGCAAGGGTCGCGGGGACGTCAAACCAGCCAAAAACGCTGATCGGTTTACC > minE/709393‑709529 | aaTCCGTGCATAACGGACAGTACTACGACGCTCCACGCGAGCCAAAAATGCAGGGCACCGGCAAAGTCt > 1:1447252/1‑69 (MQ=255) ccGTGCATAACGGACAGTACTACGACGCTCCACGCGAGCCAAAAATGCAGGGCACCGGCAAAGTCTGcc > 1:1179173/1‑69 (MQ=255) ccGTGCATAACGGACAGTACTACGACGCTCCACGCGAGCCAAAAATGCAGGGCACCGGCAAAGTCTGcc > 1:2625744/1‑69 (MQ=255) ccGTGCATAACGGACAGTACTACGACGCTCCACGCGAGCCAAAAATGCAGGGCACCGGCAAAGTCTGcc > 1:702801/1‑69 (MQ=255) tAACGGACAGTACTACAACGCTCCACGCGAGCCAAAAATGCAGGGCACCGGCAAAGTCAGCCTGTgcg < 1:958061/68‑1 (MQ=255) tAACGGACAGTACTACAACGCTCCACGCGAGCCAAAAATGCAGGGCACCGGCAAAGTCAGCCTGTgcg < 1:1245504/68‑1 (MQ=255) tAACGGACAGTACTACAACGCTCCACGCGAGCCAAAAATGCAGGGCACCGGCAAAGTCAGCCTGTgcg < 1:3068394/68‑1 (MQ=255) tAACGGACAGTACTACAACGCTCCACGCGAGCCAAAAATGCAGGGCACCGGCAAAGTCAGCCTGTgcg < 1:3051126/68‑1 (MQ=255) tAACGGACAGTACTACAACGCTCCACGCGAGCCAAAAATGCAGGGCACCGGCAAAGTCAGCCTGTgcg < 1:1102905/68‑1 (MQ=255) tAACGGACAGTACTACAACGCTCCACGCGAGCCAAAAATGCAGGGCACCGGCAAAGTCAGCCTGTgcg < 1:2967476/68‑1 (MQ=255) tAACGGACAGTACTACAACGCTCCACGCGAGCCAAAAATGCAGGGCACCGGCAAAGTCAGCCTGTgcg < 1:2751189/68‑1 (MQ=255) tAACGGACAGTACTACAACGCTCCACGCGAGCCAAAAATGCAGGGCACCGGCAAAGTCAGCCTGTgcg < 1:2701216/68‑1 (MQ=255) tAACGGACAGTACTACAACGCTCCACGCGAGCCAAAAATGCAGGGCACCGGCAAAGTCAGCCTGTgcg < 1:1822345/68‑1 (MQ=255) tAACGGACAGTACTACAACGCTCCACGCGAGCCAAAAATGCAGGGCACCGGCAAAGTCAGCCTGTgcg < 1:2461190/68‑1 (MQ=255) tAACGGACAGTACTACAACGCTCCACGCGAGCCAAAAATGCAGGGCACCGGCAAAGTCAGCCTGTgcg < 1:2443049/68‑1 (MQ=255) tAACGGACAGTACTACAACGCTCCACGCGAGCCAAAAATGCAGGGCACCGGCAAAGTCAGCCTGTgcg < 1:2290649/68‑1 (MQ=255) aaCGGACAGTACTACAACGCTCCACGCGAGCCAAAAATGCAGGGCACCGGCAAAGTCAGCCTGTgcg < 1:700334/67‑1 (MQ=255) aGTACTACGACGCTCCACGCGAGCCAAAAATGCAGGGCACCGGCAAAGTCTTCCTGTGCGCCAGCGTCg < 1:962457/69‑1 (MQ=255) aGTACTACGACGCTCCACGCGAGCCAAAAATGCAGGGCACCGGCAAAGTCTGCCTGTGCGCCAGCGTCg < 1:74133/69‑1 (MQ=255) aGTACTACGACGCTCCACGCGAGCCAAAAATGCAGGGCACCGGCAAAGTCTGCCTGTGCGCCAGCGTCg < 1:1731318/69‑1 (MQ=255) aGTACTACGACGCTCCACGCGAGCCAAAAATGCAGGGCACCGGCAAAGTCTGCCTGTGCGCCAGCGTCg < 1:2934752/69‑1 (MQ=255) gTACTACGACGCTCCACGCGAGCCAAAAATGCAGGGCACCGGCAAAGTCTGCCTGTGCGCCAGCGTCg < 1:2029164/68‑1 (MQ=255) gTACTACGACGCTCCACGCGAGCCAAAAATGCAGGGCACCGGCAAAGTCTGCCTGTGCGCCAGCGTCg < 1:1298941/68‑1 (MQ=255) acgacgCTCCACGCGAGCCAAAAATGCAGGGCACCGGCAAAGTCTGCCTGTGCGCCAGCGTCGGCATgg > 1:353620/1‑69 (MQ=255) acgacgCTCCACGCGAGCCAAAAATGCAGGGCACCGGCAAAGTCTGCCTGTGCGCCAGCGTCGGCAAgg > 1:637707/1‑69 (MQ=255) acgacgCTCCACGCGAGCCAAAAATGCAGGGCACCGGCAAAGTCTGCCTGTGCGCCAGCGTCGGCAAgg > 1:1473962/1‑69 (MQ=255) acgacgCTCCACGCGAGCCAAAAATGCAGGGCACCGGCAAAGTCTGCCTGTGCGCCAGCGTCGGCAAgg > 1:575131/1‑69 (MQ=255) acgacgCTCCACGCGAGCCAAAAATGCAGGGCACCGGCAAAGTCTGCCTGTGCGCCAGCGTCGGCAAgg > 1:3279562/1‑69 (MQ=255) cgCTCCACGCGAGCCAAAAATGCAGGGCACCGGCAAAGTCAGCCTGTGCGCCAGCGTCGGAGAGGATcg > 1:2244246/1‑69 (MQ=255) cgCTCCACGCGAGCCAAAAATGCAGGGCACCGGCAAAGTCAGCCTGTGCGCCAGCGTCGGAGAGGATcg > 1:724456/1‑69 (MQ=255) cgCTCCACGCGAGCCAAAAATGCAGGGCACCGGCAAAGTCAGCCTGTGCGCCAGCGTCGGAGAGGATcg > 1:1348251/1‑69 (MQ=255) cgCTCCACGCGAGCCAAAAATGCAGGGCACCGGCAAAGTCAGCCTGTGCGCCAGCGTCGGAGAGGATcg > 1:623123/1‑69 (MQ=255) cgCTCCACGCGAGCCAAAAATGCAGGGCACCGGCAAAGTCAGCCTGTGCGCCAGCGTCGGAGAGGATcg > 1:3415028/1‑69 (MQ=255) cgCTCCACGCGAGCCAAAAATGCAGGGCACCGGCAAAGTCAGCCTGTGCGCCAGCGTCGGAGAGGATcg > 1:2678647/1‑69 (MQ=255) aGCCAAAAATGCAGGGCACCGGCAAAGTCTGCCTGTGCGCCAGCGTCGGCAAGGGTCGCGGGGACGTCa < 1:126850/69‑1 (MQ=255) ggCACCGGCAAAGTCTGCCTGTGCTCCAGCGTCGGCAAGGGTCGCGGGGACGTCAAACCAGCCAAAAAc < 1:1690657/69‑1 (MQ=255) ggCACCGGCAAAGTCTGCCTGTGCGCCAGCGTCGGCAAGGGTCGCGGGGACGTCAAACCAGCCAAAAAc < 1:3091128/69‑1 (MQ=255) ggCACCGGCAAAGTCTGCCTGTGCGCCAGCGTCGGCAAGGGTCGCGGGGACGTCAAACCAGCCAAAAAc < 1:3425659/69‑1 (MQ=255) ggCACCGGCAAAGTCTGCCTGTGCGCCAGCGTCGGCAAGGGTCGCGGGGACGTCAAACCAGCCAAAAAc < 1:1414987/69‑1 (MQ=255) ggCACCGGCAAAGTCTGCCTGTGCGCCAGCGTCGGCAAGGGTCGCGGGGACGTCAAACCAGCCAAAAAc < 1:672129/69‑1 (MQ=255) ggCACCGGCAAAGTCTGCCTGTGCGCCAGCGTCGGCAAGGGTCGCGGGGACGTCAAACCAGCCAAAAAc < 1:2648935/69‑1 (MQ=255) ggCACCGGCAAAGTCTGCCTGTGCGCCAGCGTCGGCAAGGGTCGCGGGGACGTCAAACCAGCCAAAAAc < 1:2641088/69‑1 (MQ=255) ggCACCGGCAAAGTCTGCCTGTGCGCCAGCGTCGGCAAGGGTCGCGGGGACGTCAAACCAGCCAAAAAc < 1:2367157/69‑1 (MQ=255) ggCACCGGCAAAGTCTGCCTGTGCGCCAGCGTCGGCAAGGGTCGCGGGGACGTCAAACCAGCCAAAAAc < 1:787343/69‑1 (MQ=255) ggCACCGGCAAAGTCTGCCTGTGCGCCAGCGTCGGCAAGGGTCGCGGGGACGTCAAACCAGCCAAAAAc < 1:834216/69‑1 (MQ=255) gCACCGGCAAAGTCTGCCTGTGCGCCAGCGTCGGCAAGGGTCGCGGGGACGTCAAACCCGCCAAAAACg > 1:1195024/1‑69 (MQ=255) gCACCGGCAAAGTCTGCCTGTGCGCCAGCGTCGGCAAGGGTCGCGGGGACGTCAAACCAGCCAAAAACg > 1:1851820/1‑69 (MQ=255) tGCCTGTGCGCCAGCGTCGGCAAGGGTCGCGGGGACGTCAAACCAGCCAAAACCGCTGATCGGTTTAcc > 1:14251/1‑69 (MQ=255) | AATCCGTGCATAACGGACAGTACTACGACGCTCCACGCGAGCCAAAAATGCAGGGCACCGGCAAAGTCTGCCTGTGCGCCAGCGTCGGCAAGGGTCGCGGGGACGTCAAACCAGCCAAAAACGCTGATCGGTTTACC > minE/709393‑709529 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |