| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 711,123 | T→C | 37.5% | T98A (ACA→GCA) | solA ← | N‑methyltryptophan oxidase, FAD‑binding |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 711,123 | 0 | T | C | 37.5% | 23.2 / 28.9 | 48 | T98A (ACA→GCA) | solA | N‑methyltryptophan oxidase, FAD‑binding |
| Reads supporting (aligned to +/- strand): ref base T (20/10); new base C (4/14); total (24/24) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.48e-03 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.99e-01 | |||||||||||
TCGAGCTTTTCAACGTTGAGTTGCCATTGTTCGGCGCTGTGGGCGACGTTGGCGAGAAATGTGGAATCAGCCGGGCCAAGGTTAATGACGCCAGAGCGTACAAAAATGGGATCTTCTTCGTTGTGGCGG > minE/711062‑711190 | tCGAGCTTTTTAACGTTGAGTTGCCATTGTTCGGCGCTGTGGGCGACGTTGGCGAGAAATGTGGAATCa > 1:1222167/1‑69 (MQ=255)tCGAGCTTTTCAACGTTGAGTTGCCATTGTTCGGCGCTGTGGGCGACGTTGGCGAGAAATGTGGAATCa > 1:1128223/1‑69 (MQ=255)tCGAGCTTTTCAACGTTGAGTTGCCATTGTTCGGCGCTGTGGGCGACGTTGGCGAGAAATGTGGAATCa > 1:1145706/1‑69 (MQ=255)tCGAGCTTTTCAACGTTGAGTTGCCATTGTTCGGCGCTGTGGGCGACGTTGGCGAGAAATGTGGAATCa > 1:3361276/1‑69 (MQ=255)tCGAGCTTTTCAACGTTGAGTTGCCATTGTTCGGCGCTGTGGGCGACGTTGGCGAGAAATGTGGAATCa > 1:3114483/1‑69 (MQ=255)tCGAGCTTTTCAACGTTGAGTTGCCATTGTTCGGCGCTGTGGGCGACGTTGGCGAGAAATGTGGAATCa > 1:1828784/1‑69 (MQ=255) gAGTTGCCATTGTTCGGCGCTGTGGGCGACGTTGGCGAGAAATGTGGAATCAGCCTGGCCAAGGTTAAt < 1:1713897/69‑1 (MQ=255) gAGTTGCCATTGTTCGGCGCTGTGGGCGACGTTGGCGAGAAATGTGGAATCAGCCGGGCCAAGGTTAAt < 1:1728315/69‑1 (MQ=255) gAGTTGCCATTGTTCGGCGCTGTGGGCGACGTTGGCGAGAAATGTGGAATCAGCCGGGCCAAGGTTAAt < 1:1470783/69‑1 (MQ=255) gAGTTGCCATTGTTCGGCGCTGTGGGCGACGTTGGCGAGAAATGTGGAATCAGCCGGGCCAAGGTTAAt < 1:1804041/69‑1 (MQ=255) gAGTTGCCACTGTTCGGCGCTGTGGGCGACGTTGGCGAGAAATGCGGAGTCAGCCGGACCGAGGTTAAt < 1:2706738/69‑1 (MQ=255) gAGTTGCCACTGTTCGGCGCTGTGGGCGACGTTGGCGAGAAATGCGGAGTCAGCCGGACCGAGGTTAAt < 1:3045072/69‑1 (MQ=255) gAGTTGCCACTGTTCGGCGCTGTGGGCGACGTTGGCGAGAAATGCGGAGTCAGCCGGACCGAGGTTAAt < 1:2841336/69‑1 (MQ=255) gAGTTGCCACTGTTCGGCGCTGTGGGCGACGTTGGCGAGAAATGCGGAGTCAGCCGGACCGAGGTTAAt < 1:304587/69‑1 (MQ=255) gAGTTGCCACTGTTCGGCGCTGTGGGCGACGTTGGCGAGAAATGCGGAGTCAGCCGGACCGAGGTTAAt < 1:185930/69‑1 (MQ=255) gAGTTGCCACTGTTCGGCGCTGTGGGCGACGTTGGCGAGAAATGCGGAGTCAGCCGGACCGAGGTTAAt < 1:1885946/69‑1 (MQ=255) gAGTTGCCACTGTTCGGCGCTGTGGGCGACGTTGGCGAGAAATGCGGAGTCAGCCGGACCGAGGTTAAt < 1:2565831/69‑1 (MQ=255) gAGTTGCCACTGTTCGGCGCTGTGGGCGACGTTGGCGAGAAATGCGGAGTCAGCCGGACCGAGGTTAAt < 1:249056/69‑1 (MQ=255) gAGTTGCCACTGTTCGGCGCTGTGGGCGACGTTGGCGAGAAATGCGGAGTCAGCCGGACCGAGGTTAAt < 1:1721927/69‑1 (MQ=255) gAGTTGCCACTGTTCGGCGCTGTGGGCGACGTTGGCGAGAAATGCGGAGTCAGCCGGACCGAGGTTAAt < 1:1434624/69‑1 (MQ=255) gAGTTGCCACTGTTCGGCGCTGTGGGCGACGTTGGCGAGAAATGCGGAGTCAGCCGGACCGAGGTTAAt < 1:1356903/69‑1 (MQ=255) gAGTTGCCACTGTTCGGCGCTGTGGGCGACGTTGGCGAGAAATGCGGAGTCAGCCGGACCGAGGTTAAt < 1:1235327/69‑1 (MQ=255) gAGTTGCCACTGTTCGGCGCTGTGGGCGACGTTGGCGAGAAATGCGGAGTCAGCCGGACCGAGGTTAAt < 1:517966/69‑1 (MQ=255) gAGTTGCCACTGTTCGGCGCTGTGGGCGACGTTGGCGAGAAATGCGGAGTCAGCCGGACCGAGGTTAAt < 1:736621/69‑1 (MQ=255) ttGCCATTGTTCGGCGCTGTGGGCGACGTTGGCGAGAAATGTGGAATCAGCCGGGCCAAGGTTAATGAc > 1:2910220/1‑69 (MQ=255) ttGCCATTGTTCGGCGCTGTGGGCGACGTTGGCGAGAAATGTGGAATCAGCCGGGCCAAGGTTAATGAc > 1:1446687/1‑69 (MQ=255) gTTCGGCGCTGTGGGCGACGTTGGCGAGAAATGTGGAATCAGCCGGGCCAAGGTTAATGACGCCagag > 1:2941748/1‑68 (MQ=255) gTTCGGCGCTGTGGGCGACGTTGGCGAGAAATGTGGAATCAGCCGGGCCAAGGTTAATGACGCCagag > 1:1462301/1‑68 (MQ=255) gTTCGGCGCTGTGGGCGACGTTGGCGAGAAATGTGGAATCAGCCGGGCCAAGGTTAATGACGCCagag > 1:2162732/1‑68 (MQ=255) ggCGACGTTGGCGAGAAATGTGGAATCAGCCGGGCCAAGGTTAATGACGCCAGAGCGTACAAAAATggg < 1:1059978/69‑1 (MQ=255) ggCGACGTTGGCGAGAAATGTGGAATCAGCCGGGCCAAGGTTAATGACGCCAGAGCGTACAAAAATggg < 1:487201/69‑1 (MQ=255) ggCGACGTTGGCGAGAAATGTGGAATCAGCCGGGCCAAGGTTAATGACGCCAGAGCGTACAAAAATggg < 1:2423460/69‑1 (MQ=255) ggCGACGTTGGCGAGAAATGTGGAATCAGCCGGGCCAAGGTTAATGACGCCAGAGCGTACAAAAATggg < 1:1846877/69‑1 (MQ=255) ggCGACGTTGGCGAGAAATGTGGAATCAGCCGGGCCAAGGTTAATGACGCCAGAGCGTACAAAAATggg < 1:1750019/69‑1 (MQ=255) ggCGACGTTGGCGAGAAATGTGGAATCAGCCGGGCCAAGGTTAATGACGCCAGAGCGTACAAAAATggg < 1:1392887/69‑1 (MQ=255) ggCGACGTTGGCGAGAAATGCGGAGTCAGCCGGACCGAGGTTa > 1:651304/1‑43 (MQ=25) ggCGACGTTGGCGAGAAATGCGGAGTCAGCCGGACCGAGGTTa > 1:2748530/1‑43 (MQ=25) ggCGACGTTGGCGAGAAATGCGGAGTCAGCCGGACCGAGGTTa > 1:1109369/1‑43 (MQ=25) ggCGACGTTGGCGAGAAATGCGGAGTCAGCCGGACCGAGGTTa > 1:3366557/1‑43 (MQ=25) gACGTTGGCGAGAAATGTGGAATCAGCCGGGCCAAGGTTAATGACGCCAGAGCGTACAAAAATGGGAtc > 1:2528474/1‑69 (MQ=255) gACGTTGGCGAGAAATGTGGAATCAGCCGGGCCAAGGTTAATGACGCCAGAGCGTACAAAAATGGGAtc > 1:1695774/1‑69 (MQ=255) ggCGAGAAATGTGGAATCAGCCGGGCCAAGGTTAATGACGCCAGAGCGTACAAAAATGGGATCttcttc > 1:2255308/1‑69 (MQ=255) ggCGAGAAATGTGGAATCAGCCGGGCCAAGGTTAATGACGCCAGAGCGTACAAAAATGGGATCttcttc > 1:839331/1‑69 (MQ=255) ggCGAGAAATGTGGAATCAGCCGGGCCAAGGTTAATGACGCCAGAGCGTACAAAAATGGGATCttcttc > 1:608916/1‑69 (MQ=255) ggCGAGAAATGTGGAATCAGCCGGGCCAAGGTTAATGACGCCAGAGCGTACAAAAATGGGATCttcttc > 1:1244584/1‑69 (MQ=255) ggCGAGAAATGTGGAATCAGCCGGGCCAAGGTTAATGACGCCAGAGCGTACAAAAATGGGATCttcttc > 1:1280387/1‑69 (MQ=255) ggCGAGAAATGTGGAATCAGCCGGGCCAAGGTTAATGACGCCAGAGCGTACAAAAATGGGATCttcttc > 1:1726768/1‑69 (MQ=255) ggCGAGAAATGTGGAATCAGCCGGGCCAAGGTTAATGACGCCAGAGCGTACAAAAATGGGATCttcttc > 1:2612548/1‑69 (MQ=255) gtgGAATCAGCCGGGCCAAGGTTAATGACGCCAGAGCGTACAAAAATGGGATCTTCTTCGTTGTGGCgg < 1:351698/69‑1 (MQ=255) gtgGAATCAGCCGGGCCAAGGTTAATGACGCCAGAGCGTACAAAAATGGGATCTTCTTCGTTGTGGCgg < 1:432860/69‑1 (MQ=255) gtgGAATCAGCCGGGCCAAGGTTAATGACGCCAGAGCGTACAAAAATGGGATCTTCTTCGTTGTGGCgg < 1:1747448/69‑1 (MQ=255) gtgGAATCAGCCGGGCCAAGGTTAATGACGCCAGAGCGTACAAAAATGGGATCTTCTTCGTTGTGGCgg < 1:1775995/69‑1 (MQ=255) gtgGAATCAGCCGGGCCAAGGTTAATGACGCCAGAGCGTACAAAAATGGGATCTTCTTCGTTGTGGCgg < 1:102184/69‑1 (MQ=255) gtgGAATCAGCCGGGCCAAGGTTAATGACGCCAGAGCGTACAAAAATGGGATCTTCTTCGTTGTGGCgg < 1:843732/69‑1 (MQ=255) tgGAATCAGCCGGGCCAAGGTTAATGACGCCAGAGCGTACAAAAATGGGATCTTCTTCGTTGTGGCgg < 1:1954179/68‑1 (MQ=255) | TCGAGCTTTTCAACGTTGAGTTGCCATTGTTCGGCGCTGTGGGCGACGTTGGCGAGAAATGTGGAATCAGCCGGGCCAAGGTTAATGACGCCAGAGCGTACAAAAATGGGATCTTCTTCGTTGTGGCGG > minE/711062‑711190 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |